FlyBase Gene Report: Dmel\CG17124

General Information

Symbol

Dmel\CG17124

Species

D. melanogaster

Name

Annotation Symbol

CG17124

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032297

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

32A5-32A5

Sequence location

2L:10,744,075..10,756,325 [-]

Recombination map

2-43

RefSeq locus

NT_033779 REGION:10744075..10756325

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein serine/threonine phosphatase inhibitor activity

inferred from biological aspect of ancestor with PANTHER:PTN002313545

(GO Reference Genome Project, 2011-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from electronic annotation with InterPro:IPR008025, InterPro:IPR036658

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG17124 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKQ5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG17124-RA

FBtr0080117

NM_135606

1251

124

CG17124-RB

FBtr0080118

NM_164942

2291

124

CG17124-RC

FBtr0340246

NM_001298884

2989

124

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG17124-PA

FBpp0079706

14.6

124

5.81

FBpp0079707

14.6

124

5.81

FBpp0309219

14.6

124

5.81

Q9VKQ5

NP_001285813

AHN54327

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

124 aa isoforms: CG17124-PA, CG17124-PB, CG17124-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VKQ5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG17124 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17124_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.46

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

head visceral muscle primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG17124 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG17124

View exonic expression by tissue for Dmel\CG17124

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032297

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34485/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032297

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032297

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032297

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17124

Flygut - An atlas of the Drosophila adult midgut

FBgn0032297

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG17124

Stages(s) 1-3
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG17124

Transgenic constructs containing regulatory region of CG17124

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel8026

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

CG17124^c01575

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG17124^GD8599

viable, with Scer\GAL4^pnr-MD237

CG17124^GD8599

viable, with Scer\GAL4^tin.CΔ4

CG17124^GD8599

Sterility

Allele

fertile

CG17124^c01575

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\PPP1R14B

11 of 14

Yes

Hsap\PPP1R14A

8 of 14

Yes

Hsap\PPP1R14C

8 of 14

Yes

Hsap\PPP1R14D

6 of 14

Yes

Hsap\RNF146

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Ppp1r14b

11 of 14

Yes

Rnor\RGD1309049

10 of 14

Yes

Rnor\Ppp1r14a

8 of 14

Yes

Rnor\Ppp1r14c

8 of 14

Yes

Rnor\Ppp1r14d

5 of 14

Yes

Rnor\Rnf146

1 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Ppp1r14b

11 of 14

Yes

Mmus\Ppp1r14bl

9 of 14

Yes

Mmus\Ppp1r14a

8 of 14

Yes

Mmus\Ppp1r14c

8 of 14

Yes

Mmus\Ppp1r14d

6 of 14

Yes

Mmus\Rnf146

1 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\ppp1r14a

7 of 13

Yes

Xtro\ppp1r14b

6 of 13

Yes

Xtro\ppp1r14c

5 of 13

Xtro\ppp1r14d

4 of 13

Yes

Xtro\topbp1

1 of 13

Danio rerio (Zebrafish) (7)

Drer\ppp1r14bb

9 of 14

Yes

Drer\ppp1r14d

9 of 14

Yes

Drer\ppp1r14aa

8 of 14

Yes

Drer\ppp1r14ab

8 of 14

Yes

Drer\ppp1r14ba

8 of 14

Yes

Drer\ppp1r14c

8 of 14

Yes

Drer\rnf146

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\F55C10.5

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP007886

11 of 12

Yes

Agam\AgaP_AGAP009902

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG17124. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Rnf146

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPP1R14B; protein phosphatase 1 regulatory inhibitor subunit 14B

11 of 14

601140

PPP1R14A; protein phosphatase 1 regulatory inhibitor subunit 14A

8 of 14

608153

PPP1R14C; protein phosphatase 1 regulatory inhibitor subunit 14C

8 of 14

613242

PPP1R14D; protein phosphatase 1 regulatory inhibitor subunit 14D

6 of 14

613256

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG17124 - Act57B

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - Act5C

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - Df31

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - Nsun2

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - Sumo

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - TER94

experimental knowledge based

(Guruharsha et al., 2011)

CG17124 - capt

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60559

DroID - A comprehensive database of gene and protein interactions.

FBgn0032297

MIST (protein-protein) - An integrated Molecular Interaction Database

34485

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

RHO GTPases activate PKNs

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-43

Cytogenetic map

32A5-32A5

Sequence location

2L:10,744,075..10,756,325 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32A5-32A5

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-43

(FlyBase, 2016)

2-36.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

83075

y¹ v¹; P{WKO.3-G3}attP40

10638

w¹¹¹⁸; PBac{PB}CG17124^c01575

65629

w¹¹¹⁸; PBac{IT.GAL4}CG17124^1240-G4

35123

y¹ w^*; Mi{MIC}CG17124^MI02562/SM6a

NIG-Fly

17124R-1

P{NIG.17124R}1

M2L-0831

y¹ w¹¹¹⁸; CG17124^SK2 P{neoFRT}40A/CyO-Tb

M2L-0832

y¹ w¹¹¹⁸; CG17124^SK8 P{neoFRT}40A/CyO-Tb

2LG-0661

y² VhaAC39-1^cho-1 v¹; P{U6-CG17124.NIG.sgRNA}attP40/CyO

VDRC

v19078

w¹¹¹⁸ P{GD8599}v19078

v341045

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00051}attP40/CyO-GFP

v341233

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00272}attP40/CyO-GFP

Genomic Clones (30)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (70)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE14405

Other clones

DPiM_RE14405

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032297

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032297

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

(Claridge-Chang et al., 2001)

Area matching Drosophila EST AI108647.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG17124 anon-WO0118547.153

(FlyBase, 1996-)

Additional comments

Source for merge of CG17124 anon-WO0118547.153 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG17124

(Burghardt et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Pang et al., 2021, Wu et al., 2020, Meltzer, 2019-, Meltzer et al., 2019, Yildirim et al., 2019, Clandinin and Owens, 2016-, Katsuyama et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Bauer et al., 2010, Buffin and Gho, 2010, Kim and Marqués, 2010, Kong et al., 2010, Fujikawa et al., 2009, Valanne et al., 2007, Wu et al., 2007, Zeitlinger et al., 2007)

anon-WO0118547.153

Name Synonyms

Secondary FlyBase IDs

FBgn0062210

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34485

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKQ5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VKQ5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKQ5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032297

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34485/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032297

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032297

FlyMine - An integrated database for Drosophila genomics

FBgn0032297

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17124

MARRVEL_MODEL - MARRVEL (model organism gene)

34485

Linkouts

BioGRID - A database of protein and genetic interactions.

60559

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032297

DroID - A comprehensive database of gene and protein interactions.

FBgn0032297

DRSC - Results frm RNAi screens

FBgn0032297

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG17124_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032297

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032297

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17124

Flygut - An atlas of the Drosophila adult midgut

FBgn0032297

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032297

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012424

MIST (protein-protein) - An integrated Molecular Interaction Database

34485

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

RHO GTPases activate PKNs

References (50)

The following external sites may use different assemblies or annotations than FlyBase.

-0.46

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