FB2020_06, released Dec 22, 2020

Gene: Dmel\dgt2

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General Information
Symbol
Dmel\dgt2
Species
D. melanogaster
Name
dim γ-tubulin 2
Annotation Symbol
CG16969
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032390
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Key Links
Genomic Location
Cytogenetic map
33B5-33B5
Sequence location
2L:11,989,936..11,990,979 [+]
Recombination map

2-45

RefSeq locus
NT_033779 REGION:11989936..11990979
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables microtubule binding
inferred from direct assay
(Meireles et al., 2009)
enables protein binding
inferred from physical interaction with FLYBASE:wac; FB:FBgn0035120
(Meireles et al., 2009)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in meiotic spindle
inferred from direct assay
(Colombié et al., 2013)
part_of protein-containing complex
inferred from direct assay
(Meireles et al., 2009)
inferred from genetic interaction with FLYBASE:wac; FB:FBgn0035120
(Meireles et al., 2009)
located_in spindle
inferred from direct assay
(Meireles et al., 2009, Goshima et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    As part of the augmin complex, plays a role in centrosome-independent generation of spindle microtubules (PubMed:18443220). The complex is required for mitotic spindle assembly through its involvement in localizing gamma-tubulin to spindle microtubules (PubMed:17412918). dgt2 binds to microtubules in vitro (PubMed:19289792).
    (UniProt, Q9VKD6)
    Gene Model and Products
    Number of Transcripts
    2
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\dgt2 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.51

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    dgt2-RA
    FBtr0080285
    NM_135687
    842
    231
    dgt2-RB
    FBtr0342580
    NM_001298927
    987
    231
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    dgt2-PA
    FBpp0079869
    25.8
    231
    6.27
    NP_609531
    AAF53140
    dgt2-PB
    FBpp0309535
    25.8
    231
    6.27
    NP_001285856
    AHN54370
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    231 aa isoforms: dgt2-PA, dgt2-PB
    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Component of the augmin complex composed of dgt2, dgt3, dgt4, dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19289792). The complex interacts directly or indirectly with microtubules and is required for centrosome-independent generation of spindle microtubules (PubMed:18443220). dgt2 interacts directly with wac (via coiled coil) (PubMed:19289792).

    (UniProt, Q9VKD6)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dgt2 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 3
    organism

    Comment: maternally deposited

    (Fisher et al., 2012)
    embryonic stage 7 -- 8
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 9 -- 10
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 11 -- 12
    organism | ubiquitous
    (Fisher et al., 2012)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in meiotic spindle
    inferred from direct assay
    (Colombié et al., 2013)
    part_of protein-containing complex
    inferred from direct assay
    (Meireles et al., 2009)
    inferred from genetic interaction with FLYBASE:wac; FB:FBgn0035120
    (Meireles et al., 2009)
    located_in spindle
    inferred from direct assay
    (Meireles et al., 2009, Goshima et al., 2007)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\dgt2 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyExpress - Embryonic expression images (BDGP data)
    • Stages(s) 1-3
    • Stages(s) 7-8
    • Stages(s) 9-10
    • Stages(s) 11-12
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 1 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 5 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of dgt2
    Transgenic constructs containing regulatory region of dgt2
    Deletions and Duplications ( 1 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FNE )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila simulans
    Dsim\GD23803
    Drosophila sechellia
    Dsec\GM26683
    Drosophila erecta
    Dere\GG23751
    Drosophila yakuba
    Dyak\GE18558
    Drosophila ananassae
    Dana\GF15136
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA14243
    Drosophila persimilis
    Dper\GL18538
    Drosophila willistoni
    Dwil\GK15173
    Drosophila virilis
    Dvir\GJ17467
    Drosophila mojavensis
    Dmoj\GI17682
    Drosophila grimshawi
    Dgri\GH13062
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150ANR )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17026942
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY005507
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_14402
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes005285
    Aedes aegypti
    Yellow fever mosquito
    Aaeg\AAEL000476
    Anopheles darlingi
    American malaria mosquito
    Adar\ADAC008860
    Anopheles gambiae
    Malaria mosquito
    Agam\AGAP003225
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ009154
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Ras85D
      enhanceable
      dgt2
      (Friedman et al., 2011)
      External Data
      Subunit Structure (UniProtKB)
      Component of the augmin complex composed of dgt2, dgt3, dgt4, dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19289792). The complex interacts directly or indirectly with microtubules and is required for centrosome-independent generation of spindle microtubules (PubMed:18443220). dgt2 interacts directly with wac (via coiled coil) (PubMed:19289792).
      (UniProt, Q9VKD6 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map

      2-45

      Cytogenetic map
      33B5-33B5
      Sequence location
      2L:11,989,936..11,990,979 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      33B5-33B5
      Limits computationally determined from genome sequence between P{EP}MESK1EP980 and P{PZ}l(2)0181001810&P{EP}CG6785EP340
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-45

      (FlyBase, 2016)

      2-39.0

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (3)
      Genomic Clones (14)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (35)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Meireles et al., 2009, Goshima et al., 2008)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments

        FlyBase curator comment: the insertion in the "d01514" Exelixis line (P{XP}escld01514) was originally assigned to the CG16969 gene in FBrf0179797, resulting in the "CG16969d01514" (FBal0162083) allele. However, FBrf0184340 shows that the insertion is actually within escl.

        (Bloomington Drosophila Stock Center, 2004.11.6)
        Other Comments

        RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

        (Goshima et al., 2007)
        Origin and Etymology
        Discoverer
        Etymology

        Named "dim γ-tubulin 2" for the decreased γ-tubulin staining of the spindle pole following treatment of S2 cells with dgt2.

        (Goshima et al., 2007)
        Identification
        External Crossreferences and Linkouts ( 29 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (4)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (35)