FlyBase Gene Report: Dmel\Ced-12

General Information

Symbol

Dmel\Ced-12

Species

D. melanogaster

Name

Ced-12

Annotation Symbol

CG5336

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032409

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Ced-12 (Ced-12) encodes a protein that functions with Dock family members (encoded by mbc or spg) to regulate GTPase activity. It contributes to myoblast fusion, apoptotic cell engulfment, cell migration, and embryonic cellularization. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

ELMO, dCed-12, engulfment and cell motility

Key Links

Genomic Location

Cytogenetic map

33C4-33C4

Sequence location

2L:12,097,838..12,100,839 [+]

Recombination map

2-46

RefSeq locus

NT_033779 REGION:12097838..12100839

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:msk; FB:FBgn0026252

(Liu et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (11 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from mutant phenotype

(Zhou et al., 2001)

involved_in activation of GTPase activity

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333, FLYBASE:Rac2; FB:FBgn0014011, FLYBASE:mbc; FB:FBgn0015513

(Geisbrecht et al., 2008)

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333, FLYBASE:mbc; FB:FBgn0015513

(Geisbrecht et al., 2008)

involved_in border follicle cell migration

inferred from mutant phenotype

(Geisbrecht et al., 2008, Bianco et al., 2007)

involved_in cell migration

inferred from genetic interaction with WB:WBGene00000426

(Zhou et al., 2001)

involved_in central nervous system development

inferred from mutant phenotype

(Biersmith et al., 2011)

involved_in imaginal disc fusion, thorax closure

inferred from mutant phenotype

(Ishimaru et al., 2004)

involved_in muscle attachment

inferred from mutant phenotype

(Liu et al., 2013)

inferred from genetic interaction with FLYBASE:mbc; FB:FBgn0015513

(Liu et al., 2013)

inferred from genetic interaction with FLYBASE:msk; FB:FBgn0026252

(Liu et al., 2013)

involved_in myoblast fusion

inferred from mutant phenotype

(Kim et al., 2015, Geisbrecht et al., 2008)

involved_in positive regulation of JNK cascade

inferred from direct assay

(Ishimaru et al., 2004)

involved_in vascular endothelial growth factor receptor signaling pathway

inferred from mutant phenotype

(Ishimaru et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000299186

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in cell motility

inferred from biological aspect of ancestor with PANTHER:PTN000299186

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Liu et al., 2013)

located_in nucleus

inferred from direct assay

(Liu et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000299186

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Pathway (FlyBase)

Pvr Signaling Pathway Core Components

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

Pvr Signaling Pathway Core Components -: PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ced-12 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKB2)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ced-12-RA

FBtr0080330

NM_135704

2586

724

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ced-12-PA

FBpp0079912

83.0

724

6.10

NP_609548

AAF53164

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ced-12 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

(Geisbrecht et al., 2008)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

somatic mesoderm

(Geisbrecht et al., 2008)

somatic muscle cell

(Geisbrecht et al., 2008)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

visceral mesoderm

(Geisbrecht et al., 2008)

embryonic/larval visceral muscle cell

(Geisbrecht et al., 2008)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Geisbrecht et al., 2008)

adult stage | male

(Geisbrecht et al., 2008)

adult stage | female

(Geisbrecht et al., 2008)

ovary

(Geisbrecht et al., 2008)

Additional Descriptive Data

Ced-12 expression is ubiquitous in embryos in early stages. It is apparent in somatic and visceral mesoderm by stage 11. By stage 13, it is stronger in gut visceral mesoderm than somatic mesoderm. Expression is highest in the visceral mesoderm at stage 16

(Geisbrecht et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- late third instar larval stage

muscle attachment site

(Liu et al., 2013)

larval stage

embryonic/larval hypodermal muscle cell

• Z disc

(Liu et al., 2013)

oogenesis

female germline cell

(Geisbrecht et al., 2008)

oogenesis stage S10

follicle cell

(Geisbrecht et al., 2008)

border follicle cell

(Geisbrecht et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Ced-12 protein is found in close proximity to msk protein at muscle attachment sites.

(Liu et al., 2013)

Broad Ced-12 expression is detected in the ovary throughout oogenesis. It is detected in the germline throughout oogenesis and is detected in follicle cells after stage 9, with prominent expression in the border cells at stage 10. A broad temporal and spatial pattern is seen at other stages of development.

(Geisbrecht et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Liu et al., 2013)

located_in nucleus

inferred from direct assay

(Liu et al., 2013)

Expression Deduced from Reporters

Reporter: PBac{602.P.SVS-1}Ced-12^CPTI000286

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ced-12 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ced-12

View exonic expression by tissue for Dmel\Ced-12

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032409

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34633/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032409

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032409

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032409

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5336

Flygut - An atlas of the Drosophila adult midgut

FBgn0032409

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ced-12

Transgenic constructs containing regulatory region of Ced-12

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)Exel6032

(Ryder, 2004.4.26)

Df(2L)HO55

(Geisbrecht et al., 2008)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Ced-12^c06760

lethal (with Ced-12^8C6)

Ced-12^c06760

lethal (with Ced-12^9F4)

Ced-12^c06760

lethal (with Ced-12^19F3)

Ced-12^c06760

lethal (with Ced-12^c06760)

lethal - all die during embryonic stage | germline clone

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ced-12^GD4186

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ced-12^GD4186

viable, with Scer\GAL4^pnr-MD237

Ced-12^GD4186

viable, with Scer\GAL4^tin.CΔ4

Ced-12^GD4186

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4^alrm.PD

Ced-12^KK102788

abnormal neuroanatomy | embryonic stage

Ced-12^19F3

abnormal neuroanatomy | embryonic stage | germline clone

Ced-12^c06760

some die during embryonic stage | germline clone

Phenotype manifest in

Allele

astrocyte-like glial cell, with Scer\GAL4^alrm.PD

Ced-12^KK102788

border follicle cell, with Scer\GAL4^slbo.2.6

Ced-12^dsRNA.UAS

border follicle cell | somatic clone

egg chamber, with Scer\GAL4^GR1

Ced-12^HM05042

embryonic myoblast

embryonic myoblast (with Df(2L)HO55)

embryonic myoblast | embryonic stage 16, with Scer\GAL4^Mef2.PR

Ced-12^dsRNA.UAS

embryonic myoblast | germline clone (with Df(2L)Prl)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

Ced-12^KK102788

glial cell, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Ced-12^GD4186

lateral longitudinal fascicle

Ced-12^c06760

muscle attachment site | embryonic stage

Ced-12^19F3

myoblast

Ced-12⁵⁰

nurse cell, with Scer\GAL4^GR1

Ced-12^HM05042

presumptive embryonic/larval muscle system

presumptive embryonic/larval muscle system (with Df(2L)HO55)

presumptive embryonic/larval muscle system | embryonic stage 16, with Scer\GAL4^Mef2.PR

Ced-12^dsRNA.UAS

presumptive embryonic/larval muscle system | germline clone (with Df(2L)Prl)

tendon cell | embryonic stage

Ced-12^19F3

thorax, with Scer\GAL4^pnr-MD237

Ced-12^dsRNA.UAS

vesicle, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Ced-12^GD4186

visceral muscle fiber | embryonic stage (with Ced-12^19F3)

Ced-12^KO

visceral muscle fiber | embryonic stage (with Ced-12^KO)

Ced-12^19F3

visceral muscle fiber | embryonic stage | germline clone

Ced-12^c06760

visceral muscle fiber | third instar larval stage, with Scer\GAL4^Mef2.PR

Ced-12^EDE.UAS.EYFP

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ELMO1

13 of 15

Yes

Hsap\ELMO2

11 of 15

Yes

Hsap\ELMO3

10 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Elmo1

13 of 15

Yes

Mmus\Elmo2

10 of 15

Yes

Mmus\Elmo3

10 of 15

Yes

Rattus norvegicus (Norway rat) (4)

Rnor\Elmo3

10 of 13

Yes

Rnor\Elmo1

9 of 13

Yes

Rnor\Elmo2

7 of 13

Yes

Rnor\LOC102555457

6 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\elmo2

7 of 12

Yes

Xtro\elmo3

6 of 12

Yes

Xtro\elmo1

3 of 12

Yes

Danio rerio (Zebrafish) (4)

Drer\elmo1

11 of 15

Yes

Drer\elmo3

11 of 15

Yes

Drer\elmo2

8 of 15

Yes

Drer\si:dkey-56f14.7

5 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ced-12

11 of 15

Yes

Cele\C56G7.3

1 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\AT1G03620

3 of 9

Yes

Atha\AT3G03610

3 of 9

Yes

Atha\AT2G44770

2 of 9

Atha\AT3G43400

2 of 9

Yes

Atha\AT3G60260

2 of 9

Atha\AT1G67400

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\LMO1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ced-12^GD4186

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ELMO1; engulfment and cell motility 1

13 of 15

606420

ELMO2; engulfment and cell motility 2

11 of 15

606421

VASCULAR MALFORMATION, PRIMARY INTRAOSSEOUS; VMPI

ELMO3; engulfment and cell motility 3

10 of 15

606422

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Ced-12 - mir-8

luminiscence technology

(Hyun et al., 2009)

protein-protein

Physical Interaction

Assay

References

Ced-12 - mbc

anti tag coimmunoprecipitation, western blot, two hybrid, anti tag western blot, Identification by mass spectrometry

(Geisbrecht et al., 2008, Ishimaru et al., 2004)

Ced-12 - msk

anti bait coimmunoprecipitation, western blot, proximity ligation assay, tag visualisation, pull down, anti tag western blot

(Liu et al., 2013)

Ced-12 - spg

anti tag coimmunoprecipitation, multidimensional protein identification technology, western blot

(Biersmith et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ced-12

enhanceable

Eato

(Santoso et al., 2018)

Ced-12

enhanceable

mbc

(Geisbrecht et al., 2008)

enhanceable

(Liu et al., 2013)

suppressible

(Liu et al., 2013, Geisbrecht et al., 2008)

enhanceable

(Kim et al., 2015)

suppressible

(Lu et al., 2014)

enhanceable

(Biersmith et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

drpr

enhanceable

Ced-12

(Tasdemir-Yilmaz and Freeman, 2014)

mbc

enhanceable

Ced-12

(Geisbrecht et al., 2008)

suppressible

(Liu et al., 2013)

suppressible

(Liu et al., 2013)

enhanceable

(Liu et al., 2013)

suppressible

(Geisbrecht et al., 2008)

spg

enhanceable

Ced-12

(Biersmith et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60684

DroID - A comprehensive database of gene and protein interactions.

FBgn0032409

MIST (genetic) - An integrated Molecular Interaction Database

Ced-12

MIST (protein-protein) - An integrated Molecular Interaction Database

Ced-12

Pathways

Signaling Pathways (FlyBase)

Pvr Signaling Pathway Core Components -: PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032409

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-46

Cytogenetic map

33C4-33C4

Sequence location

2L:12,097,838..12,100,839 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33C4-33C4

Limits computationally determined from genome sequence between P{EP}JhI-21^EP1187 and P{lacW}Aats-thr^k04203

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-46

(FlyBase, 2016)

2-39.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28556

y¹ v¹; P{TRiP.HM05042}attP2

58153

y¹ v¹; P{TRiP.HMJ22104}attP40

17781

w¹¹¹⁸; PBac{PB}Ced-12^c06760/CyO

36097

y¹ sc^* v¹ sev²¹; P{TRiP.GL00516}attP40

79366

y¹ w^*; TI{CRIMIC.TG4.1}Ced-12^{CR00938-TG4.1}/SM6a

Kyoto

115020

w¹¹¹⁸; PBac{602.P.SVS-1}Ced-12^CPTI000286

VDRC

v107590

P{KK102788}VIE-260B

v10455

w¹¹¹⁸; P{GD4186}v10455

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE62284

Other clones

FI01538

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032409

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032409

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34633

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Geisbrecht et al., 2008)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ced-12 CG5336

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of Ced-12 CG5336 was sequence comparison ( date:020807 ).

(FlyBase, 1996-)

Other Comments

Ced-12 is essential in early, posterior, border cell migration in developing egg chambers, but is not required for later, dorsal, border cell migration.

(Bianco et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34633

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKB2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKB2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032409

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032409

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34633/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032409

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032409

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032409

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5336

Flygut - An atlas of the Drosophila adult midgut

FBgn0032409

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032409

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34633

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002742

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5336

MARRVEL_MODEL - MARRVEL (model organism gene)

34633

modMine - A data warehouse for the modENCODE project

FBgn0032409

Linkouts

BioGRID - A database of protein and genetic interactions.

60684

DroID - A comprehensive database of gene and protein interactions.

FBgn0032409

DRSC - Results frm RNAi screens

FBgn0032409

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032409

FlyMine - An integrated database for Drosophila genomics

FBgn0032409

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Ced-12

MIST (protein-protein) - An integrated Molecular Interaction Database

Ced-12

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG5336

(Kumar et al., 2016, Hosono et al., 2015, Walls et al., 2013, Abmayr and Pavlath, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Geisbrecht et al., 2008, Hughes et al., 2008, Stuart et al., 2007, Ishimaru et al., 2004, Zhou et al., 2001)

Ced-12

(Lebo and McCall, 2021, Hilu-Dadia and Kurant, 2020, La Marca and Richardson, 2020, Gene Disruption Project members, 2018-, Santoso et al., 2018, Tsai et al., 2018, Kutscher and Shaham, 2017, Solis et al., 2017, Winfree et al., 2017, Meehan et al., 2016, Nguyen et al., 2016, Maartens and Brown, 2015, Tasdemir-Yilmaz and Freeman, 2014, Toret et al., 2014, Japanese National Institute of Genetics, 2012.5.21, McPhee et al., 2010, Hyun et al., 2009, Bianco et al., 2007, Stuart et al., 2007)

Ced12

(Wood and Martin, 2017, Hyun et al., 2009)

Dced-12

(Reif and Cyster, 2002, Zhou et al., 2001)

ELMO

(Lv et al., 2021, Dubey et al., 2020, Lv et al., 2020, Enomoto et al., 2018, Richardson and Portela, 2018, Schmidt and Grosshans, 2018, Schmidt et al., 2018, Winkler et al., 2015, Levayer and Moreno, 2013, Pastor-Pareja and Xu, 2013, Sopko and Perrimon, 2013, Tran et al., 2013, Miyamoto and Yamauchi, 2010, Meller et al., 2005, Ishimaru et al., 2004, Ishimaru et al., 2004)

Elmo

(Kanda and Igaki, 2020, Bothe and Baylies, 2016, Biersmith et al., 2015, Abmayr and Pavlath, 2012, McPhee et al., 2010)

ced-12

(Xu et al., 2020, Kelleher et al., 2018, Toret et al., 2018, Etchegaray et al., 2016, Winkler et al., 2015, Geisbrecht et al., 2007, Zhou et al., 2001)

ced12

(Blanchard et al., 2014)

dCED-12

(Davidson and Wood, 2020)

dCed-12

(Freeman, 2015, Lu et al., 2014, Ziegenfuss et al., 2012)

dCed12

(Pearce et al., 2015)

dced-12

(GOETHEM and FRANC, 2007)

dced12

(Nakano et al., 2019)

elmo

(Lee and Chen, 2019, Toret et al., 2018, Kim et al., 2015, Shiratsuchi et al., 2012, Biersmith et al., 2011, Ohsawa et al., 2011, Kuraishi et al., 2009, Geisbrecht et al., 2008, Huang et al., 2008, Bianco et al., 2007)

Name Synonyms

Ced-12

(McPhee et al., 2010)

Elmo

(Amoyel and Bach, 2014)

engulfment and cell motility

(Levayer and Moreno, 2013, Bianco et al., 2007)

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (110)

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