FlyBase Gene Report: Dmel\Ced-12

General Information

Symbol

Dmel\Ced-12

Species

D. melanogaster

Name

Ced-12

Annotation Symbol

CG5336

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032409

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Snapshot

Ced-12 (Ced-12) encodes a protein that functions with Dock family members (encoded by mbc or spg) to regulate GTPase activity. It contributes to myoblast fusion, apoptotic cell engulfment, cell migration, and embryonic cellularization. [Date last reviewed: 2018-11-15]

Other Summaries

Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

ELMO, dCed-12, engulfment and cell motility

Key Links

Genomic Location

Cytogenetic map

33C4-33C4

Sequence location

2L:12,097,838..12,100,839 [+]

Recombination map

2-46

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Pathway (FlyBase)

Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

protein binding

Predictions / Assertions

Summaries

Pathway (FlyBase)

Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -: PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Ced-12 or the JBrowse view of Dmel\Ced-12 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ced-12-RA

FBtr0080330

NM_135704

2586

724

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ced-12-PA

FBpp0079912

83.0

724

6.10

NP_609548

AAF53164

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ced-12 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein binding

inferred from physical interaction with FLYBASE:msk; FB:FBgn0026252

(Liu et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (10 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

actin filament organization

inferred from mutant phenotype

(Zhou et al., 2001)

activation of GTPase activity

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333, FLYBASE:Rac2; FB:FBgn0014011, FLYBASE:mbc; FB:FBgn0015513

(Geisbrecht et al., 2008)

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333, FLYBASE:mbc; FB:FBgn0015513

(Geisbrecht et al., 2008)

border follicle cell migration

inferred from mutant phenotype

(Geisbrecht et al., 2008, Bianco et al., 2007)

cell migration

inferred from genetic interaction with WB:WBGene00000426

(Zhou et al., 2001)

central nervous system development

inferred from mutant phenotype

(Biersmith et al., 2011)

imaginal disc fusion, thorax closure

inferred from mutant phenotype

(Ishimaru et al., 2004)

muscle attachment

inferred from mutant phenotype

(Liu et al., 2013)

inferred from genetic interaction with FLYBASE:mbc; FB:FBgn0015513

(Liu et al., 2013)

inferred from genetic interaction with FLYBASE:msk; FB:FBgn0026252

(Liu et al., 2013)

myoblast fusion

inferred from mutant phenotype

(Kim et al., 2015, Geisbrecht et al., 2008)

positive regulation of JNK cascade

inferred from direct assay

(Ishimaru et al., 2004)

vascular endothelial growth factor receptor signaling pathway

inferred from mutant phenotype

(Ishimaru et al., 2004)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Liu et al., 2013)

nucleus

inferred from direct assay

(Liu et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

(Geisbrecht et al., 2008)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

somatic mesoderm

(Geisbrecht et al., 2008)

somatic muscle

(Geisbrecht et al., 2008)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

visceral mesoderm

(Geisbrecht et al., 2008)

embryonic/larval visceral muscle

(Geisbrecht et al., 2008)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Geisbrecht et al., 2008)

adult stage | male

(Geisbrecht et al., 2008)

adult stage | female

(Geisbrecht et al., 2008)

ovary

(Geisbrecht et al., 2008)

Additional Descriptive Data

Ced-12 expression is ubiquitous in embryos in early stages. It is apparent in somatic and visceral mesoderm by stage 11. By stage 13, it is stronger in gut visceral mesoderm than somatic mesoderm. Expression is highest in the visceral mesoderm at stage 16

(Geisbrecht et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- late third instar larval stage

muscle attachment site

(Liu et al., 2013)

larval stage

embryonic/larval hypodermal muscle

• Z disc

(Liu et al., 2013)

oogenesis

female germline cell

(Geisbrecht et al., 2008)

oogenesis stage S10

follicle cell

(Geisbrecht et al., 2008)

border follicle cell

(Geisbrecht et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Ced-12 protein is found in close proximity to msk protein at muscle attachment sites.

(Liu et al., 2013)

Broad Ced-12 expression is detected in the ovary throughout oogenesis. It is detected in the germline throughout oogenesis and is detected in follicle cells after stage 9, with prominent expression in the border cells at stage 10. A broad temporal and spatial pattern is seen at other stages of development.

(Geisbrecht et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Liu et al., 2013)

nucleus

inferred from direct assay

(Liu et al., 2013)

Expression Deduced from Reporters

Reporter: PBac{602.P.SVS-1}Ced-12^CPTI000286

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ced-12 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ced-12

View exonic expression by tissue for Dmel\Ced-12

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032409

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0032409

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5336-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5336

Flygut - An atlas of the Drosophila adult midgut

FBgn0032409

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ced-12

Transgenic constructs containing regulatory region of Ced-12

Deletions and Duplications ( 2 )

Disrupted in

Df(2L)Exel6032

(Ryder, 2004.4.26)

Df(2L)HO55

(Geisbrecht et al., 2008)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Ced-12^c06760

lethal (with Ced-12^8C6)

Ced-12^c06760

lethal (with Ced-12^9F4)

Ced-12^c06760

lethal (with Ced-12^19F3)

Ced-12^c06760

lethal (with Ced-12^c06760)

lethal - all die before end of embryonic stage | germline clone

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ced-12^GD4186

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ced-12^GD4186

viable, with Scer\GAL4^pnr-MD237

Ced-12^GD4186

viable, with Scer\GAL4^tin.CΔ4

Ced-12^GD4186

Other Phenotypes

Allele

endocytosis defective, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Ced-12^GD4186

neuroanatomy defective, with Scer\GAL4^alrm.PD

Ced-12^KK102788

neuroanatomy defective | embryonic stage

Ced-12^19F3

neuroanatomy defective | embryonic stage | germline clone

Ced-12^c06760

some die during embryonic stage | germline clone

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^slbo.2.6

Ced-12^dsRNA.UAS

border follicle cell | somatic clone

egg chamber, with Scer\GAL4^GR1

Ced-12^HM05042

embryonic myoblast

embryonic myoblast (with Df(2L)HO55)

embryonic myoblast | embryonic stage 16, with Scer\GAL4^Mef2.PR

Ced-12^dsRNA.UAS

embryonic myoblast | germline clone (with Df(2L)Prl)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

Ced-12^KK102788

glial cell, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Ced-12^GD4186

lateral longitudinal fascicle

Ced-12^c06760

muscle attachment site | embryonic stage

Ced-12^19F3

myoblast

Ced-12⁵⁰

nurse cell, with Scer\GAL4^GR1

Ced-12^HM05042

presumptive embryonic/larval muscle system

presumptive embryonic/larval muscle system (with Df(2L)HO55)

presumptive embryonic/larval muscle system | embryonic stage 16, with Scer\GAL4^Mef2.PR

Ced-12^dsRNA.UAS

presumptive embryonic/larval muscle system | germline clone (with Df(2L)Prl)

reticular neuropil associated glial cell, with Scer\GAL4^alrm.PD

Ced-12^KK102788

tendon cell | embryonic stage

Ced-12^19F3

thorax, with Scer\GAL4^pnr-MD237

Ced-12^dsRNA.UAS

vesicle, with Scer\GAL4^repo, Scer\GAL80^ts.αTub84B

Ced-12^GD4186

visceral muscle fiber | embryonic stage (with Ced-12^19F3)

Ced-12^KO

visceral muscle fiber | embryonic stage (with Ced-12^KO)

Ced-12^19F3

visceral muscle fiber | embryonic stage | germline clone

Ced-12^c06760

visceral muscle fiber | third instar larval stage, with Scer\GAL4^Mef2.PR

Ced-12^EDE.UAS.EYFP

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ELMO1

13 of 15

Yes

Hsap\ELMO2

11 of 15

Yes

Hsap\ELMO3

10 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Elmo1

13 of 15

Yes

Mmus\Elmo2

11 of 15

Yes

Mmus\Elmo3

10 of 15

Yes

Rattus norvegicus (Norway rat) (4)

Rnor\Elmo3

10 of 13

Yes

Rnor\Elmo1

9 of 13

Yes

Rnor\LOC102555457

6 of 13

Yes

Rnor\Elmo2

5 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\elmo1

7 of 12

Yes

Xtro\elmo3

3 of 12

Yes

Xtro\elmo2

2 of 12

Yes

Danio rerio (Zebrafish) (4)

Drer\elmo1

11 of 15

Yes

Drer\elmo3

10 of 15

Yes

Drer\elmo2

9 of 15

Yes

Drer\si:dkey-56f14.7

4 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ced-12

11 of 15

Yes

Cele\C56G7.3

1 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\AT1G03620

3 of 9

Yes

Atha\AT3G03610

3 of 9

Yes

2 of 9

2 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190324 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Ced-12

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18811

Drosophila persimilis

Dper\GL19238

Drosophila willistoni

Dwil\GK21087

Drosophila virilis

Dvir\GJ17651

Drosophila mojavensis

Dmoj\GI17067

Drosophila grimshawi

Dgri\GH11577

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502MS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015123

Glossina morsitans

Tsetse fly

Gmor\GMOY001986

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05838

Mayetiola destructor

Hessian fly

Mdes\Mdes005001

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001223

Anopheles darlingi

American malaria mosquito

Adar\ADAC002044

Anopheles gambiae

Malaria mosquito

Agam\AGAP009236

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004175

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02E8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010524

Danaus plexippus

Monarch butterfly

Dple\DPOGS207663-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002674

Apis florea

Little honeybee

Aflo\2184453812

Apis mellifera

Western honey bee

Amel\Ced-12

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10564

Bombus terrestris

Buff-tailed bumblebee

Bter\15204765

Linepithema humile

Argentine ant

Lhum\LH23522

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0007d4

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010481

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07406

Tribolium castaneum

Red flour beetle

Tcas\TC002742

Pediculus humanus

Human body louse

Phum\PHUM347560

Rhodnius prolixus

Kissing bug

Rpro\RPRC011187

Cimex lectularius

Bed bug

Clec\CLEC006363

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002214

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07231

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02BW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010272

Ixodes scapularis

Black-legged tick

Isca\ISCW009354

Ixodes scapularis

Black-legged tick

Isca\ISCW020886

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72867.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72866.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g08770

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_308856

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03JJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Elmo

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000000597

Gallus gallus

Domestic chicken

Ggal\ELMO3

Gallus gallus

Domestic chicken

Ggal\ELMO1

Gallus gallus

Domestic chicken

Ggal\ELMO2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ELMO1; engulfment and cell motility 1

13 of 15

606420

ELMO2; engulfment and cell motility 2

11 of 15

606421

VASCULAR MALFORMATION, PRIMARY INTRAOSSEOUS

ELMO3; engulfment and cell motility 3

10 of 15

606422

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Ced-12 - mir-8

luminiscence technology

(Hyun et al., 2009)

protein-protein

Physical Interaction

Assay

References

Ced-12 - mbc

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot, two hybrid, anti tag western blot

(Geisbrecht et al., 2008, Ishimaru et al., 2004)

Ced-12 - msk

pull down, western blot, proximity ligation assay, tag visualisation, anti bait coimmunoprecipitation, anti tag western blot

(Liu et al., 2013)

Ced-12 - spg

anti tag coimmunoprecipitation, multidimensional protein identification technology, western blot

(Biersmith et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ced-12

enhanceable

Eato

(Santoso et al., 2018)

Ced-12

enhanceable

mbc

(Geisbrecht et al., 2008)

enhanceable

(Liu et al., 2013)

suppressible

(Liu et al., 2013, Geisbrecht et al., 2008)

enhanceable

(Kim et al., 2015)

suppressible

(Lu et al., 2014)

enhanceable

(Biersmith et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

drpr

enhanceable

Ced-12

(Tasdemir-Yilmaz and Freeman, 2014)

mbc

enhanceable

Ced-12

(Geisbrecht et al., 2008)

enhanceable

(Liu et al., 2013)

suppressible

(Liu et al., 2013)

suppressible

(Liu et al., 2013)

suppressible

(Geisbrecht et al., 2008)

spg

enhanceable

Ced-12

(Biersmith et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60684

DroID - A comprehensive database of gene and protein interactions.

FBgn0032409

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34633

MIST (genetic) - An integrated Molecular Interaction Database

Ced-12

MIST (protein-protein) - An integrated Molecular Interaction Database

Ced-12

Pathways

Gene Group - Pathway Membership (FlyBase)

Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -: PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-46

Cytogenetic map

33C4-33C4

Sequence location

2L:12,097,838..12,100,839 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33C4-33C4

Limits computationally determined from genome sequence between P{EP}JhI-21^EP1187 and P{lacW}Aats-thr^k04203

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-46

(FlyBase, 2016)

2-39.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

28556

y¹ v¹; P{TRiP.HM05042}attP2

58153

y¹ v¹; P{TRiP.HMJ22104}attP40

17781

w¹¹¹⁸; PBac{PB}Ced-12^c06760/CyO

36097

y¹ sc^* v¹ sev²¹; P{TRiP.GL00516}attP40

79366

y¹ w^*; TI{CRIMIC.TG4.1}Ced-12^{CR00938-TG4.1}/SM6a

Harvard

c06760

PBac{PB}Ced-12^c06760

Kyoto

115020

w¹¹¹⁸; PBac{602.P.SVS-1}Ced-12^CPTI000286

VDRC

v107590

P{KK102788}VIE-260B

v10455

w¹¹¹⁸; P{GD4186}v10455

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RE62284

Other clones

FI01538

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032409

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032409

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34633

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Geisbrecht et al., 2008)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ced-12 CG5336

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of Ced-12 CG5336 was sequence comparison ( date:020807 ).

(FlyBase, 1996-)

Other Comments

Ced-12 is essential in early, posterior, border cell migration in developing egg chambers, but is not required for later, dorsal, border cell migration.

(Bianco et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34633

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032409

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032409

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5336

Flygut - An atlas of the Drosophila adult midgut

FBgn0032409

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34633

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002742

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5336

modMine - A data warehouse for the modENCODE project

FBgn0032409

Linkouts

BioGRID - A database of protein and genetic interactions.

60684

DroID - A comprehensive database of gene and protein interactions.

FBgn0032409

DRSC - Results frm RNAi screens

FBgn0032409

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0032409

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5336-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0032409

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34633

MIST (genetic) - An integrated Molecular Interaction Database

Ced-12

MIST (protein-protein) - An integrated Molecular Interaction Database

Ced-12

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG5336

(Kumar et al., 2016, Hosono et al., 2015, Walls et al., 2013, Abmayr and Pavlath, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Geisbrecht et al., 2008, Hughes et al., 2008, Stuart et al., 2007, Ishimaru et al., 2004, Zhou et al., 2001)

Ced-12

(Gene Disruption Project members, 2018-, Santoso et al., 2018, Tsai et al., 2018, Kutscher and Shaham, 2017, Winfree et al., 2017, Meehan et al., 2016, Nguyen et al., 2016, Maartens and Brown, 2015, Tasdemir-Yilmaz and Freeman, 2014, Toret et al., 2014, Japanese National Institute of Genetics, 2012.5.21, McPhee et al., 2010, Hyun et al., 2009, Bianco et al., 2007, Stuart et al., 2007)

Ced12

(Wood and Martin, 2017, Hyun et al., 2009)

Dced-12

(Reif and Cyster, 2002, Zhou et al., 2001)

ELMO

(Enomoto et al., 2018, Richardson and Portela, 2018, Schmidt and Grosshans, 2018, Schmidt et al., 2018, Winkler et al., 2015, Levayer and Moreno, 2013, Pastor-Pareja and Xu, 2013, Sopko and Perrimon, 2013, Tran et al., 2013, Miyamoto and Yamauchi, 2010, Meller et al., 2005, Ishimaru et al., 2004, Ishimaru et al., 2004)

Elmo

(Bothe and Baylies, 2016, Biersmith et al., 2015, Abmayr and Pavlath, 2012, McPhee et al., 2010)

ced-12

(Toret et al., 2018, Winkler et al., 2015, Geisbrecht et al., 2007, Zhou et al., 2001)

ced12

(Blanchard et al., 2014)

dCed-12

(Freeman, 2015, Lu et al., 2014, Ziegenfuss et al., 2012)

dCed12

(Pearce et al., 2015)

dced-12

(GOETHEM and FRANC, 2007)

dced12

(Nakano et al., 2019)

elmo

(Toret et al., 2018, Kim et al., 2015, Shiratsuchi et al., 2012, Biersmith et al., 2011, Ohsawa et al., 2011, Kuraishi et al., 2009, Geisbrecht et al., 2008, Huang et al., 2008, Bianco et al., 2007)

Name Synonyms

Ced-12

(McPhee et al., 2010)

Elmo

(Amoyel and Bach, 2014)

engulfment and cell motility

(Levayer and Moreno, 2013, Bianco et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (97)

Report Sections

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