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Gene Dmel\Vha68-3

General Information
Symbol	Dmel\Vha68-3	Species	D. melanogaster
Name	Vacuolar H+ ATPase 68kD subunit 3	Annotation symbol	CG5075
Feature type	protein_coding_gene	FlyBase ID	FBgn0032464
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	vha68-3
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	34A3-34A3	Sequence location	2L:12,978,786..12,981,293 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vacuolar H+ ATPase 68kD subunit 3 is referred to in FlyBase by the symbol Dmel\Vha68-3 (CG5075, FBgn0032464). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: ATP binding; proton-transporting ATPase activity, rotational mechanism. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: ATP hydrolysis coupled proton transport; ATP metabolic process. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, F1 complex beta subunit/V1 complex, C-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain; ATPase, V1 complex, subunit A; ATPase, alpha/beta subunit, N-terminal; ATPase, alpha/beta subunit, nucleotide-binding domain, active site; V-type ATP synthase catalytic subunit A/alpha. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: larval fat body. Comments on Affy2 ProbeSet: ProbeSet 1628908_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG5075. Gene sequence location is 2L:12978786..12981293.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34A3-34A3   Limits computationally determined from genome sequence between P{lacW}l(2)k07015k07015&P{PZ}l(2)rK639rK639 and P{lacW}l(2)k11328k11328&P{lacW}l(2)k10105k10105
Experimentally Determined Cytological Location
Cytogenetic map Notes References 34B-34B     (Dow, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vha68-3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vha68-3-RA FBtr0080421  NM_135751   2445   743
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vha68-3-PA   FBpp0080002   82.3   743   4.85     NP_609595   AAF53234
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit A (IPR005725) P-loop containing nucleoside triphosphate hydrolase (IPR027417) ATPase, F1 complex beta subunit/V1 complex, C-terminal (IPR024034) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase catalytic subunit A/alpha (IPR022878)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF53234   AI114011   AI387094   AI945166   AI945385   AI946075   AY089332 AAL90070   BF486355   BF486417   BF486729   BF487046   BF487393   BF487835   BF489384   BF489538   BF490145   BF491184   BF491440   BF493317   BF493729   BF494094   BF494315   BF494491   BF494858   BF495022   BF495537   BF495760   BF495781   BF496314   BF496495   BF496981   BF497518   BF497751   BF497785   BF497796   BF497797   BF497900   BF497909   BF499325   BF499369   BF500002   BF500532   BF500563   BF501184   BF502352   BF503527   BF504012   BF504974   BF505504   BF505575   BF505749   BF505880   BF506216   BF506709   BF506715   BG631916   BG633751   BG634793   BG635391   BT031085   CK130600
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VK47 Q8T464
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006, Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: larval fat body. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis    1403.1 Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis  (1403.1) Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis  (1403.1) Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis    1403.1 Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis    1403.1 Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis  (1403.1) Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis  1403.1 Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.525 Larval Midgut    6.3 Larval Hindgut    1.5 Larval Malpighian Tubules    1.3 Larval Fat Body    145.5 Larval Salivary Gland    4.2 Larval Trachea    3.75 Larval Carcass    2.675 Adult Head    0.7 Adult Eye    0.9 Adult Brain    1.2 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    5.5 Adult Midgut    2.9 Adult Hindgut    3.8 Adult Malpighian Tubules    3.4 Adult Fat Body    0.6 Adult Salivary Gland    10 Adult Heart    2.7 Adult VirginFemale Spermatheca    3.6 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    1 Adult Testis    1403.1 Adult Male Accessory Gland    3.3 Adult Carcass    11.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    0.7   NA  Eye    0.9   NA  Brain    1.2   2.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.9   NA  Crop    5.5   6.3  Midgut    2.9   1.5  Hindgut    3.8   1.3  Malpighian Tubules    3.4   145.5  Fat Body    0.6   4.2  Salivary Gland    no informative data   NA  Heart    2.7   3.75  Trachea    NA   NA  VirginFemale Spermatheca    3.6   NA  InseminatedFemale Spermatheca    3.4   NA  Ovary    1   NA  Testis    1403.1   NA  Male Accessory Gland    3.3   2.675  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    375 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (46) central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (375) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (375) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    375 accessory gland, mated 4-day male    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    375 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (46) central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (375) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (375) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    10 fat body, pupae P8    15 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    375 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day    102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr  (33) pupae 2d postWPP  (43) pupae 3d postWPP  (48) pupae 4d postWPP  (50) adult male 01day  (73) adult male 05day  (94) adult male 30day  (102) adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day  102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day    102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day    102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr  (33) pupae 2d postWPP  (43) pupae 3d postWPP  (48) pupae 4d postWPP  (50) adult male 01day  (73) adult male 05day  (94) adult male 30day  (102) adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day  102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    22 white prepupae 24hr    33 pupae 2d postWPP    43 pupae 3d postWPP    48 pupae 4d postWPP    50 adult male 01day    73 adult male 05day    94 adult male 30day    102 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_33 mE1_20_mRNA_expression_cluster_18
External Data & Images
Linkouts	BDGP expression data • AT09773 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032464 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5075-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Vha68-3GD11527 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Vha68-3 Class Mutagen Stocks Known lesion Vha68-3MI03173 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vha68-3 Class Mutagen Stocks Known lesion Vha68-3GD11527 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes Vha68-3HMS02647 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vha68-3NIG.5075R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7055 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.5075R} NA P{TRiP.HMS02647} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Vha68-3MI03173 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000194, InterPro:IPR020003 (FlyBase Curators et al., 2004-) proton-transporting ATPase activity, rotational mechanism inferred from electronic annotation with InterPro:IPR004100, InterPro:IPR005725, InterPro:IPR018118 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ATP hydrolysis coupled proton transport inferred from electronic annotation with InterPro:IPR000793, InterPro:IPR005725 (FlyBase Curators et al., 2004-) ATP metabolic process inferred from electronic annotation with InterPro:IPR004100, InterPro:IPR018118, InterPro:IPR022878 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
vacuolar proton-transporting V-type ATPase, V1 domain inferred from sequence or structural similarity with UniProtKB:P31400 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vha68-3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60735 DroID - A comprehensive database of gene and protein interactions. • FBgn0032464 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34695
Orthologs
OrthoDB Orthologs (24) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VX2QV Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search EOG6CRJF3 Metazoa inclusive ortholog search EOG6FFRTT
Orthologs in Drosophila Species (EOG6VX2QV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Vha68-3     Drosophila simulans   Dsim\GD23861     Drosophila sechellia   Dsec\GM10088     Drosophila erecta   Dere\GG23805 Y   Drosophila yakuba   Dyak\GE18611     Drosophila ananassae   Dana\GF14215     Drosophila pseudoobscura pseudoobscura   Dpse\GA18641 Y   Drosophila persimilis   Dper\GL24733     Drosophila willistoni   Dwil\GK15309 Y   Drosophila virilis   Dvir\GJ16815 Y   Drosophila mojavensis   Dmoj\GI18089     Drosophila grimshawi   Dgri\GH11386
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (EOG6CRJF3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Eif3s6ip Y Ixodes scapularis Deer tick  Isca\ISCW002559 Y
Orthologs in non-Arthropod Metazoa (EOG6FFRTT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\vha-13 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016882gn Y Danio rerio Zebrafish  Drer\ENSDARG00000076318 Y Danio rerio Zebrafish  Drer\atp6v1al Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000000901 Y Xenopus tropicalis Western clawed frog  Xtro\atp6v1a Y Gallus gallus Domestic chicken  Ggal\ATP6V1A Y Mus musculus House mouse  Mmus\Atp6v1a Y Rattus norvegicus Norway rat  Rnor\Atp6v1a Y Homo sapiens Human  Hsap\ATP6V1A Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\ATP6V1A
AAA Orthologs (4) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila erecta Dere\GG23805 Drosophila pseudoobscura pseudoobscura Dpse\GA18641 Drosophila willistoni Dwil\GK15309 Drosophila virilis Dvir\GJ16815
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	36424 y1 w*; Mi{MIC}Vha68-3MI03173/SM6a 42954 y1 sc* v1; P{TRiP.HMS02647}attP40
VDRC	v34926 w1118; P{GD11527}v34926 v34927 w1118; P{GD11527}v34927 v41646 w1118; P{GD11527}v41646
Genomic Clones ( 2 )
	BACR11M13 BACR48D10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 57 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT09773 AT13860
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01645 AT02063 AT02516 AT02765 AT03622 AT03832 AT04480 AT04722 AT05273 AT07128 AT07741 AT07828 AT08037 AT08118 AT08633 AT09264 AT09271 AT09413 AT09436 AT10079 AT10431 AT11015 AT11704 AT12027 AT12077 AT12089 AT12090 AT12127 AT12213 AT12230 AT13915 AT14789 AT15511 AT15546 AT16438 AT18052 AT20630 AT20693 AT21239 AT21718 AT22178 AT22894 AT25388 AT25586 AT26442 AT26707 AT27867 AT28180 AT29474 AT31136 AT31965
Other clones	AT03123 bs09g02 bs12c08 bs20e12
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0032464 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34695
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Vha68-3 CG5075 (Allan et al., 2005)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135751 RefSeq (Proteins) • NP_609595 DDBJ / EMBL / GenBank • AAF53234 AAL90070 AI114011 AI387094 AI945166 AI945385 AI946075 AY089332 BF499325 Entrez Gene - A searchable database of RefSeq genes. • 34695 UniProtKB/TrEMBL • Q8T464 Q9VK47
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit A (IPR005725) P-loop containing nucleoside triphosphate hydrolase (IPR027417) ATPase, F1 complex beta subunit/V1 complex, C-terminal (IPR024034) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase catalytic subunit A/alpha (IPR022878) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • AT09773
Linkouts
	BioGRID - A database of protein and genetic interactions • 60735 DroID - A comprehensive database of gene and protein interactions. • FBgn0032464 DRSC - Results from RNAi screens. • FBgn0032464 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032464 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5075-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0032464 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34695 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34695 modMine - Data generated by the modENCODE project. • FBgn0032464
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG5075 (Wang et al., 2004, Allan et al., 2005, Dorus et al., 2006, Wasbrough et al., 2010, Seong et al., 2011, Doggett et al., 2011, Ni et al., 2010.12.1, Japanese National Institute of Genetics, 2012.5.21) vha67-3 (Dow, 1999) Vha68 (Guo et al., 1996) vha68-3 (Wang et al., 2004, Allan et al., 2005) Vha68-3
Name Synonym	Vacuolar H+ ATPase 68kD subunit 3   Vacuolar H+ ATPase subunit 68-3
Secondary FlyBase IDs
References ( 25 )
Generate a list of	All references relating to this gene ( 25 )
List References by type	Research paper  ( 6 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Doggett et al., 2011, Dev. Biol. 355(2): 381--393 Wake-up-call, a lin-52 paralogue, and Always early, a lin-9 homologue physically interact, but have opposing functions in regulating testis-specific gene expression. [FBrf0213964] Seong et al., 2011, Biogerontology 12(2): 93--107 Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity. [FBrf0213307] Show all research papers ...