FlyBase Gene Report: Dmel\loqs

General Information

Symbol

Dmel\loqs

Species

D. melanogaster

Name

loquacious

Annotation Symbol

CG6866

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032515

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

loquacious (loqs) encodes a double-stranded RNA binding protein with multiple splicing isoforms. loqs-PB and loqs-PA associate with the product of Dcr-1 to facilitate processing of precursor miRNA into mature miRNA, whereas loqs-PD interacts with the product of Dcr-2 to enhance processing of dsRNA into siRNA. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

R3D1, TRBP, R3D1-L, R3D1-S, Loqs-PD

Key Links

Genomic Location

Cytogenetic map

34B9-34B9

Sequence location

2L:13,382,652..13,385,671 [+]

Recombination map

2-48

RefSeq locus

NT_033779 REGION:13382652..13385671

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016

(Ye et al., 2007)

enables RISC complex binding

inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016

(Fukunaga et al., 2012)

enables siRNA binding

inferred from direct assay

(Tants et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables double-stranded RNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (10 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in central nervous system development

inferred from mutant phenotype

(Bennett et al., 2008)

involved_in dosage compensation by hyperactivation of X chromosome

inferred from genetic interaction with FLYBASE:lncRNA:roX2; FB:FBgn0019660, FLYBASE:lncRNA:roX1; FB:FBgn0019661

(Menon and Meller, 2012)

involved_in female germ-line stem cell asymmetric division

inferred from mutant phenotype

(Park et al., 2007)

involved_in germ-line stem cell population maintenance

inferred from mutant phenotype

(Park et al., 2007)

involved_in miRNA metabolic process

inferred from direct assay

(Marques et al., 2010)

involved_in pre-miRNA processing

inferred from mutant phenotype

(Fukunaga et al., 2012, Hartig et al., 2009, Zhou et al., 2009, Forstemann et al., 2005, Jiang et al., 2005, Saito et al., 2005)

inferred from direct assay

(Ye et al., 2007)

involved_in primary sncRNA processing

inferred from direct assay

(Marques et al., 2010)

involved_in production of siRNA involved in RNA interference

inferred from mutant phenotype

(Hartig et al., 2009, Zhou et al., 2009, Okamura et al., 2008)

inferred from direct assay

(Marques et al., 2010)

involved_in regulation of production of small RNA involved in gene silencing by RNA

inferred from direct assay

(Cenik et al., 2011)

involved_in small RNA loading onto RISC

inferred from mutant phenotype

(Tants et al., 2017)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Saito et al., 2005)

part_of RISC-loading complex

inferred from physical interaction with FLYBASE:loqs; FB:FBgn0032515

(Tants et al., 2017)

inferred from physical interaction with FLYBASE:loqs; FB:FBgn0032515, FLYBASE:Dcr-1; FB:FBgn0039016

(Miyoshi et al., 2009)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

miRNA RISC LOADING COMPLEX

ALTERNATIVE siRNA RISC-LOADING COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

miRNA RISC LOADING COMPLEX -: The miRNA-RISC loading complex binds microRNA (miRNA) and loads it onto AGO1 to form an RNA-induced silencing complex (RISC). (Adapted from FBrf0215456.)
ALTERNATIVE siRNA RISC-LOADING COMPLEX -: The alternative siRNA-RISC loading complex (aRLC) has been characterised in S2 cells. In the aRLC, loqs replaces r2d2 as the double-stranded RNA-binding domain-containing protein. siRNA RISC-loading complex binds small interfering RNAs (siRNAs) siRNA and loads it onto AGO2 to form RNA-induced silencing complexes (RISCs). (Adapted from FBrf0237457.)

Summary (Interactive Fly)

a component of a functional pre-miRNA processing complex - stimulates and directs pre-microRNA processing activity - a Dicer-1 partner that enhances miRNA processing from hairpins with unstable structures at the dicing site

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\loqs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VJY9)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

loqs-RA

FBtr0080497

NM_165041

1879

419

loqs-RB

FBtr0080498

NM_135802

2017

465

loqs-RC

FBtr0100220

NM_001038814

2147

383

loqs-RD

FBtr0303269

NM_001201867

1334

359

loqs-RE

FBtr0342632

NM_001298964

1876

418

loqs-RF

FBtr0342633

NM_001298965

2014

464

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

loqs-PA

FBpp0080075

44.9

419

8.36

NP_723813

AAF53296

loqs-PB

FBpp0080076

50.1

465

9.25

NP_609646

AAF53295

loqs-PC

FBpp0099590

41.1

383

8.80

NP_001033903

ABC65897

loqs-PD

FBpp0292361

38.5

359

8.11

NP_001188796

ADV37046

loqs-PE

FBpp0309561

44.7

418

8.14

NP_001285893

AHN54407

loqs-PF

FBpp0309562

50.0

464

9.10

NP_001285894

AHN54408

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\loqs using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Saito et al., 2005)

part_of RISC-loading complex

inferred from physical interaction with FLYBASE:loqs; FB:FBgn0032515

(Tants et al., 2017)

inferred from physical interaction with FLYBASE:loqs; FB:FBgn0032515, FLYBASE:Dcr-1; FB:FBgn0039016

(Miyoshi et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\loqs in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\loqs

View exonic expression by tissue for Dmel\loqs

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032515

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34751/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032515

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032515

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032515

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6866

Flygut - An atlas of the Drosophila adult midgut

FBgn0032515

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\loqs

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of loqs

Transgenic constructs containing regulatory region of loqs

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)loqs^del

(Ghildiyal et al., 2006)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | embryonic stage | recessive | rescuable maternal effect

loqs^KO

lethal | pharate adult stage | recessive

loqs^KO

lethal | recessive

loqs^f00791

lethal - all die during embryonic stage

loqs^KO

lethal - all die during embryonic stage, with loqs^KO

loqs^PD.Tag:FLAG

lethal - all die during embryonic stage, with loqs^PD.Tag:FLAG

loqs^KO

lethal - all die during embryonic stage | paternal effect

loqs^f00791

viable

loqs^f00791

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

loqs^GD12060

viable, with loqs^KO

viable, with loqs^PA.Tag:FLAG

loqs^KO

viable, with loqs^PB.Tag:FLAG

loqs^KO

viable, with Scer\GAL4^Mef2.PR

loqs^GD12060

viable, with Scer\GAL4^pnr-MD237

loqs^GD12060

viable, with Scer\GAL4^tin.CΔ4

loqs^GD12060

Sterility

Allele

female sterile

loqs^f00791

female sterile, with loqs^KO

loqs^PA.Tag:FLAG

female sterile, with loqs^KO, loqs^PA.Tag:FLAG

loqs^PD.Tag:FLAG

female sterile, with loqs^KO, loqs^PD.Tag:FLAG

loqs^PA.Tag:FLAG

female sterile, with loqs^PA.Tag:FLAG

loqs^KO

female sterile, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

loqs^KO

female sterile | recessive

loqs^f00791

fertile, with loqs^KO

loqs^PB.Tag:FLAG

fertile, with loqs^PB.Tag:FLAG

loqs^KO

male fertile, with loqs^KO

loqs^PA.Tag:FLAG

male fertile, with loqs^PA.Tag:FLAG

loqs^KO

male semi-sterile

loqs^f00791

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage | recessive

loqs^f00791

abnormal neuroanatomy | conditional

loqs^f00791

abnormal neuroanatomy | embryonic stage

loqs^f00791

non-suppressor of variegation

loqs^KO

short lived

loqs^f00791

some die during embryonic stage

loqs^KO

some die during embryonic stage, with loqs^KO

loqs^PD.Tag:FLAG

some die during embryonic stage, with loqs^PD.Tag:FLAG

loqs^KO

some die during embryonic stage | paternal effect

loqs^f00791

Phenotype manifest in

Allele

dorsal appendage, with loqs^KO

loqs^PA.Tag:FLAG

dorsal appendage, with loqs^KO, loqs^PA.Tag:FLAG

loqs^PD.Tag:FLAG

dorsal appendage, with loqs^KO, loqs^PD.Tag:FLAG

loqs^PA.Tag:FLAG

dorsal appendage, with loqs^PA.Tag:FLAG

loqs^KO

dorsal appendage, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

loqs^KO

egg chamber

loqs^f00791

eye

loqs^f00791

female germline stem cell

loqs^f00791

female germline stem cell (with loqs^f00791)

loqs^KO

female germline stem cell (with loqs^KO)

loqs^f00791

female germline stem cell | germline clone

loqs^KO

follicle cell

loqs^f00791

germarium

loqs^f00791

germline stem cell, with loqs^KO, loqs^PA.Tag:FLAG

loqs^PD.Tag:FLAG

germline stem cell, with loqs^KO, loqs^PD.Tag:FLAG

loqs^PA.Tag:FLAG

germline stem cell, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

loqs^KO

lamina | conditional

loqs^f00791

larval longitudinal connective

loqs^f00791

larval ventral nerve cord commissure

loqs^f00791

ovariole

loqs^f00791

ovary

loqs^f00791

retina | conditional

loqs^f00791

spectrosome

loqs^f00791

testis

loqs^f00791

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PRKRA

13 of 15

Yes

Hsap\TARBP2

12 of 15

Yes

3 of 15

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Prkra

12 of 15

Yes

Mmus\Tarbp2

12 of 15

Yes

3 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Prkra

12 of 13

Yes

Rnor\Tarbp2

10 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\tarbp2

11 of 12

Yes

Xtro\prkra

3 of 12

Yes

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (14)

Drer\prkra

12 of 15

Yes

Drer\tarbp2

12 of 15

Yes

3 of 15

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\stau-1

2 of 15

Yes

Cele\rde-4

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\DRB4

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

3 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PRKRA; protein activator of interferon induced protein kinase EIF2AK2

model of dystonia 16

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

loqs^f00791

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Bilen et al., 2006)

modeled by Hsap\MAPT^UAS.cWa

(Bilen et al., 2006)

modeled by Hsap\MAPT^R406W.UAS

(Bilen et al., 2006)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRKRA; protein activator of interferon induced protein kinase EIF2AK2

13 of 15

603424

DYSTONIA 16; DYT16

dystonia 16

TARBP2; TARBP2 subunit of RISC loading complex

12 of 15

605053

STAU1; staufen double-stranded RNA binding protein 1

3 of 15

601716

STAU2; staufen double-stranded RNA binding protein 2

3 of 15

605920

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

loqs

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, molecular sieving

(Jakob et al., 2016, Hartig and Förstemann, 2011, Zhou et al., 2009)

loqs - AGO1

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Miyoshi et al., 2009, Jiang et al., 2005, Saito et al., 2005)

loqs - Bacc

experimental knowledge based

(Guruharsha et al., 2011)

loqs - CG1910

experimental knowledge based

(Guruharsha et al., 2011)

loqs - CG6726

experimental knowledge based

(Guruharsha et al., 2011)

loqs - Chd64

experimental knowledge based

(Guruharsha et al., 2011)

loqs - Df31

experimental knowledge based

(Guruharsha et al., 2011)

loqs - Gip

experimental knowledge based

(Guruharsha et al., 2011)

loqs - Sxl

pull down, peptide massfingerprinting

(Graindorge et al., 2013)

loqs - capt

experimental knowledge based

(Guruharsha et al., 2011)

loqs - eEF2

experimental knowledge based

(Guruharsha et al., 2011)

loqs - r2d2

anti tag coimmunoprecipitation, western blot

(Miyoshi et al., 2010)

loqs - scu

experimental knowledge based

(Guruharsha et al., 2011)

loqs - stau

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

RNA-protein

Physical Interaction

Assay

References

loqs - mir-8

fluorescence polarization spectroscopy, predetermined participant

(Tants et al., 2017)

loqs - mir-ban

fluorescence polarization spectroscopy, predetermined participant, anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation

(Tants et al., 2017, Miyoshi et al., 2009, Saito et al., 2005)

loqs - msl-2

pull down, peptide massfingerprinting

(Graindorge et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

lncRNA:roX1

enhanceable

loqs

(Menon and Meller, 2012)

suppressible

(Pek et al., 2009)

suppressible

(Neumuller et al., 2008)

suppressible

loqs

(Fukunaga et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60786

DroID - A comprehensive database of gene and protein interactions.

FBgn0032515

MIST (protein-protein) - An integrated Molecular Interaction Database

loqs

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032515

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-48

Cytogenetic map

34B9-34B9

Sequence location

2L:13,382,652..13,385,671 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34B9-34B9

Limits computationally determined from genome sequence between P{lacW}l(2)k00302^k00302 and P{PZ}kuz⁰³⁷⁸²&P{lacW}kuz^k14817

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-48

(FlyBase, 2016)

2-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28963

y¹ v¹; P{TRiP.HM05174}attP2

65266

w^*; P{loqs-PA.myc}3/TM3, Sb¹

18371

w¹¹¹⁸; PBac{WH}loqs^f00791/CyO

65411

w^*; TI{TI}loqs^KO P{neoFRT}40A/CyO

63371

w¹¹¹⁸; PBac{IT.GAL4}loqs^0512-G4/CyO

32955

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00749}attP2

33427

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00311}attP2

34779

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00088}attP2

34780

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00089}attP2

34781

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00090}attP2

VDRC

v108358

P{KK100016}VIE-260B

v22453

w¹¹¹⁸ P{GD12060}v22453

More stocks available...

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (79)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE14437

Other clones

DPiM_RE14437

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032515

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032515

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34751

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016, Miyoshi et al., 2009)

polyclonal

(Hartig and Förstemann, 2011, Jiang et al., 2005)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: loqs CG6866

(Forstemann et al., 2005.4.25)

Source for database merge of

Additional comments

Other Comments

loqs acts primarily to process exogenous dsRNA.

(Marques et al., 2010)

Production of endogenous siRNAs depends on the loqs-PD isoform.

(Hartig et al., 2009)

The loqs-PD isoform is capable of associating with the Dcr-2 protein, but not with the Dcr-1 protein. The loqs-PB and loqs-PA isoforms can associate with the Dcr-1 protein, and also to some extent with Dcr-2 protein.

(Hartig et al., 2009)

Production of endogenous siRNAs depends on the loqs-PD isoform, whereas production of mature microRNAs from the pre-miRNA precursor requires the loqs-PB isoform.

(Zhou et al., 2009)

The loqs-PB isoform, and to a lesser extent the loqs-PA isoform, is capable of associating with the Dcr-1 protein. The loqs-PD isoform is capable of associating with the Dcr-2 protein, but not with the Dcr-1 protein.

(Zhou et al., 2009)

Production of mature siRNAs from endogenous long hairpin RNA genes is a hybrid mechanism that combines canonical RNA interference factors (Dcr-2, Hen1 and AGO2) and a canonical microRNA factor loqs.

(Okamura et al., 2008)

loqs is required intrinsically for germline stem cell maintenance in the ovary.

(Park et al., 2007)

loqs is not required for repeat-associated small interfering RNA (rasiRNA) production

(Vagin et al., 2006)

loqs is required together with Dcr-1 for normal pre-miRNA processing. loqs is required for the normal silencing of the endogenous Ste locus in the testis and appears to be required for maintenance of the germ-line stem cells in the ovary.

(Forstemann et al., 2005)

loqs protein alone is not capable of converting pre-miRNAs into mature miRNAs. However, the loqs and Dcr-1 proteins form a complex that converts pre-miRNAs into mature miRNAs, and the loqs protein stimulates and directs the specific pre-miRNA processing activity of Dcr-1 protein.

(Saito et al., 2005)

Origin and Etymology

Discoverer

Etymology

The gene is named "loquacious" because loss of function de-silences both endogenous silencing and reporter expression in vivo.

(Forstemann et al., 2005)

Gene is designated as "loquacious" ("very talkative") as several types of silencing are lost in mutant flies.

(Saito et al., 2005)

Identification

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34751

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VJY9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VJY9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032515

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032515

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34751/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032515

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032515

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032515

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6866

Flygut - An atlas of the Drosophila adult midgut

FBgn0032515

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032515

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34751

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011666

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6866

MARRVEL_MODEL - MARRVEL (model organism gene)

34751

modMine - A data warehouse for the modENCODE project

FBgn0032515

Linkouts

BioGRID - A database of protein and genetic interactions.

60786

DroID - A comprehensive database of gene and protein interactions.

FBgn0032515

DRSC - Results frm RNAi screens

FBgn0032515

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032515

FlyMine - An integrated database for Drosophila genomics

FBgn0032515

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

loqs

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG6866

(Çiçek et al., 2016, Na et al., 2016, Graindorge et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Bennett et al., 2008, Jiang et al., 2005, Xu et al., 2004)

LOQS

(Kolaczkowski et al., 2011, Miyoshi et al., 2009)

Loqs

(Naganuma et al., 2021, Rosendo Machado et al., 2021, Bansal et al., 2020, Donelick et al., 2020, Fareh et al., 2018, Heyam et al., 2017, Kennedy et al., 2017, Tants et al., 2017, Trettin et al., 2017, Azlan et al., 2016, Jakob et al., 2016, Yang et al., 2016, Singh, 2015, Sinha et al., 2015, Bogerd et al., 2014, Yang et al., 2014, Gomes et al., 2013, Graindorge et al., 2013, Nishida et al., 2013, Wu et al., 2013, Xiong et al., 2013, Cenik et al., 2011, Han et al., 2011, Hartig and Förstemann, 2011, Ni et al., 2011, Park et al., 2011, Tsutsumi et al., 2011, Marques et al., 2010, Miyoshi et al., 2010, Chung et al., 2008, Okamura et al., 2008, Liu et al., 2007, Nurminsky, 2007, Ye et al., 2007, Brodersen and Voinnet, 2006, Du et al., 2006, Meyer et al., 2006, Vagin et al., 2006, Filipowicz, 2005)

Loqs-PD

(Mérel et al., 2020, Sinha and Bass, 2017)

LqPD

(Fareh et al., 2018)

R3D1

(Bennett et al., 2008, Bilen et al., 2006, Obbard et al., 2006, Obbard et al., 2006.2.10, Jiang et al., 2005)

R3D1-L

(Jiang et al., 2005, Liu and Du, 2005.7.6)

R3D1-S

(Jiang et al., 2005, Liu, 2005.7.6)

TRBP

(Meister, 2013, Brodersen and Voinnet, 2006, Carthew, 2006)

cg6866

(Lasko, 2000)

dRax

(Bennett et al., 2008)

loq

(Pek et al., 2009)

loqs

(Lécureuil et al., 2021, Nitschko et al., 2020, Drummond-Barbosa, 2019, Eyk et al., 2019, Fingerhut et al., 2019, Luhur et al., 2019, Xie et al., 2019, Clandinin and Owens, 2016-, Funikov et al., 2016, Na et al., 2016, Chak et al., 2015, Khan et al., 2015, Wen et al., 2015, Mirkovic-Hösle and Förstemann, 2014, Olesnicky et al., 2014, Okamura et al., 2013, Fukunaga et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Liu et al., 2012, Menon and Meller, 2012, Dufourt et al., 2011, Han et al., 2011, Kim and Orr-Weaver, 2011, Lindquist et al., 2011, Liu et al., 2011, Okamura et al., 2011, Khurana et al., 2010, Czech et al., 2009, Fagegaltier et al., 2009, Graze et al., 2009, Hartig et al., 2009, Jepson and Reenan, 2009, Martin et al., 2009, Okamura et al., 2009, Pek et al., 2009, Reich et al., 2009, Zhou et al., 2009, Bennett et al., 2008, Czech et al., 2008, Huang et al., 2008, Neumuller et al., 2008, Okamura et al., 2008, Förstemann et al., 2007, Josse et al., 2007, Okamura et al., 2007, Park et al., 2007, Park et al., 2007, Park et al., 2007, Yang et al., 2007, Vagin et al., 2006, Vagin et al., 2006)

loquacious

(Pool et al., 2012, Bilen et al., 2006)

r3d1

(Park et al., 2006)

Name Synonyms

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (168)

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