FB2020_01, released Feb 12, 2020

Gene: Dmel\loqs

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General Information
Symbol
Dmel\loqs
Species
D. melanogaster
Name
loquacious
Annotation Symbol
CG6866
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032515
Gene Model Status
Current
Stock Availability
15 publicly available
Gene Snapshot
loquacious (loqs) encodes a double-stranded RNA binding protein with multiple splicing isoforms. loqs-PB and loqs-PA associate with the product of Dcr-1 to facilitate processing of precursor miRNA into mature miRNA, whereas loqs-PD interacts with the product of Dcr-2 to enhance processing of dsRNA into siRNA. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

R3D1, TRBP, R3D1-L, R3D1-S

Key Links
Genomic Location
Cytogenetic map
34B9-34B9
Sequence location
2L:13,382,652..13,385,671 [+]
Recombination map

2-48

RefSeq locus
NT_033779 REGION:13382652..13385671
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
protein binding; RISC complex binding
Predictions / Assertions
double-stranded RNA binding
Summaries
Summary (Interactive Fly)

a component of a functional pre-miRNA processing complex - stimulates and directs pre-microRNA processing activity - a Dicer-1 partner that enhances miRNA processing from hairpins with unstable structures at the dicing site

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\loqs or the JBrowse view of Dmel\loqs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
loqs-RA
FBtr0080497
NM_165041
1879
419
loqs-RB
FBtr0080498
NM_135802
2017
465
loqs-RC
FBtr0100220
NM_001038814
2147
383
loqs-RD
FBtr0303269
NM_001201867
1334
359
loqs-RE
FBtr0342632
NM_001298964
1876
418
loqs-RF
FBtr0342633
NM_001298965
2014
464
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
loqs-PA
FBpp0080075
44.9
419
8.36
NP_723813
AAF53296
loqs-PB
FBpp0080076
50.1
465
9.25
NP_609646
AAF53295
loqs-PC
FBpp0099590
41.1
383
8.80
NP_001033903
ABC65897
loqs-PD
FBpp0292361
38.5
359
8.11
NP_001188796
ADV37046
loqs-PE
FBpp0309561
44.7
418
8.14
NP_001285893
AHN54407
loqs-PF
FBpp0309562
50.0
464
9.10
NP_001285894
AHN54408
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\loqs using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (13 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
protein binding
inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016
(Ye et al., 2007)
RISC complex binding
inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016
(Fukunaga et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
double-stranded RNA binding
inferred from sequence or structural similarity
(Lasko, 2000)
Biological Process (9 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Saito et al., 2005)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Saito et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\loqs in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of loqs
Transgenic constructs containing regulatory region of loqs
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | embryonic stage | recessive | rescuable maternal effect
lethal | pharate adult stage | recessive
lethal | recessive
lethal - all die before end of embryonic stage
lethal - all die before end of embryonic stage, with loqsKO
lethal - all die before end of embryonic stage, with loqsPD.Tag:FLAG
lethal - all die before end of embryonic stage | paternal effect
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with loqsKO
viable, with loqsPA.Tag:FLAG
viable, with loqsPB.Tag:FLAG
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile
female sterile, with loqsKO
female sterile, with loqsKO, loqsPA.Tag:FLAG
female sterile, with loqsKO, loqsPD.Tag:FLAG
female sterile, with loqsPA.Tag:FLAG
female sterile, with loqsPA.Tag:FLAG, loqsPD.Tag:FLAG
female sterile | recessive
fertile, with loqsKO
fertile, with loqsPB.Tag:FLAG
male fertile, with loqsKO
male fertile, with loqsPA.Tag:FLAG
male semi-sterile
Other Phenotypes
Allele
locomotor behavior defective | adult stage | recessive
neuroanatomy defective | conditional
neuroanatomy defective | embryonic stage
non-suppressor of variegation
short lived
some die during embryonic stage
some die during embryonic stage, with loqsKO
some die during embryonic stage, with loqsPD.Tag:FLAG
some die during embryonic stage | paternal effect
Phenotype manifest in
Allele
dorsal appendage, with loqsKO
dorsal appendage, with loqsKO, loqsPA.Tag:FLAG
dorsal appendage, with loqsKO, loqsPD.Tag:FLAG
dorsal appendage, with loqsPA.Tag:FLAG
dorsal appendage, with loqsPA.Tag:FLAG, loqsPD.Tag:FLAG
egg chamber
eye
female germline stem cell
female germline stem cell (with loqsf00791)
female germline stem cell (with loqsKO)
female germline stem cell | germline clone
follicle cell
germarium
germline stem cell, with loqsKO, loqsPA.Tag:FLAG
germline stem cell, with loqsKO, loqsPD.Tag:FLAG
germline stem cell, with loqsPA.Tag:FLAG, loqsPD.Tag:FLAG
lamina | conditional
longitudinal connective
ovariole
ovary
retina | conditional
spectrosome
testis
ventral nerve cord commissure
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PRKRA
13 of 15
Yes
Yes
Hsap\TARBP2
12 of 15
No
Yes
Hsap\STAU1
2 of 15
No
No
Hsap\STAU2
2 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Prkra
12 of 15
Yes
Yes
Mmus\Tarbp2
12 of 15
Yes
Yes
Mmus\Stau1
1 of 15
No
No
Mmus\Stau2
1 of 15
No
No
Rattus norvegicus (Norway rat) (4)
Rnor\Prkra
11 of 13
Yes
Yes
Rnor\Tarbp2
10 of 13
No
Yes
Rnor\Stau1
1 of 13
No
No
Rnor\Stau2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\tarbp2
9 of 12
Yes
Yes
Xtro\prkra
2 of 12
No
Yes
Xtro\stau2
2 of 12
No
No
Xtro\stau1
1 of 12
No
No
Danio rerio (Zebrafish) (4)
Drer\prkra
11 of 15
Yes
Yes
Drer\tarbp2
11 of 15
Yes
Yes
Drer\stau1
2 of 15
No
No
Drer\stau2
2 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\stau-1
2 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
Atha\RTL1
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091909WX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\loqs
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001672
Drosophila simulans
Dsim\loqs
Drosophila sechellia
Dsec\GM10477
Drosophila erecta
Dere\GG23848
Drosophila yakuba
Dyak\GE18652
Drosophila ananassae
Dana\GF23153
Drosophila pseudoobscura pseudoobscura
Dpse\GA19915
Drosophila pseudoobscura pseudoobscura
Dpse\GA22530
Drosophila persimilis
Dper\GL26052
Drosophila willistoni
Dwil\GK14845
Drosophila virilis
Dvir\GJ17975
Drosophila mojavensis
Dmoj\GI17218
Drosophila grimshawi
Dgri\GH13466
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BGY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011564
Glossina morsitans
Tsetse fly
Gmor\GMOY008059
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11780
Mayetiola destructor
Hessian fly
Mdes\Mdes008816
Aedes aegypti
Yellow fever mosquito
Aaeg\loqs
Anopheles darlingi
American malaria mosquito
Adar\ADAC006775
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004832
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0DCT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA005395
Danaus plexippus
Monarch butterfly
Dple\DPOGS209755-PA
Heliconius melpomene
Postman butterfly
Hmel\R2D2-Loq
Apis mellifera
Western honey bee
Amel\LOC552226
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17206
Linepithema humile
Argentine ant
Lhum\LH26150
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002dc9
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010715
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10909
Tribolium castaneum
Red flour beetle
Tcas\TC011666
Pediculus humanus
Human body louse
Phum\PHUM559590
Rhodnius prolixus
Kissing bug
Rpro\loqs
Cimex lectularius
Bed bug
Clec\CLEC012943
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI072122
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10745
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BF6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR009997
Ixodes scapularis
Black-legged tick
Isca\ISCW023181
Stegodyphus mimosarum
African social velvet spider
Smim\KFM70247.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g00430
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g00410
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_24493
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0I2L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_007500gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Tarbp2L
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000011306
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
r2d2
2 of 10
stau
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PRKRA; protein activator of interferon induced protein kinase EIF2AK2
    13 of 15
    603424
    TARBP2; TARBP2 subunit of RISC loading complex
    12 of 15
    605053
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        loqs
        anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, molecular sieving
        (Jakob et al., 2016, Hartig and Förstemann, 2011, Zhou et al., 2009)
        loqs - AGO1
        anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
        (Miyoshi et al., 2009, Jiang et al., 2005, Saito et al., 2005)
        loqs - Bacc
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - CG1910
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - CG6726
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - Chd64
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - Dcr-1
        anti tag coimmunoprecipitation, anti tag western blot, fluorescence correlation spectroscopy, anti bait coimmunoprecipitation, western blot, electrophoretic mobility shift assay, autoradiography, ion exchange chromatography, pull down, Identification by mass spectrometry, cosedimentation, peptide massfingerprinting
        (Jakob et al., 2016, Miyoshi et al., 2010, Hartig et al., 2009, Miyoshi et al., 2009, Zhou et al., 2009, Czech et al., 2008, Ye et al., 2007, Forstemann et al., 2005, Jiang et al., 2005, Saito et al., 2005)
        loqs - Dcr-2
        anti tag coimmunoprecipitation, western blot, molecular sieving, cross-linking study, molecular weight estimation by staining, anti tag western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, peptide massfingerprinting, pull down
        (Kennedy et al., 2017, Sinha and Bass, 2017, Tants et al., 2017, Trettin et al., 2017, Hartig and Förstemann, 2011, Miyoshi et al., 2010, Hartig et al., 2009, Zhou et al., 2009, Czech et al., 2008)
        loqs - Df31
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - Gip
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - Sxl
        pull down, peptide massfingerprinting
        (Graindorge et al., 2013)
        loqs - capt
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - eEF2
        experimental knowledge based
        (Guruharsha et al., 2011)
        loqs - r2d2
        anti tag coimmunoprecipitation, western blot
        (Miyoshi et al., 2010)
        loqs - scu
        experimental knowledge based
        (Guruharsha et al., 2011)
        RNA-protein
        Physical Interaction
        Assay
        References
        loqs - mir-8
        fluorescence polarization spectroscopy, predetermined participant
        (Tants et al., 2017)
        loqs - mir-ban
        fluorescence polarization spectroscopy, predetermined participant, anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation
        (Tants et al., 2017, Miyoshi et al., 2009, Saito et al., 2005)
        loqs - msl-2
        pull down, peptide massfingerprinting
        (Graindorge et al., 2013)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        lncRNA:roX1
        enhanceable
        loqs
        (Menon and Meller, 2012)
        mael
        suppressible
        loqs
        (Pek et al., 2009)
        mei-P26
        suppressible
        loqs
        (Neumuller et al., 2008)
        w
        suppressible
        loqs
        (Fukunaga et al., 2012)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-48

        Cytogenetic map
        34B9-34B9
        Sequence location
        2L:13,382,652..13,385,671 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        34B9-34B9
        Limits computationally determined from genome sequence between P{lacW}l(2)k00302k00302 and P{PZ}kuz03782&P{lacW}kuzk14817
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-48

        (FlyBase, 2016)

        2-43.8

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (15)
        Genomic Clones (10)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (79)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (Na et al., 2016, Miyoshi et al., 2009)

        polyclonal

        (Hartig and Förstemann, 2011, Jiang et al., 2005)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: loqs CG6866

        (Forstemann et al., 2005.4.25)
        Source for database merge of
        Additional comments
        Other Comments

        loqs acts primarily to process exogenous dsRNA.

        (Marques et al., 2010)

        Production of endogenous siRNAs depends on the loqs-PD isoform.

        (Hartig et al., 2009)

        The loqs-PD isoform is capable of associating with the Dcr-2 protein, but not with the Dcr-1 protein. The loqs-PB and loqs-PA isoforms can associate with the Dcr-1 protein, and also to some extent with Dcr-2 protein.

        (Hartig et al., 2009)

        Production of endogenous siRNAs depends on the loqs-PD isoform, whereas production of mature microRNAs from the pre-miRNA precursor requires the loqs-PB isoform.

        (Zhou et al., 2009)

        The loqs-PB isoform, and to a lesser extent the loqs-PA isoform, is capable of associating with the Dcr-1 protein. The loqs-PD isoform is capable of associating with the Dcr-2 protein, but not with the Dcr-1 protein.

        (Zhou et al., 2009)

        Production of mature siRNAs from endogenous long hairpin RNA genes is a hybrid mechanism that combines canonical RNA interference factors (Dcr-2, Hen1 and AGO2) and a canonical microRNA factor loqs.

        (Okamura et al., 2008)

        loqs is required intrinsically for germline stem cell maintenance in the ovary.

        (Park et al., 2007)

        loqs is not required for repeat-associated small interfering RNA (rasiRNA) production

        (Vagin et al., 2006)

        loqs is required together with Dcr-1 for normal pre-miRNA processing. loqs is required for the normal silencing of the endogenous Ste locus in the testis and appears to be required for maintenance of the germ-line stem cells in the ovary.

        (Forstemann et al., 2005)

        loqs protein alone is not capable of converting pre-miRNAs into mature miRNAs. However, the loqs and Dcr-1 proteins form a complex that converts pre-miRNAs into mature miRNAs, and the loqs protein stimulates and directs the specific pre-miRNA processing activity of Dcr-1 protein.

        (Saito et al., 2005)
        Origin and Etymology
        Discoverer
        Etymology

        The gene is named "loquacious" because loss of function de-silences both endogenous silencing and reporter expression in vivo.

        (Forstemann et al., 2005)

        Gene is designated as "loquacious" ("very talkative") as several types of silencing are lost in mutant flies.

        (Saito et al., 2005)
        Identification
        External Crossreferences and Linkouts ( 79 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (16)
        Reported As
        Symbol Synonym
        CG6866
        (Çiçek et al., 2016, Na et al., 2016, Graindorge et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Bennett et al., 2008, Jiang et al., 2005, Xu et al., 2004)
        LOQS
        (Kolaczkowski et al., 2011, Miyoshi et al., 2009)
        Loqs
        (Fareh et al., 2018, Heyam et al., 2017, Kennedy et al., 2017, Tants et al., 2017, Trettin et al., 2017, Azlan et al., 2016, Jakob et al., 2016, Yang et al., 2016, Singh, 2015, Sinha et al., 2015, Bogerd et al., 2014, Yang et al., 2014, Gomes et al., 2013, Graindorge et al., 2013, Nishida et al., 2013, Wu et al., 2013, Xiong et al., 2013, Cenik et al., 2011, Han et al., 2011, Hartig and Förstemann, 2011, Ni et al., 2011, Park et al., 2011, Tsutsumi et al., 2011, Marques et al., 2010, Miyoshi et al., 2010, Chung et al., 2008, Okamura et al., 2008, Liu et al., 2007, Nurminsky, 2007, Ye et al., 2007, Brodersen and Voinnet, 2006, Du et al., 2006, Meyer et al., 2006, Vagin et al., 2006, Filipowicz, 2005)
        Loqs-PD
        (Sinha and Bass, 2017)
        LqPD
        (Fareh et al., 2018)
        R3D1
        (Bennett et al., 2008, Bilen et al., 2006, Obbard et al., 2006, Obbard et al., 2006.2.10, Jiang et al., 2005)
        R3D1-L
        (Jiang et al., 2005, Liu and Du, 2005.7.6)
        R3D1-S
        (Jiang et al., 2005, Liu, 2005.7.6)
        TRBP
        (Meister, 2013, Brodersen and Voinnet, 2006, Carthew, 2006)
        cg6866
        (Lasko, 2000)
        dRax
        (Bennett et al., 2008)
        loq
        (Pek et al., 2009)
        loqs
        (Drummond-Barbosa, 2019, Fingerhut et al., 2019, Luhur et al., 2019, Clandinin and Owens, 2016-, Funikov et al., 2016, Na et al., 2016, Chak et al., 2015, Khan et al., 2015, Wen et al., 2015, Mirkovic-Hösle and Förstemann, 2014, Olesnicky et al., 2014, Okamura et al., 2013, Fukunaga et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Liu et al., 2012, Menon and Meller, 2012, Dufourt et al., 2011, Han et al., 2011, Kim and Orr-Weaver, 2011, Lindquist et al., 2011, Liu et al., 2011, Okamura et al., 2011, Khurana et al., 2010, Czech et al., 2009, Fagegaltier et al., 2009, Graze et al., 2009, Hartig et al., 2009, Jepson and Reenan, 2009, Martin et al., 2009, Okamura et al., 2009, Pek et al., 2009, Reich et al., 2009, Zhou et al., 2009, Bennett et al., 2008, Czech et al., 2008, Huang et al., 2008, Neumuller et al., 2008, Okamura et al., 2008, Förstemann et al., 2007, Josse et al., 2007, Okamura et al., 2007, Park et al., 2007, Park et al., 2007, Park et al., 2007, Yang et al., 2007, Vagin et al., 2006, Vagin et al., 2006)
        loquacious
        (Pool et al., 2012, Bilen et al., 2006)
        r3d1
        (Park et al., 2006)
        Name Synonyms
        Loquacious
        (Azlan et al., 2016, Jakob et al., 2016, Yang et al., 2016, Cenik et al., 2011, Han et al., 2011, Hartig and Förstemann, 2011, Park et al., 2011, Tsutsumi et al., 2011, Gerbasi et al., 2010, Marques et al., 2010, Miyoshi et al., 2010, Fagegaltier et al., 2009, Reich et al., 2009, Zhou et al., 2009, Okamura et al., 2008, Eulalio et al., 2007, Liu et al., 2007, Park et al., 2007, Yang et al., 2007)
        loquacious
        (Fukunaga et al., 2012, Liu et al., 2012, Han et al., 2011, Lindquist et al., 2011, Liu et al., 2011, Graze et al., 2009, Hartig et al., 2009, Jepson and Reenan, 2009, Pek et al., 2009, Czech et al., 2008, Josse et al., 2007, Park et al., 2007, Ruby et al., 2007, Ruby et al., 2007, Bilen et al., 2006)
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (157)