FB2020_06, released Dec 22, 2020

Gene: Dmel\Hsp60D

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General Information
Symbol
Dmel\Hsp60D
Species
D. melanogaster
Name
Heat shock protein 60D
Annotation Symbol
CG16954
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032525
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Snapshot
Heat shock protein 60D (Hsp60D) encodes a protein involved in caspase-mediated apoptosis and polyQ-mediated neurodegeneration. [Date last reviewed: 2018-10-11]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
34C1-34C1
Sequence location
2L:13,447,538..13,449,497 [-]
Recombination map

2-48

RefSeq locus
NT_033779 REGION:13447538..13449497
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR002423
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATPase activity
inferred from sequence or structural similarity with UniProtKB:P19882
(FlyBase Curators, 2008--)
enables chaperone binding
inferred from biological aspect of ancestor with PANTHER:PTN000143509
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (8 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in apoptotic process
inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997
(Arya and Lakhotia, 2008)
inferred from genetic interaction with FLYBASE:rpr; FB:FBgn0011706
(Arya and Lakhotia, 2008)
inferred from genetic interaction with FLYBASE:grim; FB:FBgn0015946
(Arya and Lakhotia, 2008)
inferred from genetic interaction with FLYBASE:Dronc; FB:FBgn0026404
(Arya and Lakhotia, 2008)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in 'de novo' protein folding
inferred from sequence or structural similarity with UniProtKB:P10809
(Sardiello et al., 2003.6.11)
involved_in apoptotic mitochondrial changes
inferred from biological aspect of ancestor with PANTHER:PTN000143510
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitochondrial unfolded protein response
inferred from biological aspect of ancestor with PANTHER:PTN000143510
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein folding
inferred from biological aspect of ancestor with PANTHER:PTN000143677
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein import into mitochondrial intermembrane space
inferred from biological aspect of ancestor with PANTHER:PTN000143510
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein refolding
inferred from electronic annotation with InterPro:IPR001844
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in protein targeting to mitochondrion
inferred from sequence or structural similarity with UniProtKB:P10809
(Sardiello et al., 2003.6.11)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in mitochondrial inner membrane
inferred from biological aspect of ancestor with PANTHER:PTN000143509
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in mitochondrial matrix
inferred from biological aspect of ancestor with PANTHER:PTN000143509
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:P10809
(Sardiello et al., 2003.6.11)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
HEAT SHOCK PROTEIN 60 CHAPERONINS - GROUP I -
Group I chaperonins catalyse the ATP-dependent folding of proteins. They are found in prokaryotes and endosymbiotic organelles such as mitochondria and chloroplasts. They assemble into large ring-like structures as typified by bacterial GroEL/GroES system. (Adapted from FBrf0190982 and FBrf0232269).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Hsp60D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Hsp60D-RA
FBtr0080517
NM_135812
1894
558
Hsp60D-RB
FBtr0080518
NM_165043
1960
558
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Hsp60D-PA
FBpp0080095
60.3
558
4.93
NP_609656
AAF53308
Hsp60D-PB
FBpp0080096
60.3
558
4.93
NP_723818
AAN10836
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

558 aa isoforms: Hsp60D-PA, Hsp60D-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hsp60D using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data

Hsp60D protein is localized in striated muscles surrounding the egg chamber, and in oviduct muscles; and in follicle cells, nurse cells, and oocytes. Hsp60D protein is specifically localized to the A bands of striated muscles that enwrap the ovariole and individual egg chambers, and the striated muscles of the oviduct. Hsp60D protein localizes to the external face of follicle cells. At oogenesis stage S10-11, Hsp60D protein is present in nurse cell cytoplasm, where it overlaps the distribution of F-actin, particularly near the cell membrane. It also appears to colocalize with the actin skeleton surrounding the nuclei of nurse cells. Less expression is observed in oocytes. After oogenesis stage 13, Hsp60D is largely absent from oocytes, and in nurse cells is concentrated in a small region of the nurse cell-oocyte junction that will form the micropyle of the mature oocyte.

(Sarkar and Lakhotia, 2008)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hsp60D in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hsp60D
Transgenic constructs containing regulatory region of Hsp60D
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\HSPD1
7 of 15
Yes
No
Hsap\BBS10
1 of 15
No
Yes
Hsap\CCT2
1 of 15
No
No
Hsap\CCT3
1 of 15
No
No
Hsap\CCT4
1 of 15
No
No
1  
Hsap\CCT5
1 of 15
No
No
Hsap\CCT6A
1 of 15
No
No
Hsap\CCT6B
1 of 15
No
No
Hsap\CCT7
1 of 15
No
No
Hsap\MKKS
1 of 15
No
Yes
Hsap\TCP1
1 of 15
No
No
Hsap\VKORC1L1
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (11)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Hspd1
6 of 15
Yes
No
Mmus\Bbs10
1 of 15
No
Yes
Mmus\Cct2
1 of 15
No
No
Mmus\Cct3
1 of 15
No
No
Mmus\Cct4
1 of 15
No
No
Mmus\Cct5
1 of 15
No
No
Mmus\Cct6a
1 of 15
No
No
Mmus\Cct6b
1 of 15
No
No
Mmus\Cct7
1 of 15
No
No
Mmus\Mkks
1 of 15
No
Yes
Mmus\Tcp1
1 of 15
No
No
Rattus norvegicus (Norway rat) (11)
Rnor\Hspd1
5 of 13
Yes
No
Rnor\Bbs10
1 of 13
No
Yes
Rnor\Cct2
1 of 13
No
No
Rnor\Cct3
1 of 13
No
No
Rnor\Cct4
1 of 13
No
No
Rnor\Cct5
1 of 13
No
No
Rnor\Cct6a
1 of 13
No
No
Rnor\Cct6b
1 of 13
No
No
Rnor\Cct7
1 of 13
No
No
Rnor\Mkks
1 of 13
No
Yes
Rnor\Tcp1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\hspd1
6 of 12
Yes
No
Xtro\cct2
1 of 12
No
No
Xtro\cct3
1 of 12
No
No
Xtro\cct4
1 of 12
No
No
Xtro\cct5
1 of 12
No
No
Xtro\cct6a
1 of 12
No
No
Xtro\cct7
1 of 12
No
No
Danio rerio (Zebrafish) (10)
Drer\hspd1
6 of 15
Yes
No
Drer\bbs10
1 of 15
No
Yes
Drer\cct2
1 of 15
No
No
Drer\cct3
1 of 15
No
No
Drer\cct4
1 of 15
No
No
Drer\cct5
1 of 15
No
No
Drer\cct6a
1 of 15
No
No
Drer\cct7
1 of 15
No
No
Drer\mkks
1 of 15
No
Yes
Drer\tcp1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\hsp-60
5 of 15
Yes
No
Cele\cct-1
1 of 15
No
No
Cele\cct-2
1 of 15
No
No
Cele\cct-3
1 of 15
No
No
Cele\cct-7
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (10)
Atha\HSP60-2
3 of 9
Yes
No
Atha\HSP60-3A
3 of 9
Yes
No
Atha\HSP60
3 of 9
Yes
No
Atha\CPN60A
2 of 9
No
Yes
Atha\Cpn60alpha2
2 of 9
No
Yes
Atha\CPN60B
2 of 9
No
No
Atha\Cpn60beta2
2 of 9
No
No
Atha\Cpn60beta3
2 of 9
No
No
Atha\Cpn60beta4
2 of 9
No
No
Atha\AT1G26225
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (9)
Scer\HSP60
6 of 15
Yes
No
Scer\TCM62
2 of 15
No
Yes
Scer\CCT2
1 of 15
No
No
Scer\CCT3
1 of 15
No
No
Scer\CCT4
1 of 15
No
No
Scer\CCT5
1 of 15
No
No
Scer\CCT6
1 of 15
No
No
Scer\CCT7
1 of 15
No
No
Scer\TCP1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mcp60
3 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905JY )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001685
Drosophila simulans
Dsim\GD22056
Drosophila sechellia
Dsec\GM25499
Drosophila erecta
Dere\GG10196
Drosophila yakuba
Dyak\GE11569
Drosophila ananassae
Dana\GF21637
Drosophila pseudoobscura pseudoobscura
Dpse\GA14234
Drosophila persimilis
Dper\GL25699
Drosophila willistoni
Dwil\GK15276
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (12)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Hsp60A
5 of 10
Hsp60C
5 of 10
Hsp60B
4 of 10
CCT1
2 of 10
CCT2
2 of 10
CCT3
2 of 10
CCT4
2 of 10
CCT5
2 of 10
CCT6
2 of 10
CCT7
2 of 10
CCT8
1 of 10
fab1
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    HSPD1; heat shock protein family D (Hsp60) member 1
    7 of 15
    118190
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Hsp60D
    enhanceable
    Uba2
    (Arya et al., 2010)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    aos
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    Diap1
    suppressible
    Hsp60D
    (Arya et al., 2010)
    Diap1
    enhanceable
    Hsp60D
    (Arya and Lakhotia, 2008)
    Dronc
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    egr
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    grim
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    hid
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    hid
    enhanceable
    Hsp60D
    (Arya and Lakhotia, 2008)
    rpr
    suppressible
    Hsp60D
    (Arya and Lakhotia, 2008)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-48

    Cytogenetic map
    34C1-34C1
    Sequence location
    2L:13,447,538..13,449,497 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    34C1-34C1
    Limits computationally determined from genome sequence between P{lacW}l(2)k00302k00302 and P{PZ}kuz03782&P{lacW}kuzk14817
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-48

    (FlyBase, 2016)

    2-43.9

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (3)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (38)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Hsp60D CG16954

      (Sarkar and Lakhotia, 2005)
      Source for database merge of
      Additional comments
      Other Comments
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 36 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (genetic) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (4)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (35)