Gene Dmel\CG15160

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\CG15160

Species

D. melanogaster

Name

Annotation symbol

CG15160

Feature type

protein_coding_gene

FlyBase ID

FBgn0032688

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

36F6-36F6

Sequence location

2L:18,590,897..18,595,431 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG15160 (FBgn0032688). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: CID domain; ENTH/VHS; RNA polymerase II-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1635346_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG15160. Gene sequence location is 2L:18590897..18595431.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36F6-36F6

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG15160 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG15160-RA

FBtr0081070

NM_136042

4008

822

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG15160-PA

FBpp0080620

91.0

822

5.32

NP_609886

AAF53677

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

CID domain (IPR006569)

RNA polymerase II-binding domain (IPR006903)

ENTH/VHS (IPR008942)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		173.85
Larval Midgut		21.5
Larval Hindgut		31.6
Larval Malpighian Tubules		28.7
Larval Fat Body		36.7
Larval Salivary Gland		42.8
Larval Trachea		63.225
Larval Carcass		38.8
Adult Head		59.7
Adult Eye		60.525
Adult Brain		132.6
Adult Thoracic-Abdominal Ganglion		121.9
Adult Crop		46.8
Adult Midgut		33.1
Adult Hindgut		35.7
Adult Malpighian Tubules		26.5
Adult Fat Body		37.1
Adult Salivary Gland		66.4
Adult Heart		26.25
Adult VirginFemale Spermatheca		68.4
Adult InseminatedFemale Spermatheca		70.5
Adult Ovary		59.8
Adult Testis		9.9
Adult Male Accessory Gland		64.7
Adult Carcass		24.1
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	59.7
NA	Eye	60.525
NA	Brain	132.6
173.85	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	121.9
NA	Crop	46.8
21.5	Midgut	33.1
31.6	Hindgut	35.7
28.7	Malpighian Tubules	26.5
36.7	Fat Body	37.1
42.8	Salivary Gland	66.4
NA	Heart	26.25
63.225	Trachea	NA
NA	VirginFemale Spermatheca	68.4
NA	InseminatedFemale Spermatheca	70.5
NA	Ovary	59.8
NA	Testis	9.9
NA	Male Accessory Gland	64.7
38.8	Carcass	24.1

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		4
head, mated 1-day female		3
head, mated 4-day female		3
head, mated 20-day female		3
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		2
carcass, larvae L3 wandering		8
carcass, 1-day adult		2
carcass, 4-day adult		3
carcass, 20-day adult		3
ovary, virgin 4-day female		25
ovary, mated 4-day female		20
testis, mated 4-day male		4
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		(29)
embryo 06-08hr		(27)
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		25
embryo 02-04hr		18
embryo 04-06hr		29
embryo 06-08hr		27
embryo 08-10hr		23
embryo 10-12hr		18
embryo 12-14hr		15
embryo 14-16hr		9
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		9
embryo 22-24hr		8
larva L1		6
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		4
pupae 4d postWPP		2
adult male 01day		4
adult male 05day		4
adult male 30day		4
adult female 01day		8
adult female 05day		15
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		11
Schneider line 2 Sg4		12
embryonic 1182-4H		9
embryonic GM2		5
embryonic Kc167		13
embryonic S1		13
embryonic S3		8
leg disc CME L1		13
wing disc CME-W2		10
wing disc ML-DmD8		9
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		11
eye-antennal disc ML-DmD11		11
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		12
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		5
heat shock, 4-day adult		7
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		16
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		13
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		14
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		11
Paraquat 10 mM 48 hrs, 4-day adult		10
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RE28759

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0032688

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG15160-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG15160^GD8932

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG15160	Class	Mutagen	Stocks	Known lesion
CG15160^Roi-1			0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG15160	Mutagen	Stocks	Known lesion
CG15160^GD8932	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG15160^KK100604	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG15160^NIG.15160R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)Exel6041

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD8932}

P{KK100604}

P{NIG.15160R}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG15160 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0032688

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

35109

Orthologs

OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6TB4WV

Dipteran inclusive ortholog search

EOG64F6JD

Insect inclusive ortholog search

EOG64J1F1

Arthropod inclusive ortholog search

EOG6HHMJC

Metazoa inclusive ortholog search

EOG6RRKBF

Orthologs in Drosophila Species (EOG6TB4WV)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13540

Drosophila persimilis

Dper\GL19378

Drosophila willistoni

Dwil\GK24388

Drosophila virilis

Dvir\GJ17766

Drosophila mojavensis

Dmoj\GI14141

Drosophila grimshawi

Dgri\GH11215

Orthologs in non-Drosophila Dipterans (EOG64F6JD)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001651

Anopheles gambiae

Malaria mosquito

Agam\AGAP007819

Orthologs in non-Dipteran Insects (EOG64J1F1)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC408905

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004110

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12281

Atta cephalotes

Leafcutter ant

Acep\ACEP24955

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO15770

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL22980

Linepithema humile

Argentine ant

Lhum\LH21217

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB25822

Solenopsis invicta

Red fire ant

Sinv\SINV22437

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007851

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI067843

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI082219

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI40088

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI31392

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI40087

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI49021

Bombyx mori

Silkmoth

Bmor\BGIBMGA014067

Pediculus humanus

Human body louse

Phum\PHUM388540

Tribolium castaneum

Red flour beetle

Tcas\TC015954

Orthologs in non-Insect Arthropods (EOG6HHMJC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\TCTA

Orthologs in non-Arthropod Metazoa (EOG6RRKBF)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\cids-2

Danio rerio

Zebrafish

Drer\CABZ01081824.1

Danio rerio

Zebrafish

Drer\rprd2

Xenopus tropicalis

Western clawed frog

Xtro\rprd2

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000014656

Mus musculus

House mouse

Mmus\Rprd2

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000013580

Homo sapiens

Human

Hsap\ENSG00000163125

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ENSG00000163125

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13540

Drosophila persimilis

Dper\GL19378

Drosophila willistoni

Dwil\GK24388

Drosophila virilis

Dvir\GJ17766

Drosophila mojavensis

Dmoj\GI14141

Drosophila grimshawi

Dgri\GH11215

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v107028

P{KK100604}VIE-260B

v44600

w¹¹¹⁸; P{GD8932}v44600

v44601

w¹¹¹⁸; P{GD8932}v44601

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 20 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE28759

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0032688

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

35109

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

The "Roi" rough eye mutant allele is due to an inversion between 36A and 37A (In(2L)Roi¹). The proximal breakpoint is 2.6kb downstream of "amos" and is within the last exon of CG15160. The distal breakpoint is within the first intron of CG5888. Several lines of evidence suggest that the "Roi" phenotype is caused by a gain-of-function allele of "amos" rather than being due to an effect of the inversion on another gene; "amos" is expressed in high levels in a broad area surrounding the morphogenetic furrow in "Roi" third instar larvae (in contrast to wild type), overexpression of "amos" in the eye disc can produce a phenotype similar to that of "Roi" and reversion of the phenotype has been achieved by the insertion of a P-element a few kilobases downstream of "amos" which is associated with a severe reduction of "amos" expression in the eye. In addition, although the location of the In(2L)Roi¹ breakpoints means that a chimeric gene could potentially be formed from the 5' portion of CG5888 and the 3' portion of CG15160, this chimeric RNA has not been detected in "Roi" mutants and although dac is also within the inverted segment it does not appear to be implicated in the "Roi" phenotype.

(Chanut et al., 2002)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_136042

RefSeq (Proteins)

•

NP_609886

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

35109

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

CID domain (IPR006569)

RNA polymerase II-binding domain (IPR006903)

ENTH/VHS (IPR008942)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

RE28759

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0032688

DRSC - Results from RNAi screens.

•

FBgn0032688

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0032688

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG15160-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0032688

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

35109

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

35109

modMine - Data generated by the modENCODE project.

•

FBgn0032688

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG15160

(Khokhar et al., 2008, Holohan et al., 2006, Keleman et al., 2009.8.5, Chung et al., 2011, Japanese National Institute of Genetics, 2012.5.21)

Name Synonym

Secondary FlyBase IDs

References ( 14 )

Research paper

Chung et al., 2011, Genome Res. 21(2): 286--300: Computational and experimental identification of mirtrons in Drosophila melanogaster and Caenorhabditis elegans. [FBrf0213087]
Khokhar et al., 2008, Genetics 178(3): 1283--1293: Conditional switches for extracellular matrix patterning in Drosophila melanogaster. [FBrf0205261]
Holohan et al., 2006, BMC Dev. Biol. 6: 53: Multiple enhancers contribute to spatial but not temporal complexity in the expression of the proneural gene, amos. [FBrf0195219]
Chanut et al., 2002, Genetics 160(2): 623--635: Rough eye Is a Gain-of-Function Allele of amos That Disrupts Regulation of the Proneural Gene atonal During Drosophila Retinal Differentiation. [FBrf0144903]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

Stock list

Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.: RNAi stocks available from NIG-FLY. [FBrf0218457]

Website

Fisher et al., 2012, BDGP insitu homepage: BDGP insitu homepage [FBrf0219073]