Gene Dmel\Grip71

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General Information

Symbol

Dmel\Grip71

Species

D. melanogaster

Name

Grip71

Annotation symbol

CG10346

Feature type

protein_coding_gene

FlyBase ID

FBgn0032705

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

37A1-37A1

Sequence location

2L:18,708,849..18,711,305 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Grip71 (CG10346, FBgn0032705). It has the cytological map location 37A1. Its sequence location is 2L:18708849..18711305. Its molecular function is described as gamma-tubulin binding. It is involved in the biological processes: mitosis; regulation of cell cycle; mitotic spindle organization and biogenesis. 4 alleles are reported. The phenotypes of these alleles are annotated with: S2 cell-line; spindle; neuroblast. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37A1-37A1

Limits computationally determined from genome sequence between P{EP}CG10413^EP2164 and P{lacW}l(2)37Db^k16106&P{lacW}Catsup^k05424

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Grip71 for information on other features

Comments on Gene Model

EST data support existence of multiple transcripts due to variable use of 5' exons

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Grip71-RA

FBtr0081099

NM_136058

2281

646

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Grip71-PA

FBpp0080649

71.8

646

7.05

NP_609902

AAF53698

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0032705

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WD40/YVTN repeat-like (IPR015943)

WD40 repeat (IPR001680)

WD40 repeat-like (IPR011046)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

7993

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA949956

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0032705

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0032705

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0032705

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG10346

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Grip71

Stage(s) 1-3

Stage(s) 4-6

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

mitotic cell cycle defective

Sterility

Allele

female sterile

Grip71^GE30807

Phenotype manifest in

Allele

S2 cell-line

Grip71^dsRNA.cVa

spindle

neuroblast

Grip71^GE30807

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Grip71	Class	Mutagen	Stocks	Known lesion
Grip71^EY20619		P-element activity	1	--
Grip71^GE30807			0	Yes

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Grip71	Class	Mutagen	Stocks	Known lesion
Grip71^GD6950		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Grip71^dsRNA.cVa		in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(2L)OD15

Disrupted in

Df(2L)Exel6041

(Ryder, 2004.4.26)

Df(2L)Exel7071

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6950}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}Grip71^EY20619

P{EP}Grip71^GE30807

Related Comments

Please look at the allele reports for the complete phenotype data

SL2 cells treated with dsRNA against Grip71 show a clear increase in the mitotic index compared to control cells.

(Muller et al., 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the failure of γ-tubulin to localize to the spindle, without affecting its localization to the pole, and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

gamma-tubulin binding

inferred from direct assay

(Gunawardane et al., 2003)

Biological Process

CV term

Evidence

References

mitosis

inferred from mutant phenotype

(Verollet et al., 2006)

mitotic spindle organization and biogenesis

inferred from mutant phenotype

(Goshima et al., 2007)

regulation of cell cycle

inferred from mutant phenotype

(Muller et al., 2006)

Cellular Component

CV term

Evidence

References

gamma-tubulin ring complex

non-traceable author statement

(Gunawardane, 2001.12.18)

inferred from direct assay

(Gunawardane et al., 2003)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Grip71 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61124

Orthologs

Genome-wide drosophilid orthologs

Dana\GF15082

Dere\GG21129

Dmoj\GI15799

Dper\GL15760

Dpse\GA10262

Dsec\GM17290

Dsim\GD24153

Dvir\GJ10275

Dwil\GK18208

Dyak\GE13203

Curated drosophilid orthologs

Dpse\GA10262

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010083
Anopheles gambiae (Ensembl)
ENSCINP00000010015
Ciona intestinalis (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v31228

w¹¹¹⁸; P{GD6950}v31228

v31229

w¹¹¹⁸; P{GD6950}v31229/CyO

Bloomington

22406

y¹ w^67c23; P{EPgy2}Grip71^EY20619

Genomic Clones ( 2 )

cDNA Clones ( 17 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0032705

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0032705

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Grip71 CG10346 Source for identity of Grip71 CG10346 was sequence comparison (date:030311).

(FlyBase, 1996-)

Source for database merge of

Additional comments

Comments About Role

Grip71 may act as a scaffold via its WD repeats in the gamma-tubulin ring complex. Unlike other subunits of the complex, Grip71 does not promote GTP binding to γ-tubulin.

(Gunawardane et al., 2003)

Comments About Molecular Function

Grip71 associates with Grip163, l(1)dd4, Grip128, Grip84 and γ-Tubulin.

(Gunawardane et al., 2003)

Other Comments

Identification: Protein identified as part of the γ tubulin ring complex.

(Gunawardane et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_136058

RefSeq (Proteins)

•

NP_609902

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WD40/YVTN repeat-like (IPR015943)

WD40 repeat (IPR001680)

WD40 repeat-like (IPR011046)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG10346

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61124

DEDB - Drosophila exon database: splicing graphs

•

7993

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0032705

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0032705

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0032705

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0032705

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0032705

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0032705

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0032705

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010083
Anopheles gambiae (Ensembl)
ENSCINP00000010015
Ciona intestinalis (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0032705

University of Chicago Dev. Expression

•

FBgn0032705

Synonyms & Secondary IDs ( 4 )

Reported As

Symbol Synonym

CG10346

(Giot et al., 2003, Goshima et al., 2007)

Dgp71WD

(Gunawardane et al., 2003, Verollet et al., 2006)

Dgrip71

(Stumpff et al., 2005, Gunawardane, 2001.12.18, Goshima et al., 2007, Goshima et al., 2007)

Grip71

(Muller et al., 2006, )

Name Synonym

Grip71

Secondary FlyBase IDs

References ( 15 )

Research paper

Goshima et al., 2007, Science 316(5823): 417--421: Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0201458]
Muller et al., 2006, Science 314(5799): 654--657: A centrosome-independent role for γ-TuRC proteins in the spindle assembly checkpoint. [FBrf0194177]
Verollet et al., 2006, J. Cell Biol. 172(4): 517--528: Drosophila melanogaster [gamma]-TuRC is dispensable for targeting [gamma]-tubulin to the centrosome and microtubule nucleation. [FBrf0190946]
Stumpff et al., 2005, Curr. Biol. 15(17): 1525--1534: Drosophila Wee1 Interacts with Members of the gammaTURC and Is Required for Proper Mitotic-Spindle Morphogenesis and Positioning. [FBrf0187336]
Gunawardane et al., 2003, Molec. Biol. Cell 14(3): 1017--1026: Characterization of a new gammaTuRC subunit with WD repeats. [FBrf0159185]

Supplementary material

Goshima et al., 2007, Science 316(5823):: Supporting online material for Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0200397]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Bellen, 2006.8.3, MBBatch686_2.xml: MBBatch686_2.xml [FBrf0191805]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Gunawardane, 2001.12.18, GenBank/EMBL/DDBJ: AF461267: Drosophila melanogaster gamma-tubulin ring complex subunit Dgrip71 mRNA, complete cds. [FBrf0157675]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]