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Gene Dmel\RPA2

General Information
Symbol	Dmel\RPA2	Species	D. melanogaster
Name	Replication protein A2	Annotation symbol	CG9273
Feature type	protein_coding_gene	FlyBase ID	FBgn0032906
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	38F6-38F6	Sequence location	2L:20,924,975..20,926,144 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Replication protein A2 is referred to in FlyBase by the symbol Dmel\RPA2 (CG9273, FBgn0032906). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: neurogenesis. 7 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Nucleic acid-binding, OB-fold; Nucleic acid-binding, OB-fold-like; Replication protein A, C-terminal; Replication protein A, subunit RPA32; Winged helix-turn-helix transcription repressor DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval hindgut, larval salivary gland, larval trachea, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1639224_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RPA2. Gene sequence location is 2L:20924975..20926144.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA03184 BKN28305 Controlled Vocabulary Terms neurogenesis
FB2011_10	References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38F6-38F6   Limits computationally determined from genome sequence between P{PZ}l(2)38EFd04530 and P{PZ}l(2)0528705287
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RPA2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RPA2-RA FBtr0081438  NM_136233   960   246
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RPA2-PA   FBpp0080967   26.4   246   4.72     NP_610077   AAF53948
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Nucleic acid-binding, OB-fold (IPR012340) Replication protein A, subunit RPA32 (IPR014646) Replication protein A, C-terminal (IPR014892) Nucleic acid-binding, OB-fold-like (IPR016027)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140851   AA696041   AE014134 AAF53948   AI295971   AI295979   AW941238   AY069259 AAL39404   AY089651 AAL90389   BH214576   BI237400   BI355572   BI356012   BI369094   BI570791   BI571162   BI571234   BI572095   BI572349   BI572359   BI590964   BI591799   BI606755   BI633865   BI639577   BP547123   CO181999   CO194717   CO274908   CO276806   CO292419   CO293022   CO296318   EC063629   EC071681   EC072891   EC082863   EC224876   EC247096   FD585719   FD586521   FD586649   FD587265   FD587282   GH739108   GH739615   GH741940   GH744031   GH746342   GH747303   GH764662   GH768146   GO254376
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SXG3 Q9VIH1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0032906    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0032906 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0032906 expression level Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8777) embryo 02-04hr  (5199) embryo 04-06hr  (7639) embryo 06-08hr  (6366) embryo 08-10hr  (4682) embryo 10-12hr  (2747) embryo 12-14hr  (1212) embryo 14-16hr  (1287) embryo 16-18hr  (1116) embryo 18-20hr  (766) embryo 20-22hr  (684) embryo 22-24hr  (617) larva L1  (1442) larva L2  (1455) larva L3 12hr old  (1060) larva L3 puffstage 1-2  (1256) larva L3 puffstage 3-6  (1613) larva L3 puffstage 7-9  (1844) white prepupae new  (1826) white prepupae 12hr  (2420) white prepupae 24hr  (1899) pupae 2d postWPP  (1074) pupae 3d postWPP  (669) pupae 4d postWPP  (779) adult male 01day  (647) adult male 05day  (852) adult male 30day  (864) adult female 01day  (2658) adult female 05day  (4559) adult female 30day  (4919) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8777) embryo 02-04hr  (5199) embryo 04-06hr  (7639) embryo 06-08hr  (6366) embryo 08-10hr  (4682) embryo 10-12hr  (2747) embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1  1442 larva L2  1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6  (1613) larva L3 puffstage 7-9  (1844) white prepupae new  (1826) white prepupae 12hr  (2420) white prepupae 24hr  (1899) pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day  (2658) adult female 05day  (4559) adult female 30day  (4919) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0032906 expression level Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8777) embryo 02-04hr  (5199) embryo 04-06hr  (7639) embryo 06-08hr  (6366) embryo 08-10hr  (4682) embryo 10-12hr  (2747) embryo 12-14hr  (1212) embryo 14-16hr  (1287) embryo 16-18hr  (1116) embryo 18-20hr  (766) embryo 20-22hr  (684) embryo 22-24hr  (617) larva L1  (1442) larva L2  (1455) larva L3 12hr old  (1060) larva L3 puffstage 1-2  (1256) larva L3 puffstage 3-6  (1613) larva L3 puffstage 7-9  (1844) white prepupae new  (1826) white prepupae 12hr  (2420) white prepupae 24hr  (1899) pupae 2d postWPP  (1074) pupae 3d postWPP  (669) pupae 4d postWPP  (779) adult male 01day  (647) adult male 05day  (852) adult male 30day  (864) adult female 01day  (2658) adult female 05day  (4559) adult female 30day  (4919) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8777) embryo 02-04hr  (5199) embryo 04-06hr  (7639) embryo 06-08hr  (6366) embryo 08-10hr  (4682) embryo 10-12hr  (2747) embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1  1442 larva L2  1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6  1613 larva L3 puffstage 7-9  1844 white prepupae new  1826 white prepupae 12hr  2420 white prepupae 24hr  1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day  (2658) adult female 05day  (4559) adult female 30day  (4919) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8777 embryo 02-04hr    5199 embryo 04-06hr    7639 embryo 06-08hr    6366 embryo 08-10hr    4682 embryo 10-12hr    2747 embryo 12-14hr    1212 embryo 14-16hr    1287 embryo 16-18hr    1116 embryo 18-20hr    766 embryo 20-22hr    684 embryo 22-24hr    617 larva L1    1442 larva L2    1455 larva L3 12hr old    1060 larva L3 puffstage 1-2    1256 larva L3 puffstage 3-6    1613 larva L3 puffstage 7-9    1844 white prepupae new    1826 white prepupae 12hr    2420 white prepupae 24hr    1899 pupae 2d postWPP    1074 pupae 3d postWPP    669 pupae 4d postWPP    779 adult male 01day    647 adult male 05day    852 adult male 30day    864 adult female 01day    2658 adult female 05day    4559 adult female 30day    4919 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0032906    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0032906 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval hindgut, larval salivary gland, larval trachea, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0032906 expression level Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary  (709.5) Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0032906 expression level Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary  709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    514.025 Larval Midgut    47.2 Larval Hindgut    105.4 Larval Malpighian Tubules    49.6 Larval Fat Body    55.4 Larval Salivary Gland    195.2 Larval Trachea    250.1 Larval Carcass    115.625 Adult Head    30 Adult Eye    30.8 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    30.5 Adult Midgut    38.6 Adult Hindgut    31.9 Adult Malpighian Tubules    29.6 Adult Fat Body    26.4 Adult Salivary Gland    19.7 Adult Heart    31.175 Adult VirginFemale Spermatheca    28.1 Adult InseminatedFemale Spermatheca    26.4 Adult Ovary    709.5 Adult Testis    94.7 Adult Male Accessory Gland no informative data Adult Carcass    31.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    30   NA  Eye    30.8   NA  Brain    40.7   514.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    59.3   NA  Crop    30.5   47.2  Midgut    38.6   105.4  Hindgut    31.9   49.6  Malpighian Tubules    29.6   55.4  Fat Body    26.4   195.2  Salivary Gland    19.7   NA  Heart    31.175   250.1  Trachea    NA   NA  VirginFemale Spermatheca    28.1   NA  InseminatedFemale Spermatheca    26.4   NA  Ovary    709.5   NA  Testis    94.7   NA  Male Accessory Gland    no informative data   115.625  Carcass    31.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032906 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9273-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for RPA2 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 RPA2GD4430 lethal | recessive | second instar larval stage RPA22 RPA23 RPA2KG00759 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 RPA2GD4430 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 RPA2GD4430 visible, with Scer\GAL4pnr-MD237 RPA2GD4430 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage, with Scer\GAL41407 RPA2GD4430 ganglion mother cell | larval stage, with Scer\GAL41407 RPA2GD4430 mesothoracic tergum, with Scer\GAL4pnr-MD237 RPA2GD4430
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of RPA2 Class Mutagen Stocks Known lesion RPA2KG00759 amorphic allele - genetic evidence P-element activity 2 Yes RPA2KG00771b P-element activity 1 Yes RPA22 amorphic allele - genetic evidence Delta2-3 0 Yes RPA23 amorphic allele - genetic evidence Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RPA2 Class Mutagen Stocks Known lesion RPA2GD4430 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes RPA2HMS01061 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RPA2KK108305 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7080 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4430} NA P{KK108305} NA P{TRiP.HMS01061} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}RPA2KG00759 NA P{SUPor-P}RPA2KG00771b NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from sequence or structural similarity with Saccharomyces RFA2 (FlyBase, 1992-)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from sequence or structural similarity with Saccharomyces RFA2 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
DNA replication factor A complex inferred from sequence or structural similarity with Saccharomyces RFA2 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RPA2 allele Gene References unspecified CycB (Crest et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61322 DroID - A comprehensive database of gene and protein interactions. • FBgn0032906 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35364
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24241 Dere\GG21537 Dgri\GH13058 Dmoj\GI18202 Dper\GL19582 Dpse\GA21663 Dsec\GM23291 Dsim\GD21666 Dvir\GJ14710 Dwil\GK24313 Dyak\GE13014
Curated drosophilid orthologs	Dpse\GA21663   Dsim\CG9273 (Landry et al., 2005)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL014250-PA Aedes aegypti (mosquito) AGAP008332-PA Anopheles gambiae (mosquito) XP_001121269.1 Apis mellifera (honey bee) XP_001947814.1 Acyrthosiphon pisum (pea aphid) At2g24490.1 Arabidopsis thaliana (wall cress) 33168 Batrachochytrium dendrobatidis (chytrid fungus) 249627 Branchiostoma floridae (lancelet) BGIBMGA005434-PA Bombyx mori (silk worm) ENSBTAP00000006592 Bos taurus (bovine) ENSBTAP00000008174 Bos taurus (bovine) CAL0006323 Candida albicans (a form of yeast) ENSCAFP00000017644 Canis familiaris (dog) CAGL0M03993g Candida glabrata (haploid yeast) ENSCINP00000025107 Ciona intestinalis (sea squirt) cn04199 Cryptococcus neoformans (yeast-like fungus) CPIJ006691 Culex pipens (mosquito) ENSCPOP00000007868 Cavia porcellus (guinea pig) 186935 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000017849 Ciona savignyi (sedentary tunicate) 218725 Capitella spl (polychaete worm) DEHA2F23892g Debaryomyces Hansenii (yeast) 226387 Daphnia pulex (daphnia) ENSDARP00000054077 Danio rerio (zebra fish) ENSECAP00000004230 Equus caballus (horse) ENSGACP00000018385 Gymnopilus aculeatus (mushroom) ENSGALP00000001048 Gallus gallus (chicken) 170399 Hypsipyla robusta (cedar shoot borer) ENSP00000363015 Homo sapiens (human) KLLA0E07371g Kluyveromyces lactis (yeast) 237715 Lottia gigantea (owl limpet) ENSMODP00000013450 Monodelphis domestica (gray opossum) ENSMODP00000013475 Monodelphis domestica (gray opossum) ENSMMUP00000023898 Macaca mulatta (rhesus monkey) ENSMUSP00000099621 Mus musculus (house mouse) XP_001603125.1 Nasonia vitripennis (wasp) ENSOANP00000006077 Ornithorynchus anatinus (platypus) ENSORLP00000017942 Oryzias latipes (japanese killifish) LOC_Os02g58220.1 Oryza sativa (asian rice) 36144 Ostreococcus tauri (green alga) PHUM007972-PA Pediculus humanus (human body louse) 199215 Physcomitrella patens (moss) ENSPPYP00000001907 Pongo pygmaeus (orangutan) 79428 Phytophthora ramorum (sudden oak death pathogen) 743384 Populus trichocarpa (black cottonwood) ENSPTRP00000052613 Pan troglodytes (chimpanzee) ENSRNOP00000017549 Rattus norvegicus (norway rat) 5005121 Sorghum bicolor (sorghum) YNL312W Saccharomyces cerevisiae (budding yeast) Smp_080530 Schistosoma mansoni (trematode parasite) SPCC1753.01c Schizosaccharomyces pombe (fission yeast) 32825 Trichoplax adhaerens (metazoa) XP_970140.1 Tribolium castaneum (red flour beetle) ENSTNIP00000001752 Tetraodon nigroviridis (green spotted pufferfish) 262513 Thalassiosira pseudonana (marine diatom) ENSTRUP00000024017 Takifugu rubripes (pufferfish Takifugu) 80459.m00182 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000003870 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13909 LH13952 LH11411 FBgn0230722 FBgn0226273 FBgn0201917 FBgn0193089 FBgn0178158 JGI_V11_226387 JGI_V11_239719 FBgn0081648 FBgn0157180 FBgn0140941 FBgn0120535 FBgn0113715 FBgn0101235 CPIJ006417 CPIJ006691 BGIBMGA005434 ACYPI003814 GB13976 AGAP008332 AAEL014250 PB21406 PHUM249390 GLEAN_05650 GLEAN_15098
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	34587 y1 sc* v1; P{TRiP.HMS01061}attP2 12907 y1 w67c23; P{SUPor-P}RPA2KG00759/CyO 12882 y1 P{SUPor-P}rdgAKG00771a; P{SUPor-P}RPA2KG00771b
Kyoto	114619 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{SUPor-P}RPA2KG00759 P{neoFRT}40A/CyO y+
VDRC	v102306 P{KK108305}VIE-260B v30570 w1118; P{GD4430}v30570 v30572 w1118; P{GD4430}v30572
Genomic Clones ( 1 )
	BACR06G10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 47 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM04951 RH07841
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM31409 LP09749 LP09758 RE33624 RE37088 RE55178 RH04659 RH05573 RH05986 RH06071 RH06779 RH07438 RH07852 RH73557 SD22204 SD28041
Other clones	965 33494 71794 72856 82384 CK01.142C.C11 CK00516 EC19976 EC39105 EK115910 EK122115 EK200140 EK201525 EK213353 FGH113F08 RM03492 RM03692 RM03792 RM03992 RM04092 RP01211 RP001004066 RP001012760 RP001019474 RP001039655 RP001047178 RP001050177 RP001052845 RP001054978
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0032906 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35364
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RPA2 CG9273 (Crest et al., 2007)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136233 RefSeq (Proteins) • NP_610077 DDBJ / EMBL / GenBank • AA696041 AAF53948 AAL39404 AAL90389 AW941238 AY069259 AY089651 BH214576 BI572349 Entrez Gene - A searchable database of RefSeq genes. • 35364 UniProtKB/TrEMBL • Q8SXG3 Q9VIH1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Nucleic acid-binding, OB-fold (IPR012340) Replication protein A, subunit RPA32 (IPR014646) Replication protein A, C-terminal (IPR014892) Nucleic acid-binding, OB-fold-like (IPR016027)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61322 DroID - A comprehensive database of gene and protein interactions. • FBgn0032906 DRSC - Results from RNAi screens. • FBgn0032906 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032906 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9273-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0032906 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35364 InParanoid A subset of ortholog calls from InParanoid. • AAEL014250-PA Aedes aegypti (mosquito) AGAP008332-PA Anopheles gambiae (mosquito) XP_001121269.1 Apis mellifera (honey bee) XP_001947814.1 Acyrthosiphon pisum (pea aphid) At2g24490.1 Arabidopsis thaliana (wall cress) 33168 Batrachochytrium dendrobatidis (chytrid fungus) 249627 Branchiostoma floridae (lancelet) BGIBMGA005434-PA Bombyx mori (silk worm) ENSBTAP00000006592 Bos taurus (bovine) ENSBTAP00000008174 Bos taurus (bovine) CAL0006323 Candida albicans (a form of yeast) ENSCAFP00000017644 Canis familiaris (dog) CAGL0M03993g Candida glabrata (haploid yeast) ENSCINP00000025107 Ciona intestinalis (sea squirt) cn04199 Cryptococcus neoformans (yeast-like fungus) CPIJ006691 Culex pipens (mosquito) ENSCPOP00000007868 Cavia porcellus (guinea pig) 186935 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000017849 Ciona savignyi (sedentary tunicate) 218725 Capitella spl (polychaete worm) DEHA2F23892g Debaryomyces Hansenii (yeast) 226387 Daphnia pulex (daphnia) ENSDARP00000054077 Danio rerio (zebra fish) ENSECAP00000004230 Equus caballus (horse) ENSGACP00000018385 Gymnopilus aculeatus (mushroom) ENSGALP00000001048 Gallus gallus (chicken) 170399 Hypsipyla robusta (cedar shoot borer) ENSP00000363015 Homo sapiens (human) KLLA0E07371g Kluyveromyces lactis (yeast) 237715 Lottia gigantea (owl limpet) ENSMODP00000013450 Monodelphis domestica (gray opossum) ENSMODP00000013475 Monodelphis domestica (gray opossum) ENSMMUP00000023898 Macaca mulatta (rhesus monkey) ENSMUSP00000099621 Mus musculus (house mouse) XP_001603125.1 Nasonia vitripennis (wasp) ENSOANP00000006077 Ornithorynchus anatinus (platypus) ENSORLP00000017942 Oryzias latipes (japanese killifish) LOC_Os02g58220.1 Oryza sativa (asian rice) 36144 Ostreococcus tauri (green alga) PHUM007972-PA Pediculus humanus (human body louse) 199215 Physcomitrella patens (moss) ENSPPYP00000001907 Pongo pygmaeus (orangutan) 79428 Phytophthora ramorum (sudden oak death pathogen) 743384 Populus trichocarpa (black cottonwood) ENSPTRP00000052613 Pan troglodytes (chimpanzee) ENSRNOP00000017549 Rattus norvegicus (norway rat) 5005121 Sorghum bicolor (sorghum) YNL312W Saccharomyces cerevisiae (budding yeast) Smp_080530 Schistosoma mansoni (trematode parasite) SPCC1753.01c Schizosaccharomyces pombe (fission yeast) 32825 Trichoplax adhaerens (metazoa) XP_970140.1 Tribolium castaneum (red flour beetle) ENSTNIP00000001752 Tetraodon nigroviridis (green spotted pufferfish) 262513 Thalassiosira pseudonana (marine diatom) ENSTRUP00000024017 Takifugu rubripes (pufferfish Takifugu) 80459.m00182 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000003870 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35364 modMine - Data generated by the modENCODE project. • FBgn0032906 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13909 LH13952 LH11411 FBgn0230722 FBgn0226273 FBgn0201917 FBgn0193089 FBgn0178158 JGI_V11_226387 JGI_V11_239719 FBgn0081648 FBgn0157180 FBgn0140941 FBgn0120535 FBgn0113715 FBgn0101235 CPIJ006417 CPIJ006691 BGIBMGA005434 ACYPI003814 GB13976 AGAP008332 AAEL014250 PB21406 PHUM249390 GLEAN_05650 GLEAN_15098
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	153194_at (Kristensen et al., 2005) CG9273 (Crest et al., 2007, Proschel et al., 2006, Somma et al., 2008, Keleman et al., 2009.8.5, Wittkopp et al., 2008, Ni et al., 2010.12.1) dRPA2 (Crest et al., 2007) RPA2 (Neumüller et al., 2011)
Name Synonym	CG9273 (Somma et al., 2008) Replication protein A2   replication protein A, subunit 2 (Crest et al., 2007)
Secondary FlyBase IDs
References ( 26 )
Generate a list of	All references relating to this gene ( 26 )
List References by type	Research paper  ( 8 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...