FlyBase Gene Report: Dmel\kune

General Information

Symbol

Dmel\kune

Species

D. melanogaster

Name

kune-kune

Annotation Symbol

CG1298

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033032

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

41F6-41F6

Sequence location

2R:5,671,741..5,672,800 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:5671741..5672800

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in establishment of glial blood-brain barrier

inferred from mutant phenotype

(Nelson et al., 2010)

involved_in regulation of tube length, open tracheal system

inferred from mutant phenotype

(Nelson et al., 2010)

involved_in septate junction assembly

inferred from mutant phenotype

(Nelson et al., 2010)

NOT involved_in establishment of glial blood-brain barrier

inferred from mutant phenotype

(Stork et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in pleated septate junction

inferred from direct assay

(Izumi et al., 2012)

located_in septate junction

inferred from direct assay

(Nelson et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR004031

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

located_in septate junction

inferred from sequence or structural similarity with FLYBASE:sinu; FB:FBgn0010894

(Wu et al., 2004)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

PMP-22/EMP/MP20/Claudin superfamily

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\kune for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

kune-RA

FBtr0086037

NM_136335

1060

264

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

kune-PA

FBpp0085373

29.8

264

4.83

NP_610179

AAF57321

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PMP-22/EMP/MP20/Claudin superfamily

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kune using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

kune_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

atrium | precursor

Comment: reported as atrium primordium

(Fisher et al., 2012)

clypeo-labral primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic/larval epidermis

(Nelson et al., 2010)

embryonic/larval salivary gland

(Nelson et al., 2010)

embryonic/larval trachea

(Nelson et al., 2010)

embryonic/larval hindgut

(Nelson et al., 2010)

embryonic/larval foregut

(Nelson et al., 2010)

embryonic/larval glial cell

(Nelson et al., 2010)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

kune is highly expressed in ectodermally-derived tissues including epidermis, salivary gland, trachea, hindgut, and foregut. It is also expressed in glial cells. It was localized to septate junctions.

(Nelson et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in pleated septate junction

inferred from direct assay

(Izumi et al., 2012)

located_in septate junction

inferred from direct assay

(Nelson et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kune in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\kune

View exonic expression by tissue for Dmel\kune

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033032

DRscDB

35504/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0033032

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1298-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1298

Flygut - An atlas of the Drosophila adult midgut

FBgn0033032

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\kune

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of kune

Transgenic constructs containing regulatory region of kune

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2R)M41A8