FB2019_05, released Oct 15, 2019

Gene: Dmel\kune

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General Information
Symbol
Dmel\kune
Species
D. melanogaster
Name
kune-kune
Annotation Symbol
CG1298
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033032
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Key Links
Genomic Location
Cytogenetic map
41F6-41F6
Sequence location
2R:5,671,741..5,672,800 [+]
Recombination map
2-55
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\kune or the JBrowse view of Dmel\kune for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
kune-RA
FBtr0086037
NM_136335
1060
264
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
kune-PA
FBpp0085373
29.8
264
4.83
NP_610179
AAF57321
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kune using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (6 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
establishment of glial blood-brain barrier
inferred from mutant phenotype
(Nelson et al., 2010)
NOT establishment of glial blood-brain barrier
inferred from mutant phenotype
(Stork et al., 2008)
regulation of tube length, open tracheal system
inferred from mutant phenotype
(Nelson et al., 2010)
septate junction assembly
inferred from mutant phenotype
(Nelson et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
pleated septate junction
inferred from direct assay
(Izumi et al., 2012)
septate junction
inferred from direct assay
(Nelson et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
integral component of membrane
inferred from electronic annotation with InterPro:IPR004031
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
septate junction
inferred from sequence or structural similarity with FLYBASE:sinu; FB:FBgn0010894
(Wu et al., 2004)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
embryonic/larval epidermis
(Nelson et al., 2010)
embryonic/larval salivary gland
(Nelson et al., 2010)
embryonic/larval trachea
(Nelson et al., 2010)
embryonic/larval hindgut
(Nelson et al., 2010)
embryonic/larval foregut
(Nelson et al., 2010)
embryonic/larval glial cell
(Nelson et al., 2010)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
kune is highly expressed in ectodermally-derived tissues including epidermis, salivary gland, trachea, hindgut, and foregut. It is also expressed in glial cells. It was localized to septate junctions.
(Nelson et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
pleated septate junction
inferred from direct assay
(Izumi et al., 2012)
septate junction
inferred from direct assay
(Nelson et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kune in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of kune
Transgenic constructs containing regulatory region of kune
Deletions and Duplications ( 7 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (1)
Drer\lim2.4
1 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\str-73
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190EN2 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\kune
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00007685
Drosophila simulans
Dsim\GD10381
Drosophila sechellia
Dsec\GM16516
Drosophila erecta
Dere\GG23175
Drosophila yakuba
Dyak\GE20527
Drosophila ananassae
Dana\GF14554
Drosophila pseudoobscura pseudoobscura
Dpse\GA11952
Drosophila persimilis
Dper\GL11055
Drosophila willistoni
Dwil\GK10757
Drosophila virilis
Dvir\GJ21915
Drosophila mojavensis
Dmoj\GI18881
Drosophila grimshawi
Dgri\GH20456
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BZO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015369
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11378
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005237
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005088
Anopheles darlingi
American malaria mosquito
Adar\ADAC001726
Anopheles gambiae
Malaria mosquito
Agam\AGAP003810
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ011112
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0FFW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007748
Bombyx mori
Silkmoth
Bmor\BGIBMGA007749
Bombyx mori
Silkmoth
Bmor\BGIBMGA007750
Danaus plexippus
Monarch butterfly
Dple\DPOGS208653-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS208654-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS208652-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003336
Apis florea
Little honeybee
Aflo\2345803012
Apis florea
Little honeybee
Aflo\2345802852
Apis florea
Little honeybee
Aflo\2345802948
Apis mellifera
Western honey bee
Amel\GB54254
Apis mellifera
Western honey bee
Amel\GB54253
Apis mellifera
Western honey bee
Amel\GB54255
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10685
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22750
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10686
Bombus terrestris
Buff-tailed bumblebee
Bter\15215571
Bombus terrestris
Buff-tailed bumblebee
Bter\15213433
Bombus terrestris
Buff-tailed bumblebee
Bter\15213435
Bombus terrestris
Buff-tailed bumblebee
Bter\15208512
Linepithema humile
Argentine ant
Lhum\LH20695
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002508
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002509
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002507
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015605
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015604
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015606
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10459
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10460
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10458
Tribolium castaneum
Red flour beetle
Tcas\TC014460
Tribolium castaneum
Red flour beetle
Tcas\TC014459
Tribolium castaneum
Red flour beetle
Tcas\TC014461
Pediculus humanus
Human body louse
Phum\PHUM302480
Pediculus humanus
Human body louse
Phum\PHUM535700
Rhodnius prolixus
Kissing bug
Rpro\RPRC005446
Rhodnius prolixus
Kissing bug
Rpro\RPRC002219
Rhodnius prolixus
Kissing bug
Rpro\RPRC005311
Cimex lectularius
Bed bug
Clec\CLEC003773
Cimex lectularius
Bed bug
Clec\CLEC009882
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004344
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI34966
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006751
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI008414
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006541
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI30590
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI43414
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI47418
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05355
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05354
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05353
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0FFJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012193
Strigamia maritima
European centipede
Smar\SMAR009174
Ixodes scapularis
Black-legged tick
Isca\ISCW023960
Ixodes scapularis
Black-legged tick
Isca\ISCW023959
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60034.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM64544.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g10220
Tetranychus urticae
Two-spotted spider mite
Turt\tetur01g06880
Tetranychus urticae
Two-spotted spider mite
Turt\tetur01g06860
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_300494
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_203100
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0ORL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_1786
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Htr6L_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Adrb2L_19
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Adrb2L_7
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
pck
3 of 10
sinu
3 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    kune - AGO1
    anti bait coimmunoprecipitation, quantitative reverse transcription pcr
    (Hong et al., 2009)
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-55
    Cytogenetic map
    41F6-41F6
    Sequence location
    2R:5,671,741..5,672,800 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    41F6-41F6
    Limits computationally determined from genome sequence between P{PZ}vlc07022&P{lacW}l(2)09851k08138 and P{PZ}l(2)0985109851&P{lacW}Src42Ak10108
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    2-55
    (FlyBase, 2016)
    2-55.7
    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (6)
    Genomic Clones (16)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (30)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      polyclonal
      (Hildebrandt et al., 2015, Nelson et al., 2010)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: kune CG1298
      (Nelson et al., 2010)
      Source for database merge of
      Additional comments
      Other Comments
      Origin and Etymology
      Discoverer
      Etymology
      The gene is named "kune-kune", Japanese for "wiggling like a snake" based on the mutant tracheal phenotype.
      (Nelson et al., 2010)
      Identification
      External Crossreferences and Linkouts ( 27 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Synonyms and Secondary IDs (5)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (43)