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Gene Dmel\scaf

General Information
Symbol	Dmel\scaf	Species	D. melanogaster
Name	scarface	Annotation symbol	CG11066
Feature type	protein_coding_gene	FlyBase ID	FBgn0033033
Gene Model Status	Current	Stock availability	6 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	41F6-41F8	Sequence location	2R:1,564,255..1,579,228 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene scarface is referred to in FlyBase by the symbol Dmel\scaf (CG11066, FBgn0033033). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: serine-type endopeptidase activity. There is experimental evidence that it is involved in the biological process: imaginal disc-derived male genitalia morphogenesis. 11 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/first instar larval cuticle; adult head. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Peptidase S1; Peptidase S1A, chymotrypsin-type; Trypsin-like cysteine/serine peptidase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval trachea, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult brain, adult crop, larval hindgut, inseminated spermatheca. Comments on Affy2 ProbeSet: ProbeSet 1640912_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of scarface. Gene sequence location is 2R:1564255..1579228.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 41F6-41F8   Limits computationally determined from genome sequence between P{PZ}vlc07022&P{lacW}l(2)09851k08138 and P{PZ}l(2)0985109851&P{lacW}Src42Ak10108
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\scaf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) scaf-RA FBtr0086061  NM_165441   3390   655 scaf-RB FBtr0086060  NM_136336   3043   655
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein scaf-PA   FBpp0085397   71.4   655   7.05     NP_724424   AAF57320 scaf-PB   FBpp0085396   71.4   655   7.05     NP_610180   AAM68354
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567382   AE013599 AAF57320   AE013599 AAM68354   AI107997   AI259028   AI259908   AI294558   AI517939   AI534052   AY047548 AAK77280   BE976927   BG639800   BH759575   BI234477   BI567959   BI613880   BI617747   BI617835   CA805849   CA807428   CO190977   CO264466   CO265399   CO268290   CO269912   CO270079   CO274513   CO280082   CO280744   CO280777   CO284186   CO285381   CO287341   CO298869   CO299483   CO300592   CO301233   CO306367   CO307311   CO307320   CO312928   CO317371   CO318544   CO321358   CO322925   CO331495   CO332994   CO333004   CZ469325   CZ483805   CZ483806   EC059963   EC061697   EC062742   EC063132   EC065073   EC066092   EC067001   EC068492   EC068690   EC071065   EC071216   EC071250   EC072395   EC072499   EC073238   EC076871   EC085652   EC088951   EC089754   EC090844   EC195768   EC203329   EC203405   EC205057   EC205325   EC210191   EC210719   EC211192   EC213646   EC215847   EC216483   EC216668   EC220109   EC220911   EC222273   EC222632   EC246423   EC249934   ES695606   ES695610   ES695611   ES695613   ES695631   ES695656   ES695669   ES695681   ES695691   GH740294   GH742351   GH742676   GH742878   GH742968   GH743461   GH746181   GH746498   GH761724   GH762297   GH762452   GH762486   GH763638   GH764574   GH765159   GH765741   GH766085   GH766523   GH766690   GH769185
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K5M0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic leading edge cell   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval pericardial cell   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
extracellular space inferred from direct assay (Rousset et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval trachea, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult brain, adult crop, larval hindgut, inseminated spermatheca. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass    1760.95 Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea  516.025 Larval Carcass  (1760.95) Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass  (1760.95) Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass    1760.95 Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass    1760.95 Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea  516.025 Larval Carcass  (1760.95) Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass  (1760.95) Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    98.025 Larval Midgut    12.7 Larval Hindgut    142.1 Larval Malpighian Tubules    4.2 Larval Fat Body    5.2 Larval Salivary Gland    15.7 Larval Trachea    516.025 Larval Carcass    1760.95 Adult Head    357 Adult Eye    325.225 Adult Brain    196.2 Adult Thoracic-Abdominal Ganglion    16.9 Adult Crop    395.6 Adult Midgut    3.5 Adult Hindgut    8.7 Adult Malpighian Tubules    4.8 Adult Fat Body    8.6 Adult Salivary Gland    11.8 Adult Heart    8.25 Adult VirginFemale Spermatheca    64.7 Adult InseminatedFemale Spermatheca    141.7 Adult Ovary    12.7 Adult Testis    47.5 Adult Male Accessory Gland    7.3 Adult Carcass    76.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    357   NA  Eye    325.225   NA  Brain    196.2   98.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    16.9   NA  Crop    395.6   no informative data  Midgut    3.5   142.1  Hindgut    8.7   4.2  Malpighian Tubules    4.8   5.2  Fat Body    8.6   15.7  Salivary Gland    no informative data   NA  Heart    8.25   516.025  Trachea    NA   NA  VirginFemale Spermatheca    64.7   NA  InseminatedFemale Spermatheca    141.7   NA  Ovary    no informative data   NA  Testis    47.5   NA  Male Accessory Gland    7.3   1760.95  Carcass    76.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female  (29) head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male  (34) head, mated 4-day male  (27) head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8  (30) carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female  29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male  (34) head, mated 4-day male  27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8  30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    29 head, virgin 4-day female    14 head, virgin 20-day female    15 head, mated 1-day female    21 head, mated 4-day female    13 head, mated 20-day female    13 head, mated 1-day male    34 head, mated 4-day male    27 head, mated 20-day male    21 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    30 carcass, larvae L3 wandering    18 carcass, 1-day adult    6 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr    115 embryo 18-20hr    193 embryo 20-22hr    104 embryo 22-24hr    113 larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr  (115) embryo 18-20hr  (193) embryo 20-22hr  (104) embryo 22-24hr  (113) larva L1  (33) larva L2  (68) larva L3 12hr old  (48) larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr  (32) white prepupae 24hr  (94) pupae 2d postWPP    25 pupae 3d postWPP  (43) pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr  (115) embryo 18-20hr  (193) embryo 20-22hr  104 embryo 22-24hr  (113) larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr    115 embryo 18-20hr    193 embryo 20-22hr    104 embryo 22-24hr    113 larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr    115 embryo 18-20hr    193 embryo 20-22hr    104 embryo 22-24hr    113 larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr  (115) embryo 18-20hr  (193) embryo 20-22hr  (104) embryo 22-24hr  (113) larva L1  (33) larva L2  (68) larva L3 12hr old  (48) larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr  32 white prepupae 24hr  (94) pupae 2d postWPP    25 pupae 3d postWPP  (43) pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr  115 embryo 18-20hr  (193) embryo 20-22hr  104 embryo 22-24hr  113 larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    16 embryo 14-16hr    21 embryo 16-18hr    115 embryo 18-20hr    193 embryo 20-22hr    104 embryo 22-24hr    113 larva L1    33 larva L2    68 larva L3 12hr old    48 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    10 white prepupae 12hr    32 white prepupae 24hr    94 pupae 2d postWPP    25 pupae 3d postWPP    43 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    11 adult male 30day    10 adult female 01day    8 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (86) Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8  (31) wing disc ML-DmD9    8 wing disc ML-DmD16-c3  (35) wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2  (53) ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (86) Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8  31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3  (35) wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2  (53) ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    1 embryonic 1182-4H    20 embryonic GM2    0 embryonic Kc167    3 embryonic S1    6 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    11 wing disc ML-DmD8    31 wing disc ML-DmD9    8 wing disc ML-DmD16-c3    35 wing disc ML-DmD21    1 wing disc ML-DmD32    21 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    5 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    53 ovary fGS/OSS    14 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3  (33) Rotenone 8 μg 12 hrs, larvae L3  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3  (33) Rotenone 8 μg 12 hrs, larvae L3  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    24 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_29
External Data & Images
Linkouts	BDGP expression data • GH05918 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033033 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11066-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for scaf Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage | recessive scafPBss lethal | recessive scaf27 partially lethal - majority die | recessive scaf11 scaff01332 scafPBss viable, with Scer\GAL4pnr-MD237 scafGD5263 Other Phenotypes Allele visible | recessive scaf11 scaff01332 scafPBss Phenotype manifest in Allele adult head scafPBss scaff01332 scaf11 embryonic/first instar larval cuticle scaf11 scaf27
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of scaf Class Mutagen Stocks Known lesion scafc04529 piggyBac transposase 1 -- scaff01332 piggyBac transposase 1 -- scafKG05129 P-element activity 1 -- scafMI03824 1 -- scaf11 P-element activity 0 Yes scaf27 P-element activity 0 Yes scafPBss piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of scaf Class Mutagen Stocks Known lesion scafGD5263 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes scafJF03318 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes scafNIG.11066R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes scafScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)nap11 (Rousset et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.11066R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}scafMI03824 NA P{SUPor-P}scafKG05129 NA PBac{PB}scafc04529 NA PBac{PBss}scafPBss NA PBac{WH}scaff01332 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
imaginal disc-derived male genitalia morphogenesis inferred from mutant phenotype (Rousset et al., 2010)
Cellular Component
CV term Evidence References
extracellular space inferred from direct assay (Rousset et al., 2010)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
NOT serine-type endopeptidase activity non-traceable author statement (Ross et al., 2003) serine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
proteolysis inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
scaf - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) scaf - CG6724 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions scaf allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033033 DroID - A comprehensive database of gene and protein interactions. • FBgn0033033
Orthologs
OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6X3HM0 Dipteran inclusive ortholog search EOG6M39HT Insect inclusive ortholog search EOG60GBMN Arthropod inclusive ortholog search EOG6RJDH9 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6X3HM0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\scaf     Drosophila simulans   Dsim\GD10353 Y   Drosophila sechellia   Dsec\GM16503 Y   Drosophila erecta   Dere\GG10851 Y   Drosophila yakuba   Dyak\GE19444 Y   Drosophila ananassae   Dana\GF11026 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10737 Y   Drosophila persimilis   Dper\GL11062 Y   Drosophila willistoni   Dwil\GK10763 Y   Drosophila virilis   Dvir\GJ22229 Y   Drosophila mojavensis   Dmoj\GI20401 Y   Drosophila grimshawi   Dgri\GH21274 Y
Orthologs in non-Drosophila Dipterans (EOG6M39HT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014650   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008589   Anopheles gambiae Malaria mosquito  Agam\CLIPE1   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001726
Orthologs in non-Dipteran Insects (EOG60GBMN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50290   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012989   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12077   Atta cephalotes Leafcutter ant  Acep\ACEP10112   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13207   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21539   Linepithema humile Argentine ant  Lhum\LH26237   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24519   Solenopsis invicta Red fire ant  Sinv\SINV18504   Bombyx mori Silkmoth  Bmor\BGIBMGA011362   Pediculus humanus Human body louse  Phum\PHUM569140   Tribolium castaneum Red flour beetle  Tcas\H164
Orthologs in non-Insect Arthropods (EOG6RJDH9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_313807
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10353 Drosophila sechellia Dsec\GM16503 Drosophila erecta Dere\GG10851 Drosophila yakuba Dyak\GE19444 Drosophila ananassae Dana\GF11026 Drosophila pseudoobscura pseudoobscura Dpse\GA10737 Drosophila persimilis Dper\GL11062 Drosophila willistoni Dwil\GK10763 Drosophila virilis Dvir\GJ22229 Drosophila mojavensis Dmoj\GI20401 Drosophila grimshawi Dgri\GH21274
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	29386 y1 v1; P{TRiP.JF03318}attP2 36974 y1 w*; Mi{MIC}scafMI03824/SM6a 13631 y1 w67c23; P{SUPor-P}scafKG05129
Harvard	- PBac{PB}scafc04529 - PBac{WH}scaff01332
VDRC	v13249 w1118; P{GD5263}v13249
Genomic Clones ( 2 )
	BACR27C13 BACR45O18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 101 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05918
Other clones	DPiM_GH05918
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL01106 LP02349 LP03429 LP07961 RE30179 RH39275 RH43136 RH48169 RH48285
Other clones	12294 15682 30272 31856 38159 39308 41449 41567 47537 52647 57818 57931 61980 63438 102493 105261 118648 144428 145744 148460 148552 163418 172762 175039 175211 194560 273147 280457 280471 283543 bs57h06 CK01.6C.F8 CK01.12A.H3 CK01.69C.C11 CK01.119A.A8 CK01.125D.A10 CK01.126A.E3 CK01.127A.F5 CK01.144C.G8 EK050403 EK065610 EK090836 EK096533 EK097323 EK114320 EK139317 EK142549 EK152412 EK154747 EK154833 EK165442 EK168857 EK174021 EK175536 EK178648 EK180464 EK185522 EK189224 EK230558 EK250857 EK254330 EK254339 EK267833 EK272655 EK299953 EK305354 EK305410 ESG017d.E21_F10 ESG0120a.B21_C05 RP001011898 RP001012296 RP001016660 RP001018140 RP001018428 RP001019022 RP001020074 RP001023250 RP001034875 RP001040825 RP001043753 RP001043969 RP001049068 RP001049140 RP001050422 RP001050515 RP001050842 RP001053420 RP001055948 RP001056249 SD06537
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033033 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35505
Antibody Information
	polyclonal (Rousset et al., 2010, Sorrosal et al., 2010)
Other Information
Discoverer
Etymology
	The gene is named "scarface" based on the phenotype of homozygous adult escapers, which have what appears to be "scarring" around the mouthparts. (Bonin and Mann, 2004)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: scaf scarface (Rousset et al., 2010) Source for identity of: scarface CG11066 (Bonin and Mann, 2004)
Source for database merge of	Source for merge of: CG11066 CG15900 (Whitfield, 2000.12.15)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136336 NM_165441 RefSeq (Proteins) • NP_610180 NP_724424 DDBJ / EMBL / GenBank • AAF57320 AAK77280 AAM68354 AI107997 AI517939 AY047548 BH759575 CZ469325 CZ483805 CZ483806 Entrez Gene - A searchable database of RefSeq genes. • 35505 UniProtKB/TrEMBL • Q7K5M0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH05918
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033033 DroID - A comprehensive database of gene and protein interactions. • FBgn0033033 DRSC - Results from RNAi screens. • FBgn0033033 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033033 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11066-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033033 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35505 modMine - Data generated by the modENCODE project. • FBgn0033033
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG11066 (Bonin and Mann, 2004, Corradini et al., 2003, Myster et al., 2004, Collett, 2008.2.16, Perkins et al., 2009, Kim et al., 2005) CG15900 (Boutros et al., 2002, ) scaf (Rousset et al., 2010, Lloret-Llinares et al., 2012) scarf (Sorrosal et al., 2010) scarface (Japanese National Institute of Genetics, 2012.5.21) SPH142 (Ross et al., 2003)
Name Synonym	scarface (Bonin and Mann, 2004, Collett, 2008.2.16, Chen et al., 2011, Yakoby et al., 2008, Gilchrist et al., 2008, Rousset et al., 2010) Scarface (Sorrosal et al., 2010)
Secondary FlyBase IDs
	FBgn0033034
References ( 30 )
Generate a list of	All references relating to this gene ( 30 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 2 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Lloret-Llinares et al., 2012, Nucleic Acids Res. 40(19): 9493--9505 dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes. [FBrf0219748] Chen et al., 2011, J. Biol. Chem. 286(11): 9020--9030 E2 Ligase dRad6 Regulates DMP53 Turnover in Drosophila. [FBrf0213391] Show all research papers ...