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Gene Dmel\geminin

General Information
Symbol	Dmel\geminin	Species	D. melanogaster
Name	geminin	Annotation symbol	CG3183
Feature type	protein_coding_gene	FlyBase ID	FBgn0033081
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	Gem, Dm Geminin, v(2)k09107, l(2)k14019
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42C2-42C2	Sequence location	2R:2,542,968..2,543,955 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene geminin is referred to in FlyBase by the symbol Dmel\geminin (CG3183, FBgn0033081). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: negative regulation of epidermal growth factor receptor signaling pathway; endomitotic cell cycle; negative regulation of DNA replication. 11 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; non-connected developing system; organ system; cell cycle; cell cycle process; imaginal precursor; external compound sense organ; embryonic hindgut; organ system subdivision; portion of tissue. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Geminin family. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, inseminated spermatheca, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1623108_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of geminin. Gene sequence location is 2R:2542968..2543955.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42C2-42C2
Experimentally Determined Cytological Location
Cytogenetic map Notes References 42C1-42C2   (determined by in situ hybridisation)   (Spradling et al., 1999) 42C1-42C2   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\geminin for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) geminin-RA FBtr0086147  NM_136374   988   192 geminin-RB FBtr0336665  NM_001273798   939   192
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein geminin-PA   FBpp0085480   21.4   192   4.26     NP_610218   AAF57366 geminin-PB   FBpp0307646   21.4   192   4.26     NP_001260727   AGB93260
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Geminin family (IPR022786)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201700   AA264279   AA696592   AA802909   AA802911   AA941368   AE013599 AAF57366   AF407275 AAK96219   AE013599 AGB93260   AI513174   AI944925   AQ025885   AQ034034   AX482633   AY095203 AAM12296   AY231771   BG632679   BI141900   BI167348   BI167697   BI172792   BI173606   BI214153   BI216147   BI236048   BI242410   BI354725   BI363892   BI365225   BI365450   BI374000   BI483349   BI484753   BI486165   BI632416   BI636039   BI641067   BP541429   BP551948   BP551949   CK660872   CO179805   CO263201   CO265747   CO278347   CO298283   CO306817   CO312965   CO320289   CO325375   CO338496   DN153953   EC059254   EC209853   EC210011   EC221356   EC221756   EC225547   EC235362   EC239176   EC242661   EC249021   EC266232   EL882039   EL882040   EL882167   EL882168   EV584835   GH849315
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JX41
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic main segment of Malpighian tubule   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic/larval lymph gland   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) mushroom body primordium   (Fisher et al., 2012) Comment:reported as embryonic central brain mushroom body sensory nervous system primordium   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   wing disc   (Quinn et al., 2004) Comment:G2 phase of mitotic cell cycle
Additional Descriptive Data
	geminin protein is present in late S phase and G2 cells, but absent from G1-arrested cells. Expression is excluded from the ZNC, a zone of cells arrested in G1 or G2, along the dorsal/ventral compartment boundary in the wing disc. (Quinn et al., 2004)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Quinn et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, inseminated spermatheca, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary    1316.3 Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (738.3) Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary  (1316.3) Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary  (1316.3) Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary    1316.3 Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary    1316.3 Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary  (1316.3) Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary  1316.3 Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    738.3 Larval Midgut    90.1 Larval Hindgut    161.1 Larval Malpighian Tubules    120.1 Larval Fat Body    99.9 Larval Salivary Gland    360.3 Larval Trachea    322.875 Larval Carcass    157.425 Adult Head    129.4 Adult Eye    104.75 Adult Brain    166.3 Adult Thoracic-Abdominal Ganglion    130.2 Adult Crop    119.7 Adult Midgut    156.7 Adult Hindgut    113.5 Adult Malpighian Tubules    106.5 Adult Fat Body    64.1 Adult Salivary Gland    142.1 Adult Heart    87.65 Adult VirginFemale Spermatheca    80.3 Adult InseminatedFemale Spermatheca    104.2 Adult Ovary    1316.3 Adult Testis    203.1 Adult Male Accessory Gland    206.4 Adult Carcass    100.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    129.4   NA  Eye    104.75   NA  Brain    166.3   738.3  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    130.2   NA  Crop    119.7   90.1  Midgut    156.7   161.1  Hindgut    113.5   120.1  Malpighian Tubules    106.5   99.9  Fat Body    64.1   360.3  Salivary Gland    142.1   NA  Heart    87.65   322.875  Trachea    NA   NA  VirginFemale Spermatheca    80.3   NA  InseminatedFemale Spermatheca    104.2   NA  Ovary    1316.3   NA  Testis    203.1   NA  Male Accessory Gland    206.4   157.425  Carcass    100.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    146 central nervous system, larvae L3    122 central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female    369 ovary, mated 4-day female    454 testis, mated 4-day male    107 accessory gland, mated 4-day male    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (146) central nervous system, larvae L3  (122) central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male  (28) head, mated 20-day male  (32) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering  (36) digestive system, 1-day adult  (27) digestive system, 4-day adult  (45) digestive system, 20-day adult  (32) fat body, larvae L3 wandering    7 fat body, white prepupae  (27) fat body, pupae P8  (31) carcass, larvae L3 wandering  (83) carcass, 1-day adult    20 carcass, 4-day adult  (39) carcass, 20-day adult  (28) ovary, virgin 4-day female  (369) ovary, mated 4-day female  (454) testis, mated 4-day male  (107) accessory gland, mated 4-day male  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (146) central nervous system, larvae L3  (122) central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female  (369) ovary, mated 4-day female  (454) testis, mated 4-day male  107 accessory gland, mated 4-day male    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    146 central nervous system, larvae L3    122 central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female    369 ovary, mated 4-day female    454 testis, mated 4-day male    107 accessory gland, mated 4-day male    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    146 central nervous system, larvae L3    122 central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female    369 ovary, mated 4-day female    454 testis, mated 4-day male    107 accessory gland, mated 4-day male    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (146) central nervous system, larvae L3  (122) central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male  28 head, mated 20-day male  32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering  (36) digestive system, 1-day adult  27 digestive system, 4-day adult  (45) digestive system, 20-day adult  32 fat body, larvae L3 wandering    7 fat body, white prepupae  27 fat body, pupae P8  31 carcass, larvae L3 wandering  (83) carcass, 1-day adult    20 carcass, 4-day adult  (39) carcass, 20-day adult  28 ovary, virgin 4-day female  (369) ovary, mated 4-day female  (454) testis, mated 4-day male  (107) accessory gland, mated 4-day male  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (146) central nervous system, larvae L3  122 central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female  (369) ovary, mated 4-day female  (454) testis, mated 4-day male  107 accessory gland, mated 4-day male    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    146 central nervous system, larvae L3    122 central nervous system, pupae P8    25 head, virgin 1-day female    13 head, virgin 4-day female    18 head, virgin 20-day female    10 head, mated 1-day female    17 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    18 head, mated 4-day male    28 head, mated 20-day male    32 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    36 digestive system, 1-day adult    27 digestive system, 4-day adult    45 digestive system, 20-day adult    32 fat body, larvae L3 wandering    7 fat body, white prepupae    27 fat body, pupae P8    31 carcass, larvae L3 wandering    83 carcass, 1-day adult    20 carcass, 4-day adult    39 carcass, 20-day adult    28 ovary, virgin 4-day female    369 ovary, mated 4-day female    454 testis, mated 4-day male    107 accessory gland, mated 4-day male    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    331 embryo 02-04hr    383 embryo 04-06hr    344 embryo 06-08hr    360 embryo 08-10hr    265 embryo 10-12hr    143 embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr    135 embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day    125 adult female 30day    133 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (331) embryo 02-04hr  (383) embryo 04-06hr  (344) embryo 06-08hr  (360) embryo 08-10hr  (265) embryo 10-12hr  (143) embryo 12-14hr  (98) embryo 14-16hr  (90) embryo 16-18hr  (68) embryo 18-20hr  (42) embryo 20-22hr  (135) embryo 22-24hr  (93) larva L1  26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2  26 larva L3 puffstage 3-6  (37) larva L3 puffstage 7-9  (37) white prepupae new  (38) white prepupae 12hr  (47) white prepupae 24hr  (55) pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day  26 adult male 30day    25 adult female 01day  (59) adult female 05day  (125) adult female 30day  (133) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (331) embryo 02-04hr  (383) embryo 04-06hr  (344) embryo 06-08hr  (360) embryo 08-10hr  (265) embryo 10-12hr  (143) embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr  (135) embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day  (125) adult female 30day  (133) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    331 embryo 02-04hr    383 embryo 04-06hr    344 embryo 06-08hr    360 embryo 08-10hr    265 embryo 10-12hr    143 embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr    135 embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day    125 adult female 30day    133 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    331 embryo 02-04hr    383 embryo 04-06hr    344 embryo 06-08hr    360 embryo 08-10hr    265 embryo 10-12hr    143 embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr    135 embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day    125 adult female 30day    133 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (331) embryo 02-04hr  (383) embryo 04-06hr  (344) embryo 06-08hr  (360) embryo 08-10hr  (265) embryo 10-12hr  (143) embryo 12-14hr  (98) embryo 14-16hr  (90) embryo 16-18hr  (68) embryo 18-20hr  (42) embryo 20-22hr  (135) embryo 22-24hr  (93) larva L1  26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2  26 larva L3 puffstage 3-6  (37) larva L3 puffstage 7-9  (37) white prepupae new  (38) white prepupae 12hr  (47) white prepupae 24hr  (55) pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day  26 adult male 30day    25 adult female 01day  (59) adult female 05day  (125) adult female 30day  (133) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (331) embryo 02-04hr  (383) embryo 04-06hr  (344) embryo 06-08hr  (360) embryo 08-10hr  (265) embryo 10-12hr  143 embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr  135 embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day  125 adult female 30day  133 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    331 embryo 02-04hr    383 embryo 04-06hr    344 embryo 06-08hr    360 embryo 08-10hr    265 embryo 10-12hr    143 embryo 12-14hr    98 embryo 14-16hr    90 embryo 16-18hr    68 embryo 18-20hr    42 embryo 20-22hr    135 embryo 22-24hr    93 larva L1    26 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    37 white prepupae new    38 white prepupae 12hr    47 white prepupae 24hr    55 pupae 2d postWPP    25 pupae 3d postWPP    16 pupae 4d postWPP    18 adult male 01day    19 adult male 05day    26 adult male 30day    25 adult female 01day    59 adult female 05day    125 adult female 30day    133 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    309 Schneider line 2 Sg4    137 embryonic 1182-4H    167 embryonic GM2    102 embryonic Kc167    101 embryonic S1    254 embryonic S3    154 leg disc CME L1    269 wing disc CME-W2    80 wing disc ML-DmD8    132 wing disc ML-DmD9    210 wing disc ML-DmD16-c3    438 wing disc ML-DmD21    209 wing disc ML-DmD32    214 haltere disc ML-DmD17-c3    167 eye-antennal disc ML-DmD11    148 antennal disc ML-DmD20-c5    164 mixed discs ML-DmD4-c1    237 CNS ML-DmBG1-c1    358 CNS ML-DmBG2-c2    112 tumorous blood cells mbn2    104 ovary fGS/OSS    548 ovary OSC    346 ovary OSS    261 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (309) Schneider line 2 Sg4  (137) embryonic 1182-4H  (167) embryonic GM2  (102) embryonic Kc167  (101) embryonic S1  (254) embryonic S3  (154) leg disc CME L1  (269) wing disc CME-W2  (80) wing disc ML-DmD8  (132) wing disc ML-DmD9  (210) wing disc ML-DmD16-c3  (438) wing disc ML-DmD21  (209) wing disc ML-DmD32  (214) haltere disc ML-DmD17-c3  (167) eye-antennal disc ML-DmD11  (148) antennal disc ML-DmD20-c5  (164) mixed discs ML-DmD4-c1  (237) CNS ML-DmBG1-c1  (358) CNS ML-DmBG2-c2  (112) tumorous blood cells mbn2  (104) ovary fGS/OSS  (548) ovary OSC  (346) ovary OSS  (261) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (309) Schneider line 2 Sg4  (137) embryonic 1182-4H  (167) embryonic GM2  102 embryonic Kc167  101 embryonic S1  (254) embryonic S3  (154) leg disc CME L1  (269) wing disc CME-W2    80 wing disc ML-DmD8  (132) wing disc ML-DmD9  (210) wing disc ML-DmD16-c3  (438) wing disc ML-DmD21  (209) wing disc ML-DmD32  (214) haltere disc ML-DmD17-c3  (167) eye-antennal disc ML-DmD11  (148) antennal disc ML-DmD20-c5  (164) mixed discs ML-DmD4-c1  (237) CNS ML-DmBG1-c1  (358) CNS ML-DmBG2-c2  (112) tumorous blood cells mbn2  104 ovary fGS/OSS  (548) ovary OSC  (346) ovary OSS  (261) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    309 Schneider line 2 Sg4    137 embryonic 1182-4H    167 embryonic GM2    102 embryonic Kc167    101 embryonic S1    254 embryonic S3    154 leg disc CME L1    269 wing disc CME-W2    80 wing disc ML-DmD8    132 wing disc ML-DmD9    210 wing disc ML-DmD16-c3    438 wing disc ML-DmD21    209 wing disc ML-DmD32    214 haltere disc ML-DmD17-c3    167 eye-antennal disc ML-DmD11    148 antennal disc ML-DmD20-c5    164 mixed discs ML-DmD4-c1    237 CNS ML-DmBG1-c1    358 CNS ML-DmBG2-c2    112 tumorous blood cells mbn2    104 ovary fGS/OSS    548 ovary OSC    346 ovary OSS    261 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    309 Schneider line 2 Sg4    137 embryonic 1182-4H    167 embryonic GM2    102 embryonic Kc167    101 embryonic S1    254 embryonic S3    154 leg disc CME L1    269 wing disc CME-W2    80 wing disc ML-DmD8    132 wing disc ML-DmD9    210 wing disc ML-DmD16-c3    438 wing disc ML-DmD21    209 wing disc ML-DmD32    214 haltere disc ML-DmD17-c3    167 eye-antennal disc ML-DmD11    148 antennal disc ML-DmD20-c5    164 mixed discs ML-DmD4-c1    237 CNS ML-DmBG1-c1    358 CNS ML-DmBG2-c2    112 tumorous blood cells mbn2    104 ovary fGS/OSS    548 ovary OSC    346 ovary OSS    261 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (309) Schneider line 2 Sg4  (137) embryonic 1182-4H  (167) embryonic GM2  (102) embryonic Kc167  (101) embryonic S1  (254) embryonic S3  (154) leg disc CME L1  (269) wing disc CME-W2  (80) wing disc ML-DmD8  (132) wing disc ML-DmD9  (210) wing disc ML-DmD16-c3  (438) wing disc ML-DmD21  (209) wing disc ML-DmD32  (214) haltere disc ML-DmD17-c3  (167) eye-antennal disc ML-DmD11  (148) antennal disc ML-DmD20-c5  (164) mixed discs ML-DmD4-c1  (237) CNS ML-DmBG1-c1  (358) CNS ML-DmBG2-c2  (112) tumorous blood cells mbn2  (104) ovary fGS/OSS  (548) ovary OSC  (346) ovary OSS  (261) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (309) Schneider line 2 Sg4  137 embryonic 1182-4H  (167) embryonic GM2  102 embryonic Kc167  101 embryonic S1  (254) embryonic S3  (154) leg disc CME L1  (269) wing disc CME-W2    80 wing disc ML-DmD8  132 wing disc ML-DmD9  (210) wing disc ML-DmD16-c3  (438) wing disc ML-DmD21  (209) wing disc ML-DmD32  (214) haltere disc ML-DmD17-c3  (167) eye-antennal disc ML-DmD11  (148) antennal disc ML-DmD20-c5  (164) mixed discs ML-DmD4-c1  (237) CNS ML-DmBG1-c1  (358) CNS ML-DmBG2-c2  112 tumorous blood cells mbn2  104 ovary fGS/OSS  (548) ovary OSC  (346) ovary OSS  (261) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    309 Schneider line 2 Sg4    137 embryonic 1182-4H    167 embryonic GM2    102 embryonic Kc167    101 embryonic S1    254 embryonic S3    154 leg disc CME L1    269 wing disc CME-W2    80 wing disc ML-DmD8    132 wing disc ML-DmD9    210 wing disc ML-DmD16-c3    438 wing disc ML-DmD21    209 wing disc ML-DmD32    214 haltere disc ML-DmD17-c3    167 eye-antennal disc ML-DmD11    148 antennal disc ML-DmD20-c5    164 mixed discs ML-DmD4-c1    237 CNS ML-DmBG1-c1    358 CNS ML-DmBG2-c2    112 tumorous blood cells mbn2    104 ovary fGS/OSS    548 ovary OSC    346 ovary OSS    261 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    146 cold shock, 4-day adult    92 heat shock, 4-day adult    124 Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    127 Cadmium 100 mM 48 hrs, 4-day adult    290 Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult    177 Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3    239 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    277 Caffeine 25 mg/ml 48 hrs, 4-day adult    168 Paraquat 5 mM 48 hrs, 4-day adult    195 Paraquat 10 mM 48 hrs, 4-day adult    163 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (146) cold shock, 4-day adult  (92) heat shock, 4-day adult  (124) Cadmium 50 mM 6 hrs, larvae L3  (38) Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult  (127) Cadmium 100 mM 48 hrs, 4-day adult  (290) Copper 0.5 mM 12 hrs, larvae L3  (56) Copper 15 mM 48 hrs, 4-day adult  (177) Zinc 5 mM 12 hrs, larvae L3  (61) Zinc 4.5 mM 48 hrs, 4-day adult  (90) Ethanol 2.5% 3 hrs, larvae L3  (239) Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3  (37) Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (277) Caffeine 25 mg/ml 48 hrs, 4-day adult  (168) Paraquat 5 mM 48 hrs, 4-day adult  (195) Paraquat 10 mM 48 hrs, 4-day adult  (163) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (42) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (146) cold shock, 4-day adult    92 heat shock, 4-day adult  (124) Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult  (127) Cadmium 100 mM 48 hrs, 4-day adult  (290) Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult  (177) Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3  (239) Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (277) Caffeine 25 mg/ml 48 hrs, 4-day adult  (168) Paraquat 5 mM 48 hrs, 4-day adult  (195) Paraquat 10 mM 48 hrs, 4-day adult  (163) Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    146 cold shock, 4-day adult    92 heat shock, 4-day adult    124 Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    127 Cadmium 100 mM 48 hrs, 4-day adult    290 Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult    177 Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3    239 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    277 Caffeine 25 mg/ml 48 hrs, 4-day adult    168 Paraquat 5 mM 48 hrs, 4-day adult    195 Paraquat 10 mM 48 hrs, 4-day adult    163 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    146 cold shock, 4-day adult    92 heat shock, 4-day adult    124 Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    127 Cadmium 100 mM 48 hrs, 4-day adult    290 Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult    177 Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3    239 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    277 Caffeine 25 mg/ml 48 hrs, 4-day adult    168 Paraquat 5 mM 48 hrs, 4-day adult    195 Paraquat 10 mM 48 hrs, 4-day adult    163 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (146) cold shock, 4-day adult  (92) heat shock, 4-day adult  (124) Cadmium 50 mM 6 hrs, larvae L3  (38) Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult  (127) Cadmium 100 mM 48 hrs, 4-day adult  (290) Copper 0.5 mM 12 hrs, larvae L3  (56) Copper 15 mM 48 hrs, 4-day adult  (177) Zinc 5 mM 12 hrs, larvae L3  (61) Zinc 4.5 mM 48 hrs, 4-day adult  (90) Ethanol 2.5% 3 hrs, larvae L3  (239) Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3  (37) Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (277) Caffeine 25 mg/ml 48 hrs, 4-day adult  (168) Paraquat 5 mM 48 hrs, 4-day adult  (195) Paraquat 10 mM 48 hrs, 4-day adult  (163) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (42) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (146) cold shock, 4-day adult    92 heat shock, 4-day adult  124 Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult  127 Cadmium 100 mM 48 hrs, 4-day adult  (290) Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult  (177) Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3  (239) Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (277) Caffeine 25 mg/ml 48 hrs, 4-day adult  (168) Paraquat 5 mM 48 hrs, 4-day adult  (195) Paraquat 10 mM 48 hrs, 4-day adult  (163) Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    146 cold shock, 4-day adult    92 heat shock, 4-day adult    124 Cadmium 50 mM 6 hrs, larvae L3    38 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    127 Cadmium 100 mM 48 hrs, 4-day adult    290 Copper 0.5 mM 12 hrs, larvae L3    56 Copper 15 mM 48 hrs, 4-day adult    177 Zinc 5 mM 12 hrs, larvae L3    61 Zinc 4.5 mM 48 hrs, 4-day adult    90 Ethanol 2.5% 3 hrs, larvae L3    239 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    37 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    277 Caffeine 25 mg/ml 48 hrs, 4-day adult    168 Paraquat 5 mM 48 hrs, 4-day adult    195 Paraquat 10 mM 48 hrs, 4-day adult    163 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • RE66227 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033081 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3183-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for geminin Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2R)ST1) geminink03202b geminink14019 lethal | P-stage, with Scer\GAL4en-e16E geminindsRNA.Scer\UAS.cHa lethal | recessive geminink03202b geminink14019 Sterility Allele female sterile | partially geminink09107 Other Phenotypes Allele endomitotic cell cycle defective, with Scer\GAL4Act5C.PP gemininScer\UAS.cQa increased cell death, with Scer\GAL4en-e16E gemininScer\UAS.cQa mitotic cell cycle defective | third instar larval stage, with Scer\GAL4hs.PB gemininScer\UAS.cQa visible, with Scer\GAL4ey.PH gemininScer\UAS.cQa visible, with Scer\GAL4GMR.PF gemininScer\UAS.cQa visible | recessive geminink09107 Phenotype manifest in Allele anaphase & condensed nuclear chromosome (with Df(2R)ST1) geminink03202b cycle 16 embryo (with Df(2R)ST1) geminink03202b embryonic/larval midgut primordium (with Df(2R)ST1) geminink03202b embryonic/larval neuron | ectopic, with Scer\GAL4en-e16E gemininScer\UAS.cQa embryonic hindgut (with Df(2R)ST1) geminink03202b embryonic Malpighian tubule (with Df(2R)ST1) geminink03202b eye, with Scer\GAL4ey.PH gemininScer\UAS.cQa eye, with Scer\GAL4GMR.PF gemininScer\UAS.cQa eye disc, with Scer\GAL4ey.PH gemininScer\UAS.cQa eye disc, with Scer\GAL4GMR.PF gemininScer\UAS.cQa follicle cell (with geminink09107) geminink14019 follicle cell (with geminink14019) geminink09107 imaginal disc geminink14019 lch5 neuron, with Scer\GAL4h-1J3 gemininScer\UAS.cQa mitotic anaphase (with Df(2R)ST1) geminink03202b mitotic cell cycle (with Df(2R)ST1) geminink03202b M phase of mitotic cell cycle, with Scer\GAL4en-e16E gemininScer\UAS.cQa ommatidium, with Scer\GAL4GMR.PF gemininScer\UAS.cQa peripheral nervous system | ectopic, with Scer\GAL4en-e16E gemininScer\UAS.cQa peripheral nervous system | ectopic, with Scer\GAL4h-1J3 gemininScer\UAS.cQa presumptive embryonic/larval central nervous system (with Df(2R)ST1) geminink03202b presumptive embryonic/larval peripheral nervous system, with Scer\GAL4en-e16E gemininScer\UAS.cQa S phase, with Scer\GAL4en-e16E gemininScer\UAS.cQa S phase, with Scer\GAL4ey.PH gemininScer\UAS.cQa S phase, with Scer\GAL4GMR.PF gemininScer\UAS.cQa wing disc | third instar larval stage, with Scer\GAL4hs.PB gemininScer\UAS.cQa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of geminin Class Mutagen Stocks Known lesion geminink14019 P-element activity 3 Yes geminink03202b P-element activity 1 Yes geminink09107 P-element activity 1 Yes gemininUM-8107-3 1 -- gemininunspecified 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of geminin Class Mutagen Stocks Known lesion geminindsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes gemininGL01299 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes gemininGLV21038 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes geminindsRNA.Scer\UAS.cHa in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes gemininGD4453 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes gemininScer\UAS.cQa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)cn88b (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)hfl-37 (Rendina et al., 2010) Df(2R)nap2 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)nap16 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)ST1 (Quinn et al., 2001)
Not disrupted in	Df(2R)42 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4453} NA P{TRiP.GL01299} NA P{TRiP.GLV21038} NA P{UAS-geminin.dsRNA} NA P{UAS-geminin.Q} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}geminink03202b No P{lacW}geminink09107 No P{lacW}geminink14019 No miscellaneous insertions P{RS3}gemininUM-8107-3 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
endomitotic cell cycle inferred from mutant phenotype (Narbonne-Reveau et al., 2008) negative regulation of DNA replication inferred from direct assay (Hall et al., 2008) inferred from direct assay AND inferred from mutant phenotype (Quinn et al., 2001) negative regulation of epidermal growth factor receptor signaling pathway inferred from genetic interaction with brm (Herr et al., 2010)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Quinn et al., 2001)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Quinn, 2001.8.7)
Biological Process
CV term Evidence References
eggshell chorion gene amplification traceable author statement (Lee and Orr-Weaver, 2003) negative regulation of DNA replication non-traceable author statement (Quinn, 2001.8.7) traceable author statement (Lee and Orr-Weaver, 2003) regulation of cell cycle inferred from sequence or structural similarity (O'Farrell, 2001.4.12)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
geminin - CG10158 experimental knowledge based (Guruharsha et al., 2011) geminin - CG17494 experimental knowledge based (Guruharsha et al., 2011) geminin - Cen experimental knowledge based (Guruharsha et al., 2011) geminin - RluA-2 experimental knowledge based (Guruharsha et al., 2011) geminin - Ubqn experimental knowledge based (Guruharsha et al., 2011) geminin - ana2 experimental knowledge based (Guruharsha et al., 2011) geminin - cmet experimental knowledge based (Guruharsha et al., 2011) geminin - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) geminin - dup experimental knowledge based (Guruharsha et al., 2011) geminin - mub experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) geminin - nxf2 experimental knowledge based (Guruharsha et al., 2011) geminin - row experimental knowledge based (Guruharsha et al., 2011) geminin - tlk experimental knowledge based (Guruharsha et al., 2011) geminin - woc experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions geminin allele Gene References gemininScer\UAS.cQa dup (Quinn et al., 2001) gemininunspecified dup (Quinn et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61462 DroID - A comprehensive database of gene and protein interactions. • FBgn0033081 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35563
Orthologs
OrthoDB Orthologs (53) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HT9CQ Dipteran inclusive ortholog search EOG6TQMK8 Insect inclusive ortholog search EOG6F4R7T Arthropod inclusive ortholog search EOG6905SQ Metazoa inclusive ortholog search EOG6JX78F
Orthologs in Drosophila Species (EOG6HT9CQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\geminin     Drosophila simulans   Dsim\GD10317 Y   Drosophila sechellia   Dsec\GM20866 Y   Drosophila erecta   Dere\GG10817 Y   Drosophila yakuba   Dyak\geminin Y   Drosophila ananassae   Dana\GF13805 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16512 Y   Drosophila persimilis   Dper\GL10217 Y   Drosophila willistoni   Dwil\GK19620 Y   Drosophila virilis   Dvir\GJ20704 Y   Drosophila mojavensis   Dmoj\GI20984 Y   Drosophila grimshawi   Dgri\GH20873 Y
Orthologs in non-Drosophila Dipterans (EOG6TQMK8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007474   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009773   Anopheles gambiae Malaria mosquito  Agam\AGAP000496   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014376   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013106   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003563   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005150   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ018199
Orthologs in non-Dipteran Insects (EOG6F4R7T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB51526   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG026752   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004042   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15031   Atta cephalotes Leafcutter ant  Acep\ACEP25583   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20546   Camponotus floridanus Florida carpenter ant  Cflo\CFLO11208   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15326   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23393   Linepithema humile Argentine ant  Lhum\LH19694   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23230   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11911   Solenopsis invicta Red fire ant  Sinv\SINV20020   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003994   Acyrthosiphon pisum Pea aphid  Apim\ACYPI55631   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008609   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003498   Bombyx mori Silkmoth  Bmor\BGIBMGA000660   Pediculus humanus Human body louse  Phum\PHUM365140   Tribolium castaneum Red flour beetle  Tcas\TC006282
Orthologs in non-Insect Arthropods (EOG6905SQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW020190
Orthologs in non-Arthropod Metazoa (EOG6JX78F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005762gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023715gn   Danio rerio Zebrafish  Drer\gmnn   Xenopus tropicalis Western clawed frog  Xtro\gmnn   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033274   Gallus gallus Domestic chicken  Ggal\GMNN   Mus musculus House mouse  Mmus\Idas   Mus musculus House mouse  Mmus\Gmnn   Rattus norvegicus Norway rat  Rnor\Gmnn   Rattus norvegicus Norway rat  Rnor\LOC688802   Homo sapiens Human  Hsap\GMNN   Homo sapiens Human  Hsap\ENSG00000234602
Human Orthologs (2)
Gene OMIM HGNC Hsap\GMNN     Hsap\ENSG00000234602
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10317 Drosophila sechellia Dsec\GM20866 Drosophila erecta Dere\GG10817 Drosophila yakuba Dyak\geminin Drosophila ananassae Dana\GF13805 Drosophila pseudoobscura pseudoobscura Dpse\GA16512 Drosophila persimilis Dper\GL10217 Drosophila willistoni Dwil\GK19620 Drosophila virilis Dvir\GJ20704 Drosophila mojavensis Dmoj\GI20984 Drosophila grimshawi Dgri\GH20873
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	30929 w*; P{UAS-geminin.RNAi}M4 35673 y1 sc* v1; P{TRiP.GLV21038}attP2 41869 y1 sc* v1; P{TRiP.GL01299}attP40 11118 y1 w67c23; P{lacW}geminink14019/CyO 10860 y1 w67c23; P{lacW}geminink09107, l(2)k09107k09107/CyO
Kyoto	124345 w1118; P{RS3}gemininUM-8107-3 108863 y1 w67c23; P{lacW}geminink14019/CyO 102909 y1 w67c23 ; P{lacW}geminink14019 / CyO 102263 y1 w67c23 ; P{lacW}geminink03202b / CyO
Genomic Clones ( 1 )
	BACR01C10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 57 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE66227
Other clones	yak-em_CG3183
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH12277 GM06552 GM07965 GM28064 LD04903 LD07974 LD25484 LD45374 LP19810 RE07715 RE08138 RE15467 RE16558 RE19870 RE23078 RE32037 RE39733 RE48867 RE50467 RE50767 RE61490 RE67904 RE69548 SD15868 SD17604 SD24175 SD26180
Other clones	17513 31137 31154 33130 67743 105605 122120 178609 572732 bs07a09 CK01.104D.D4 Dmel_dig_010602_0630_1060 EC00238 EK087746 EK129833 EK140150 EK143539 EK172663 EK196338 EK230824 EK252460 EK283624 EN16940 FGC001E09 FGM215G06 IP11839 IP11939 RP003671357
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033081 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35563
Antibody Information
	polyclonal antibody (Quinn et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: geminin CG3183 (Quinn et al., 2001, FlyBase, 1996-)
Source for database merge of	Source for merge of: geminin anon-WO0257455.1 (FlyBase, 1996-) Source for merge of: geminin l(2)k14019 v(2)k09107 (Quinn et al., 2001)
Additional comments	Source for merge of geminin anon-WO0257455.1 was sequence comparison (date:051113). (FlyBase, 1996-) Source for identity of geminin CG3183 was sequence comparison (date:010908). (FlyBase, 1996-)
Other Comments
	RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) geminin has roles in limiting DNA replication, in anaphase and in neural differentiation. (Quinn et al., 2001) Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology. (Roch et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136374 NM_001273798 RefSeq (Proteins) • NP_610218 NP_001260727 DDBJ / EMBL / GenBank • AA201700 AA264279 AA802911 AAF57366 AAK96219 AAM12296 AF407275 AGB93260 AI513174 AI944925 AQ025885 AQ034034 AX482633 AY095203 BI483349 Entrez Gene - A searchable database of RefSeq genes. • 35563 UniProtKB/TrEMBL • Q7JX41
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Geminin family (IPR022786) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE66227
Linkouts
	BioGRID - A database of protein and genetic interactions • 61462 DroID - A comprehensive database of gene and protein interactions. • FBgn0033081 DRSC - Results from RNAi screens. • FBgn0033081 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033081 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3183-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033081 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35563 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/geminin1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35563 modMine - Data generated by the modENCODE project. • FBgn0033081
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	anon-WO0257455.1   CG3183 (Boutros et al., 2004, Dimova et al., 2003, Egger et al., 2002, O'Farrell, 2001.4.12, Ni et al., 2011.7.19, Ni et al., 2010.12.1) Dm Geminin (Richardson et al., 2002) Dm geminin (Quinn et al., 2001) Gem (Calvi et al., 2005, Narbonne-Reveau et al., 2008, May et al., 2005, Zielke et al., 2008) gem (Remaud et al., 2008, Herr et al., 2010) geminin (Liu et al., 2005, Machida et al., 2005, Mihaylov et al., 2002, Rendina et al., 2010, Somma et al., 2008, Hall et al., 2008, Ding and Macalpine, 2010, Bateman et al., 2012) Geminin (Zielke et al., 2008, Cunha-Ferreira et al., 2009, Beck et al., 2010) l(2)k14019 (Spradling et al., 1999, Roch et al., 1998, ) v(2)k09107 (Spradling, 1999.12.9, Spradling et al., 1999, )
Name Synonym	geminin (Mihaylov et al., 2002, Somma et al., 2008, Narbonne-Reveau et al., 2008) Geminin (Garcia et al., 2007, Cunha-Ferreira et al., 2009, Narbonne-Reveau et al., 2008, May et al., 2005, Zielke et al., 2008, McCleland et al., 2009, Beck et al., 2010, Herr et al., 2010, Monk et al., 2010)
Secondary FlyBase IDs
	FBgn0021864 FBgn0028557 FBgn0063803
References ( 61 )
Generate a list of	All references relating to this gene ( 61 )
List References by type	Research paper  ( 28 ) Supplementary material  ( 6 ) Review  ( 6 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 3 ) FlyBase analysis  ( 3 ) Conference report  ( 1 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Patent  ( 1 )
Recent research papers ( 5 )
	Bateman et al., 2012, G3 (Bethesda) 2(7): 731--740 A genome-wide screen identifies genes that affect somatic homolog pairing in Drosophila. [FBrf0219075] Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Liu et al., 2012, Mol. Biol. Cell 23(1): 200--212 Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex. [FBrf0217098] Yuan et al., 2012, J. Cell Biol. 198(6): 973--980 Different cyclin types collaborate to reverse the S-phase checkpoint and permit prompt mitosis. [FBrf0219453] Zielke et al., 2011, Nature 480(7375): 123--127 Control of Drosophila endocycles by E2F and CRL4CDT2. [FBrf0216829] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...