Gem, v(2)k09107, l(2)k14019
limits DNA replication by preventing Mcm loading onto chromatin - an inhibitor of the origin binding protein Double parked/Cdt1
Please see the JBrowse view of Dmel\geminin for information on other features
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Gene model reviewed during 5.48
Gene model reviewed during 5.53
The group(s) of polypeptides indicated below share identical sequence to each other.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\geminin using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as embryonic central brain mushroom body
Comment: G2 phase of mitotic cell cycle
geminin protein is expressed in the nuclei of mitotic cells in multiple tissues and developmental stages.
geminin protein is expressed in the nuclei of neuroblasts during S and G2 phases
geminin protein is present in late S phase and G2 cells, but absent from G1-arrested cells. Expression is excluded from the ZNC, a zone of cells arrested in G1 or G2, along the dorsal/ventral compartment boundary in the wing disc.
GBrowse - Visual display of RNA-Seq signals
View Dmel\geminin in GBrowse 2
2-55
2-55
2-56.0
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal antibody
Source for identity of: geminin CG3183
Source for merge of: geminin l(2)k14019 v(2)k09107
Source for merge of: geminin anon-WO0257455.1
Source for identity of geminin CG3183 was sequence comparison ( date:010908 ).
Source for merge of geminin anon-WO0257455.1 was sequence comparison ( date:051113 ).
geminin injection into embryos during interphase 13 does not affect the progression of nuclei through mitosis 13, but does delete the following S phase in cycle 14. Likewise, geminin injection into embryos at the beginning of cycle 14 results in cells collecting in a prolonged mitosis 15 with unreplicated chromatids. (Cellularization, gastrulation mitosis 14 and germband extension are not disturbed in these embryos.)
geminin injection into embryos in cycle 11 or 12 results in a shortened interphase and a prolonged mitosis in the subsequent cycle.
geminin injection into embryos during early mitotic cycles deletes S phase and promotes premature DNA condensation and advances mitotic entry. In the cycle after geminin injection, nuclei enter mitosis with single sister chromatids and fail to bi-orient. Mitotic progression is delayed, but occurs nonetheless.
Embryos injected with geminin during cycle 13 progress normally through mitosis 13, and then nuclei at the injected pole synchronously enter mitosis ~16.5 minutes after the onset of cycle 14, whereas mitosis 14 normally occurs in a spatial program beginning 70 minutes after mitosis 13. Only one extra synchronous division is observed, followed by cellularization, only slightly delayed compared to the uninjected end of the embryo. A subsequent division exhibits a normal mitosis 14 domain pattern.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
geminin has roles in limiting DNA replication, in anaphase and in neural differentiation.
Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology.
