Gene Dmel\Tsp42Eo

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General Information

Symbol

Dmel\Tsp42Eo

Species

D. melanogaster

Name

Tetraspanin 42Eo

Annotation symbol

CG12838

Feature type

protein_coding_gene

FlyBase ID

FBgn0033136

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

42E7-42E7

Sequence location

2R:2,942,222..2,944,081 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Tetraspanin 42Eo is referred to in FlyBase by the symbol Dmel\Tsp42Eo (CG12838, FBgn0033136). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Tetraspanin; Tetraspanin/Peripherin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, at stages throughout the larval period, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1631497_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Tsp42Eo. Gene sequence location is 2R:2942222..2944081.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42E7-42E7

Limits computationally determined from genome sequence between P{lacW}vimar^k16722 and P{lacW}cos^k16101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Tsp42Eo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Tsp42Eo-RA

FBtr0089035

NM_078917

889

219

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Tsp42Eo-PA

FBpp0088106

24.5

219

7.16

NP_523641

AAF59300

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tetraspanin/Peripherin (IPR018499)

Tetraspanin (IPR000301)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		8.75
Larval Midgut		13.3
Larval Hindgut		57.7
Larval Malpighian Tubules		10.7
Larval Fat Body		18.3
Larval Salivary Gland		14.1
Larval Trachea		13.85
Larval Carcass		219.85
Adult Head		11.8
Adult Eye		6.7
Adult Brain		2.8
Adult Thoracic-Abdominal Ganglion		3.9
Adult Crop		68.8
Adult Midgut		13.3
Adult Hindgut		68.7
Adult Malpighian Tubules		4.5
Adult Fat Body		8.7
Adult Salivary Gland		10.8
Adult Heart		47.95
Adult VirginFemale Spermatheca		44.3
Adult InseminatedFemale Spermatheca		50.4
Adult Ovary		3.8
Adult Testis		5.3
Adult Male Accessory Gland		9.2
Adult Carcass		23.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	11.8
NA	Eye	6.7
NA	Brain	2.8
8.75	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	3.9
NA	Crop	68.8
13.3	Midgut	no informative data
57.7	Hindgut	68.7
no informative data	Malpighian Tubules	4.5
18.3	Fat Body	8.7
no informative data	Salivary Gland	no informative data
NA	Heart	47.95
13.85	Trachea	NA
NA	VirginFemale Spermatheca	44.3
NA	InseminatedFemale Spermatheca	50.4
NA	Ovary	3.8
NA	Testis	5.3
NA	Male Accessory Gland	9.2
219.85	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		7
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, at stages throughout the larval period, during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		(50)
embryo 20-22hr		(30)
embryo 22-24hr		(30)
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		(50)
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		1
embryo 12-14hr		1
embryo 14-16hr		4
embryo 16-18hr		15
embryo 18-20hr		50
embryo 20-22hr		30
embryo 22-24hr		30
larva L1		21
larva L2		17
larva L3 12hr old		21
larva L3 puffstage 1-2		20
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		20
white prepupae new		10
white prepupae 12hr		21
white prepupae 24hr		7
pupae 2d postWPP		7
pupae 3d postWPP		12
pupae 4d postWPP		17
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		4
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		1
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		1
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		6
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		0
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_18

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033136

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12838-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Tsp42Eo^GD3754

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Tsp42Eo	Class	Mutagen	Stocks	Known lesion
Tsp42Eo^G19478		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Tsp42Eo	Mutagen	Stocks	Known lesion
Tsp42Eo^GD3754	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Tsp42Eo^KK111463	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Tsp42Eo^NIG.12838R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)Exel6283

(Ryder, 2004.4.26)

Df(2R)Δ42E

(Lin et al., 2005)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD3754}

P{KK111463}

P{NIG.12838R}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Tsp42Eo^G19478

Gene Ontology: Function, Process & Cellular Component ( 1 unique term )

There is no information supporting a biological process or molecular function term for this gene.

(Based on a review of available data on: 2011-06-30)

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

integral to membrane

inferred from electronic annotation with InterPro:IPR000301, InterPro:IPR018499

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Fradkin et al., 2002)

inferred from sequence or structural similarity

(Todres et al., 2000)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Tsp42Eo allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0033136

Orthologs

OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6S4Q2J

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

EOG6SJ3X1

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6S4Q2J)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11844

Drosophila persimilis

Dper\GL11149

Drosophila willistoni

Dwil\GK21786

Drosophila virilis

Dvir\GJ15140

Drosophila mojavensis

Dmoj\GI19480

Drosophila grimshawi

Dgri\GH10375

Drosophila grimshawi

Dgri\GH21510

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (EOG6SJ3X1)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW001003

Ixodes scapularis

Deer tick

Isca\ISCW009188

Ixodes scapularis

Deer tick

Isca\ISCW009186

Ixodes scapularis

Deer tick

Isca\ISCW009187

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11844

Drosophila persimilis

Dper\GL11149

Drosophila willistoni

Dwil\GK21786

Drosophila virilis

Dvir\GJ15140

Drosophila mojavensis

Dmoj\GI19480

Drosophila grimshawi

Dgri\GH21510

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

28424

y¹ w^*; P{EP}Tsp42Eo^G19478

VDRC

v103222

P{KK111463}VIE-260B

v8722

w¹¹¹⁸; P{GD3754}v8722/CyO

Genomic Clones ( 3 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 6 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0033136

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

35625

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Tsp42Eo CG12838

(Todres et al., 2000)

Source for database merge of

Source for merge of: Tsp42Eo BcDNA:RH51444

(FlyBase, 1996-)

Additional comments

Source for merge of Tsp42Eo BcDNA:RH51444 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_078917

RefSeq (Proteins)

•

NP_523641

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

35625

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tetraspanin/Peripherin (IPR018499)

Tetraspanin (IPR000301)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0033136

DRSC - Results from RNAi screens.

•

FBgn0033136

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033136

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12838-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0033136

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

35625

modMine - Data generated by the modENCODE project.

•

FBgn0033136

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

BcDNA:RH51444

CG12838

(Fradkin et al., 2002, Todres et al., 2000, Keleman et al., 2009.8.5)

Dm.Tsp42Eo

(Todres et al., 2000)

Tsp42Eo

(Tokusumi et al., 2012, Japanese National Institute of Genetics, 2012.5.21)

Name Synonym

tetraspanin 42E

(Silverman et al., 2003)

Tetraspanin 42Eo

(Todres et al., 2000)

Secondary FlyBase IDs

FBgn0062933

References ( 20 )

Research paper

Tokusumi et al., 2012, PLoS ONE 7(7): e41604: Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204]
Lin et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(36): 12831--12836: A Drosophila DEG/ENaC channel subunit is required for male response to female pheromones. [FBrf0188263]
Silverman et al., 2003, J. Biol. Chem. 278(49): 48928--48934: Immune activation of NF-kappaB and JNK requires Drosophila TAK1. [FBrf0167753]
Fradkin et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99(21): 13663--13668: Genomewide analysis of the Drosophila tetraspanins reveals a subset with similar function in the formation of the embryonic synapse. [FBrf0152316]
Todres et al., 2000, Insect Mol. Biol. 9(6): 581--590: The tetraspanin superfamily in insects. [FBrf0132356]

Supplementary material

The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]

Personal communication to FlyBase

Bellen, 2009.8.20, BDGP_2009_0825.GenExel.xml: BDGP_2009_0825.GenExel.xml [FBrf0208536]
Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Robertson, 2000.4.14, FlyBase error report for CG12838 on Fri Apr 14 13:41:28 2000.: FlyBase error report for CG12838 on Fri Apr 14 13:41:28 2000. [FBrf0128964]

FlyBase analysis

FlyBase Curators, 2010-, Gene Ontology (GO) annotation review.: Gene Ontology (GO) annotation review. [FBrf0216433]
FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase.: Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Stock list

Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.: RNAi stocks available from NIG-FLY. [FBrf0218457]

Curated genome annotation

FamiliarityBreedsContempt, 1999.11, [Automatic annotations performed at the Celera Jamboreee.]: [Automatic annotations performed at the Celera Jamboreee.] [FBrf0126705]