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Gene Dmel\Eaf

General Information
Symbol	Dmel\Eaf	Species	D. melanogaster
Name	ELL-associated factor	Annotation symbol	CG11166
Feature type	protein_coding_gene	FlyBase ID	FBgn0033166
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dEaf
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	43B2-43B2	Sequence location	2R:3,285,731..3,295,326 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ELL-associated factor is referred to in FlyBase by the symbol Dmel\Eaf (CG11166, FBgn0033166). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: regulation of DNA-dependent transcription in response to stress. 5 alleles are reported. The phenotype of these alleles is annotated with: trichogen cell. It has 8 annotated transcripts and 8 annotated polypeptides. Protein features are: Transcription elognation factor Eaf, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639947_s_at completely aligns to an exonic region common to each of the 8 FlyBase-annotated transcript isoforms of Eaf. Gene sequence location is 2R:3285731..3295326.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Sequence features HFA06052 HFA05050 BKN46090 BKN27861
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 43B2-43B2   Limits computationally determined from genome sequence between P{lacW}cosk16101 and P{EP}CG1600EP398
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Eaf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	EST data suggest additional 3' exon(s)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eaf-RA FBtr0089008  NM_165518   3101   504 Eaf-RB FBtr0089009  NM_165521   2043   450 Eaf-RC FBtr0089006  NM_165519   3174   504 Eaf-RD FBtr0089007  NM_136429   2459   504 Eaf-RE FBtr0089010  NM_165520   2563   504 Eaf-RF FBtr0089011  NM_206049   3097   504 Eaf-RG FBtr0089012  NM_206048   3105   504 Eaf-RH FBtr0300697  NM_001169599   2495   504
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Eaf-PA   FBpp0088080   54.9   504   7.36     NP_724547   AAF59263 Eaf-PB   FBpp0088081   48.8   450   7.00     NP_724550   AAF59266 Eaf-PC   FBpp0088078   54.9   504   7.36     NP_724548   AAM68888 Eaf-PD   FBpp0088079   54.9   504   7.36     NP_610273   AAM68889 Eaf-PE   FBpp0088082   54.9   504   7.36     NP_724549   AAM68890 Eaf-PF   FBpp0088083   54.9   504   7.36     NP_995771   AAS64902 Eaf-PG   FBpp0088084   54.9   504   7.36     NP_995770   AAS64903 Eaf-PH   FBpp0289921   54.9   504   7.36     NP_001163070   ACZ94350
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription elognation factor Eaf, N-terminal (IPR019194)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246302   AA390881   AA538709   AA817344   AA820203   AA942268   AE013599 AAF59263   AE013599 AAF59266   AE013599 AAM68888   AE013599 AAM68889   AE013599 AAM68890   AE013599 AAS64902   AE013599 AAS64903   AE013599 ACZ94350   AI064429   AI259238   AI260536   AI294934   AI455467   AI456217   AI456936   AI515186   AI518808   AI519733   AI520447   AI532479   AI546160   AQ073618   AW943266   BF487056   BF494263   BG638167   BI166214   BI167872   BI170056   BI170807   BI232164   BI237414   BI237419   BI241297   BI373500   BI564964   BI605048   BI625378   BI639526   BT001503 AAN71258   BT001556 AAN71311   BT003263 AAO25020   CO156941   CO177512   CO178681   CO263858   CO267253   CO267574   CO269610   CO277770   CO279354   CO279996   CO282043   CO287923   CO288572   CO289372   CO300423   CO303693   CO304161   CO312080   CO312997   CO313051   CO315950   CO318563   CO320430   CO324443   CO338825   EC057145   EC058619   EC059867   EC064233   EC065029   EC069623   EC076550   EC080317   EC088004   EC089779   EC198287   EC205044   EC207533   EC209206   EC210553   EC210890   EC216029   EC219939   EC221105   EC222857   EC223788   EC225349   EC227377   EC228720   EC241691   EC243679   EC244594   EC245003   EC248443   EC262198   EC264039   EL874727   EL874849   EV592695   EV592963   EV593013   GH945430   GH946236   GH959618   GH974106   GH978901   GH984968
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JRJ1 E1JGZ1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0033166    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0033166 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0033166 expression level Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4222) embryo 02-04hr  (4568) embryo 04-06hr  (3008) embryo 06-08hr  (3075) embryo 08-10hr  (2766) embryo 10-12hr  (1999) embryo 12-14hr  (2563) embryo 14-16hr  (3543) embryo 16-18hr  (3000) embryo 18-20hr  (2546) embryo 20-22hr  (2148) embryo 22-24hr  (1820) larva L1  (1506) larva L2  (1661) larva L3 12hr old  (1569) larva L3 puffstage 1-2  (951) larva L3 puffstage 3-6  (1759) larva L3 puffstage 7-9  (1585) white prepupae new  (1570) white prepupae 12hr  (1179) white prepupae 24hr  (1509) pupae 2d postWPP  (2114) pupae 3d postWPP  (1858) pupae 4d postWPP  (1332) adult male 01day  (1369) adult male 05day  (1488) adult male 30day  (1470) adult female 01day  (2058) adult female 05day  (2519) adult female 30day  (2716) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4222) embryo 02-04hr  (4568) embryo 04-06hr  (3008) embryo 06-08hr  (3075) embryo 08-10hr  (2766) embryo 10-12hr  (1999) embryo 12-14hr  (2563) embryo 14-16hr  (3543) embryo 16-18hr  (3000) embryo 18-20hr  (2546) embryo 20-22hr  (2148) embryo 22-24hr  (1820) larva L1  1506 larva L2  (1661) larva L3 12hr old  (1569) larva L3 puffstage 1-2    951 larva L3 puffstage 3-6  (1759) larva L3 puffstage 7-9  (1585) white prepupae new  (1570) white prepupae 12hr    1179 white prepupae 24hr  1509 pupae 2d postWPP  (2114) pupae 3d postWPP  (1858) pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day  1488 adult male 30day  1470 adult female 01day  (2058) adult female 05day  (2519) adult female 30day  (2716) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0033166 expression level Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4222) embryo 02-04hr  (4568) embryo 04-06hr  (3008) embryo 06-08hr  (3075) embryo 08-10hr  (2766) embryo 10-12hr  (1999) embryo 12-14hr  (2563) embryo 14-16hr  (3543) embryo 16-18hr  (3000) embryo 18-20hr  (2546) embryo 20-22hr  (2148) embryo 22-24hr  (1820) larva L1  (1506) larva L2  (1661) larva L3 12hr old  (1569) larva L3 puffstage 1-2  (951) larva L3 puffstage 3-6  (1759) larva L3 puffstage 7-9  (1585) white prepupae new  (1570) white prepupae 12hr  (1179) white prepupae 24hr  (1509) pupae 2d postWPP  (2114) pupae 3d postWPP  (1858) pupae 4d postWPP  (1332) adult male 01day  (1369) adult male 05day  (1488) adult male 30day  (1470) adult female 01day  (2058) adult female 05day  (2519) adult female 30day  (2716) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4222) embryo 02-04hr  (4568) embryo 04-06hr  (3008) embryo 06-08hr  (3075) embryo 08-10hr  (2766) embryo 10-12hr  1999 embryo 12-14hr  (2563) embryo 14-16hr  (3543) embryo 16-18hr  (3000) embryo 18-20hr  (2546) embryo 20-22hr  2148 embryo 22-24hr  1820 larva L1  1506 larva L2  1661 larva L3 12hr old  1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6  1759 larva L3 puffstage 7-9  1585 white prepupae new  1570 white prepupae 12hr    1179 white prepupae 24hr  1509 pupae 2d postWPP  2114 pupae 3d postWPP  1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day  1488 adult male 30day  1470 adult female 01day  2058 adult female 05day  (2519) adult female 30day  (2716) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4222 embryo 02-04hr    4568 embryo 04-06hr    3008 embryo 06-08hr    3075 embryo 08-10hr    2766 embryo 10-12hr    1999 embryo 12-14hr    2563 embryo 14-16hr    3543 embryo 16-18hr    3000 embryo 18-20hr    2546 embryo 20-22hr    2148 embryo 22-24hr    1820 larva L1    1506 larva L2    1661 larva L3 12hr old    1569 larva L3 puffstage 1-2    951 larva L3 puffstage 3-6    1759 larva L3 puffstage 7-9    1585 white prepupae new    1570 white prepupae 12hr    1179 white prepupae 24hr    1509 pupae 2d postWPP    2114 pupae 3d postWPP    1858 pupae 4d postWPP    1332 adult male 01day    1369 adult male 05day    1488 adult male 30day    1470 adult female 01day    2058 adult female 05day    2519 adult female 30day    2716 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0033166    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0033166 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0033166 expression level Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0033166 expression level Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    71.025 Larval Midgut    101.8 Larval Hindgut    119.3 Larval Malpighian Tubules    127.7 Larval Fat Body    198.3 Larval Salivary Gland    214.2 Larval Trachea    111.7 Larval Carcass    164.025 Adult Head    88.7 Adult Eye    85.875 Adult Brain    81.1 Adult Thoracic-Abdominal Ganglion    63.6 Adult Crop    143.9 Adult Midgut    104.9 Adult Hindgut    111.2 Adult Malpighian Tubules    131.9 Adult Fat Body    136.5 Adult Salivary Gland    211.4 Adult Heart    114.875 Adult VirginFemale Spermatheca    124.9 Adult InseminatedFemale Spermatheca    149.5 Adult Ovary    364.8 Adult Testis    125.2 Adult Male Accessory Gland    228.2 Adult Carcass    115.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    88.7   NA  Eye    85.875   NA  Brain    81.1   71.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    63.6   NA  Crop    143.9   101.8  Midgut    104.9   119.3  Hindgut    111.2   127.7  Malpighian Tubules    131.9   198.3  Fat Body    136.5   214.2  Salivary Gland    211.4   NA  Heart    114.875   111.7  Trachea    NA   NA  VirginFemale Spermatheca    124.9   NA  InseminatedFemale Spermatheca    149.5   NA  Ovary    364.8   NA  Testis    125.2   NA  Male Accessory Gland    228.2   164.025  Carcass    115.7 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033166 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11166-RC
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act5C.PU EafdsRNA.Sym.Scer\UAS viable, with Scer\GAL4pnr-MD237 EafGD7220 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 EafGD7220 Phenotype manifest in Allele trichogen cell, with Scer\GAL4pnr-MD237 EafGD7220
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Eaf Class Mutagen Stocks Known lesion EafEP2475 P-element activity 1 -- EafG8126 P-element activity 1 -- EafCPTI000560 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eaf Class Mutagen Stocks Known lesion EafGD7220 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes EafdsRNA.Sym.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{Sym-UAS-Eaf} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EafEP2475 NA P{EP}EafG8126 NA miscellaneous insertions PBac{602.P.SVS-1}EafCPTI000560 NA
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
regulation of DNA-dependent transcription in response to stress inferred from mutant phenotype (Smith et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Eaf allele Gene References CG11166EP2475 pnr (Pena-Rangel et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61540 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033166 DroID - A comprehensive database of gene and protein interactions. • FBgn0033166 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35660
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12230 Dere\GG10755 Dgri\GH20739 Dmoj\GI20822 Dper\GL10937 Dpse\GA24539 Dsec\GM20800 Dsim\GD10262 Dvir\GJ20555 Dwil\GK22015 Dyak\GE24070
Curated drosophilid orthologs	Dpse\GA10811
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL003973-PB Aedes aegypti (mosquito) XP_394906.2 Apis mellifera (honey bee) XP_001948068.1 Acyrthosiphon pisum (pea aphid) At1g71080.1 Arabidopsis thaliana (wall cress) 27858 Batrachochytrium dendrobatidis (chytrid fungus) 92034 Branchiostoma floridae (lancelet) XP_001896174.1 Brugia malayi (roundworm nematode) BGIBMGA005231-PA Bombyx mori (silk worm) ENSBTAP00000017380 Bos taurus (bovine) CN26864 Caenorhabditis brenneri (small nematode) CN28508 Caenorhabditis brenneri (small nematode) CBP12067 Caenorhabditis briggsae (nematode) CE40450 Caenorhabditis elegans (worm) ENSCINP00000018573 Ciona intestinalis (sea squirt) CPIJ020269 Culex pipens (mosquito) ENSCPOP00000005791 Cavia porcellus (guinea pig) RP03085 Caenorhabditis remanei (nematode) 159180 Capitella spl (polychaete worm) 113677 Daphnia pulex (daphnia) ENSDARP00000095027 Danio rerio (zebra fish) ENSECAP00000012788 Equus caballus (horse) ENSGACP00000002208 Gymnopilus aculeatus (mushroom) ENSP00000273668 Homo sapiens (human) ENSMODP00000018167 Monodelphis domestica (gray opossum) ENSMODP00000018169 Monodelphis domestica (gray opossum) ENSMMUP00000001406 Macaca mulatta (rhesus monkey) XP_001601157.1 Nasonia vitripennis (wasp) ENSOANP00000024407 Ornithorynchus anatinus (platypus) ENSORLP00000012587 Oryzias latipes (japanese killifish) LOC_Os01g32300.1 Oryza sativa (asian rice) PP23093 Pristionchus pacificus (nematode) ENSPPYP00000015091 Pongo pygmaeus (orangutan) 555923 Populus trichocarpa (black cottonwood) ENSPTRP00000026347 Pan troglodytes (chimpanzee) Smp_180480.2 Schistosoma mansoni (trematode parasite) SPCC1223.10c Schizosaccharomyces pombe (fission yeast) XP_787179.1 Strongylocentrotus purpuratus (purple sea urchin) 55027 Trichoplax adhaerens (metazoa) XP_967672.1 Tribolium castaneum (red flour beetle) ENSTRUP00000008593 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000047639 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14367 NV13113 LH17474 ISCW010442 FBgn0241209 FBgn0224000 FBgn0207695 FBgn0182036 FBgn0175681 JGI_V11_113677 FBgn0245930 FBgn0148547 FBgn0143557 FBgn0128202 FBgn0103060 FBgn0089269 CPIJ020269 CPIJ006190 CPIJ006191 BGIBMGA005231 ACYPI006338 GB18187 AGAP012061 AAEL003973 PHUM497540 GLEAN_15812
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	17250 w1118; P{EP}EafEP2475/CyO 28493 y1 w*; P{EP}EafG8126 CG11112G8126
VDRC	v31436 w1118; P{GD7220}v31436 v31435 w1118; P{GD7220}v31435/CyO
Genomic Clones ( 2 )
	BACR04G20 BACR26B18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 101 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22594 LD35207 RE12395
Other clones	DPiM_LD22594
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02708 AT21730 GH04855 LD05164 LD09086 LD23693 LD24408 LD26376 LD36088 LD37108 LD39448 LD40618 LD46257 LD46903 LP02610 LP04441 LP08425 RE06263 RE08359 RE11402 RE28182 RE33640 RE33646 RE38331 RE60808 RH62568 RH65766 RH70563 SD04015 SD22125
Other clones	5849 7955 15730 20057 21373 37212 38049 44220 53886 66066 80073 103090 104571 105806 105954 173248 174605 179025 194231 296215 533492 CK01.19D.D10 CK01.26B.B12 CK01.66A.E5 CK01.72B.A2 CK01.84B.C11 CK01.84D.C11 CK01.117B.G2 CK01.120C.E1 CK01.132B.F8 Dmel_neb_009927_0682_3444 Dmel_neb_013899_0626_2917 Dmel_neb_014013_0579_0686 EK053719 EK057333 EK067310 EK068910 EK070901 EK095156 EK126253 EK134135 EK136705 EK149241 EK150850 EK152206 EK159411 EK178152 EK193662 EK227843 EK230911 EK231031 EK242531 EK244019 EK263207 EK272817 EK284858 EN09621 EN17706 IP14827 IP14927 LD18301 RP004221555 RP004231060 RP004255948 RP004370217 RP004428306 RP004555346
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033166 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35660
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Eaf CG11166 (Smith et al., 2008)
Source for database merge of	Source for merge of: CG11166 CG1706 (FlyBase, 1996-)
Additional comments	Source for merge of CG11166 CG1706 was sequence comparison (date:001104). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136429 NM_165518 NM_165519 NM_165520 NM_165521 NM_206048 NM_206049 NM_001169599 RefSeq (Proteins) • NP_610273 NP_724547 NP_724548 NP_724549 NP_724550 NP_995770 NP_995771 NP_001163070 DDBJ / EMBL / GenBank • AA817344 AAF59263 AAF59266 AAM68888 AAM68889 AAM68890 AAN71258 AAN71311 AAO25020 AAS64902 AAS64903 ACZ94350 AI518808 AQ073618 AW943266 BI170807 BT001503 BT001556 BT003263 Entrez Gene - A searchable database of RefSeq genes. • 35660 UniProtKB/TrEMBL • E1JGZ1 Q7JRJ1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription elognation factor Eaf, N-terminal (IPR019194)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61540 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033166 DroID - A comprehensive database of gene and protein interactions. • FBgn0033166 DRSC - Results from RNAi screens. • FBgn0033166 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033166 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11166-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033166 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35660 InParanoid A subset of ortholog calls from InParanoid. • AAEL003973-PB Aedes aegypti (mosquito) XP_394906.2 Apis mellifera (honey bee) XP_001948068.1 Acyrthosiphon pisum (pea aphid) At1g71080.1 Arabidopsis thaliana (wall cress) 27858 Batrachochytrium dendrobatidis (chytrid fungus) 92034 Branchiostoma floridae (lancelet) XP_001896174.1 Brugia malayi (roundworm nematode) BGIBMGA005231-PA Bombyx mori (silk worm) ENSBTAP00000017380 Bos taurus (bovine) CN26864 Caenorhabditis brenneri (small nematode) CN28508 Caenorhabditis brenneri (small nematode) CBP12067 Caenorhabditis briggsae (nematode) CE40450 Caenorhabditis elegans (worm) ENSCINP00000018573 Ciona intestinalis (sea squirt) CPIJ020269 Culex pipens (mosquito) ENSCPOP00000005791 Cavia porcellus (guinea pig) RP03085 Caenorhabditis remanei (nematode) 159180 Capitella spl (polychaete worm) 113677 Daphnia pulex (daphnia) ENSDARP00000095027 Danio rerio (zebra fish) ENSECAP00000012788 Equus caballus (horse) ENSGACP00000002208 Gymnopilus aculeatus (mushroom) ENSP00000273668 Homo sapiens (human) ENSMODP00000018167 Monodelphis domestica (gray opossum) ENSMODP00000018169 Monodelphis domestica (gray opossum) ENSMMUP00000001406 Macaca mulatta (rhesus monkey) XP_001601157.1 Nasonia vitripennis (wasp) ENSOANP00000024407 Ornithorynchus anatinus (platypus) ENSORLP00000012587 Oryzias latipes (japanese killifish) LOC_Os01g32300.1 Oryza sativa (asian rice) PP23093 Pristionchus pacificus (nematode) ENSPPYP00000015091 Pongo pygmaeus (orangutan) 555923 Populus trichocarpa (black cottonwood) ENSPTRP00000026347 Pan troglodytes (chimpanzee) Smp_180480.2 Schistosoma mansoni (trematode parasite) SPCC1223.10c Schizosaccharomyces pombe (fission yeast) XP_787179.1 Strongylocentrotus purpuratus (purple sea urchin) 55027 Trichoplax adhaerens (metazoa) XP_967672.1 Tribolium castaneum (red flour beetle) ENSTRUP00000008593 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000047639 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35660 modMine - Data generated by the modENCODE project. • FBgn0033166 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14367 NV13113 LH17474 ISCW010442 FBgn0241209 FBgn0224000 FBgn0207695 FBgn0182036 FBgn0175681 JGI_V11_113677 FBgn0245930 FBgn0148547 FBgn0143557 FBgn0128202 FBgn0103060 FBgn0089269 CPIJ020269 CPIJ006190 CPIJ006191 BGIBMGA005231 ACYPI006338 GB18187 AGAP012061 AAEL003973 PHUM497540 GLEAN_15812
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG1706 (Whitfield, 2000.10.31, ) CG11166   dEaf (Smith et al., 2008, Campos et al., 2010) Eaf (Smith et al., 2008, Campos et al., 2010) EP(2)2475 (Pena-Rangel et al., 2002) LD35207 (Pena-Rangel et al., 2002)
Name Synonym	CG11166   dELL-associated factor (Smith et al., 2008) ELL-associated factor
Secondary FlyBase IDs
	FBgn0033163
References ( 15 )
Research paper	Campos et al., 2010, Genetics 184(1): 129--140 Genetic screen in Drosophila melanogaster uncovers a novel set of genes required for embryonic epithelial repair. [FBrf0209682] Smith et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(25): 8575--8579 Regulation of the transcriptional activity of poised RNA polymerase II by the elongation factor ELL. [FBrf0205490] Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050 A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	FlyProt project., 2011.8.8, CPTI protein trap lines available from the Kyoto Stock Center. CPTI protein trap lines available from the Kyoto Stock Center. [FBrf0214588] Knowles-Barley, 2011.8.24, BrainTrap expression curation. BrainTrap expression curation. [FBrf0215202] Bellen, 2009.8.20, BDGP_2009_0825.GenExel.xml BDGP_2009_0825.GenExel.xml [FBrf0208536] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Whitfield, 2000.10.31, FlyBase error report on Tue Oct 31 16:26:11 2000. FlyBase error report on Tue Oct 31 16:26:11 2000. [FBrf0132113]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]