FlyBase Gene Report: Dmel\Nup44A

NUCLEAR PORE COMPLEX -: The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).
GATOR2 COMPLEX -: GATOR2 is a subcomplex of the GATOR or SEA complex. It suppresses the Rag GTPase activating protein activity of the GATOR1 subcomplex, promoting TORC1 activity. (Adapted from FBrf0232362 and PMID:25934700).

Protein Function (UniProtKB)

Probable component of the nuclear pore complex (NPC) (By similarity). Involved in maintaining the localization of another nucleoporin Mtor to the nuclear envelope of early meiotic female germline cells (PubMed:21521741). It is not involved in recruiting the nucleoporins Mtor, Nup107, Nup153 and FG-containing nucleoporins to the NPC (PubMed:21521741). An essential component of the GATOR subcomplex GATOR2 which functions as an activator of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:27166823, PubMed:23723238). The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:27166823, PubMed:23723238, PubMed:25512509). GATOR2 activates the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, controlling the switch to cell proliferation growth under nutrient replete conditions and growth during female oocyte development (PubMed:21521741, PubMed:25512509, PubMed:23723238, PubMed:27166823). This component is required for activating TORC1 specifically in germline cells to promote cell growth and maintain the oocyte fate, probably influences the organization and/or function of microtubules within ovarian cysts, and promotes accumulation of another GATOR2 complex member mio in germline and somatic tissues (PubMed:27166823, PubMed:23723238, PubMed:25512509, PubMed:21521741). GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:25512509). After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509). At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:21521741, PubMed:25512509). In addition to its role in the regulation of the TORC1 complex, functions independently of TORC1 to prevent the inappropriate accumulation of autolysosomes in germline tissues (PubMed:27166823).

(UniProt, Q7K2X8)

Summary (Interactive Fly)

component of a nucleoporin subcomplex - a binding parter of Missing oocyte; regulates nuclear architecture and meiotic progression in early ovarian cysts

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

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Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nup44A-RA

FBtr0088862

NM_136499

1208

354

Nup44A-RB

FBtr0088860

NM_165582

1283

354

Nup44A-RC

FBtr0088861

NM_165583

1331

354

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Nup44A-PA

FBpp0087938

39.5

354

6.70

NP_610343

AAF59154

Nup44A-PB

FBpp0087936

39.5

354

6.70

NP_724637

AAM68880

Nup44A-PC

FBpp0087937

39.5

354

6.70

NP_724638

AAM68881

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

354 aa isoforms: Nup44A-PA, Nup44A-PB, Nup44A-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Probable component of the nuclear pore complex (NPC) (By similarity). Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823). Within the GATOR complex, probable component of the GATOR2 subcomplex which is likely composed of mio, Nup44A/Seh1, Wdr24, Wdr59 and Sec13 (PubMed:27166823). Interacts with mio (PubMed:21521741). Interacts with Wdr24 (PubMed:27166823).

(UniProt, Q7K2X8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup44A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (20 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

structural molecule activity

inferred from electronic annotation with InterPro:IPR037363

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

cellular response to amino acid starvation