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Gene Dmel\Nup50

General Information
Symbol	Dmel\Nup50	Species	D. melanogaster
Name	Nucleoporin 50kD	Annotation symbol	CG2158
Feature type	protein_coding_gene	FlyBase ID	FBgn0033264
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	44B2-44B3	Sequence location	2R:4,010,541..4,012,563 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Nucleoporin 50kD is referred to in FlyBase by the symbol Dmel\Nup50 (CG2158, FBgn0033264). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: SMAD protein import into nucleus; neurogenesis; phagocytosis, engulfment. 5 alleles are reported. The phenotype of these alleles is annotated with: tormogen cell. It has one annotated transcript and one annotated polypeptide. Protein features are: Nuclear pore complex, NUP2/50/61; Pleckstrin homology-like domain; Ran binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1630141_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Nup50. Gene sequence location is 2R:4010541..4012563.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44B2-44B3   Limits computationally determined from genome sequence between P{lacW}Vps28k16503 and P{EP}kermitEP493
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nup50 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nup50-RA FBtr0088783  NM_136511   1907   564
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nup50-PA   FBpp0087861   59.4   564   8.73     NP_610355   AAF59131
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ran binding domain (IPR000156) Pleckstrin homology-like domain (IPR011993) Nuclear pore complex, NUP2/50/61 (IPR015007)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264024   AA392599   AA440237   AA440480   AA440500   AA440962   AA735364   AA820291   AA820519   AA820791   AA821022   AA941643   AA941675   AA942265   AA942401   AE013599 AAF59131   AI108399   AI238653   AI388924   AI512991   AI514799   AW942867   AY070546 AAL48017   BF486061   BF499336   BF503100   BG638257   BG638479   BI162812   BI213453   BI213831   BI213978   BI235948   BI239096   BI240202   BI351943   BI357094   BI357133   BI357160   BI357165   BI357180   BI357390   BI361419   BI362005   BI368551   BI371074   BI373249   BI373393   BI373714   BI483319   BI578524   BQ103300   CO154632   CO284361   CO300785   CO315491   CO319797   CO337108   CZ479960   EC068709   EC086132   EC215186   EC237525   EC239091   EC265154   EL873815   EL873816   EL873914   EV576986   EV579390
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K0D8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) longitudinal visceral muscle primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) visceral muscle primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval fat body   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary  502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary  502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    374.35 Larval Midgut    122 Larval Hindgut    183.2 Larval Malpighian Tubules    121.2 Larval Fat Body    85.8 Larval Salivary Gland    363.8 Larval Trachea    245.225 Larval Carcass    230.45 Adult Head    69 Adult Eye    55.6 Adult Brain    87.2 Adult Thoracic-Abdominal Ganglion    87.2 Adult Crop    83.7 Adult Midgut    99.8 Adult Hindgut    84.8 Adult Malpighian Tubules    121.8 Adult Fat Body    128.6 Adult Salivary Gland    108.7 Adult Heart    90.325 Adult VirginFemale Spermatheca    132 Adult InseminatedFemale Spermatheca    154.6 Adult Ovary    502.5 Adult Testis    122.9 Adult Male Accessory Gland    225.4 Adult Carcass    80.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    69   NA  Eye    55.6   NA  Brain    87.2   374.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    87.2   NA  Crop    83.7   122  Midgut    99.8   183.2  Hindgut    84.8   121.2  Malpighian Tubules    121.8   85.8  Fat Body    128.6   363.8  Salivary Gland    108.7   NA  Heart    90.325   245.225  Trachea    NA   NA  VirginFemale Spermatheca    132   NA  InseminatedFemale Spermatheca    154.6   NA  Ovary    502.5   NA  Testis    122.9   NA  Male Accessory Gland    225.4   230.45  Carcass    80.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    116 ovary, mated 4-day female    101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (62) central nervous system, larvae L3  (45) central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering  (35) carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (101) testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  (116) ovary, mated 4-day female  101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    116 ovary, mated 4-day female    101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    116 ovary, mated 4-day female    101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (62) central nervous system, larvae L3  (45) central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering  (35) carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (101) testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  116 ovary, mated 4-day female  101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    62 central nervous system, larvae L3    45 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    11 digestive system, 4-day adult    18 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    35 carcass, 1-day adult    8 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    116 ovary, mated 4-day female    101 testis, mated 4-day male    19 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    111 embryo 02-04hr    129 embryo 04-06hr    118 embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (111) embryo 02-04hr  (129) embryo 04-06hr  (118) embryo 06-08hr  (82) embryo 08-10hr  (64) embryo 10-12hr  (45) embryo 12-14hr  (33) embryo 14-16hr  (33) embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1  (33) larva L2  (31) larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9  26 white prepupae new  26 white prepupae 12hr  26 white prepupae 24hr  (29) pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day  (38) adult female 05day  (69) adult female 30day  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (111) embryo 02-04hr  (129) embryo 04-06hr  (118) embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    111 embryo 02-04hr    129 embryo 04-06hr    118 embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    111 embryo 02-04hr    129 embryo 04-06hr    118 embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (111) embryo 02-04hr  (129) embryo 04-06hr  (118) embryo 06-08hr  (82) embryo 08-10hr  (64) embryo 10-12hr  (45) embryo 12-14hr  (33) embryo 14-16hr  (33) embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1  (33) larva L2  31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9  26 white prepupae new  26 white prepupae 12hr  26 white prepupae 24hr  29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day  (38) adult female 05day  (69) adult female 30day  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  111 embryo 02-04hr  129 embryo 04-06hr  118 embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    111 embryo 02-04hr    129 embryo 04-06hr    118 embryo 06-08hr    82 embryo 08-10hr    64 embryo 10-12hr    45 embryo 12-14hr    33 embryo 14-16hr    33 embryo 16-18hr    24 embryo 18-20hr    12 embryo 20-22hr    21 embryo 22-24hr    22 larva L1    33 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    26 white prepupae new    26 white prepupae 12hr    26 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    38 adult female 05day    69 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (105) Schneider line 2 Sg4  (63) embryonic 1182-4H  (48) embryonic GM2  (43) embryonic Kc167  (36) embryonic S1  (54) embryonic S3  (58) leg disc CME L1  (82) wing disc CME-W2  (76) wing disc ML-DmD8  (48) wing disc ML-DmD9  (56) wing disc ML-DmD16-c3  (49) wing disc ML-DmD21  (60) wing disc ML-DmD32  (59) haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (50) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (65) CNS ML-DmBG1-c1  (69) CNS ML-DmBG2-c2  (65) tumorous blood cells mbn2  (30) ovary fGS/OSS  (51) ovary OSC  (48) ovary OSS  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (105) Schneider line 2 Sg4  (63) embryonic 1182-4H  (48) embryonic GM2  (43) embryonic Kc167  (36) embryonic S1  (54) embryonic S3  (58) leg disc CME L1  (82) wing disc CME-W2  (76) wing disc ML-DmD8  (48) wing disc ML-DmD9  (56) wing disc ML-DmD16-c3  (49) wing disc ML-DmD21  (60) wing disc ML-DmD32  (59) haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (50) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (65) CNS ML-DmBG1-c1  (69) CNS ML-DmBG2-c2  (65) tumorous blood cells mbn2  30 ovary fGS/OSS  (51) ovary OSC  (48) ovary OSS  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    105 Schneider line 2 Sg4    63 embryonic 1182-4H    48 embryonic GM2    43 embryonic Kc167    36 embryonic S1    54 embryonic S3    58 leg disc CME L1    82 wing disc CME-W2    76 wing disc ML-DmD8    48 wing disc ML-DmD9    56 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    60 wing disc ML-DmD32    59 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    65 CNS ML-DmBG1-c1    69 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    30 ovary fGS/OSS    51 ovary OSC    48 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult  (36) Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (59) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (65) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (66) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (57) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult  (36) Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (59) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (65) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (66) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (57) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    13 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    59 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    65 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    66 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD27030 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033264 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2158-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Nup50 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Nup50GD9739 viable, with Scer\GAL4pnr-MD237 Nup50GD9739 Other Phenotypes Allele neuroanatomy defective | larval stage Nup50GD9739 visible, with Scer\GAL4pnr-MD237 Nup50GD9739 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage Nup50GD9739 tormogen cell, with Scer\GAL4pnr-MD237 Nup50GD9739 trichogen cell | supernumerary, with Scer\GAL4pnr-MD237 Nup50GD9739
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Nup50 Class Mutagen Stocks Known lesion Nup50e02135 piggyBac transposase 1 -- Nup50KG09557 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nup50 Class Mutagen Stocks Known lesion Nup50GD9739 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nup50HMS01054 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nup50KK108150 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6056 (Ryder, 2004.4.26) Df(2R)Exel7094 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{TRiP.HMS01054} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}Nup50KG09557 NA PBac{RB}Nup50e02135 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Z600 (Gawlinski et al., 2007)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) SMAD protein import into nucleus inferred from mutant phenotype (Chen and Xu, 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nuclear pore inferred from sequence or structural similarity with EMBL:U41845 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nup50 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Aats-ile anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Aprt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Arc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Arf79F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Bub1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG10688 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG11060 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG11191 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG12163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG1416 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG14480 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG1516 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG17259 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG17765 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG2051 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG2789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG30447 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Nup50 - CG30491 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG4164 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG4858 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG6617 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG7609 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG7911 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG8010 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - CG8258 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Cct5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Cnx99A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Cyp12d1-p anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Dref anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - EfTuM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Fs(2)Ket anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Gmd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Hel25E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - ImpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Int6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Jon65Ai anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Kap-α3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Nedd4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Nup107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Nup153 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Nup50 - Nup160 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Nup37 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Nup44A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Pen anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Nup50 - R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rab1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rab2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rab5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - RpS29 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rpt2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Rpt6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Sec13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Sk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Sucb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Tapδ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Usp7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - Vps28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - atms anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - cher anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - cpb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - l(1)G0320 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - mt:CoII anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - skap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nup50 - ssp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nup50 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61640 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033264 DroID - A comprehensive database of gene and protein interactions. • FBgn0033264 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35784
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62V92P Dipteran inclusive ortholog search EOG66Q72Q Insect inclusive ortholog search EOG6XGXVJ Arthropod inclusive ortholog search EOG6XGXFH Metazoa inclusive ortholog search EOG6N3CGD
Orthologs in Drosophila Species (EOG62V92P)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nup50     Drosophila simulans   Dsim\GD10543 Y   Drosophila sechellia   Dsec\GM21009 Y   Drosophila erecta   Dere\GG23336 Y   Drosophila yakuba   Dyak\GE19178 Y   Drosophila ananassae   Dana\GF13664 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA23660     Drosophila pseudoobscura pseudoobscura   Dpse\GA15274 Y   Drosophila persimilis   Dper\GL17105 Y   Drosophila persimilis   Dper\GL14673     Drosophila willistoni   Dwil\GK23278 Y   Drosophila mojavensis   Dmoj\GI19257 Y   Drosophila grimshawi   Dgri\GH21906 Y
Orthologs in non-Drosophila Dipterans (EOG66Q72Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005635   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017037   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012737   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ019711   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015871   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017786   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ020181
Orthologs in non-Dipteran Insects (EOG6XGXVJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\NUP50   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009490   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10928   Atta cephalotes Leafcutter ant  Acep\ACEP20110   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13005   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15766   Linepithema humile Argentine ant  Lhum\LH19025   Pogonomyrmex barbatus Red harvester ant  Pbar\PB26055   Solenopsis invicta Red fire ant  Sinv\SINV11287   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006360   Bombyx mori Silkmoth  Bmor\BGIBMGA000995   Pediculus humanus Human body louse  Phum\PHUM205450   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07383
Orthologs in non-Insect Arthropods (EOG6XGXFH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\nisZ   Ixodes scapularis Deer tick  Isca\ISCW002462
Orthologs in non-Arthropod Metazoa (EOG6N3CGD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\npp-16   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009532gn   Danio rerio Zebrafish  Drer\nup50   Xenopus tropicalis Western clawed frog  Xtro\nup50   Gallus gallus Domestic chicken  Ggal\NUP50   Mus musculus House mouse  Mmus\Nup50   Mus musculus House mouse  Mmus\1700123L14Rik   Rattus norvegicus Norway rat  Rnor\Npap60   Rattus norvegicus Norway rat  Rnor\Npap60   Homo sapiens Human  Hsap\NUP50
Human Orthologs (1)
Gene OMIM HGNC Hsap\NUP50
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10543 Drosophila sechellia Dsec\GM21009 Drosophila erecta Dere\GG23336 Drosophila yakuba Dyak\GE19178 Drosophila ananassae Dana\GF13664 Drosophila pseudoobscura pseudoobscura Dpse\GA15274 Drosophila persimilis Dper\GL17105 Drosophila willistoni Dwil\GK23278 Drosophila virilis Dvir\GJ22132 Drosophila mojavensis Dmoj\GI19257 Drosophila grimshawi Dgri\GH21906
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	34580 y1 sc* v1; P{TRiP.HMS01054}attP2 15198 y1; P{SUPor-P}Nup50KG09557; ry506
Harvard	- PBac{RB}Nup50e02135
VDRC	v100564 P{KK108150}VIE-260B v20824 w1118; P{GD9739}v20824
Genomic Clones ( 2 )
	BACR09N11 BACR38O04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 64 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD27030
Other clones	DPiM_LD27030
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT13875 AT19010 AT20207 GH07225 GH14738 GH19975 GM05423 GM16888 LD07459 LD12553 LD12780 LD12840 LD13707 LD14861 LD23834 LD24292 LD24678 LD25890 LD26373 LD45194 LD46493 RE02048 RE18857 RE19443 RE19645 RE31915 RE35495 RE36895 RE43455 RE43520 RE43554 RE43560 RE43583 RE43861 RE45626 RE46454 RE54541 RE57888 RE60495 RE60663 RE61092 RE66191 RE72405
Other clones	64378 119531 158699 176079 562170 CK01.13B.A2 Dmel_dig_004825_0691_1904 Dmel_dig_010671_0586_3105 EK165864 EK179409 EK261636 EK280831 EN04512 EN13840 IP13892 IP13992 LD21185 LD26583 UUGC0703
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033264 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35784
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nup50 CG2158 (Gawlinski et al., 2007)
Source for database merge of
Additional comments
Other Comments
	S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136511 RefSeq (Proteins) • NP_610355 DDBJ / EMBL / GenBank • AA941675 AAF59131 AAL48017 AW942867 AY070546 CZ479960 Entrez Gene - A searchable database of RefSeq genes. • 35784 UniProtKB/TrEMBL • Q7K0D8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ran binding domain (IPR000156) Pleckstrin homology-like domain (IPR011993) Nuclear pore complex, NUP2/50/61 (IPR015007) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD27030
Linkouts
	BioGRID - A database of protein and genetic interactions • 61640 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033264 DroID - A comprehensive database of gene and protein interactions. • FBgn0033264 DRSC - Results from RNAi screens. • FBgn0033264 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033264 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2158-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033264 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35784 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35784 modMine - Data generated by the modENCODE project. • FBgn0033264
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG2158 (Gawlinski et al., 2007, Stroschein-Stevenson et al., 2006, Stuart et al., 2007, Doheny et al., 2008, Keleman et al., 2009.8.5, Mummery-Widmer et al., 2009, Ni et al., 2010.12.1, Sabri et al., 2007) Nup50 (Mummery-Widmer et al., 2009, Kalverda et al., 2010, Gawlinski et al., 2007, Vaquerizas et al., 2010, Neumann et al., 2010) nup50 (Chen and Xu, 2010)
Name Synonym	Nuclear pore protein 50 (Gawlinski et al., 2007) Nucleoporin 50   Nucleoporin 50kD
Secondary FlyBase IDs
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 9 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 3 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Zhan et al., 2013, PLoS ONE 8(2): e57214 Identification of Genetic Modifiers of TDP-43 Neurotoxicity in Drosophila. [FBrf0220979] Crawford et al., 2012, Cell Death Differ. 19(12): 2040--2048 Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. [FBrf0220233] Show all research papers ...