• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\CG8258

General Information
Symbol	Dmel\CG8258	Species	D. melanogaster
Name		Annotation symbol	CG8258
Feature type	protein_coding_gene	FlyBase ID	FBgn0033342
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	44F5-44F5	Sequence location	2R:4,792,349..4,794,495 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG8258 (FBgn0033342). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled. There is experimental evidence that it is involved in the biological process: mitotic spindle organization; cytoplasmic microtubule organization. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Chaperone, tailless complex polypeptide 1; Chaperonin Cpn60/TCP-1; Chaperonin TCP-1, conserved site; T-complex protein 1, theta subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during early larval stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, larval salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634700_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8258. Gene sequence location is 2R:4792349..4794495.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44F5-44F5   Limits computationally determined from genome sequence between P{lacW}Dmnk16109&P{lacW}Gγ1k08017 and P{lacW}babok16912
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG8258 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG8258-RA FBtr0088683  NM_136574   1904   546
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG8258-PA   FBpp0087764   59.4   546   4.94     NP_610418   AAF59029
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Chaperonin TCP-1, conserved site (IPR002194) Chaperonin Cpn60/TCP-1 (IPR002423) T-complex protein 1, theta subunit (IPR012721) Chaperone tailless complex polypeptide 1 (TCP-1) (IPR017998) TCP-1-like chaperonin intermediate domain (IPR027410) GroEL-like apical domain (IPR027409) GroEL-like equatorial domain (IPR027413)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440547   AA567120   AA695014   AA696153   AA696466   AA699084   AA735769   AA803375   AA803873   AA803874   AA820661   AA821067   AA821204   AA940913   AA941235   AE013599 AAF59029   AI237974   AI258983   AI259370   AI295316   AI296576   AI455490   AI456149   AI456779   AI516441   AI518101   AI520360   AI533197   AY058545 AAL13774   BF494477   BF504865   BF506254   BG632703   BG640089   BI166913   BI168597   BI214102   BI214880   BI231682   BI233121   BI235083   BI351958   BI352203   BI353197   BI353391   BI353663   BI354923   BI355520   BI357479   BI357530   BI364526   BI366526   BI367205   BI368898   BI372131   BI565941   BI574359   BI574506   BI574771   BI585437   BI587356   BI587387   BI607973   BI612166   BI613175   BI613683   BI618625   BI619579   BI625662   BI627009   BI628687   BI632373   BI633318   BI634559   BI634831   BI635098   BI635584   BI635732   BI636008   BI636083   BI636088   BI639589   BI640861   BI641218   BP548086   BP554470   BP554471   CK132323   CO187368   CO187994   CO262647   CO267479   CO296562   CO329235   CO336231   EC067302   EC070608   EC079024   EC089496   EC199190   EC205027   EC206002   EC206297   EC227817   EC229646   EC264170   EL878250   EL883564   EV596602
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K3J0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anal pad primordium   (Fisher et al., 2012) Comment:reported as anal pad specific anlage anterior midgut primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) Malpighian tubule primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, larval salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult testis, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1166.9 Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland    1100.4 Larval Trachea    1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary    1532.1 Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1166.9) Larval Midgut  (560.3) Larval Hindgut  (998.5) Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland  (1100.4) Larval Trachea  (1077.75) Larval Carcass  (949.875) Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop  (615.6) Adult Midgut  (611.8) Adult Hindgut  536.8 Adult Malpighian Tubules  (542.2) Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary  (1532.1) Adult Testis    422.8 Adult Male Accessory Gland  (661.8) Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1166.9) Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland  (1100.4) Larval Trachea  1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary  (1532.1) Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1166.9 Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland    1100.4 Larval Trachea    1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary    1532.1 Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1166.9 Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland    1100.4 Larval Trachea    1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary    1532.1 Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1166.9) Larval Midgut  560.3 Larval Hindgut  (998.5) Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland  (1100.4) Larval Trachea  (1077.75) Larval Carcass  (949.875) Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop  615.6 Adult Midgut  611.8 Adult Hindgut  536.8 Adult Malpighian Tubules  542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary  (1532.1) Adult Testis    422.8 Adult Male Accessory Gland  661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1166.9 Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland  1100.4 Larval Trachea  1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary  1532.1 Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1166.9 Larval Midgut    560.3 Larval Hindgut    998.5 Larval Malpighian Tubules    346.3 Larval Fat Body    293.7 Larval Salivary Gland    1100.4 Larval Trachea    1077.75 Larval Carcass    949.875 Adult Head    347 Adult Eye    236.7 Adult Brain    324 Adult Thoracic-Abdominal Ganglion    401.9 Adult Crop    615.6 Adult Midgut    611.8 Adult Hindgut    536.8 Adult Malpighian Tubules    542.2 Adult Fat Body    339.6 Adult Salivary Gland    375.3 Adult Heart    281.6 Adult VirginFemale Spermatheca    279.4 Adult InseminatedFemale Spermatheca    371.2 Adult Ovary    1532.1 Adult Testis    422.8 Adult Male Accessory Gland    661.8 Adult Carcass    337.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    347   NA  Eye    236.7   NA  Brain    324   1166.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    401.9   NA  Crop    615.6   560.3  Midgut    611.8   998.5  Hindgut    536.8   346.3  Malpighian Tubules    542.2   293.7  Fat Body    339.6   1100.4  Salivary Gland    375.3   NA  Heart    281.6   1077.75  Trachea    NA   NA  VirginFemale Spermatheca    279.4   NA  InseminatedFemale Spermatheca    371.2   NA  Ovary    1532.1   NA  Testis    422.8   NA  Male Accessory Gland    661.8   949.875  Carcass    337.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female    150 ovary, mated 4-day female    130 testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (104) central nervous system, larvae L3  (68) central nervous system, pupae P8  26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering  (30) digestive system, 1-day adult  (28) digestive system, 4-day adult  (38) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8  (36) carcass, larvae L3 wandering  (67) carcass, 1-day adult    14 carcass, 4-day adult  (27) carcass, 20-day adult    22 ovary, virgin 4-day female  (150) ovary, mated 4-day female  (130) testis, mated 4-day male  (52) accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female  (150) ovary, mated 4-day female  (130) testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female    150 ovary, mated 4-day female    130 testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female    150 ovary, mated 4-day female    130 testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (104) central nervous system, larvae L3  (68) central nervous system, pupae P8  26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering  30 digestive system, 1-day adult  28 digestive system, 4-day adult  (38) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8  (36) carcass, larvae L3 wandering  (67) carcass, 1-day adult    14 carcass, 4-day adult  27 carcass, 20-day adult    22 ovary, virgin 4-day female  (150) ovary, mated 4-day female  (130) testis, mated 4-day male  (52) accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female  (150) ovary, mated 4-day female  130 testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    104 central nervous system, larvae L3    68 central nervous system, pupae P8    26 head, virgin 1-day female    12 head, virgin 4-day female    10 head, virgin 20-day female    18 head, mated 1-day female    9 head, mated 4-day female    23 head, mated 20-day female    15 head, mated 1-day male    11 head, mated 4-day male    14 head, mated 20-day male    22 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    28 digestive system, 4-day adult    38 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    17 fat body, pupae P8    36 carcass, larvae L3 wandering    67 carcass, 1-day adult    14 carcass, 4-day adult    27 carcass, 20-day adult    22 ovary, virgin 4-day female    150 ovary, mated 4-day female    130 testis, mated 4-day male    52 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during early larval stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    306 embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr    118 embryo 08-10hr    129 embryo 10-12hr    179 embryo 12-14hr    116 embryo 14-16hr    114 embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1    208 larva L2    175 larva L3 12hr old    135 larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new    104 white prepupae 12hr    104 white prepupae 24hr    119 pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day    211 adult female 05day    281 adult female 30day    288 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (306) embryo 02-04hr  (54) embryo 04-06hr  (80) embryo 06-08hr  (118) embryo 08-10hr  (129) embryo 10-12hr  (179) embryo 12-14hr  (116) embryo 14-16hr  (114) embryo 16-18hr  (76) embryo 18-20hr  (67) embryo 20-22hr  (77) embryo 22-24hr  (66) larva L1  (208) larva L2  (175) larva L3 12hr old  (135) larva L3 puffstage 1-2  (67) larva L3 puffstage 3-6  (95) larva L3 puffstage 7-9  (99) white prepupae new  (104) white prepupae 12hr  (104) white prepupae 24hr  (119) pupae 2d postWPP  (80) pupae 3d postWPP  (68) pupae 4d postWPP  (63) adult male 01day  (44) adult male 05day  (56) adult male 30day  (64) adult female 01day  (211) adult female 05day  (281) adult female 30day  (288) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (306) embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr  (118) embryo 08-10hr  (129) embryo 10-12hr  (179) embryo 12-14hr  (116) embryo 14-16hr  (114) embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1  (208) larva L2  (175) larva L3 12hr old  (135) larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new  104 white prepupae 12hr  104 white prepupae 24hr  (119) pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day  (211) adult female 05day  (281) adult female 30day  (288) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    306 embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr    118 embryo 08-10hr    129 embryo 10-12hr    179 embryo 12-14hr    116 embryo 14-16hr    114 embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1    208 larva L2    175 larva L3 12hr old    135 larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new    104 white prepupae 12hr    104 white prepupae 24hr    119 pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day    211 adult female 05day    281 adult female 30day    288 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    306 embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr    118 embryo 08-10hr    129 embryo 10-12hr    179 embryo 12-14hr    116 embryo 14-16hr    114 embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1    208 larva L2    175 larva L3 12hr old    135 larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new    104 white prepupae 12hr    104 white prepupae 24hr    119 pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day    211 adult female 05day    281 adult female 30day    288 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (306) embryo 02-04hr  (54) embryo 04-06hr  (80) embryo 06-08hr  (118) embryo 08-10hr  (129) embryo 10-12hr  (179) embryo 12-14hr  (116) embryo 14-16hr  (114) embryo 16-18hr  (76) embryo 18-20hr  (67) embryo 20-22hr  (77) embryo 22-24hr  (66) larva L1  (208) larva L2  (175) larva L3 12hr old  (135) larva L3 puffstage 1-2  (67) larva L3 puffstage 3-6  (95) larva L3 puffstage 7-9  (99) white prepupae new  (104) white prepupae 12hr  (104) white prepupae 24hr  (119) pupae 2d postWPP  (80) pupae 3d postWPP  (68) pupae 4d postWPP  (63) adult male 01day  (44) adult male 05day  (56) adult male 30day  (64) adult female 01day  (211) adult female 05day  (281) adult female 30day  (288) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (306) embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr  118 embryo 08-10hr  129 embryo 10-12hr  (179) embryo 12-14hr  116 embryo 14-16hr  114 embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1  (208) larva L2  (175) larva L3 12hr old  135 larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new  104 white prepupae 12hr  104 white prepupae 24hr  119 pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day  (211) adult female 05day  (281) adult female 30day  (288) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    306 embryo 02-04hr    54 embryo 04-06hr    80 embryo 06-08hr    118 embryo 08-10hr    129 embryo 10-12hr    179 embryo 12-14hr    116 embryo 14-16hr    114 embryo 16-18hr    76 embryo 18-20hr    67 embryo 20-22hr    77 embryo 22-24hr    66 larva L1    208 larva L2    175 larva L3 12hr old    135 larva L3 puffstage 1-2    67 larva L3 puffstage 3-6    95 larva L3 puffstage 7-9    99 white prepupae new    104 white prepupae 12hr    104 white prepupae 24hr    119 pupae 2d postWPP    80 pupae 3d postWPP    68 pupae 4d postWPP    63 adult male 01day    44 adult male 05day    56 adult male 30day    64 adult female 01day    211 adult female 05day    281 adult female 30day    288 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    185 Schneider line 2 Sg4    240 embryonic 1182-4H    141 embryonic GM2    158 embryonic Kc167    76 embryonic S1    292 embryonic S3    277 leg disc CME L1    174 wing disc CME-W2    167 wing disc ML-DmD8    142 wing disc ML-DmD9    133 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    218 wing disc ML-DmD32    102 haltere disc ML-DmD17-c3    225 eye-antennal disc ML-DmD11    127 antennal disc ML-DmD20-c5    230 mixed discs ML-DmD4-c1    149 CNS ML-DmBG1-c1    175 CNS ML-DmBG2-c2    199 tumorous blood cells mbn2    111 ovary fGS/OSS    119 ovary OSC    102 ovary OSS    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (185) Schneider line 2 Sg4  (240) embryonic 1182-4H  (141) embryonic GM2  (158) embryonic Kc167  (76) embryonic S1  (292) embryonic S3  (277) leg disc CME L1  (174) wing disc CME-W2  (167) wing disc ML-DmD8  (142) wing disc ML-DmD9  (133) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  (218) wing disc ML-DmD32  (102) haltere disc ML-DmD17-c3  (225) eye-antennal disc ML-DmD11  (127) antennal disc ML-DmD20-c5  (230) mixed discs ML-DmD4-c1  (149) CNS ML-DmBG1-c1  (175) CNS ML-DmBG2-c2  (199) tumorous blood cells mbn2  (111) ovary fGS/OSS  (119) ovary OSC  (102) ovary OSS  (62) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (185) Schneider line 2 Sg4  (240) embryonic 1182-4H  (141) embryonic GM2  (158) embryonic Kc167    76 embryonic S1  (292) embryonic S3  (277) leg disc CME L1  (174) wing disc CME-W2  (167) wing disc ML-DmD8  (142) wing disc ML-DmD9  (133) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  (218) wing disc ML-DmD32  102 haltere disc ML-DmD17-c3  (225) eye-antennal disc ML-DmD11  (127) antennal disc ML-DmD20-c5  (230) mixed discs ML-DmD4-c1  (149) CNS ML-DmBG1-c1  (175) CNS ML-DmBG2-c2  (199) tumorous blood cells mbn2  (111) ovary fGS/OSS  (119) ovary OSC  102 ovary OSS    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    185 Schneider line 2 Sg4    240 embryonic 1182-4H    141 embryonic GM2    158 embryonic Kc167    76 embryonic S1    292 embryonic S3    277 leg disc CME L1    174 wing disc CME-W2    167 wing disc ML-DmD8    142 wing disc ML-DmD9    133 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    218 wing disc ML-DmD32    102 haltere disc ML-DmD17-c3    225 eye-antennal disc ML-DmD11    127 antennal disc ML-DmD20-c5    230 mixed discs ML-DmD4-c1    149 CNS ML-DmBG1-c1    175 CNS ML-DmBG2-c2    199 tumorous blood cells mbn2    111 ovary fGS/OSS    119 ovary OSC    102 ovary OSS    62 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    185 Schneider line 2 Sg4    240 embryonic 1182-4H    141 embryonic GM2    158 embryonic Kc167    76 embryonic S1    292 embryonic S3    277 leg disc CME L1    174 wing disc CME-W2    167 wing disc ML-DmD8    142 wing disc ML-DmD9    133 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    218 wing disc ML-DmD32    102 haltere disc ML-DmD17-c3    225 eye-antennal disc ML-DmD11    127 antennal disc ML-DmD20-c5    230 mixed discs ML-DmD4-c1    149 CNS ML-DmBG1-c1    175 CNS ML-DmBG2-c2    199 tumorous blood cells mbn2    111 ovary fGS/OSS    119 ovary OSC    102 ovary OSS    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (185) Schneider line 2 Sg4  (240) embryonic 1182-4H  (141) embryonic GM2  (158) embryonic Kc167  (76) embryonic S1  (292) embryonic S3  (277) leg disc CME L1  (174) wing disc CME-W2  (167) wing disc ML-DmD8  (142) wing disc ML-DmD9  (133) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  (218) wing disc ML-DmD32  (102) haltere disc ML-DmD17-c3  (225) eye-antennal disc ML-DmD11  (127) antennal disc ML-DmD20-c5  (230) mixed discs ML-DmD4-c1  (149) CNS ML-DmBG1-c1  (175) CNS ML-DmBG2-c2  (199) tumorous blood cells mbn2  (111) ovary fGS/OSS  (119) ovary OSC  (102) ovary OSS  (62) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (185) Schneider line 2 Sg4  (240) embryonic 1182-4H  141 embryonic GM2  (158) embryonic Kc167    76 embryonic S1  (292) embryonic S3  (277) leg disc CME L1  (174) wing disc CME-W2  (167) wing disc ML-DmD8  142 wing disc ML-DmD9  133 wing disc ML-DmD16-c3  128 wing disc ML-DmD21  (218) wing disc ML-DmD32  102 haltere disc ML-DmD17-c3  (225) eye-antennal disc ML-DmD11  127 antennal disc ML-DmD20-c5  (230) mixed discs ML-DmD4-c1  (149) CNS ML-DmBG1-c1  (175) CNS ML-DmBG2-c2  (199) tumorous blood cells mbn2  111 ovary fGS/OSS  119 ovary OSC  102 ovary OSS    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    185 Schneider line 2 Sg4    240 embryonic 1182-4H    141 embryonic GM2    158 embryonic Kc167    76 embryonic S1    292 embryonic S3    277 leg disc CME L1    174 wing disc CME-W2    167 wing disc ML-DmD8    142 wing disc ML-DmD9    133 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    218 wing disc ML-DmD32    102 haltere disc ML-DmD17-c3    225 eye-antennal disc ML-DmD11    127 antennal disc ML-DmD20-c5    230 mixed discs ML-DmD4-c1    149 CNS ML-DmBG1-c1    175 CNS ML-DmBG2-c2    199 tumorous blood cells mbn2    111 ovary fGS/OSS    119 ovary OSC    102 ovary OSS    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult    106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    137 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    159 Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (34) heat shock, 4-day adult  (106) Cadmium 50 mM 6 hrs, larvae L3  (53) Cadmium 50 mM 12 hrs, larvae L3  (41) Cadmium 50 mM 48 hrs, 4-day adult  (68) Cadmium 100 mM 48 hrs, 4-day adult  (84) Copper 0.5 mM 12 hrs, larvae L3  (33) Copper 15 mM 48 hrs, 4-day adult  (137) Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3  (159) Ethanol 5% 3 hrs, larvae L3  (46) Ethanol 10% 3 hrs, larvae L3  (56) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (34) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (88) Caffeine 25 mg/ml 48 hrs, 4-day adult  (35) Paraquat 5 mM 48 hrs, 4-day adult  (86) Paraquat 10 mM 48 hrs, 4-day adult  (104) Rotenone 2 μg 12 hrs, larvae L3  (64) Rotenone 8 μg 12 hrs, larvae L3  (80) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult  106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult  (137) Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3  (159) Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult  104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult    106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    137 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    159 Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult    106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    137 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    159 Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (34) heat shock, 4-day adult  (106) Cadmium 50 mM 6 hrs, larvae L3  (53) Cadmium 50 mM 12 hrs, larvae L3  (41) Cadmium 50 mM 48 hrs, 4-day adult  (68) Cadmium 100 mM 48 hrs, 4-day adult  (84) Copper 0.5 mM 12 hrs, larvae L3  (33) Copper 15 mM 48 hrs, 4-day adult  (137) Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3  (159) Ethanol 5% 3 hrs, larvae L3  (46) Ethanol 10% 3 hrs, larvae L3  (56) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (34) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (88) Caffeine 25 mg/ml 48 hrs, 4-day adult  (35) Paraquat 5 mM 48 hrs, 4-day adult  (86) Paraquat 10 mM 48 hrs, 4-day adult  (104) Rotenone 2 μg 12 hrs, larvae L3  (64) Rotenone 8 μg 12 hrs, larvae L3  (80) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult  106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult  137 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3  (159) Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult  104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    34 heat shock, 4-day adult    106 Cadmium 50 mM 6 hrs, larvae L3    53 Cadmium 50 mM 12 hrs, larvae L3    41 Cadmium 50 mM 48 hrs, 4-day adult    68 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    137 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    159 Ethanol 5% 3 hrs, larvae L3    46 Ethanol 10% 3 hrs, larvae L3    56 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    88 Caffeine 25 mg/ml 48 hrs, 4-day adult    35 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    104 Rotenone 2 μg 12 hrs, larvae L3    64 Rotenone 8 μg 12 hrs, larvae L3    80 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • LD24495 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8258-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG8258 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG8258 Class Mutagen Stocks Known lesion CG8258CPTI003689 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG8258 Class Mutagen Stocks Known lesion CG8258GD13863 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG8258KK100186 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG8258NIG.8258R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel8048 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13863} NA P{KK100186} NA P{NIG.8258R} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}l(2)0399603996 No miscellaneous insertions PBac{768.FSVS-0}CG8258CPTI003689 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cytoplasmic microtubule organization inferred from mutant phenotype (Hughes et al., 2008) mitotic spindle organization inferred from mutant phenotype (Hughes et al., 2008)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATPase activity, coupled inferred from sequence or structural similarity with UniProtKB:P50990 (FlyBase, 1992-) ATP binding inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR002423, InterPro:IPR012721 (FlyBase Curators et al., 2004-) unfolded protein binding inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR012721 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein folding inferred from sequence or structural similarity with UniProtKB:P50990 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
chaperonin-containing T-complex inferred from sequence or structural similarity with UniProtKB:P50990 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG8258 - 5-HT7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Aats-glupro experimental knowledge based (Guruharsha et al., 2011) CG8258 - Aats-his anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ada anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Arp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - BI-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG10425 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG10859 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG10996 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG1105 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG1109 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG11534 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG11876 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG12084 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG12123 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG12262 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG12264 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG12289 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG12333 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG13539 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG1371 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG14024 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG14195 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG1444 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG14482 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG14722 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG14762 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG15625 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG15643 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG17259 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG17337 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG1907 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG1909 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG2064 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG2183 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG2789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG2812 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG2931 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG3008 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG31522 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG31523 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG31637 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG3303 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG3313 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - CG33303 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG3523 experimental knowledge based (Guruharsha et al., 2011) CG8258 - CG3530 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG3570 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - CG4511 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG4563 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG4764 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5027 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5525 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - CG5623 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5646 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5660 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5828 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG5961 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG6073 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG6321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG7033 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - CG7609 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG8602 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG9062 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG9231 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG9547 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CG9945 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CR18854 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - CSN4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Cct5 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - Cctγ anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - Chc experimental knowledge based (Guruharsha et al., 2011) CG8258 - CkIIβ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Cnx99A experimental knowledge based (Guruharsha et al., 2011) CG8258 - Cyp12d1-p experimental knowledge based (Guruharsha et al., 2011) CG8258 - Cyp6a21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Cyp9b2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ent2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Esp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Fatp experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - Fmo-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Gfat2 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Gie anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Glt experimental knowledge based (Guruharsha et al., 2011) CG8258 - Gmd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Gp210 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Gp93 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Gyk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Hdac3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Hip1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Iap2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ilk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Kap-α3 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Kr-h2 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Lis-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - MESK2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - MRP experimental knowledge based (Guruharsha et al., 2011) CG8258 - Mec2 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Mpcp experimental knowledge based (Guruharsha et al., 2011) CG8258 - ND42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ndfip anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Nle anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Nup50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ocrl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Odc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Ost48 experimental knowledge based (Guruharsha et al., 2011) CG8258 - OstΔ experimental knowledge based (Guruharsha et al., 2011) CG8258 - Past1 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Pi3K68D experimental knowledge based (Guruharsha et al., 2011) CG8258 - Pis anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Pp1-87B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Pp2A-29B experimental knowledge based (Guruharsha et al., 2011) CG8258 - Psa experimental knowledge based (Guruharsha et al., 2011) CG8258 - Rab18 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Rab7 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Ranbp9 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Rheb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Rpd3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Rpn1 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Rpp30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Rpt1 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Rpt6 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Sec61α experimental knowledge based (Guruharsha et al., 2011) CG8258 - Snap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Sucb experimental knowledge based (Guruharsha et al., 2011) CG8258 - Syb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - T-cp1 experimental knowledge based (Guruharsha et al., 2011) CG8258 - TER94 experimental knowledge based (Guruharsha et al., 2011) CG8258 - Tango4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Tap42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - Tcp-1η experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Tg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Tsp29Fb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - Vps4 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - alrm anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - atms anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - boca experimental knowledge based (Guruharsha et al., 2011) CG8258 - bor experimental knowledge based (Guruharsha et al., 2011) CG8258 - bsk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - endoB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - fax experimental knowledge based (Guruharsha et al., 2011) CG8258 - frc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - fzr2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - fzy anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - grk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - gro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - ine anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - l(1)G0156 experimental knowledge based (Guruharsha et al., 2011) CG8258 - l(2)dtl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - ldbr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - lic anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - mio anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - shd experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG8258 - shrb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - skap experimental knowledge based (Guruharsha et al., 2011) CG8258 - stas anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - tomboy20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - tws anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - viaf anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG8258 - wal experimental knowledge based (Guruharsha et al., 2011) CG8258 - wds anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - βCop experimental knowledge based (Guruharsha et al., 2011) CG8258 - βggt-II anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - γTub23C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8258 - γTub37C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG8258 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033342 DroID - A comprehensive database of gene and protein interactions. • FBgn0033342 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35882
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6JM88D Dipteran inclusive ortholog search EOG66MBSW Insect inclusive ortholog search EOG6VT4SJ Arthropod inclusive ortholog search EOG6NZS8K Metazoa inclusive ortholog search EOG6P8TBB
Orthologs in Drosophila Species (EOG6JM88D)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG8258     Drosophila simulans   Dsim\GD10130 Y   Drosophila sechellia   Dsec\GM20657 Y   Drosophila erecta   Dere\GG10613 Y   Drosophila yakuba   Dyak\GE22743 Y   Drosophila ananassae   Dana\GF12562 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20937 Y   Drosophila persimilis   Dper\GL10409 Y   Drosophila willistoni   Dwil\GK21949 Y   Drosophila virilis   Dvir\GJ21056 Y   Drosophila mojavensis   Dmoj\GI20616 Y   Drosophila grimshawi   Dgri\GH22689 Y
Orthologs in non-Drosophila Dipterans (EOG66MBSW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012746   Anopheles gambiae Malaria mosquito  Agam\AGAP010588   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005864
Orthologs in non-Dipteran Insects (EOG6VT4SJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC551436   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001149   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12034   Atta cephalotes Leafcutter ant  Acep\ACEP10148   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18831   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15131   Linepithema humile Argentine ant  Lhum\LH14156   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24534   Solenopsis invicta Red fire ant  Sinv\SINV18575   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002247   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005867   Bombyx mori Silkmoth  Bmor\BGIBMGA011508   Pediculus humanus Human body louse  Phum\PHUM212280   Tribolium castaneum Red flour beetle  Tcas\TC001073
Orthologs in non-Insect Arthropods (EOG6NZS8K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COL17A1   Ixodes scapularis Deer tick  Isca\ISCW012934
Orthologs in non-Arthropod Metazoa (EOG6P8TBB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\cct-8   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015592gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006047gn   Danio rerio Zebrafish  Drer\cct8   Xenopus tropicalis Western clawed frog  Xtro\cct8   Gallus gallus Domestic chicken  Ggal\CCT8   Mus musculus House mouse  Mmus\Cct8   Rattus norvegicus Norway rat  Rnor\Cct8   Homo sapiens Human  Hsap\CCT8
Human Orthologs (1)
Gene OMIM HGNC Hsap\CCT8
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10130 Drosophila sechellia Dsec\GM20657 Drosophila erecta Dere\GG10613 Drosophila yakuba Dyak\GE22743 Drosophila ananassae Dana\GF12562 Drosophila pseudoobscura pseudoobscura Dpse\GA20937 Drosophila persimilis Dper\GL10409 Drosophila willistoni Dwil\GK21949 Drosophila virilis Dvir\GJ21056 Drosophila mojavensis Dmoj\GI20616 Drosophila grimshawi Dgri\GH22689
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v103905 P{KK100186}VIE-260B v45790 w1118; P{GD13863}v45790 v45789 w1118; P{GD13863}v45789/TM3
Genomic Clones ( 1 )
	BACR02C23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 106 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD24495
Other clones	DPiM_LD24495
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03089 AT06919 AT08683 GH12587 GH13193 GM01141 GM01940 GM05185 GM06151 GM07736 GM09818 GM10744 GM14502 GM14503 GM14864 GM17007 GM18310 GM22634 GM23438 GM24625 GM28901 GM31215 HL07439 LD15358 LD23245 LD25265 LD36010 LD36906 LD37521 LD40494 LD42312 LP02294 LP02767 LP08912 LP10533 LP13762 RE07159 RE09402 RE19812 RE21026 RE22991 RE29406 RE30888 RE43982 RE44044 RE49673 RE52081 RE52916 RE54957 RE59012 RH12086 RH23323 RH24410 RH25012 RH27005 RH28013 RH28052 RH34777 RH38739 RH39073 RH40572 RH42273 RH42890 RH49493 RH50643 RH57195 RH66130 RH68184 SD04944 SD11037 SD16338 SD16974 SD17181 SD17565 SD17651 SD17656 SD22218 SD23873 SD24436 SD26138 SD27379
Other clones	38234 162939 190041 191226 193656 193854 557129 CK01.7D.C2 CK01.23B.E5 CK01.41B.F8 CK01.102A.C3 Dmel_neb_003688_0937_0701 EK028615 EK035556 EK086010 EK150439 EK214107 EK292325 EN12118 FGH123H03 FGM230A09 IP08134 IP13223
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35882
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	"l(2)03996" probably corresponds to "CG8258"; the P{PZ} insertion in the "l(2)0399603996" allele maps 7bp upstream of the 5' end of the CT8297 transcript of "CG8258". (Misra, 2000.8.25)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136574 RefSeq (Proteins) • NP_610418 DDBJ / EMBL / GenBank • AA820661 AAF59029 AAL13774 AI455490 AY058545 Entrez Gene - A searchable database of RefSeq genes. • 35882 UniProtKB/TrEMBL • Q7K3J0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Chaperonin TCP-1, conserved site (IPR002194) Chaperonin Cpn60/TCP-1 (IPR002423) T-complex protein 1, theta subunit (IPR012721) Chaperone tailless complex polypeptide 1 (TCP-1) (IPR017998) TCP-1-like chaperonin intermediate domain (IPR027410) GroEL-like apical domain (IPR027409) GroEL-like equatorial domain (IPR027413) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD24495
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033342 DroID - A comprehensive database of gene and protein interactions. • FBgn0033342 DRSC - Results from RNAi screens. • FBgn0033342 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8258-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35882 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35882 modMine - Data generated by the modENCODE project. • FBgn0033342
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CCT8 (Monzo et al., 2010) CG8258 (Daneshvar et al., 2013, Hughes et al., 2008, Keleman et al., 2009.8.5, Chen et al., 2008, Monzo et al., 2010, Neal et al., 2006, Cammarato et al., 2011, Neumüller et al., 2011, Pflieger et al., 2008, Japanese National Institute of Genetics, 2012.5.21) Q7K3J0_DROME (Fukuyama et al., 2012) Tcp1-theta (Fisher et al., 2008) TCP-1θ (Monzo et al., 2010)
Name Synonym
Secondary FlyBase IDs
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Daneshvar et al., 2013, Biol. Open 2(1): 1--9 MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila. [FBrf0220584] Fukuyama et al., 2012, J. Proteomics 75(15): 4610--4619 On-bead tryptic proteolysis: An attractive procedure for LC-MS/MS analysis of the Drosophila caspase 8 protein complex during immune response against bacteria. [FBrf0218992] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...