l(2)02353, dmmp2, Dm2-MMP
enzymes required for several stereotyped motor axon pathfinding decisions and essential for axon fasciculation - Mmp2 promotes dendrite reshaping through local degradation of the basement membrane - in trachea Mmp2 inhibits FGF morphogenetic function - Mmp2 is essential for wing imaginal disc:trachea association and air sac tubulogenesis
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
3.4 (sequence analysis)
None of the polypeptides share 100% sequence identity.
758 (aa); 89 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mmp2 using the Feature Mapper tool.
Mmp2 is widely expressed in the embryonic CNS. At stage 15, it is expressed in the midline glia and in approximately three other glial cells per hemisegment (as identified by repo expression, situated at the base of the motor nerve roots. These may correspond to the exit glia. Mmp2 is also expressed in a subset of neurons in the embyronic CNS including the tup-expressing neurons.
Mmp2 is expressed in many tissues from stage 10 including the mesoderm, stomatogastric nervous system, ectoderm, and the peripheral nervous system. Starting in stage 14, it is expressed in segmentally repeated cells in the developing central nervous system. It is expressed in gut constrictions in stages 15 and 16 and in the embryonic brain by stage 17. In wandering third instar larvae, Mmp2 is expressed widely in the wing disc, less in leg disc, and abundantly in the morphogenetic furrow and in developing ommatidia. Expression occurs in discrete foci throughout the ventral nerve cord and brain lobes and is strong in the optic lobe.
Mmp2 transcripts are detected in embryos, larvae, pupae, and adult males and females by RT-PCR. They are not detected by northern blot. They are observed by in situ hybridization in third instar larval imaginal discs and brain. Strong expression is seen in the eye disc behind the morphogenetic furrow and in the lamina.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Mmp2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Mmp2 CG1794
Source for merge of Mmp2 anon-WO0118547.84 was sequence comparison ( date:051113 ).
Mmp2 limits long-distance wg signaling in the germarium. Mmp2 protein cleaves dlp protein in cultured cells and is expressed in the apical cells (and a few anterior escort cells) of the germarium niche. Mmp2 may act to limit wg signaling in the the germarium by cleaving dlp protein near the wg source, resulting in relocation of dlp protein from the cell surface to intracellular vesicles, such that dlp can no longer contact wg protein and promote long-range wg signaling.
Mutants are pupal lethal and show defects in oogenesis.
Area matching Drosophila EST AI238523.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.