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Gene Dmel\Pal1

General Information
Symbol	Dmel\Pal1	Species	D. melanogaster
Name	Peptidyl-α-hydroxyglycine-α-amidating lyase 1	Annotation symbol	CG12130
Feature type	protein_coding_gene	FlyBase ID	FBgn0033466
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dPAL1
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	46C7-46C7	Sequence location	2R:5,847,054..5,851,358 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Peptidyl-α-hydroxyglycine-α-amidating lyase 1 is referred to in FlyBase by the symbol Dmel\Pal1 (CG12130, FBgn0033466). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: peptidylamidoglycolate lyase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: oxidation-reduction process; peptide metabolic process. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: NHL repeat; NHL repeat, subgroup; Peptidyl-glycine alpha-amidating monooxygenase; Six-bladed beta-propeller, TolB-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1636841_at partially aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG12130: 6 out of 14 probes fall into this exonic region. Gene sequence location is 2R:5847054..5851358.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Guenin et al., 2010, BMC Genomics 11: 47 Sequence features BKN21673 HFA06106
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46C7-46C7   Limits computationally determined from genome sequence between P{lacW}l(2)k08601k08601 and P{lacW}Adamk13906&P{EP}Pka-R2EP2162
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pal1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pal1-RA FBtr0088385  NM_136693   2621   541 Pal1-RB FBtr0273269  NM_001144158   2690   564
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pal1-PA   FBpp0087473   59.6   541   9.35     NP_610537   AAF58870 Pal1-PB   FBpp0271777   62.1   564   9.43     NP_001137630   ACL83084
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidyl-glycine alpha-amidating monooxygenase (IPR000720) NHL repeat, subgroup (IPR013017) Six-bladed beta-propeller, TolB-like (IPR011042) NHL repeat (IPR001258)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201267   AA263489   AA440802   AA441585   AA951352   AA978861   AA990839   AE013599 AAF58870   AE013599 ACL83084   AI106775   AI258972   AI259073   AI293800   AI455660   AI456796   AI518780   AI544217   AW943245   AX191631   AY051842 AAK93266   BF486480   BF486963   BF491392   BF495251   BF498823   BF498893   BG635152   BG635799   BG637232   BG638684   BI163797   BI173542   BI212514   BI212515   BI213572   BI239253   BI356635   BI357614   BI364299   BI365885   BI367466   BI367547   BI368156   BI569210   BI569211   BI577994   BI589881   BI590492   BI590596   BI593534   BI608662   BI608671   BI620791   BI640400   BT050472   CA807005   CK135987   CO187086   CO264789   CO273512   CO290196   CO304325   CO324877   EC058860   EC063409   EC079897   EC083883   EC202670   EC203543   EC204372   EC213542   EC216409   EC250709   ES695825   EV590224   GH942155   GH942684   GH942761   GH943564   GH943777   GH944708   GH945782   GH945999   GH946134   GH946881   GH949413   GH951661   GH953214   GH954391   GH959973   GH964652   GH967988   GH968086   GH968698   GH969942   GH972007   GH972019   GH972937   GH973106   GH974018   GH976161   GH977842   GH978999   GH979054   GH982250   GH983786
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V5E1 B7YZR2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0033466    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0033466 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0033466 expression level Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4778) embryo 02-04hr  (7247) embryo 04-06hr  (8344) embryo 06-08hr  (7072) embryo 08-10hr  (6450) embryo 10-12hr  (5604) embryo 12-14hr  (3866) embryo 14-16hr  (3651) embryo 16-18hr  (2605) embryo 18-20hr  (1680) embryo 20-22hr  (2721) embryo 22-24hr  (2317) larva L1  (1031) larva L2  (1205) larva L3 12hr old  (1578) larva L3 puffstage 1-2  (2664) larva L3 puffstage 3-6  (2988) larva L3 puffstage 7-9  (3080) white prepupae new  (3379) white prepupae 12hr  (2728) white prepupae 24hr  (3338) pupae 2d postWPP  (2853) pupae 3d postWPP  (1537) pupae 4d postWPP  (1360) adult male 01day  (2109) adult male 05day  (2509) adult male 30day  (2189) adult female 01day  (1837) adult female 05day  (2602) adult female 30day  (2818) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4778) embryo 02-04hr  (7247) embryo 04-06hr  (8344) embryo 06-08hr  (7072) embryo 08-10hr  (6450) embryo 10-12hr  (5604) embryo 12-14hr  (3866) embryo 14-16hr  (3651) embryo 16-18hr  (2605) embryo 18-20hr  (1680) embryo 20-22hr  (2721) embryo 22-24hr  (2317) larva L1    1031 larva L2    1205 larva L3 12hr old  (1578) larva L3 puffstage 1-2  (2664) larva L3 puffstage 3-6  (2988) larva L3 puffstage 7-9  (3080) white prepupae new  (3379) white prepupae 12hr  (2728) white prepupae 24hr  (3338) pupae 2d postWPP  (2853) pupae 3d postWPP  (1537) pupae 4d postWPP    1360 adult male 01day  (2109) adult male 05day  (2509) adult male 30day  (2189) adult female 01day  (1837) adult female 05day  (2602) adult female 30day  (2818) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0033466 expression level Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4778) embryo 02-04hr  (7247) embryo 04-06hr  (8344) embryo 06-08hr  (7072) embryo 08-10hr  (6450) embryo 10-12hr  (5604) embryo 12-14hr  (3866) embryo 14-16hr  (3651) embryo 16-18hr  (2605) embryo 18-20hr  (1680) embryo 20-22hr  (2721) embryo 22-24hr  (2317) larva L1  (1031) larva L2  (1205) larva L3 12hr old  (1578) larva L3 puffstage 1-2  (2664) larva L3 puffstage 3-6  (2988) larva L3 puffstage 7-9  (3080) white prepupae new  (3379) white prepupae 12hr  (2728) white prepupae 24hr  (3338) pupae 2d postWPP  (2853) pupae 3d postWPP  (1537) pupae 4d postWPP  (1360) adult male 01day  (2109) adult male 05day  (2509) adult male 30day  (2189) adult female 01day  (1837) adult female 05day  (2602) adult female 30day  (2818) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4778) embryo 02-04hr  (7247) embryo 04-06hr  (8344) embryo 06-08hr  (7072) embryo 08-10hr  (6450) embryo 10-12hr  (5604) embryo 12-14hr  (3866) embryo 14-16hr  (3651) embryo 16-18hr  (2605) embryo 18-20hr  1680 embryo 20-22hr  (2721) embryo 22-24hr  2317 larva L1    1031 larva L2    1205 larva L3 12hr old  1578 larva L3 puffstage 1-2  (2664) larva L3 puffstage 3-6  (2988) larva L3 puffstage 7-9  (3080) white prepupae new  (3379) white prepupae 12hr  (2728) white prepupae 24hr  (3338) pupae 2d postWPP  (2853) pupae 3d postWPP  1537 pupae 4d postWPP    1360 adult male 01day  2109 adult male 05day  (2509) adult male 30day  2189 adult female 01day  1837 adult female 05day  (2602) adult female 30day  (2818) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4778 embryo 02-04hr    7247 embryo 04-06hr    8344 embryo 06-08hr    7072 embryo 08-10hr    6450 embryo 10-12hr    5604 embryo 12-14hr    3866 embryo 14-16hr    3651 embryo 16-18hr    2605 embryo 18-20hr    1680 embryo 20-22hr    2721 embryo 22-24hr    2317 larva L1    1031 larva L2    1205 larva L3 12hr old    1578 larva L3 puffstage 1-2    2664 larva L3 puffstage 3-6    2988 larva L3 puffstage 7-9    3080 white prepupae new    3379 white prepupae 12hr    2728 white prepupae 24hr    3338 pupae 2d postWPP    2853 pupae 3d postWPP    1537 pupae 4d postWPP    1360 adult male 01day    2109 adult male 05day    2509 adult male 30day    2189 adult female 01day    1837 adult female 05day    2602 adult female 30day    2818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0033466    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0033466 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0033466 expression level Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0033466 expression level Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    47.175 Larval Midgut    41.8 Larval Hindgut    23.7 Larval Malpighian Tubules    31.2 Larval Fat Body    47.8 Larval Salivary Gland    42.3 Larval Trachea    22.175 Larval Carcass    13.45 Adult Head    38.2 Adult Eye    42.975 Adult Brain    39.4 Adult Thoracic-Abdominal Ganglion    50.9 Adult Crop    21.5 Adult Midgut    23.5 Adult Hindgut    25.1 Adult Malpighian Tubules    44.4 Adult Fat Body    77.9 Adult Salivary Gland    52.5 Adult Heart    57.6 Adult VirginFemale Spermatheca    71.8 Adult InseminatedFemale Spermatheca    82.7 Adult Ovary    44.2 Adult Testis    21.3 Adult Male Accessory Gland    118.3 Adult Carcass    47.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    38.2   NA  Eye    42.975   NA  Brain    39.4   47.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    50.9   NA  Crop    21.5   41.8  Midgut    23.5   23.7  Hindgut    25.1   31.2  Malpighian Tubules    44.4   47.8  Fat Body    77.9   42.3  Salivary Gland    52.5   NA  Heart    57.6   22.175  Trachea    NA   NA  VirginFemale Spermatheca    71.8   NA  InseminatedFemale Spermatheca    82.7   NA  Ovary    44.2   NA  Testis    21.3   NA  Male Accessory Gland    118.3   13.45  Carcass    47.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033466 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12130-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Pal1 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Pal1GD1907 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Pal1 Class Mutagen Stocks Known lesion Pal1NP1123 1 -- Pal101D09Y-M073 0 -- Pal15-HA-2753 0 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pal1 Class Mutagen Stocks Known lesion Pal1GD1907 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Pal1KK101622 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Partially disrupted in	Df(2R)228 (Han et al., 2004) Df(2R)307 (Han et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system P{GawB}Pal1NP1123 No miscellaneous insertions P{3'wHy}Pal101D09Y-M073 NA P{RS5}Pal15-HA-2753 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
peptidylamidoglycolate lyase activity inferred from direct assay (assigned by UniProtKB) (Han et al., 2004)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
neuronal cell body inferred from direct assay (assigned by UniProtKB) (Han et al., 2004)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
copper ion binding inferred from electronic annotation with InterPro:IPR000720 (FlyBase Curators et al., 2004-) peptidylglycine monooxygenase activity inferred from sequence or structural similarity with mouse Pam (FlyBase, 1992-)
Biological Process
CV term Evidence References
oxidation-reduction process inferred from electronic annotation with InterPro:IPR000720 (FlyBase Curators et al., 2004-) peptide metabolic process inferred from electronic annotation with InterPro:IPR000720 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR000720 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pal1 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0033466 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36033
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13565 Dere\GG24122 Dgri\GH21446 Dmoj\GI20251 Dper\GL16764 Dpse\GA11425 Dsec\GM21169 Dsim\GD10702 Dvir\GJ20204 Dwil\GK15744 Dyak\GE19318
Curated drosophilid orthologs	Dere\GG24122   Dpse\GA11425
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001565-PA Aedes aegypti (mosquito) AGAP007606-PA Anopheles gambiae (mosquito) XP_397510.3 Apis mellifera (honey bee) XP_001946502.1 Acyrthosiphon pisum (pea aphid) 127740 Branchiostoma floridae (lancelet) CPIJ000256 Culex pipens (mosquito) ENSCPOP00000010718 Cavia porcellus (guinea pig) 228792 Capitella spl (polychaete worm) ENSECAP00000006897 Equus caballus (horse) 128636 Lottia gigantea (owl limpet) ENSMUSP00000055295 Mus musculus (house mouse) XP_001606565.1 Nasonia vitripennis (wasp) ENSOANP00000008666 Ornithorynchus anatinus (platypus) PHUM009111-PA Pediculus humanus (human body louse) ENSRNOP00000014562 Rattus norvegicus (norway rat) XP_792034.2 Strongylocentrotus purpuratus (purple sea urchin) 63754 Trichoplax adhaerens (metazoa) ENSXETP00000051434 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13652 LH14730 FBgn0236668 FBgn0217747 FBgn0207344 FBgn0182467 FBgn0176050 JGI_V11_330720 FBgn0064444 FBgn0154368 FBgn0142987 FBgn0128908 FBgn0064653 FBgn0090594 CPIJ000256 BGIBMGA005815 ACYPI007331 GB17423 AGAP007606 AAEL001565 PB21880 PHUM387350
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Kyoto	103892 y* w*; P{GawB}Pal1NP1123 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v103604 P{KK101622}VIE-260B v7388 w1118; P{GD1907}v7388/CyO v7389 w1118; P{GD1907}v7389/CyO
Genomic Clones ( 2 )
	BACR01L01 BACR06J01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 99 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD34757
Other clones	FI05303
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04137 AT13308 AT13376 AT20791 AT21568 AT28128 AT31644 GH05989 LD04291 LD06771 LD15681 LD16565 LD31768 LD32878 LD33036 LD35193 LD36928 LD38508 LD47674 LP02283 LP02402 LP07025 RE03305 RE16478 RE17618 RE17619 RE18995 RE35689 RE42824 RE44145 RE49380 RE51280 RE53238 RE53338 RE54075 RE71781 RH03392 RH04121 RH04239 RH11627 RH13279 RH13290 RH52013 SD13282 SD15128 SD23243
Other clones	30808 67106 101278 106798 147719 172678 191198 194276 CK01.17C.B6 CK01.94A.F11 Dmel_neb_015968_0322_2274 EK026209 EK073322 EK111301 EK140335 EK195049 EK244346 ESG015d.E21.1_F01 RH01357 RP004115205 RP004133521 RP004155603 RP004185467 RP004205344 RP004215223 RP004222561 RP004222823 RP004225208 RP004227876 RP004238381 RP004247862 RP004249740 RP004253628 RP004255580 RP004257953 RP004258341 RP004258858 RP004276583 RP004325774 RP004371927 RP004387335 RP004389383 RP004403824 RP004404082 RP004435117 RP004452006 RP004458886 RP004460635 RP004500389 RP004550512 SD18319
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033466 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36033
Antibody Information
	polyclonal (Han et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Pal1 CG12130 (Han et al., 2004)
Source for database merge of	Source for merge of: CG12130 anon-WO0149856.3 (FlyBase, 1996-)
Additional comments	Source for merge of CG12130 anon-WO0149856.3 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136693 NM_001144158 RefSeq (Proteins) • NP_610537 NP_001137630 DDBJ / EMBL / GenBank • AA990839 AAF58870 AAK93266 ACL83084 AW943245 AX191631 AY051842 Entrez Gene - A searchable database of RefSeq genes. • 36033 UniProtKB/TrEMBL • B7YZR2 Q9V5E1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidyl-glycine alpha-amidating monooxygenase (IPR000720) NHL repeat, subgroup (IPR013017) Six-bladed beta-propeller, TolB-like (IPR011042) NHL repeat (IPR001258)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0033466 DRSC - Results from RNAi screens. • FBgn0033466 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033466 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12130-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033466 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36033 InParanoid A subset of ortholog calls from InParanoid. • AAEL001565-PA Aedes aegypti (mosquito) AGAP007606-PA Anopheles gambiae (mosquito) XP_397510.3 Apis mellifera (honey bee) XP_001946502.1 Acyrthosiphon pisum (pea aphid) 127740 Branchiostoma floridae (lancelet) CPIJ000256 Culex pipens (mosquito) ENSCPOP00000010718 Cavia porcellus (guinea pig) 228792 Capitella spl (polychaete worm) ENSECAP00000006897 Equus caballus (horse) 128636 Lottia gigantea (owl limpet) ENSMUSP00000055295 Mus musculus (house mouse) XP_001606565.1 Nasonia vitripennis (wasp) ENSOANP00000008666 Ornithorynchus anatinus (platypus) PHUM009111-PA Pediculus humanus (human body louse) ENSRNOP00000014562 Rattus norvegicus (norway rat) XP_792034.2 Strongylocentrotus purpuratus (purple sea urchin) 63754 Trichoplax adhaerens (metazoa) ENSXETP00000051434 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36033 modMine - Data generated by the modENCODE project. • FBgn0033466 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13652 LH14730 FBgn0236668 FBgn0217747 FBgn0207344 FBgn0182467 FBgn0176050 JGI_V11_330720 FBgn0064444 FBgn0154368 FBgn0142987 FBgn0128908 FBgn0064653 FBgn0090594 CPIJ000256 BGIBMGA005815 ACYPI007331 GB17423 AGAP007606 AAEL001565 PB21880 PHUM387350
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	anon-WO0149856.3   CG12130 (Han et al., 2004, Molnar et al., 2006, Keleman et al., 2009.8.5) dPAL1 (Han et al., 2004) DPAL1 (Guenin et al., 2010) Pal1   PAL1 (Han et al., 2004)
Name Synonym	Peptidyl-α-hydroxyglycine-α-amidating lyase 1
Secondary FlyBase IDs
	FBgn0045949
References ( 14 )
Research paper	Guenin et al., 2010, BMC Genomics 11: 47 Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth. [FBrf0210012] Molnar et al., 2006, Genetics 174(3): 1635--1659 A gain-of-function screen identifying genes required for vein formation in the Drosophila melanogaster wing. [FBrf0195387] Han et al., 2004, J. Neurochem. 90(1): 129--141 Drosophila uses two distinct neuropeptide amidating enzymes, dPAL1 and dPAL2. [FBrf0179240]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Patent	Stout et al., 2001.7.12, World Patent 0149856-A: Drosophila enzymes, encoding nucleic acids and methods of use. [FBrf0141239]
Curated genome annotation	Zhu, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126704]