FlyBase Gene Report: Dmel\CAP

Comment: in longitudinal muscle

(Bharadwaj et al., 2013)

longitudinal muscle

(Bharadwaj et al., 2013)

third instar larval stage

Comment: in longitudinal muscle

(Bharadwaj et al., 2013)

scolopidial dendritic cap cell

(Bharadwaj et al., 2013)

(Bharadwaj et al., 2013)

day 7 of adulthood -- day 49 of adulthood

(Bharadwaj et al., 2013)

lch5 cap attachment cell

(Bharadwaj et al., 2013)

adult stage

Johnston organ

(Bharadwaj et al., 2013)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

CAP protein is found in stage 16 embryos predominantly in chordotonal organs, somatic muscle, dorsal vessel and the gut. It is present in scolopale cells and muscle attachment sites of longitudinal muscles but is missing from lateral transverse muscles. In third instar larvae it is found mainly in scolopale cells and weakly in cap cells and is also at muscle attachment sites. Isoform-specific expression of CAP protein is observed.

(Bharadwaj et al., 2013)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{GT1}CAP^BG02184

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

olfactory receptor neuron | subset

(Tunstall et al., 2012)

eo support cell of olfactory sensory organ | subset

(Tunstall et al., 2012)

Reporter: P{PTT-GA}CAP^CA06924

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

(Bharadwaj et al., 2013)

third instar larval stage

(Bharadwaj et al., 2013)

(Bharadwaj et al., 2013)

View Dmel\CAP in GBrowse 2

(Bharadwaj et al., 2013)

lch5 cap attachment cell

(Bharadwaj et al., 2013)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CAP

View exonic expression by tissue for Dmel\CAP

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033504

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0033504

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG18408-RI

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18408

Flygut - An atlas of the Drosophila adult midgut

FBgn0033504

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CAP

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CAP

Transgenic constructs containing regulatory region of CAP

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal (with Df(2R)BSC281)

CAP^{MI02963-TG4.0}

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CAP^GD8545

viable, with Scer\GAL4^Mef2.PR

CAP^GD8545

viable, with Scer\GAL4^pnr-MD237

CAP^GD8545

viable, with Scer\GAL4^tin.CΔ4

CAP^GD8545

Sterility

Allele

fertile

Other Phenotypes

Allele

auditory perception defective | adult stage

CAP^49e

auditory perception defective | adult stage, with Scer\GAL4^nompA.cK

CAP^miRNA.UAS

behavior defective | third instar larval stage

behavior defective | third instar larval stage, with Scer\GAL4^nompA.cK

CAP^miRNA.UAS

neuroanatomy defective | adult stage

CAP^49e

Phenotype manifest in

Allele

Johnston organ

CAP^49e

muscle attachment site, with Scer\GAL4^Mef2.PR

CAP^{E.UAS.Tag:MYC}

muscle attachment site | larval stage

muscle attachment site | larval stage, with Scer\GAL4^Mef2.PR

CAP^miRNA.UAS

Download All DIOPT Orthologs

CAP^49e

Orthologs

Downloads

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SORBS3

7 of 15

Yes

Hsap\SORBS1

5 of 15

Yes

Hsap\SORBS2

5 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\NOXA1

1 of 15

Yes

Hsap\SH3D19

1 of 15

Hsap\SH3RF1

1 of 15

Hsap\SH3RF2

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Sorbs3

7 of 15

Yes

Mmus\Sorbs1

5 of 15

Yes

Mmus\Sorbs2

5 of 15

Yes

Mmus\Dnmbp

1 of 15

Mmus\Ncf2

1 of 15

Yes

Mmus\Noxa1

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (8)

Rnor\Sorbs3

4 of 13

Yes

Rnor\Sorbs2

3 of 13

Yes

Rnor\Dnmbp

1 of 13

Yes

Rnor\Ncf2

1 of 13

Yes

Rnor\Noxa1

1 of 13

Yes

Rnor\Sh3rf1

1 of 13

Rnor\Sh3rf2

1 of 13

Rnor\Sh3rf3

1 of 13

Xenopus tropicalis (Western clawed frog) (8)

Xtro\sorbs1

3 of 12

Yes

Xtro\sorbs2

3 of 12

Yes

Xtro\ankrd12

1 of 12

Xtro\dnmbp

1 of 12

Yes

Xtro\ncf2

1 of 12

Yes

Xtro\noxa1

1 of 12

Yes

Xtro\sh3d19

1 of 12

Xtro\sorbs3

1 of 12

Yes

Danio rerio (Zebrafish) (10)

Drer\sorbs3

4 of 15

Yes

Drer\sorbs2a

3 of 15

Yes

Drer\sorbs2b

3 of 15

Yes

Drer\sorbs1

2 of 15

Yes

Drer\dnmbp

1 of 15

Drer\ncf2

1 of 15

Yes

Drer\noxa1

1 of 15

Yes

Drer\sh3d19

1 of 15

Drer\sh3rf1

1 of 15

Drer\sh3rf2

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\sorb-1

5 of 15

Yes

Cele\B0303.7

1 of 15

Yes

Cele\C36E8.4

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\AT1G31440

1 of 9

Yes

Atha\AT4G18060

1 of 9

Yes

Atha\AT4G34660

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\BEM1

1 of 15

Yes

Scer\LSB3

1 of 15

Yes

Scer\RVS167

1 of 15

Yes

Scer\YSC84

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\sfp47

1 of 12

Yes

Spom\SPBC119.05c

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190040 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA24375

Drosophila persimilis

Dper\GL10457

Drosophila willistoni

Dwil\GK17791

Drosophila virilis

Dvir\GJ22205

Drosophila mojavensis

Dmoj\GI19326

Drosophila grimshawi

Dgri\GH21781

Drosophila grimshawi

Dgri\GH21778

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915002N )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17024037

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03841

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

CAP^BG02184

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

CAP^KG00308

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SORBS3; sorbin and SH3 domain containing 3

7 of 15

610795

SORBS1; sorbin and SH3 domain containing 1

5 of 15

605264

SORBS2; sorbin and SH3 domain containing 2

5 of 15

616349

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CAP

pull down, western blot

(Yamazaki and Nusse, 2002)

CAP - Axn

pull down, western blot, two hybrid

(Yamazaki and Yanagawa, 2003, Yamazaki and Nusse, 2002)

CAP - CG13144

(Hu et al., 2017.6.13)

CAP - CG2931

(Hu et al., 2017.6.13)

CAP - CG31244

(Hu et al., 2017.6.13)

CAP - CG32440

(Hu et al., 2017.6.13)

CAP - CG44251

(Hu et al., 2017.6.13)

CAP - CG5011

(Hu et al., 2017.6.13)

CAP - CG5111

(Hu et al., 2017.6.13)

CAP - CG9667

(Hu et al., 2017.6.13)

CAP - CycK

(Hu et al., 2017.6.13)

CAP - Gem2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Hu et al., 2017.6.13)

CAP - HIPP1

(Alekseyenko et al., 2014)

CAP - LUBEL

(Hu et al., 2017.6.13)

CAP - Lasp

(Hu et al., 2017.6.13)

CAP - PolZ2

(Hu et al., 2017.6.13)

CAP - Pu

(Hu et al., 2017.6.13)

CAP - RnpS1

(Hu et al., 2017.6.13)

CAP - SmydA-8

anti tag coimmunoprecipitation, peptide massfingerprinting

(Hu et al., 2017.6.13)

CAP - Su(var)205

(Alekseyenko et al., 2014)

CAP - Tektin-A

(Hu et al., 2017.6.13)

CAP - Trs33

(Hu et al., 2017.6.13)

CAP - VhaAC39-1

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, peptide massfingerprinting

(Hu et al., 2017.6.13)

CAP - Vinc

(Hu et al., 2017.6.13, Bharadwaj et al., 2013)

CAP - Vps28

(Hu et al., 2017.6.13)

CAP - Vsx2

(Hu et al., 2017.6.13)

CAP - Vta1

(Hu et al., 2017.6.13)

CAP - arr

pull down, western blot

(Yamazaki and Yanagawa, 2003)

CAP - bowl

(Hu et al., 2017.6.13)

CAP - dsh

(Yamazaki and Yanagawa, 2003)

CAP - robl22E

(Hu et al., 2017.6.13)

CAP - sol