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Gene Dmel\CG12895

General Information
Symbol	Dmel\CG12895	Species	D. melanogaster
Name		Annotation symbol	CG12895
Feature type	protein_coding_gene	FlyBase ID	FBgn0033523
Gene Model Status	Current	Stock availability	6 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	47A7-47A7	Sequence location	2R:6,324,103..6,324,887 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG12895 (FBgn0033523). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 5 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein of unknown function DUF339. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1632971_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG12895. Gene sequence location is 2R:6324103..6324887.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47A7-47A7   Limits computationally determined from genome sequence between P{lacW}Hr46k10308 and P{PZ}lola00349&P{EP}lolaEP952
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG12895 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG12895-RA FBtr0088314  NM_136742   593   156 CG12895-RB FBtr0330615  NM_001273939   667   156
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG12895-PA   FBpp0087405   18.4   156   6.12     NP_610586   AAF58794 CG12895-PB   FBpp0303465   18.4   156   6.12     NP_001260868   AGB93401
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein of unknown function DUF339 (IPR005631)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58794   AE013599 AGB93401   AY071255 AAL48877   BI233158   BI235331   BI236636   BQ102928   BT132881   CO196108   CO262173   CO263221   CO263465   CO263659   CO265990   CO270258   CO270581   CO285868   CO286449   CO288871   CO288877   CO289058   CO290895   CO291101   CO291239   CO301265   CO305957   CO318434   CO341791   CO341881   EC058545   EC058866   EC058963   EC059079   EC071003   EC074218   EC082431   EC195983   EC196268   EC196271   EC196636   EC196676   EC204331   EC207368   EC208272   EC208273   EC219603   EC244827   EC255256   EC259798   EV592697   FD585529   FD585534   FD587215   FD587277   GH739398   GH739653   GH741272   GH741515   GH741864   GH742530   GH743376   GH745601   GH746457   GH747288   GH747420   GH747533   GH761969   GH763370   GH766053   GH766554   GH768063   GH768453   GH779278   GH779639
UniProtKB/Swiss-Prot	A1Z897
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    212.7 Larval Midgut    102.8 Larval Hindgut    167.9 Larval Malpighian Tubules    89.7 Larval Fat Body    59.2 Larval Salivary Gland    133.6 Larval Trachea    216.625 Larval Carcass    153.725 Adult Head    195.4 Adult Eye    227.425 Adult Brain    230.5 Adult Thoracic-Abdominal Ganglion    186.9 Adult Crop    212.1 Adult Midgut    168.8 Adult Hindgut    314.6 Adult Malpighian Tubules    130.6 Adult Fat Body    115.3 Adult Salivary Gland    183.5 Adult Heart    193.05 Adult VirginFemale Spermatheca    74.9 Adult InseminatedFemale Spermatheca    86.5 Adult Ovary    178.4 Adult Testis    85.7 Adult Male Accessory Gland    144 Adult Carcass    187.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    195.4   NA  Eye    227.425   NA  Brain    230.5   212.7  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    186.9   NA  Crop    212.1   102.8  Midgut    168.8   167.9  Hindgut    314.6   89.7  Malpighian Tubules    130.6   59.2  Fat Body    115.3   133.6  Salivary Gland    183.5   NA  Heart    193.05   216.625  Trachea    NA   NA  VirginFemale Spermatheca    74.9   NA  InseminatedFemale Spermatheca    86.5   NA  Ovary    178.4   NA  Testis    85.7   NA  Male Accessory Gland    144   153.725  Carcass    187.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    17 central nervous system, pupae P8    13 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    9 head, mated 4-day female    14 head, mated 20-day female    9 head, mated 1-day male    13 head, mated 4-day male    20 head, mated 20-day male    25 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    13 fat body, larvae L3 wandering    3 fat body, white prepupae    7 fat body, pupae P8    16 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    24 ovary, mated 4-day female    25 testis, mated 4-day male    16 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr  (37) embryo 04-06hr  (30) embryo 06-08hr    23 embryo 08-10hr  (27) embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr  (37) embryo 04-06hr  30 embryo 06-08hr    23 embryo 08-10hr  27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    37 embryo 04-06hr    30 embryo 06-08hr    23 embryo 08-10hr    27 embryo 10-12hr    22 embryo 12-14hr    24 embryo 14-16hr    15 embryo 16-18hr    22 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    25 larva L1    18 larva L2    24 larva L3 12hr old    19 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    15 white prepupae 24hr    16 pupae 2d postWPP    20 pupae 3d postWPP    21 pupae 4d postWPP    10 adult male 01day    18 adult male 05day    21 adult male 30day    17 adult female 01day    15 adult female 05day    23 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (40) Schneider line 2 Sg4  (31) embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167  (38) embryonic S1  (29) embryonic S3  (40) leg disc CME L1  (32) wing disc CME-W2  (57) wing disc ML-DmD8  (28) wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  (31) CNS ML-DmBG2-c2  (50) tumorous blood cells mbn2  (28) ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (40) Schneider line 2 Sg4  31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167  (38) embryonic S1  29 embryonic S3  (40) leg disc CME L1  32 wing disc CME-W2  (57) wing disc ML-DmD8  28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  31 CNS ML-DmBG2-c2  (50) tumorous blood cells mbn2  28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    31 embryonic 1182-4H    25 embryonic GM2    22 embryonic Kc167    38 embryonic S1    29 embryonic S3    40 leg disc CME L1    32 wing disc CME-W2    57 wing disc ML-DmD8    28 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    18 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    50 tumorous blood cells mbn2    28 ovary fGS/OSS    9 ovary OSC    25 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3  26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3  26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    19 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_19 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • RE29446 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033523 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12895-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG12895GD7591 viable, with Scer\GAL4pnr-MD237 CG12895GD17338 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 CG12895GD7591 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 CG12895GD7591 trichogen cell, with Scer\GAL4pnr-MD237 CG12895GD7591
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG12895 Class Mutagen Stocks Known lesion CG12895G19671 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG12895 Class Mutagen Stocks Known lesion CG12895GD17338 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG12895GD7591 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG12895KK110799 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG12895NIG.12895R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7591} NA P{GD17338} NA P{KK110799} NA P{NIG.12895R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}CG12895G19671 NA P{EPgy2}GαoEY04711 NA
Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG12895 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61921 DroID - A comprehensive database of gene and protein interactions. • FBgn0033523 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36103
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VMG1W Dipteran inclusive ortholog search EOG644KVM Insect inclusive ortholog search EOG6CFZ5R Arthropod inclusive ortholog search EOG6KSN27 Metazoa inclusive ortholog search EOG661H00
Orthologs in Drosophila Species (EOG6VMG1W)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG12895     Drosophila simulans   Dsim\GD25982 Y   Drosophila sechellia   Dsec\GM20523 Y   Drosophila erecta   Dere\GG25208 Y   Drosophila yakuba   Dyak\GE21507 Y   Drosophila ananassae   Dana\GF11110 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA25039 Y   Drosophila persimilis   Dper\GL17204 Y   Drosophila willistoni   Dwil\GK18008 Y   Drosophila virilis   Dvir\GJ20144 Y   Drosophila mojavensis   Dmoj\GI20197 Y   Drosophila grimshawi   Dgri\GH20634 Y
Orthologs in non-Drosophila Dipterans (EOG644KVM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005866 Y Anopheles gambiae Malaria mosquito  Agam\AGAP012191 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ019830 Y
Orthologs in non-Dipteran Insects (EOG6CFZ5R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48083 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013992 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003347 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14618 Y Atta cephalotes Leafcutter ant  Acep\ACEP11927 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO15019 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14878 Y Linepithema humile Argentine ant  Lhum\LH13822 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB11176 Y Solenopsis invicta Red fire ant  Sinv\SINV12246 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004221 Y Bombyx mori Silkmoth  Bmor\BGIBMGA001236 Y Pediculus humanus Human body louse  Phum\PHUM261820 Y Tribolium castaneum Red flour beetle  Tcas\TC001151 Y
Orthologs in non-Insect Arthropods (EOG6KSN27)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\RPB2 Y Ixodes scapularis Deer tick  Isca\ISCW001947 Y
Orthologs in non-Arthropod Metazoa (EOG661H00)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y57A10A.29 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015703gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024606gn Y Danio rerio Zebrafish  Drer\sdhaf2 Y Xenopus tropicalis Western clawed frog  Xtro\sdhaf2 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000024418 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000013301 Y Mus musculus House mouse  Mmus\Sdhaf2 Y Rattus norvegicus Norway rat  Rnor\Sdhaf2 Y Homo sapiens Human  Hsap\ENSG00000256591 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000256591
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25982 Drosophila sechellia Dsec\GM20523 Drosophila erecta Dere\GG25208 Drosophila yakuba Dyak\GE21507 Drosophila ananassae Dana\GF11110 Drosophila pseudoobscura pseudoobscura Dpse\GA25039 Drosophila persimilis Dper\GL17204 Drosophila willistoni Dwil\GK18008 Drosophila virilis Dvir\GJ20144 Drosophila mojavensis Dmoj\GI20197 Drosophila grimshawi Dgri\GH20634
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	28114 y1 w*; P{EP}CG12895G19671
VDRC	v102117 P{KK110799}VIE-260B v18093 w1118; P{GD7591}v18093 v18095 w1118; P{GD7591}v18095 v49606 w1118; P{GD17338}v49606 v49607 w1118; P{GD17338}v49607
Genomic Clones ( 1 )
	BACR20O22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 69 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE29446
Other clones	FI17302
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE31162 RE32715
Other clones	34838 58195 167088 214317.36 214409.49 CK01.77C.H4 CK01.78B.A10 CK01.81B.C6 CK01.88B.F6 CK01.88B.H6 CK01.89A.C5 CK01.94B.C11 CK01.97C.D1 CK01.98C.B4 CK01.99D.G4 CK01.105A.E9 CK01.106D.G10 CK01.110A.E4 Dmel_neb_011946_0689_0452 EK001007 EK061033 EK062843 EK069555 EK069563 EK070011 EK075513 EK076129 EK076858 EK084558 EK087819 EK088608 EK089159 EK098106 EK099350 EK144632 EK180611 EK249902 EK272129 EP13147 EP13334 RM02685 RM02885 RM03185 RM03385 RP001010581 RP001014934 RP001014953 RP001014983 RP001017797 RP001017893 RP001019626 RP001022251 RP001025206 RP001026578 RP001026644 RP001035456 RP001038451 RP001041516 RP001041624 RP001045300 RP001046387 RP001048658 RP001051052 RP001052102 UUGC0322
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033523 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36103
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG12895 BcDNA:RE29446 (FlyBase, 1996-)
Additional comments	Source for merge of CG12895 BcDNA:RE29446 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136742 NM_001273939 RefSeq (Proteins) • NP_610586 NP_001260868 DDBJ / EMBL / GenBank • AAF58794 AAL48877 AGB93401 AY071255 BI233158 Entrez Gene - A searchable database of RefSeq genes. • 36103 UniProtKB/Swiss-Prot • A1Z897
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein of unknown function DUF339 (IPR005631) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE29446
Linkouts
	BioGRID - A database of protein and genetic interactions • 61921 DroID - A comprehensive database of gene and protein interactions. • FBgn0033523 DRSC - Results from RNAi screens. • FBgn0033523 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033523 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12895-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033523 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36103 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36103 modMine - Data generated by the modENCODE project. • FBgn0033523
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	BcDNA:RE29446   CG12895 (Keleman et al., 2009.8.5, Strub et al., 2008, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym
Secondary FlyBase IDs
	FBgn0047132
References ( 13 )
Research paper	Strub et al., 2008, Genome 51(6): 409--420 Mutations of the withered (whd) gene in Drosophila melanogaster confer hypersensitivity to oxidative stress and are lesions of the carnitine palmitoyltransferase I (CPT I) gene. [FBrf0204832]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Bellen, 2009.6.29, BDGP_2009_0629.GenExel.xml BDGP_2009_0629.GenExel.xml [FBrf0208121] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]