FB2021_01, released Feb 18, 2021

Gene: Dmel\CG12895

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General Information
Symbol
Dmel\CG12895
Species
D. melanogaster
Name
Annotation Symbol
CG12895
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033523
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Key Links
Genomic Location
Cytogenetic map
47A7-47A7
Sequence location
2R:10,436,598..10,437,382 [-]
Recombination map

2-62

RefSeq locus
NT_033778 REGION:10436598..10437382
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (0 terms)
There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2014-09-09 )
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in mitochondrial electron transport, succinate to ubiquinone
inferred from sequence or structural similarity with UniProtKB:Q9NX18
(assigned by UniProt )
(UniProt Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000279729
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitochondrial respiratory chain complex II assembly
inferred from biological aspect of ancestor with PANTHER:PTN000279729
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein-FAD linkage
inferred from biological aspect of ancestor with PANTHER:PTN000279729
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q9NX18
(assigned by UniProt )
(UniProt Curators, 2002-)
involved_in tricarboxylic acid cycle
inferred from biological aspect of ancestor with PANTHER:PTN000279729
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000279729
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q9NX18
(assigned by UniProt )
(UniProt Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the SDHAF2 family. (A1Z897)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.
(UniProt, A1Z897)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\CG12895 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
CG12895-RA
FBtr0088314
NM_136742
593
156
CG12895-RB
FBtr0330615
NM_001273939
667
156
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
CG12895-PA
FBpp0087405
18.4
156
6.12
NP_610586
AAF58794
CG12895-PB
FBpp0303465
18.4
156
6.12
NP_001260868
AGB93401
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

156 aa isoforms: CG12895-PA, CG12895-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with the flavoprotein subunit within the SDH catalytic dimer.

(UniProt, A1Z897)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG12895 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic/larval midgut
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG12895 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CG12895
Transgenic constructs containing regulatory region of CG12895
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation
Inferred from location
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SDHAF2
15 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Sdhaf2
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Sdhaf2
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\sdhaf2
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\sdhaf2
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\Y57A10A.29
10 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\SDHAF2
4 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SDH5
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC12B10.06c
9 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190IJ6 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002821
Drosophila simulans
Dsim\GD25982
Drosophila sechellia
Dsec\GM20523
Drosophila erecta
Dere\GG25208
Drosophila yakuba
Dyak\GE21507
Drosophila ananassae
Dana\GF11110
Drosophila pseudoobscura pseudoobscura
Dpse\GA25039
Drosophila persimilis
Dper\GL17204
Drosophila willistoni
Dwil\GK18008
Drosophila virilis
Dvir\GJ20144
Drosophila mojavensis
Dmoj\GI20197
Drosophila grimshawi
Dgri\GH20634
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150GFG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17016033
Glossina morsitans
Tsetse fly
Gmor\GMOY008220
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11307
Mayetiola destructor
Hessian fly
Mdes\Mdes004154
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005866
Anopheles darlingi
American malaria mosquito
Adar\ADAC001998
Anopheles gambiae
Malaria mosquito
Agam\AGAP012191
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ019830
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0JJ1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA001236
Danaus plexippus
Monarch butterfly
Dple\DPOGS211864-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL004809
Apis florea
Little honeybee
Aflo\3204479508
Apis mellifera
Western honey bee
Amel\GB48083
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16742
Bombus terrestris
Buff-tailed bumblebee
Bter\15211882
Linepithema humile
Argentine ant
Lhum\LH13822
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001dc8
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003347
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG013992
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_11014
Tribolium castaneum
Red flour beetle
Tcas\TC001151
Pediculus humanus
Human body louse
Phum\PHUM261820
Rhodnius prolixus
Kissing bug
Rpro\RPRC001704
Cimex lectularius
Bed bug
Clec\CLEC001998
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004221
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10130
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0JNY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012039
Ixodes scapularis
Black-legged tick
Isca\ISCW001947
Stegodyphus mimosarum
African social velvet spider
Smim\KFM75570.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur01g15410
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_40617
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0VSZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_015703gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Flj20487l
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000014147
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000024418
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG14757
10 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    SDHAF2; succinate dehydrogenase complex assembly factor 2
    model of  paraganglioma
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SDHAF2; succinate dehydrogenase complex assembly factor 2
    15 of 15
    613019
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with the flavoprotein subunit within the SDH catalytic dimer.
    (UniProt, A1Z897 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-62

    Cytogenetic map
    47A7-47A7
    Sequence location
    2R:10,436,598..10,437,382 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    47A7-47A7
    Limits computationally determined from genome sequence between P{lacW}Hr46k10308 and P{PZ}lola00349&P{EP}lolaEP952
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-62

    (FlyBase, 2016)

    2-61.1

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (16)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (70)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: CG12895 BcDNA:RE29446

    (FlyBase, 1996-)
    Additional comments

    Source for merge of CG12895 BcDNA:RE29446 was a shared cDNA ( date:030728 ).

    (FlyBase, 1996-)
    Other Comments
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 31 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Synonyms and Secondary IDs (2)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (30)