Gene Dmel\MCPH1

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General Information

Symbol

Dmel\MCPH1

Species

D. melanogaster

Name

Microcephalin

Annotation symbol

CG8981

Feature type

protein_coding_gene

FlyBase ID

FBgn0033664

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

48C5-48C5

Sequence location

2R:7,784,347..7,791,419 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Microcephalin is referred to in FlyBase by the symbol MCPH1 (CG8981, FBgn0033664). It has the cytological map location 48C5. Its sequence location is 2R:7784347..7791419. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48C5-48C5

Limits computationally determined from genome sequence between P{lacW}Ef1α48D^k17014 and P{lacW}l(2)k06612^k06612

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\MCPH1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

MCPH1-RA

FBtr0088055

NM_165852

3037

779

MCPH1-RB

FBtr0088056

NM_136867

2836

826

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

MCPH1-PA

FBpp0087161

86.2

779

10.24

NP_725088

AAF58607

MCPH1-PB

FBpp0087162

91.0

826

10.42

NP_610711

AAF58605

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033664

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

BRCT (IPR001357)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

bs21a07

(Oliver, 2001.8.16)

LD43341

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12885

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI511813
AI389510

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033664

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033664

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033664

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG8981

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for MCPH1

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

MCPH1^EY11307

Sterility

Allele

fertile

MCPH1^EY11307

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of MCPH1	Class	Mutagen	Stocks	Known lesion
MCPH1^EY11307		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of MCPH1	Class	Mutagen	Stocks	Known lesion
MCPH1^GD12537		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful duplication

Dp(2;2)Y3b

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12537}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}MCPH1^EY11307

P{Mae-UAS.6.11}B1505^B1505

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR001357

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

MCPH1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62057

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11801

Dere\GG22621

Dmoj\GI19591

Dpse\GA24135

Dsec\GM20401

Dsim\GD25875

Dvir\GJ18380

Dyak\GE13490

Curated drosophilid orthologs

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017923
Anopheles gambiae (Ensembl)
ENSCINP00000003528
Ciona intestinalis (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v28098

w¹¹¹⁸; P{GD12537}v28098

v28100

w¹¹¹⁸; P{GD12537}v28100

Bloomington

20289

y¹ w^67c23; P{EPgy2}MCPH1^EY11307 CG13189^EY11307

Genomic Clones ( 1 )

BACR14L19

cDNA Clones ( 46 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033664

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033664

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: MCPH1 CG8981

(Ponting, 2005)

Source for database merge of

Source for merge of: CG13184 CG8981 Source for merge of CG13184 CG8981 was a shared cDNA (date:010720).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

BRCT (IPR001357)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG8981

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62057

DEDB - Drosophila exon database: splicing graphs

•

12885

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033664

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033664

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033664

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0033664

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0033664

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033664

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033664

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017923
Anopheles gambiae (Ensembl)
ENSCINP00000003528
Ciona intestinalis (Ensembl)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/genebrief/microcephalin.htm

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033664

University of Chicago Dev. Expression

•

FBgn0033664

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

CG8981

(Stathopoulos et al., 2004, Inlow and Restifo, 2004, Giot et al., 2003, Grieder et al., 2006)

CG13184

Dmcph1

(Brunk et al., 2007)

MCPH1

(Rickmyre et al., 2007, Garcia et al., 2007)

Name Synonym

Microcephalin

(Ponting, 2005)

Secondary FlyBase IDs

FBgn0033666

References ( 16 )

Research paper

Inlow and Restifo, 2004, Genetics 166(2): 835--881: Molecular and comparative genetics of mental retardation. [FBrf0174714]
Stathopoulos et al., 2004, Genes Dev. 18(6): 687--699: pyramus and thisbe: fGF genes that pattern the mesoderm of Drosophila embryos. [FBrf0174649]

Supplementary material

Grieder et al., 2006, Genetics 175(4):: Supplemental Data. [FBrf0204785]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Review

Ponting, 2005, Curr. Opin. Genet. Dev. 15(3): 241--248: [title not yet available] [FBrf0191564]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Oliver, 2001.8.16, File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348).: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]

Abstract

Brunk et al., 2007, Europ. Dros. Res. Conf. 20: F033: MICROCEPHALIN COORDINATES MITOSIS IN THE SYNCYTIAL EMBRYO. [FBrf0199835]
Rickmyre et al., 2007, A. Dros. Res. Conf. 48: 197B: Microcephalin (MCPH1) is required for cell-cycle progression in the early Drosophila embryo. [FBrf0198001]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Conference report

Garcia et al., 2007, Fly 1(2): 125--131: Cell cycle regulation. [FBrf0200076]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]