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Gene Dmel\Hen1

General Information
Symbol	Dmel\Hen1	Species	D. melanogaster
Name		Annotation symbol	CG12367
Feature type	protein_coding_gene	FlyBase ID	FBgn0033686
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	PIMET, DmHen1
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48E2-48E3	Sequence location	2R:8,033,215..8,040,257 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\Hen1 (CG12367, FBgn0033686). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: O-methyltransferase activity. There is experimental evidence that it is involved in the biological process: posttranscriptional gene silencing by RNA; RNA methylation. 12 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: 3'-RNA ribose 2'-O-methyltransferase, Hen1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1623887_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hen1. Gene sequence location is 2R:8033215..8040257.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48E2-48E3   Limits computationally determined from genome sequence between P{lacW}jebk05644 and P{PZ}SmD302833
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hen1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hen1-RA FBtr0087984  NM_136888   1573   391 Hen1-RB FBtr0333062  NM_001273983   1500   309
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hen1-PA   FBpp0087092   45.6   391   4.44     NP_610732   AAF58575 Hen1-PB   FBpp0305276   35.9   309   4.29     NP_001260912   AGB93445
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • 3'-RNA ribose 2'-O-methyltransferase, Hen1 (IPR026610)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201403   AE013599 AAF58575   AE013599 AGB93445   AW942054   AY069325 AAL39470   BI171194   BI639246   BP548039   CL844358   CO176789   CO188132   CO289018   CO289609   CO345294   CZ480807   CZ483293   EC070207   EC090554   EC196326   EC206692   EC254011   GH739762   GH742897   GH743729   GH743755   GH744453   GH744475   GH745772   GH745971   GH761656   GH762422   GH764232   GH768142   GH769005   GH769706   GH769742
UniProtKB/Swiss-Prot	Q7K175
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) RT-PCR Stage Tissue/Position (including subcellular localization) Reference adult stage | female   ovary   (Horwich et al., 2007) adult stage | male   testis   (Horwich et al., 2007)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    68.6 Larval Midgut    12.5 Larval Hindgut    61.1 Larval Malpighian Tubules    43.7 Larval Fat Body    52.3 Larval Salivary Gland    48.9 Larval Trachea    83.1 Larval Carcass    39 Adult Head    32.5 Adult Eye    30.125 Adult Brain    28.9 Adult Thoracic-Abdominal Ganglion    23.6 Adult Crop    52 Adult Midgut    20.7 Adult Hindgut    33 Adult Malpighian Tubules    45 Adult Fat Body    56.6 Adult Salivary Gland    44.6 Adult Heart    54.225 Adult VirginFemale Spermatheca    35.7 Adult InseminatedFemale Spermatheca    49.6 Adult Ovary    99.9 Adult Testis    177 Adult Male Accessory Gland    95.8 Adult Carcass    34.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    32.5   NA  Eye    30.125   NA  Brain    28.9   68.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    23.6   NA  Crop    52   12.5  Midgut    20.7   61.1  Hindgut    33   43.7  Malpighian Tubules    45   52.3  Fat Body    56.6   48.9  Salivary Gland    44.6   NA  Heart    54.225   83.1  Trachea    NA   NA  VirginFemale Spermatheca    35.7   NA  InseminatedFemale Spermatheca    49.6   NA  Ovary    99.9   NA  Testis    177   NA  Male Accessory Gland    95.8   39  Carcass    34.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    1 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    13 ovary, mated 4-day female    8 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    13 embryo 04-06hr    12 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    3 adult male 01day    6 adult male 05day    7 adult male 30day    6 adult female 01day    3 adult female 05day    7 adult female 30day    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2  (28) wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2  28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    10 embryonic Kc167    22 embryonic S1    4 embryonic S3    15 leg disc CME L1    4 wing disc CME-W2    28 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    5 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    11 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • LD04449 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033686 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12367-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hen1CB05312 Hen1EY10775 viable, with Scer\GAL4pnr-MD237 Hen1GD7706 Sterility Allele fertile Hen1EY10775 Phenotype manifest in Allele
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of Hen1 Class Mutagen Stocks Known lesion Hen15-HA-1264 1 -- Hen1e03039 piggyBac transposase 1 -- Hen1EY10775 P-element activity 1 -- Hen1EY22392 P-element activity 1 -- Hen1f00810 piggyBac transposase 1 Yes Hen1NP3512 1 -- Hen1CB05312 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hen1 Class Mutagen Stocks Known lesion Hen1GD7706 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hen1GL00078 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hen1HMS00262 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hen1KK100443 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hen1NIG.12367R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.12367R} NA P{TRiP.GL00078} NA P{TRiP.HMS00262} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{PTT-GB}Hen1CB05312 NA P{RS5}Hen15-HA-1264 NA PBac{RB}Hen1e03039 NA PBac{WH}Hen1f00810 NA insertion of enhancer trap binary system P{GawB}Hen1NP3512 No insertion of mobile activating element P{EPgy2}CG8878EY10775 NA P{EPgy2}Hen1EY22392 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
O-methyltransferase activity inferred from direct assay (Horwich et al., 2007, Saito et al., 2007)
Biological Process
CV term Evidence References
posttranscriptional gene silencing by RNA inferred from mutant phenotype (Horwich et al., 2007) RNA methylation inferred from direct assay (Horwich et al., 2007, Saito et al., 2007) inferred from mutant phenotype (Horwich et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
RNA methyltransferase activity inferred from electronic annotation with InterPro:IPR026610 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hen1 - CG9684 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Hen1 - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Hen1 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hen1 - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hen1 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033686 DroID - A comprehensive database of gene and protein interactions. • FBgn0033686 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36301
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6C88CK Dipteran inclusive ortholog search EOG6TTGSR Insect inclusive ortholog search EOG6H716V Arthropod inclusive ortholog search EOG6C2FRR Metazoa inclusive ortholog search EOG6NGVDZ
Orthologs in Drosophila Species (EOG6C88CK)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hen1     Drosophila simulans   Dsim\GD15398     Drosophila sechellia   Dsec\GM21335 Y   Drosophila erecta   Dere\GG20248 Y   Drosophila yakuba   Dyak\GE12407 Y   Drosophila ananassae   Dana\GF12293 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11585 Y   Drosophila persimilis   Dper\GL11079 Y   Drosophila willistoni   Dwil\GK23005 Y   Drosophila virilis   Dvir\GJ19904 Y   Drosophila mojavensis   Dmoj\GI20134 Y   Drosophila grimshawi   Dgri\GH19715 Y
Orthologs in non-Drosophila Dipterans (EOG6TTGSR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010029   Anopheles gambiae Malaria mosquito  Agam\AGAP005646   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004597   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016924
Orthologs in non-Dipteran Insects (EOG6H716V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49074   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG030239   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003673   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14878   Atta cephalotes Leafcutter ant  Acep\ACEP18399   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20565   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17326   Linepithema humile Argentine ant  Lhum\LH10160   Pogonomyrmex barbatus Red harvester ant  Pbar\CG:02550536   Solenopsis invicta Red fire ant  Sinv\SINV18041   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003345   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000386   Bombyx mori Silkmoth  Bmor\BGIBMGA007679   Pediculus humanus Human body louse  Phum\PHUM430970   Tribolium castaneum Red flour beetle  Tcas\TC004824
Orthologs in non-Insect Arthropods (EOG6C2FRR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW017730
Orthologs in non-Arthropod Metazoa (EOG6NGVDZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013747gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018269gn   Danio rerio Zebrafish  Drer\henmt1   Xenopus tropicalis Western clawed frog  Xtro\henmt1   Gallus gallus Domestic chicken  Ggal\HENMT1   Mus musculus House mouse  Mmus\Henmt1   Rattus norvegicus Norway rat  Rnor\Henmt1   Homo sapiens Human  Hsap\ENSG00000162639
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000162639
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM21335 Drosophila erecta Dere\GG20248 Drosophila yakuba Dyak\GE12407 Drosophila ananassae Dana\GF12293 Drosophila pseudoobscura pseudoobscura Dpse\GA11585 Drosophila persimilis Dper\GL11079 Drosophila willistoni Dwil\GK23005 Drosophila virilis Dvir\GJ19904 Drosophila mojavensis Dmoj\GI20134 Drosophila grimshawi Dgri\GH19715
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	22557 y1 w67c23; P{EPgy2}Hen1EY22392 35203 y1 sc* v1; P{TRiP.GL00078}attP2 33385 y1 sc* v1; P{TRiP.HMS00262}attP2 20213 y1 w67c23; P{EPgy2}CG8878EY10775 Hen1EY10775
Harvard	- PBac{RB}Hen1e03039 - PBac{WH}Hen1f00810
Kyoto	125171 w1118; P{RS5}Hen15-HA-1264 104528 y* w*; P{GawB}Hen1NP3512 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v103400 P{KK100443}VIE-260B v31920 w1118; P{GD7706}v31920 v31921 w1118; P{GD7706}v31921
Genomic Clones ( 1 )
	BACR04E21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD04449
Other clones	DPiM_LD04449
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE12904 SD21784
Other clones	214499.28 273982 CK01.44B.H1 CK01.88D.F9 CK01.91B.F6 EK037629 EK050963 EK069921 EK071455 EP21751 FGH123D03 RP001007766 RP001007931 RP001012541 RP001013551 RP001019045 RP001028846 RP001032311 RP001032925 RP001033248 RP001039501 RP001045080 RP001046120 RP001047241 RP001047405 RP001048054
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033686 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36301
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: hen1 CG12367 (Horwich et al., 2007) Source for identity of: Hen1 Pimet (FlyBase, 2009-) Source for identity of: Pimet hen1 (Saito et al., 2007)
Source for database merge of
Additional comments	FlyBase Curator comment: Renamed from 'Pimet/piRNA methyltransferase' to 'Hen1' because: i) the former is misleading because the gene product methylates both siRNAs and piRNAs in flies (see FBrf0202202); and ii) PubMed searches show that the wider research community clearly favours 'Hen1' over 'Pimet'. (FlyBase, 2009-)
Other Comments
	Production of mature siRNAs from endogenous long hairpin RNA genes is a hybrid mechanism that combines canonical RNA interference factors (Dcr-2, Hen1 and AGO2) and a canonical microRNA factor loqs. (Okamura et al., 2008) hen1 methylates the 3' termini of siRNAs and piRNAs. hen1 acts on single strands and not duplexes. (Horwich et al., 2007) hen1 is required for piRNA-directed silencing of transposable elements in the germline. (Horwich et al., 2007) Pimet shows single-stranded small RNA methylation activity in vitro. (Saito et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136888 NM_001273983 RefSeq (Proteins) • NP_610732 NP_001260912 DDBJ / EMBL / GenBank • AA201403 AAF58575 AAL39470 AGB93445 AW942054 AY069325 CL844358 CZ480807 CZ483293 Entrez Gene - A searchable database of RefSeq genes. • 36301 UniProtKB/Swiss-Prot • Q7K175
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • 3'-RNA ribose 2'-O-methyltransferase, Hen1 (IPR026610) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD04449
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0033686 DroID - A comprehensive database of gene and protein interactions. • FBgn0033686 DRSC - Results from RNAi screens. • FBgn0033686 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033686 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12367-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033686 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36301 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/hen1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36301 modMine - Data generated by the modENCODE project. • FBgn0033686
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG12367 (Horwich et al., 2007, Buszczak et al., 2007, Saito et al., 2007, Kawamura et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2010.12.1, Ni et al., 2011.7.19, Japanese National Institute of Genetics, 2012.5.21) DmHen1 (Horwich et al., 2007, Kawamura et al., 2008) FBgn 33686 (Lehmann et al., 2010) hen1 (Horwich et al., 2007, Okamura et al., 2008, Ameres et al., 2010, Ameres et al., 2011) Hen1 (Olivieri et al., 2010) hen-1 (Okamura et al., 2011) PIMET (Kolaczkowski et al., 2011) Pimet (Yu et al., 2011, Saito et al., 2007, Kawamura et al., 2008)
Name Synonym	CG12367   piRNA methyltransferase (Saito et al., 2007)
Secondary FlyBase IDs
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Website  ( 1 )
Recent research papers ( 5 )
	Lee and Langley, 2012, Genetics 192(4): 1411--1432 Long-term and short-term evolutionary impacts of transposable elements on Drosophila. [FBrf0220133] Ameres et al., 2011, RNA 17(1): 54--63 Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. [FBrf0212657] Kolaczkowski et al., 2011, Mol. Biol. Evol. 28(2): 1033--1042 Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. [FBrf0212798] Okamura et al., 2011, Mol. Cell. Biol. 31(4): 884--896 R2D2 Organizes Small Regulatory RNA Pathways in Drosophila. [FBrf0212879] Yu et al., 2011, PLoS Genet. 7(3): e1001340 Triplet Repeat-Derived siRNAs Enhance RNA-Mediated Toxicity in a Drosophila Model for Myotonic Dystrophy. [FBrf0213314] Show all research papers ...