Gene Dmel\hen1

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General Information

Symbol

Dmel\hen1

Species

D. melanogaster

Name

Annotation symbol

CG12367

Feature type

protein_coding_gene

FlyBase ID

FBgn0033686

Created / Updated

2003-12-02/2008-06-26

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

48E2-48E3

Sequence location

2R:8,033,231..8,040,259 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol hen1 (CG12367, FBgn0033686). It has the cytological map location 48E2-48E3. Its sequence location is 2R:8033231..8040259. Its molecular function is described as O-methyltransferase activity. It is involved in the biological processes: RNA methylation; posttranscriptional gene silencing by RNA. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48E2-48E3

Limits computationally determined from genome sequence between P{lacW}jeb^k05644 and P{PZ}SmD3⁰²⁸³³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\hen1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

hen1-RA

FBtr0087984

NM_136888

1559

391

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

hen1-PA

FBpp0087092

45.6

391

4.44

NP_610732

AAF58575

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033686

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7K175

Maps to

Does NOT map to

Identified with

LD04449

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12841

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033686

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033686

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033686

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Sterility

Allele

fertile

hen1^EY10775

Phenotype manifest in

Allele

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of hen1	Mutagen	Stocks	Known lesion
hen1^CB05312	P-element activity	0	Yes
hen1^EY10775	P-element activity	1	--
hen1^EY22392	P-element activity	1	--
hen1^e03039	piggyBac transposase	1	--
hen1^f00810	piggyBac transposase	1	Yes

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of hen1	Class	Mutagen	Stocks	Known lesion
hen1^GD7706		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful duplication

Dp(2;2)Y3b

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}hen1^e03039

PBac{WH}hen1^f00810

P{PTT-GB}hen1^CB05312

insertion of mobile activating element

P{EPgy2}CG8878^EY10775

P{EPgy2}hen1^EY22392

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

O-methyltransferase activity

inferred from direct assay

(Horwich et al., 2007)

Biological Process

CV term

Evidence

References

RNA methylation

inferred from mutant phenotype inferred from direct assay

(Horwich et al., 2007)

posttranscriptional gene silencing by RNA

inferred from mutant phenotype

(Horwich et al., 2007)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

hen1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62083

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12293

Dere\GG20248

Dgri\GH19715

Dmoj\GI20134

Dper\GL11079

Dpse\GA11585

Dsec\GM21335

Dvir\GJ19904

Dwil\GK23005

Dyak\GE12407

Curated drosophilid orthologs

Dpse\GA11585

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021705
Anopheles gambiae (Ensembl)
ENSCAFP00000029528
Canis familiaris (Ensembl)
NEWSINFRUP00000157426
Takifugu rubripes (Ensembl)
ENSGALP00000033585
Gallus gallus (Ensembl)
ENSP00000351932
Homo sapiens (Ensembl)
ENSMMUP00000012338
Macaca mulatta (Ensembl)
ENSMODP00000002874
Monodelphis domestica (Ensembl)
ENSPTRP00000001784
Pan troglodytes (Ensembl)
GSTENP00004980001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000011506
Xenopus tropicalis (Ensembl)
Q69U43
Oryza sativa (Gramene)
AT4G20910.1
Arabidopsis thaliana (Arabidopsis)
WBGene00036538
Caenorhabditis briggsae (Wormbase)
WBGene00015349
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-050417-388
Danio rerio (Zfin)
ZDB-GENE-050417-387
Danio rerio (Zfin)
MGI:1913965
Mus musculus (Informatics)
SPBC336.05c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Harvard

PBac{RB}CG12367^e03039

PBac{WH}CG12367^f00810

VDRC

v31920

w¹¹¹⁸; P{GD7706}v31920

v31921

w¹¹¹⁸; P{GD7706}v31921

Bloomington

22557

y¹ w^67c23; P{EPgy2}CG12367^EY22392

20213

y¹ w^67c23; P{EPgy2}CG8878^EY10775 CG12367^EY10775

Genomic Clones ( 1 )

BACR04E21

cDNA Clones ( 11 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD04449

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033686

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033686

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: hen1 CG12367

(Horwich et al., 2007)

Source for database merge of

Additional comments

Comments About Role

hen1 is required for piRNA-directed silencing of transposable elements in the germline.

(Horwich et al., 2007)

Comments About Molecular Function

hen1 methylates the 3' termini of siRNAs and piRNAs. hen1 acts on single strands and not duplexes.

(Horwich et al., 2007)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_136888

RefSeq (Proteins)

•

NP_610732

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q7K175

Other Crossreferences

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62083

DEDB - Drosophila exon database: splicing graphs

•

12841

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033686

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033686

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033686

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0033686

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033686

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033686

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021705
Anopheles gambiae (Ensembl)
ENSCAFP00000029528
Canis familiaris (Ensembl)
NEWSINFRUP00000157426
Takifugu rubripes (Ensembl)
ENSGALP00000033585
Gallus gallus (Ensembl)
ENSP00000351932
Homo sapiens (Ensembl)
ENSMMUP00000012338
Macaca mulatta (Ensembl)
ENSMODP00000002874
Monodelphis domestica (Ensembl)
ENSPTRP00000001784
Pan troglodytes (Ensembl)
GSTENP00004980001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000011506
Xenopus tropicalis (Ensembl)
Q69U43
Oryza sativa (Gramene)
AT4G20910.1
Arabidopsis thaliana (Arabidopsis)
WBGene00036538
Caenorhabditis briggsae (Wormbase)
WBGene00015349
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-050417-388
Danio rerio (Zfin)
ZDB-GENE-050417-387
Danio rerio (Zfin)
MGI:1913965
Mus musculus (Informatics)
SPBC336.05c
Schizosaccharomyces pombe (Sanger)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/genebrief/hen1.htm

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033686

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CG12367

(Horwich et al., 2007, Buszczak et al., 2007)

DmHen1

(Horwich et al., 2007)

hen1

(Horwich et al., 2007)

Name Synonym

CG12367

Secondary FlyBase IDs

References ( 9 )

Research paper

Buszczak et al., 2007, Genetics 175(3): 1505--1531: The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. [FBrf0200345]
Horwich et al., 2007, Curr. Biol. 17(14): 1265--1272: The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC. [FBrf0202202]
Metaxakis et al., 2005, Genetics 171(2): 571--581: Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Bellen, 2006.8.3, MBBatch686_2.xml: MBBatch686_2.xml [FBrf0191805]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.: Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]