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Gene Dmel\Prp8

General Information
Symbol	Dmel\Prp8	Species	D. melanogaster
Name	pre-mRNA processing factor 8	Annotation symbol	CG8877
Feature type	protein_coding_gene	FlyBase ID	FBgn0033688
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	48E3-48E4	Sequence location	2R:8,041,763..8,050,503 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pre-mRNA processing factor 8 is referred to in FlyBase by the symbol Dmel\Prp8 (CG8877, FBgn0033688). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mRNA splicing, via spliceosome; neurogenesis. 7 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: JAB1/Mov34/MPN/PAD-1; PRO, C-terminal; PROCN; PRP8 domain IV core; Pre-mRNA-processing-splicing factor 8; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding; Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding; RNA recognition motif, spliceosomal PrP8. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640679_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Prp8. Gene sequence location is 2R:8041763..8050503.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48E3-48E4   Limits computationally determined from genome sequence between P{lacW}jebk05644 and P{PZ}SmD302833
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2- (Coelho et al., 2005)
Left of (cM)	L (Coelho et al., 2005)
Right of (cM)	Bl (Coelho et al., 2005)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Prp8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Prp8-RA FBtr0088016  NM_136891   7442   2396
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Prp8-PA   FBpp0087124   279.6   2396   9.19     NP_610735   AAF58573
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • JAB1/Mov34/MPN/PAD-1 (IPR000555) Pre-mRNA-processing-splicing factor 8 (IPR012591) PROCN (IPR012592) PRO, C-terminal (IPR012984) Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding (IPR019580) Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding (IPR019581) RNA recognition motif, spliceosomal PrP8 (IPR019582) PRP8 domain IV core (IPR021983)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696449   AA696970   AA979077   AE013599 AAF58573   AI533757   AI534207   AI534949   AW943179   AY051826 AAK93250   BG639772   BI353448   BP543630   BQ103030   BT012296   CO179852   CO263705   CO268082   CO270743   CO276034   CO276363   CO276565   CO282639   CO287911   CO296507   CO300061   CO311253   CO312969   CO328546   CO330358   CO332976   CO337329   CO339383   CO342794   CO342931   CO343836   EC061774   EC065101   EC067418   EC076243   EC080472   EC085248   EC090700   EC213610   EC219851   EC219896   EC220197   EC220517   EC224758   EC230539   EC231505   EC236728   EC240725   EC243424   EC254796   EC259495   EC260541   EC260542   EC265024   EV585338   EV594041
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1Z8U0 Q960V2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (589.25) Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    589.25 Larval Midgut    96.5 Larval Hindgut    222.8 Larval Malpighian Tubules    177.7 Larval Fat Body    95.3 Larval Salivary Gland    366.9 Larval Trachea    291.55 Larval Carcass    238 Adult Head    94 Adult Eye    114.05 Adult Brain    143 Adult Thoracic-Abdominal Ganglion    121.7 Adult Crop    124.9 Adult Midgut    125.7 Adult Hindgut    131 Adult Malpighian Tubules    188 Adult Fat Body    126.4 Adult Salivary Gland    166.8 Adult Heart    113.3 Adult VirginFemale Spermatheca    118.3 Adult InseminatedFemale Spermatheca    145.3 Adult Ovary    478.7 Adult Testis    59 Adult Male Accessory Gland    186.7 Adult Carcass    107 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    94   NA  Eye    114.05   NA  Brain    143   589.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    121.7   NA  Crop    124.9   96.5  Midgut    125.7   222.8  Hindgut    131   177.7  Malpighian Tubules    188   95.3  Fat Body    126.4   366.9  Salivary Gland    166.8   NA  Heart    113.3   291.55  Trachea    NA   NA  VirginFemale Spermatheca    118.3   NA  InseminatedFemale Spermatheca    145.3   NA  Ovary    478.7   NA  Testis    59   NA  Male Accessory Gland    186.7   238  Carcass    107 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (49) central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering  (33) carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female  (64) ovary, mated 4-day female  (54) testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (49) central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering  (33) carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female  (64) ovary, mated 4-day female  (54) testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    49 central nervous system, pupae P8    20 head, virgin 1-day female    10 head, virgin 4-day female    8 head, virgin 20-day female    13 head, mated 1-day female    8 head, mated 4-day female    10 head, mated 20-day female    10 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    18 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    12 digestive system, 20-day adult    12 fat body, larvae L3 wandering    8 fat body, white prepupae    12 fat body, pupae P8    3 carcass, larvae L3 wandering    33 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    64 ovary, mated 4-day female    54 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr    154 embryo 06-08hr    116 embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (89) embryo 02-04hr  (89) embryo 04-06hr  (154) embryo 06-08hr  (116) embryo 08-10hr  (82) embryo 10-12hr  (44) embryo 12-14hr  (42) embryo 14-16hr  (35) embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1  (36) larva L2  (34) larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6  (30) larva L3 puffstage 7-9  (32) white prepupae new  (34) white prepupae 12hr  (35) white prepupae 24hr  (44) pupae 2d postWPP  26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day  (42) adult female 05day  (69) adult female 30day  (62) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr  (154) embryo 06-08hr  (116) embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr    154 embryo 06-08hr    116 embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr    154 embryo 06-08hr    116 embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (89) embryo 02-04hr  (89) embryo 04-06hr  (154) embryo 06-08hr  (116) embryo 08-10hr  (82) embryo 10-12hr  (44) embryo 12-14hr  (42) embryo 14-16hr  (35) embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1  (36) larva L2  (34) larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6  30 larva L3 puffstage 7-9  32 white prepupae new  (34) white prepupae 12hr  (35) white prepupae 24hr  (44) pupae 2d postWPP  26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day  (42) adult female 05day  (69) adult female 30day  (62) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr  (154) embryo 06-08hr  116 embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    89 embryo 02-04hr    89 embryo 04-06hr    154 embryo 06-08hr    116 embryo 08-10hr    82 embryo 10-12hr    44 embryo 12-14hr    42 embryo 14-16hr    35 embryo 16-18hr    22 embryo 18-20hr    12 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    36 larva L2    34 larva L3 12hr old    23 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    32 white prepupae new    34 white prepupae 12hr    35 white prepupae 24hr    44 pupae 2d postWPP    26 pupae 3d postWPP    12 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    14 adult male 30day    16 adult female 01day    42 adult female 05day    69 adult female 30day    62 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2    113 tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (83) Schneider line 2 Sg4  (37) embryonic 1182-4H  (57) embryonic GM2  (59) embryonic Kc167  (30) embryonic S1  (57) embryonic S3  (79) leg disc CME L1  (49) wing disc CME-W2  (53) wing disc ML-DmD8  (47) wing disc ML-DmD9  (79) wing disc ML-DmD16-c3  (59) wing disc ML-DmD21    19 wing disc ML-DmD32  (35) haltere disc ML-DmD17-c3  (105) eye-antennal disc ML-DmD11  (62) antennal disc ML-DmD20-c5  (37) mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (60) CNS ML-DmBG2-c2  (113) tumorous blood cells mbn2  (86) ovary fGS/OSS  (56) ovary OSC  (74) ovary OSS  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3  105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2  (113) tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2    113 tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2    113 tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (83) Schneider line 2 Sg4  (37) embryonic 1182-4H  (57) embryonic GM2  (59) embryonic Kc167  30 embryonic S1  (57) embryonic S3  (79) leg disc CME L1  (49) wing disc CME-W2  (53) wing disc ML-DmD8  (47) wing disc ML-DmD9  (79) wing disc ML-DmD16-c3  (59) wing disc ML-DmD21    19 wing disc ML-DmD32  (35) haltere disc ML-DmD17-c3  (105) eye-antennal disc ML-DmD11  (62) antennal disc ML-DmD20-c5  (37) mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (60) CNS ML-DmBG2-c2  (113) tumorous blood cells mbn2  (86) ovary fGS/OSS  (56) ovary OSC  (74) ovary OSS  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3  105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2  113 tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    83 Schneider line 2 Sg4    37 embryonic 1182-4H    57 embryonic GM2    59 embryonic Kc167    30 embryonic S1    57 embryonic S3    79 leg disc CME L1    49 wing disc CME-W2    53 wing disc ML-DmD8    47 wing disc ML-DmD9    79 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    19 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    105 eye-antennal disc ML-DmD11    62 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    60 CNS ML-DmBG2-c2    113 tumorous blood cells mbn2    86 ovary fGS/OSS    56 ovary OSC    74 ovary OSS    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (38) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (32) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (31) Paraquat 10 mM 48 hrs, 4-day adult  (36) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (38) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  31 Paraquat 10 mM 48 hrs, 4-day adult  (36) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    38 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    18 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    32 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033688 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8877-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Prp8GD6578 lethal, with Scer\GAL4pnr-MD237 Prp8GD6578 lethal | larval stage (with Prp82e1) Prp82e2 lethal | larval stage (with Prp82e2) Prp82e1 lethal | pupal stage, with Scer\GAL4dpp.PU Prp8GD6578 lethal | pupal stage | heat sensitive, with Scer\GAL4dpp.PU Prp8NIG.8877R partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Prp8GD6578 Other Phenotypes Allele increased cell death Prp8dsRNA.cRa neuroanatomy defective | larval stage Prp8GD6578 small body | larval stage (with Prp82e1) Prp82e2 small body | larval stage (with Prp82e2) Prp82e1 visible, with Scer\GAL4bbg-C96 Prp8NIG.8877R visible, with Scer\GAL4dpp.PU Prp8NIG.8877R visible, with Scer\GAL4pnr-MD237 Prp8GD6578 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage Prp8GD6578 ganglion mother cell | larval stage Prp8GD6578 mesothoracic tergum, with Scer\GAL4pnr-MD237 Prp8GD6578 scutellar bristle, with Scer\GAL4dpp.PU Prp8NIG.8877R wing, with Scer\GAL4bbg-C96 Prp8NIG.8877R
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Prp8 Class Mutagen Stocks Known lesion Prp8KG03188 2 -- Prp82e1 ethyl methanesulfonate 1 Yes Prp82e2 ethyl methanesulfonate 1 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Prp8 Class Mutagen Stocks Known lesion Prp8GD6578 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Prp8HMS01297 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Prp8dsRNA.cRa in vitro construct - RNAi 0 Yes Prp8NIG.8877R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC40 (Coelho et al., 2005)
Not disrupted in	Df(2R)CB21 (Coelho et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.8877R} NA P{TRiP.HMS01297} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}Prp8KG03188 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mRNA splicing, via spliceosome inferred from mutant phenotype (Andersen and Tapon, 2008) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mRNA splicing, via spliceosome inferred by curator from GO:0071011|GO:0071013 (Herold et al., 2009) inferred from sequence or structural similarity with NCBI:NP_006436 AND inferred from sequence or structural similarity with Saccharomyces PRP8 (Mount and Salz, 2000) inferred from sequence or structural similarity with Saccharomyces PRP8 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
spliceosomal complex inferred from sequence or structural similarity with Saccharomyces PRP8 (FlyBase, 1992-) U5 snRNP inferred from sequence or structural similarity with NCBI:NP_006436 AND inferred from sequence or structural similarity with Saccharomyces PRP8 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Prp8 - 5-HT7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - AttB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Bet3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Bx42 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prp8 - CG14259 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG14353 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG14641 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG15084 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG17187 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG1965 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prp8 - CG31413 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG3436 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG3511 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG3744 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG4338 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG4415 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG4825 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG4849 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG4973 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG5162 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG5190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG5516 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG6015 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG6034 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG6197 experimental knowledge based (Guruharsha et al., 2011) Prp8 - CG6652 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG6674 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prp8 - CG7137 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG7146 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG7686 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - CG9667 experimental knowledge based (Guruharsha et al., 2011) Prp8 - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Chi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Clc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Cyp6a21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - GalNAc-T1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Hakai anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Hph anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Irp-1A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Maf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Mfap1 anti bait coimmunoprecipitation, peptide massfingerprinting (Andersen and Tapon, 2008) Prp8 - Mob4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Nmt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Obp49a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Prp38 anti bait coimmunoprecipitation, peptide massfingerprinting (Andersen and Tapon, 2008) Prp8 - Pten anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - U2A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - Ugt86De anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - ap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - crn anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prp8 - ecd anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prp8 - elfless anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - grau anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - inaC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - l(1)10Bb experimental knowledge based (Guruharsha et al., 2011) Prp8 - l(3)72Ab experimental knowledge based (Guruharsha et al., 2011) Prp8 - lack anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - phl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - snf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - wee anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prp8 - yellow-f2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Prp8 allele Gene References unspecified Pi3K92E (Coelho et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62086 DroID - A comprehensive database of gene and protein interactions. • FBgn0033688 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36304
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6J11TC Dipteran inclusive ortholog search EOG6SXNMC Insect inclusive ortholog search EOG63TXQ8 Arthropod inclusive ortholog search EOG6MKKX2 Metazoa inclusive ortholog search EOG6TBH3X
Orthologs in Drosophila Species (EOG6J11TC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Prp8     Drosophila simulans   Dsim\GD15250 Y   Drosophila sechellia   Dsec\GM20383 Y   Drosophila erecta   Dere\GG22605 Y   Drosophila yakuba   Dyak\GE13473 Y   Drosophila ananassae   Dana\GF12641 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21384 Y   Drosophila persimilis   Dper\GL11026 Y   Drosophila willistoni   Dwil\GK23219 Y   Drosophila virilis   Dvir\GJ22442 Y   Drosophila mojavensis   Dmoj\GI19381 Y   Drosophila grimshawi   Dgri\GH22152 Y
Orthologs in non-Drosophila Dipterans (EOG6SXNMC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009959   Anopheles gambiae Malaria mosquito  Agam\AGAP006885   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016636
Orthologs in non-Dipteran Insects (EOG63TXQ8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45823   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004036   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17515   Atta cephalotes Leafcutter ant  Acep\ACEP12939   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19643   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23731   Linepithema humile Argentine ant  Lhum\LH12457   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18342   Solenopsis invicta Red fire ant  Sinv\SINV10803   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000739   Bombyx mori Silkmoth  Bmor\BGIBMGA009816   Pediculus humanus Human body louse  Phum\PHUM300700   Tribolium castaneum Red flour beetle  Tcas\GLEAN_10565
Orthologs in non-Insect Arthropods (EOG6MKKX2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW022487
Orthologs in non-Arthropod Metazoa (EOG6TBH3X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\prp-8   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017141gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021952gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028561gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012930gn   Danio rerio Zebrafish  Drer\prpf8   Xenopus tropicalis Western clawed frog  Xtro\prpf8   Gallus gallus Domestic chicken  Ggal\PRPF8   Mus musculus House mouse  Mmus\Prpf8   Rattus norvegicus Norway rat  Rnor\Prpf8   Homo sapiens Human  Hsap\PRPF8
Human Orthologs (1)
Gene OMIM HGNC Hsap\PRPF8
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15250 Drosophila sechellia Dsec\GM20383 Drosophila erecta Dere\GG22605 Drosophila yakuba Dyak\GE13473 Drosophila ananassae Dana\GF12641 Drosophila pseudoobscura pseudoobscura Dpse\GA21384 Drosophila persimilis Dper\GL11026 Drosophila willistoni Dwil\GK23219 Drosophila virilis Dvir\GJ22442 Drosophila mojavensis Dmoj\GI19381 Drosophila grimshawi Dgri\GH22152
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	34622 y1 sc* v1; P{TRiP.HMS01297}attP2 13006 y1; P{SUPor-P}Prp8KG03188/CyO; ry506 25905 w*; Prp82e1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 25912 w*; Prp82e2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
Kyoto	111506 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{SUPor-P}Prp8KG03188 /CyO y+
VDRC	v18565 w1118; P{GD6578}v18565 v18567 w1118; P{GD6578}v18567
Genomic Clones ( 2 )
	BACR04E21 BACR37I09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 54 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD33339 UT01590
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM07708 GM08818 GM23670 SD01244 SD06783
Other clones	562 39191 51290 80728 101438 102139 105558 191679 214249.25 214345.23 214379.18 214535.27 280565 542845 CK01.22C.B12 CK01.33A.H8 CK01.84C.B10 CK01.107A.A4 CK01.118D.E7 CK01.130C.G5 CK01.147B.G11 Dmel_dig_011832_0621_0571 Dmel_neb_009582_0634_2710 EC00351 EK067129 EK089334 EK090120 EK100121 EK119508 EK120628 EK121411 EK160901 EK177318 EK213841 EK223916 EK230829 EK290844 EK295040 EK305312 EN14305 EP01903 EP15524 EP15753 EP18206 FGC021E06 SD05715 UUGC0426
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033688 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36304
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: prp8 CG8877 (Coelho et al., 2005)
Source for database merge of
Additional comments	snoRNA:Prp8-a is encoded in an intron of Prp8. (Graveley et al., 2011) The mir-988 microRNA is derived from an intron of Prp8. (Kawamura et al., 2008)
Other Comments
	Identification: as a complementation group whose members dominantly modify the small wing phenotype caused by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096. 2 alleles of prp8 have been recovered. (Coelho et al., 2005) Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136891 RefSeq (Proteins) • NP_610735 DDBJ / EMBL / GenBank • AA979077 AAF58573 AAK93250 AW943179 AY051826 Entrez Gene - A searchable database of RefSeq genes. • 36304 UniProtKB/TrEMBL • A1Z8U0 Q960V2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • JAB1/Mov34/MPN/PAD-1 (IPR000555) Pre-mRNA-processing-splicing factor 8 (IPR012591) PROCN (IPR012592) PRO, C-terminal (IPR012984) Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding (IPR019580) Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding (IPR019581) RNA recognition motif, spliceosomal PrP8 (IPR019582) PRP8 domain IV core (IPR021983)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62086 DroID - A comprehensive database of gene and protein interactions. • FBgn0033688 DRSC - Results from RNAi screens. • FBgn0033688 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033688 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8877-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033688 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36304 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36304 modMine - Data generated by the modENCODE project. • FBgn0033688
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG8877 (Coelho et al., 2005, Mount and Salz, 2000, Ramet et al., 2002, Lai et al., 2003, Giot et al., 2003, Holloway et al., 2008, Nybakken et al., 2005, Lee et al., 2010, Andersen and Tapon, 2008, Zhang et al., 2012, Herold et al., 2009, Tsou et al., 2012, Ni et al., 2010.12.1) E-2e (Coelho et al., 2005) prp8 (Holloway et al., 2008, Lecuyer et al., 2007, Kawamura et al., 2008, Andersen and Tapon, 2008, Herold et al., 2009, Graveley et al., 2011, Neumüller et al., 2011, Japanese National Institute of Genetics, 2012.5.21) Prp8 (Somma et al., 2008, Zhang et al., 2012, Tsou et al., 2012, Graveley et al., 2011, Dekanty et al., 2010)
Name Synonym	pre-mRNA processing factor 8   Prp8 (Somma et al., 2008) prp8   U5 small nuclear ribonucleoprotein-specific factor (Lee et al., 2010)
Secondary FlyBase IDs
References ( 32 )
Generate a list of	All references relating to this gene ( 32 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 9 ) Personal communication to FlyBase  ( 4 ) FlyBase analysis  ( 2 ) Stock list  ( 1 )
Recent research papers ( 3 )
	Tsou et al., 2012, PLoS ONE 7(8): e43112 Systematic Analysis of the Physiological Importance of Deubiquitinating Enzymes. [FBrf0219256] Graveley et al., 2011, Nature 471(7339): 473--479 The developmental transcriptome of Drosophila melanogaster. [FBrf0213330] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...