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Gene Dmel\garz

General Information
Symbol	Dmel\garz	Species	D. melanogaster
Name	gartenzwerg	Annotation symbol	CG8487
Feature type	protein_coding_gene	FlyBase ID	FBgn0033714
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	CG 8487
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48F8-48F10	Sequence location	2R:8,214,179..8,221,324 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene gartenzwerg is referred to in FlyBase by the symbol Dmel\garz (CG8487, FBgn0033714). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: guanyl-nucleotide exchange factor activity. There is experimental evidence that it is involved in the biological process: epithelial cell development; cell morphogenesis; salivary gland development; phagocytosis, engulfment. 6 alleles are reported. The phenotypes of these alleles are annotated with: rhabdomere; eye; intersegmental nerve; eye photoreceptor cell. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: SEC7-like; SEC7-like, alpha orthogonal bundle. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval trachea, adult female reproductive system, adult testis, larval/adult carcass. Gene sequence location is 2R:8214179..8221324.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References D'Ambrosio and Vale, 2010, J. Cell Biol. 191(3): 471--478 Sequence features BKN20511 HFA07193 Controlled Vocabulary Terms cell morphogenesis
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48F8-48F10   Limits computationally determined from genome sequence between P{EP}garzEP2028 and P{lacW}l(2)k11404k11404
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\garz for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone AT08349 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) garz-RA FBtr0087898  NM_165881   5317   1740 garz-RB FBtr0087897  NM_136917   6275   1983 garz-RC FBtr0305075     5320   1741
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein garz-PA   FBpp0087011   194.9   1740   5.66     NP_725133   AAM68666 garz-PB   FBpp0087010   220.6   1983   5.08     NP_610761   AAF58532 garz-PC   FBpp0293612   194.9   1741   5.66
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • SEC7-like (IPR000904) SEC7-like, alpha orthogonal bundle (IPR023394)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58532   AE013599 AAM68666   AI544273   AI544282   AQ074040   AY089287 AAL90025   BF486864   BF506003   BG636614   CO179680   CO262592   CO266965   CO271204   CO271206   CO306188   CO321848   CO338916   CO338989   CO341581   EC084341   EC196543   EC196544   EC206088   EC211737   EC226359   EC230400   EC236827   EC244738   EC244976   EC251653   EC252207   EC254245   EC255442   EL884992   EL885116   EL885243   EV591451   EV595562   EY198883   GH957510   GH964009   GH967005   GH969394   GH983452
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8T4A1 A1Z8W9 A1Z8W8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0033714    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0033714 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0033714 expression level Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1922) embryo 02-04hr  (684) embryo 04-06hr  (1548) embryo 06-08hr  (2260) embryo 08-10hr  (2185) embryo 10-12hr  (2036) embryo 12-14hr  (1989) embryo 14-16hr  (1934) embryo 16-18hr  (1593) embryo 18-20hr  (1244) embryo 20-22hr  (1066) embryo 22-24hr  (1033) larva L1  (1311) larva L2  (1865) larva L3 12hr old  (1447) larva L3 puffstage 1-2  (900) larva L3 puffstage 3-6  (1236) larva L3 puffstage 7-9  (1267) white prepupae new  (1407) white prepupae 12hr  (1331) white prepupae 24hr  (1904) pupae 2d postWPP  (1738) pupae 3d postWPP  (1486) pupae 4d postWPP  (1279) adult male 01day  (2109) adult male 05day  (2611) adult male 30day  (2645) adult female 01day  (1333) adult female 05day  (1822) adult female 30day  (1820) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1922) embryo 02-04hr    684 embryo 04-06hr  (1548) embryo 06-08hr  (2260) embryo 08-10hr  (2185) embryo 10-12hr  (2036) embryo 12-14hr  (1989) embryo 14-16hr  (1934) embryo 16-18hr  (1593) embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2  (1865) larva L3 12hr old  1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new  1407 white prepupae 12hr    1331 white prepupae 24hr  (1904) pupae 2d postWPP  (1738) pupae 3d postWPP  1486 pupae 4d postWPP    1279 adult male 01day  (2109) adult male 05day  (2611) adult male 30day  (2645) adult female 01day    1333 adult female 05day  (1822) adult female 30day  (1820) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0033714 expression level Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1922) embryo 02-04hr  (684) embryo 04-06hr  (1548) embryo 06-08hr  (2260) embryo 08-10hr  (2185) embryo 10-12hr  (2036) embryo 12-14hr  (1989) embryo 14-16hr  (1934) embryo 16-18hr  (1593) embryo 18-20hr  (1244) embryo 20-22hr  (1066) embryo 22-24hr  (1033) larva L1  (1311) larva L2  (1865) larva L3 12hr old  (1447) larva L3 puffstage 1-2  (900) larva L3 puffstage 3-6  (1236) larva L3 puffstage 7-9  (1267) white prepupae new  (1407) white prepupae 12hr  (1331) white prepupae 24hr  (1904) pupae 2d postWPP  (1738) pupae 3d postWPP  (1486) pupae 4d postWPP  (1279) adult male 01day  (2109) adult male 05day  (2611) adult male 30day  (2645) adult female 01day  (1333) adult female 05day  (1822) adult female 30day  (1820) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1922 embryo 02-04hr    684 embryo 04-06hr  1548 embryo 06-08hr  2260 embryo 08-10hr  2185 embryo 10-12hr  2036 embryo 12-14hr  1989 embryo 14-16hr  1934 embryo 16-18hr  1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2  1865 larva L3 12hr old  1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new  1407 white prepupae 12hr    1331 white prepupae 24hr  1904 pupae 2d postWPP  1738 pupae 3d postWPP  1486 pupae 4d postWPP    1279 adult male 01day  2109 adult male 05day  (2611) adult male 30day  (2645) adult female 01day    1333 adult female 05day  1822 adult female 30day  1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1922 embryo 02-04hr    684 embryo 04-06hr    1548 embryo 06-08hr    2260 embryo 08-10hr    2185 embryo 10-12hr    2036 embryo 12-14hr    1989 embryo 14-16hr    1934 embryo 16-18hr    1593 embryo 18-20hr    1244 embryo 20-22hr    1066 embryo 22-24hr    1033 larva L1    1311 larva L2    1865 larva L3 12hr old    1447 larva L3 puffstage 1-2    900 larva L3 puffstage 3-6    1236 larva L3 puffstage 7-9    1267 white prepupae new    1407 white prepupae 12hr    1331 white prepupae 24hr    1904 pupae 2d postWPP    1738 pupae 3d postWPP    1486 pupae 4d postWPP    1279 adult male 01day    2109 adult male 05day    2611 adult male 30day    2645 adult female 01day    1333 adult female 05day    1822 adult female 30day    1820 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0033714    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0033714 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval trachea, adult female reproductive system, adult testis, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033714 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8487-RB;CG8487-RA;CG8487-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for garz Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 garzGD13940 lethal | pupal stage, with Scer\GAL4pnr-MD237 garzGD13940 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 garzEP2028 visible, with Scer\GAL4GMR.PF garzEP2028 Phenotype manifest in Allele anterior fascicle & synapse, with Scer\GAL4elav-C155 garzEP2028 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 garzEP2028 eye, with Scer\GAL4GMR.PF garzEP2028 eye photoreceptor cell, with Scer\GAL4GMR.PF garzEP2028 intersegmental nerve, with Scer\GAL4elav-C155 garzEP2028 rhabdomere, with Scer\GAL4GMR.PF garzEP2028
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of garz Class Mutagen Stocks Known lesion garzEP2028 P-element activity 2 Yes garzMB05159 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of garz Class Mutagen Stocks Known lesion garzGD13940 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes garzHMS01397 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes garzJF01603 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes garzdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6061 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13940} NA P{TRiP.HMS01397} NA P{TRiP.JF01603} NA P{UAS-garz.dsRNA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}garzEP2028 NA insertion of enhancer trap binary system Mi{ET1}garzMB05159 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell morphogenesis inferred from mutant phenotype (D'Ambrosio and Vale, 2010) epithelial cell development inferred from direct assay (Szul et al., 2011) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) salivary gland development inferred from direct assay (Szul et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ARF guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR000904 (FlyBase Curators et al., 2004-) guanyl-nucleotide exchange factor activity inferred from sequence or structural similarity with Saccharomyces GEA1 AND inferred from sequence or structural similarity with UniProtKB:Q92538 (FlyBase, 1992-)
Biological Process
CV term Evidence References
ER to Golgi vesicle-mediated transport inferred from sequence or structural similarity with Saccharomyces GEA1 (FlyBase, 1992-) intra-Golgi vesicle-mediated transport inferred from sequence or structural similarity with Saccharomyces GEA1 (FlyBase, 1992-) regulation of ARF protein signal transduction inferred from electronic annotation with InterPro:IPR000904 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
Golgi-associated vesicle inferred from sequence or structural similarity with Saccharomyces GEA1 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions garz allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62114 DroID - A comprehensive database of gene and protein interactions. • FBgn0033714 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36337
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12843 Dere\GG20274 Dgri\GH23159 Dmoj\GI18338 Dper\GL10823 Dpse\GA21111 Dsec\GM21360 Dvir\GJ19764 Dwil\GK22165 Dyak\GE12432
Curated drosophilid orthologs	Dpse\GA21111
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL009678-PA Aedes aegypti (mosquito) AGAP001527-PA Anopheles gambiae (mosquito) XP_001123021.1 Apis mellifera (honey bee) XP_001948659.1 Acyrthosiphon pisum (pea aphid) At1g13980.1 Arabidopsis thaliana (wall cress) 90433 Batrachochytrium dendrobatidis (chytrid fungus) 133420 Branchiostoma floridae (lancelet) XP_001894516.1 Brugia malayi (roundworm nematode) CN12473 Caenorhabditis brenneri (small nematode) CBP33104 Caenorhabditis briggsae (nematode) CE41612 Caenorhabditis elegans (worm) ENSCAFP00000014886 Canis familiaris (dog) CAGL0I03102g Candida glabrata (haploid yeast) ENSCINP00000008886 Ciona intestinalis (sea squirt) JA11926 Caenorhabditis japonica (nematode) cn03221 Cryptococcus neoformans (yeast-like fungus) cgd4_2690 Cryptosporidium parvum (protozoan parasite) CPIJ001197 Culex pipens (mosquito) ENSCPOP00000004072 Cavia porcellus (guinea pig) 152758 Chlamidomonas reinhardtii (green alga) RP36957 Caenorhabditis remanei (nematode) ENSCSAVP00000014427 Ciona savignyi (sedentary tunicate) 227129 Capitella spl (polychaete worm) DDB0233595 Dictyostelium discoideum (amoeba) DEHA2A11946g Debaryomyces Hansenii (yeast) 197351 Daphnia pulex (daphnia) ENSDARP00000058171 Danio rerio (zebra fish) ENSECAP00000021486 Equus caballus (horse) ENSGACP00000019290 Gymnopilus aculeatus (mushroom) ENSGALP00000009030 Gallus gallus (chicken) 77812 Hypsipyla robusta (cedar shoot borer) ENSP00000359000 Homo sapiens (human) KLLA0E22727g Kluyveromyces lactis (yeast) 130403 Lottia gigantea (owl limpet) LmjF34.2450 Leishmania major (virus) 35734 Monocosta brevicollis (choanoflagellate) ENSMODP00000014221 Monodelphis domestica (gray opossum) ENSMMUP00000003992 Macaca mulatta (rhesus monkey) ENSMUSP00000026254 Mus musculus (house mouse) 136901 Nematostella vectensis (starlet anemone) XP_001601088.1 Nasonia vitripennis (wasp) ENSORLP00000001406 Oryzias latipes (japanese killifish) LOC_Os02g22090.1 Oryza sativa (asian rice) 197 Ostreococcus tauri (green alga) PHUM000233-PA Pediculus humanus (human body louse) PP12111 Pristionchus pacificus (nematode) 175438 Physcomitrella patens (moss) ENSPPYP00000003022 Pongo pygmaeus (orangutan) 78649 Phytophthora ramorum (sudden oak death pathogen) 135443 Phytophthora sojae (root rot pathogen) 258967 Populus trichocarpa (black cottonwood) ENSPTRP00000039661 Pan troglodytes (chimpanzee) ENSRNOP00000038037 Rattus norvegicus (norway rat) 4996977 Sorghum bicolor (sorghum) YEL022W Saccharomyces cerevisiae (budding yeast) Smp_101280 Schistosoma mansoni (trematode parasite) SPBC211.03c Schizosaccharomyces pombe (fission yeast) XP_001193947.1 Strongylocentrotus purpuratus (purple sea urchin) 24514 Trichoplax adhaerens (metazoa) XP_967092.2 Tribolium castaneum (red flour beetle) Tc00.1047053506559.440 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000006894 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008395 Takifugu rubripes (pufferfish Takifugu) 80483.m00086 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000022082 Xenopus tropicalis (frog) YALI0E19536g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11036 LH22858 ISCW012910 FBgn0230175 FBgn0224150 FBgn0206906 FBgn0182619 FBgn0182618 FBgn0176241 JGI_V11_197351 FBgn0081099 FBgn0148433 FBgn0141077 FBgn0130616 FBgn0112465 FBgn0089877 CPIJ001197 BGIBMGA007380 ACYPI000942 GB12561 AGAP001527 AAEL009678 PB23857 PHUM494780 GLEAN_05830
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	24324 w1118; Mi{ET1}garzMB05159 17017 w1118; P{EP}garzEP2028/CyO 31232 y1 v1; P{TRiP.JF01603}attP2 34987 y1 sc* v1; P{TRiP.HMS01397}attP2/TM3, Sb1
Kyoto	114444 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EP}garzEP2028 /CyO y+
VDRC	v42140 w1118; P{GD13940}v42140 v42141 w1118; P{GD13940}v42141/CyO
Genomic Clones ( 2 )
	BACR37I09 BACR37P06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT08349
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT21413 SD10766 SD10778 SD14360
Other clones	33430 33472 36829 60292 102800 147228 214219.23 214371.29 214441.30 214475.2 CK01.10A.D11 CK01.77A.G1 CK01.101D.F8 Dmel_neb_011204_0632_2419 Dmel_neb_013714_0326_1091 EK060225 EK085856 EK102319 EK102322 EK148041 EK186522 EK250458 EP00262 EP00527 EP12732 IP16188 IP16288 IP16388 RP004057216 RP004209705 RP004442100 RP004473555 RP004509000
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033714 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36337
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: garz CG8487 (Kraut, 2002.3.6)
Source for database merge of
Additional comments
Other Comments
	garz depletion inhibits AP-1 recruitment to membranes. (Szul et al., 2011) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) dsRNA made from templates generated with primers directed against garz that is transfected into S2 treated with Listeria monocytogenes reveals garz to be involved in Listeria monocytogenes entry. (Cheng et al., 2005) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Identification: in a screen for genes which when overexpressed cause motorneuron pathfinding and or synaptogenesis errors. (Kraut and Zinn, 2001) Involved in endocytosis/membrane trafficking. (Kraut and Zinn, 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136917 NM_165881 RefSeq (Proteins) • NP_610761 NP_725133 DDBJ / EMBL / GenBank • AAF58532 AAL90025 AAM68666 AQ074040 AY089287 BF506003 Entrez Gene - A searchable database of RefSeq genes. • 36337 UniProtKB/TrEMBL • A1Z8W8 A1Z8W9 Q8T4A1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • SEC7-like (IPR000904) SEC7-like, alpha orthogonal bundle (IPR023394)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62114 DroID - A comprehensive database of gene and protein interactions. • FBgn0033714 DRSC - Results from RNAi screens. • FBgn0033714 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033714 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8487-RB;CG8487-RA;CG8487-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033714 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36337 InParanoid A subset of ortholog calls from InParanoid. • AAEL009678-PA Aedes aegypti (mosquito) AGAP001527-PA Anopheles gambiae (mosquito) XP_001123021.1 Apis mellifera (honey bee) XP_001948659.1 Acyrthosiphon pisum (pea aphid) At1g13980.1 Arabidopsis thaliana (wall cress) 90433 Batrachochytrium dendrobatidis (chytrid fungus) 133420 Branchiostoma floridae (lancelet) XP_001894516.1 Brugia malayi (roundworm nematode) CN12473 Caenorhabditis brenneri (small nematode) CBP33104 Caenorhabditis briggsae (nematode) CE41612 Caenorhabditis elegans (worm) ENSCAFP00000014886 Canis familiaris (dog) CAGL0I03102g Candida glabrata (haploid yeast) ENSCINP00000008886 Ciona intestinalis (sea squirt) JA11926 Caenorhabditis japonica (nematode) cn03221 Cryptococcus neoformans (yeast-like fungus) cgd4_2690 Cryptosporidium parvum (protozoan parasite) CPIJ001197 Culex pipens (mosquito) ENSCPOP00000004072 Cavia porcellus (guinea pig) 152758 Chlamidomonas reinhardtii (green alga) RP36957 Caenorhabditis remanei (nematode) ENSCSAVP00000014427 Ciona savignyi (sedentary tunicate) 227129 Capitella spl (polychaete worm) DDB0233595 Dictyostelium discoideum (amoeba) DEHA2A11946g Debaryomyces Hansenii (yeast) 197351 Daphnia pulex (daphnia) ENSDARP00000058171 Danio rerio (zebra fish) ENSECAP00000021486 Equus caballus (horse) ENSGACP00000019290 Gymnopilus aculeatus (mushroom) ENSGALP00000009030 Gallus gallus (chicken) 77812 Hypsipyla robusta (cedar shoot borer) ENSP00000359000 Homo sapiens (human) KLLA0E22727g Kluyveromyces lactis (yeast) 130403 Lottia gigantea (owl limpet) LmjF34.2450 Leishmania major (virus) 35734 Monocosta brevicollis (choanoflagellate) ENSMODP00000014221 Monodelphis domestica (gray opossum) ENSMMUP00000003992 Macaca mulatta (rhesus monkey) ENSMUSP00000026254 Mus musculus (house mouse) 136901 Nematostella vectensis (starlet anemone) XP_001601088.1 Nasonia vitripennis (wasp) ENSORLP00000001406 Oryzias latipes (japanese killifish) LOC_Os02g22090.1 Oryza sativa (asian rice) 197 Ostreococcus tauri (green alga) PHUM000233-PA Pediculus humanus (human body louse) PP12111 Pristionchus pacificus (nematode) 175438 Physcomitrella patens (moss) ENSPPYP00000003022 Pongo pygmaeus (orangutan) 78649 Phytophthora ramorum (sudden oak death pathogen) 135443 Phytophthora sojae (root rot pathogen) 258967 Populus trichocarpa (black cottonwood) ENSPTRP00000039661 Pan troglodytes (chimpanzee) ENSRNOP00000038037 Rattus norvegicus (norway rat) 4996977 Sorghum bicolor (sorghum) YEL022W Saccharomyces cerevisiae (budding yeast) Smp_101280 Schistosoma mansoni (trematode parasite) SPBC211.03c Schizosaccharomyces pombe (fission yeast) XP_001193947.1 Strongylocentrotus purpuratus (purple sea urchin) 24514 Trichoplax adhaerens (metazoa) XP_967092.2 Tribolium castaneum (red flour beetle) Tc00.1047053506559.440 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000006894 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008395 Takifugu rubripes (pufferfish Takifugu) 80483.m00086 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000022082 Xenopus tropicalis (frog) YALI0E19536g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36337 modMine - Data generated by the modENCODE project. • FBgn0033714 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11036 LH22858 ISCW012910 FBgn0230175 FBgn0224150 FBgn0206906 FBgn0182619 FBgn0182618 FBgn0176241 JGI_V11_197351 FBgn0081099 FBgn0148433 FBgn0141077 FBgn0130616 FBgn0112465 FBgn0089877 CPIJ001197 BGIBMGA007380 ACYPI000942 GB12561 AGAP001527 AAEL009678 PB23857 PHUM494780 GLEAN_05830
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG8487 (Cox et al., 2004, Shulman and Feany, 2003, Gupta et al., 2009, Ni et al., 2008, Ni et al., 2011.7.19) CG 8487 (Kraut et al., 2001, Kraut et al., 2001) garz (Stroschein-Stevenson et al., 2006, Wendler et al., 2010, Bakal et al., 2007, Gan et al., 2010, Gabut et al., 2007, Guo et al., 2008, Raghu et al., 2009, Telonis-Scott et al., 2009, Gupta et al., 2009, McIntyre et al., 2006, Szul et al., 2011) Garz (D'Ambrosio and Vale, 2010) GBF1 (Cox et al., 2004)
Name Synonym	gartenzwerg (Kraut, 2002.3.6, Gabut et al., 2007, Gupta et al., 2009) Gartenzwerg (Kraut and Zinn, 2001, Szul et al., 2011) sec7-family GEF (Kraut et al., 2001, Kraut et al., 2001)
Secondary FlyBase IDs
	FBgn0063599
References ( 36 )
Generate a list of	All references relating to this gene ( 36 )
List References by type	Research paper  ( 17 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 5 )
	Szul et al., 2011, Cell. Logist. 1(2): 69--76 The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila. [FBrf0213963] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] D'Ambrosio and Vale, 2010, J. Cell Biol. 191(3): 471--478 A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. [FBrf0212211] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Wendler et al., 2010, EMBO J. 29(2): 304--314 A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway. [FBrf0209739] Show all research papers ...