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Gene Dmel\dgt5

General Information
Symbol	Dmel\dgt5	Species	D. melanogaster
Name	dim γ-tubulin 5	Annotation symbol	CG8828
Feature type	protein_coding_gene	FlyBase ID	FBgn0033740
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	49A7-49A7	Sequence location	2R:8,353,183..8,359,280 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene dim γ-tubulin 5 is referred to in FlyBase by the symbol Dmel\dgt5 (CG8828, FBgn0033740). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: regulation of mitosis; mitotic spindle organization. 6 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1641436_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of dgt5. Gene sequence location is 2R:8353183..8359280.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 49A7-49A7   Limits computationally determined from genome sequence between P{lacW}l(2)k11404k11404 and P{lacW}fdlk17040
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\dgt5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) dgt5-RA FBtr0087948  NM_136941   2300   685 dgt5-RB FBtr0339638     2213   685
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein dgt5-PA   FBpp0087059   78.0   685   8.79     NP_610785   AAF58505 dgt5-PB   FBpp0308700   78.0   685   8.79
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142145   AE013599 AAF58505   AI061733   AI257591   AI258670   AI515590   AI518864   AQ073632   AW943906   AY052018 AAK93442   BF488347   BF495879   BF500559   BF501093   BI230441   BI230537   BI352775   BI358571   BI635813   BP557210   CO311430   CO315574   CO330958   EC090399   EC225342   EC249364   EV579208   GH942953   GH943047   GH944195   GH945390   GH948289   GH952038   GH953161   GH953221   GH954184   GH954562   GH955602   GH956670   GH956942   GH957458   GH957871   GH958157   GH958273   GH958895   GH959645   GH960203   GH961469   GH961560   GH962964   GH963629   GH963908   GH964143   GH966763   GH967085   GH969073   GH970224   GH970423   GH970470   GH971690   GH972802   GH973470   GH973966   GH974319   GH975433   GH975759   GH976526   GH977893   GH979179   GH979634   GH980310   GH980402   GH982008   GH982446   GH982579   GH982916
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K4B4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with HAUS complex inferred from direct assay (Guruharsha et al., 2011) microtubule associated complex inferred from direct assay (Hughes et al., 2008) colocalizes_with spindle inferred from direct assay (Goshima et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    112 Larval Midgut    2.4 Larval Hindgut    17.9 Larval Malpighian Tubules    4.4 Larval Fat Body    14.4 Larval Salivary Gland    7.3 Larval Trachea    58.175 Larval Carcass    17.725 Adult Head    9.9 Adult Eye    17.25 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.2 Adult Crop    5.5 Adult Midgut    7.7 Adult Hindgut    5 Adult Malpighian Tubules    3.6 Adult Fat Body    4.6 Adult Salivary Gland    2.9 Adult Heart    10.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    6.9 Adult Ovary    228.9 Adult Testis    29 Adult Male Accessory Gland    2.5 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    9.9   NA  Eye    no informative data   NA  Brain    5.2   112  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    8.2   NA  Crop    5.5   2.4  Midgut    7.7   17.9  Hindgut    5   4.4  Malpighian Tubules    3.6   no informative data  Fat Body    4.6   7.3  Salivary Gland    2.9   NA  Heart    no informative data   58.175  Trachea    NA   NA  VirginFemale Spermatheca    4.6   NA  InseminatedFemale Spermatheca    6.9   NA  Ovary    228.9   NA  Testis    29   NA  Male Accessory Gland    2.5   no informative data  Carcass    7.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    6 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    23 testis, mated 4-day male    4 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    21 embryo 04-06hr    23 embryo 06-08hr    16 embryo 08-10hr    14 embryo 10-12hr    6 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    6 adult female 05day    20 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    14 embryonic 1182-4H    14 embryonic GM2    13 embryonic Kc167    21 embryonic S1    15 embryonic S3    23 leg disc CME L1    15 wing disc CME-W2    19 wing disc ML-DmD8    11 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    22 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    14 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • LD47477 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033740 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8828-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 dgt5GD13788 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of dgt5 Class Mutagen Stocks Known lesion dgt5EP2492 P-element activity 1 -- dgt5DG02709 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of dgt5 Class Mutagen Stocks Known lesion dgt5GD13788 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes dgt5MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes dgt5MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes dgt5NIG.8828R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)achi4 (Ayyar et al., 2003)
Not disrupted in	Df(2R)achi2 (Ayyar et al., 2003) Df(2R)achi3 (Ayyar et al., 2003)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.8828R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}dgt5EP2492 NA miscellaneous insertions P{wHy}dgt5DG02709 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007, Goshima et al., 2007, Hughes et al., 2008) regulation of mitosis inferred from mutant phenotype (Goshima et al., 2007)
Cellular Component
CV term Evidence References
colocalizes_with HAUS complex inferred from direct assay (Guruharsha et al., 2011) microtubule associated complex inferred from direct assay (Hughes et al., 2008) colocalizes_with spindle inferred from direct assay (Goshima et al., 2007)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
dgt5 - dgt2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) dgt5 - dgt3 experimental knowledge based (Guruharsha et al., 2011) dgt5 - dgt4 experimental knowledge based (Guruharsha et al., 2011) dgt5 - dgt6 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) dgt5 - msd1 experimental knowledge based (Guruharsha et al., 2011) dgt5 - msd5 experimental knowledge based (Guruharsha et al., 2011) dgt5 - wac experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions dgt5 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0033740
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6DZ2DX Dipteran inclusive ortholog search EOG6K0QWR Insect inclusive ortholog search EOG67D8B9 Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6DZ2DX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\dgt5     Drosophila simulans   Dsim\GD25837 Y   Drosophila sechellia   Dsec\GM20358 Y   Drosophila erecta   Dere\GG22576 Y   Drosophila yakuba   Dyak\GE13445 Y   Drosophila ananassae   Dana\GF12450 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21351 Y   Drosophila persimilis   Dper\GL17348 Y   Drosophila willistoni   Dwil\GK22108 Y   Drosophila virilis   Dvir\GJ15044 Y   Drosophila mojavensis   Dmoj\GI19874 Y   Drosophila grimshawi   Dgri\GH19917 Y
Orthologs in non-Drosophila Dipterans (EOG6K0QWR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003088   Anopheles gambiae Malaria mosquito  Agam\AGAP005732   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000015
Orthologs in non-Dipteran Insects (EOG67D8B9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Acyrthosiphon pisum Pea aphid  Apim\ACYPI004342   Acyrthosiphon pisum Pea aphid  Apim\ACYPI087321
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25837 Drosophila sechellia Dsec\GM20358 Drosophila erecta Dere\GG22576 Drosophila yakuba Dyak\GE13445 Drosophila ananassae Dana\GF12450 Drosophila pseudoobscura pseudoobscura Dpse\GA21351 Drosophila persimilis Dper\GL17348 Drosophila willistoni Dwil\GK22108 Drosophila virilis Dvir\GJ15044 Drosophila mojavensis Dmoj\GI19874 Drosophila grimshawi Dgri\GH19917
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	17062 w1118; P{EP}dgt5EP2492
VDRC	v26911 w1118; P{GD13788}v26911 v26912 w1118; P{GD13788}v26912
Genomic Clones ( 2 )
	BACR24H09 BACR37P06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 72 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47477
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT09540 AT15542 AT16313 AT23588 GM20723 LD34823 LD35277 LP01917 LP05996 RE14122 RE14235 RE45386 SD17312
Other clones	102945 147834 193591 CK00305 Dmel_dig_002550_0706_3162 EK224536 EK261901 EK296711 FGM308A02 RP004020093 RP004038241 RP004041282 RP004048234 RP004057731 RP004068716 RP004074009 RP004094814 RP004112339 RP004118310 RP004132889 RP004133855 RP004150939 RP004197703 RP004215681 RP004219499 RP004220976 RP004226484 RP004230846 RP004241902 RP004242451 RP004242952 RP004248001 RP004252806 RP004255045 RP004256730 RP004274081 RP004317649 RP004335495 RP004342786 RP004366380 RP004372870 RP004380973 RP004391774 RP004408647 RP004426867 RP004427259 RP004429583 RP004446417 RP004472047 RP004475043 RP004507854 RP004509455 RP004513312 RP004519367 RP004533059 RP004535203 RP004547720 RP004554986
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033740 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36364
Antibody Information
	polyclonal (Goshima et al., 2008)
Other Information
Discoverer
Etymology
	Named "dim γ-tubulin 5" for the decreased γ-tubulin staining of the spindle pole following treatment of S2 cells with dgt5 dsRNA. (Goshima et al., 2007)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the failure of γ-tubulin to localize to the spindle, without affecting its localization to the pole, and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the production of long spindles with low microtubule density, misaligned chromosomes/kinetochores and mitotic delay when assayed in S2 cells. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136941 RefSeq (Proteins) • NP_610785 DDBJ / EMBL / GenBank • AAF58505 AAK93442 AI515590 AQ073632 AW943906 AY052018 Entrez Gene - A searchable database of RefSeq genes. • 36364 UniProtKB/TrEMBL • Q7K4B4
Other Crossreferences
	BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD47477
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0033740 DRSC - Results from RNAi screens. • FBgn0033740 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033740 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8828-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033740 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36364 modMine - Data generated by the modENCODE project. • FBgn0033740
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG8828 (Goshima et al., 2007, Hughes et al., 2008, Japanese National Institute of Genetics, 2012.5.21) Dgt5 (Goshima et al., 2007, Goshima et al., 2007, Goshima et al., 2008, Meireles et al., 2009, Wainman et al., 2009, Bucciarelli et al., 2009) dgt5 (Guruharsha et al., 2011) msd2 (Hughes et al., 2008)
Name Synonym	CG8828   dim γ-tubulin 5   mitotic spindle density 2 (Hughes et al., 2008)
Secondary FlyBase IDs
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Guruharsha et al., 2011, Cell 147(3): 690--703 A Protein Complex Network of Drosophila melanogaster. [FBrf0216491] Show all research papers ...