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Gene Dmel\ClC-b

General Information
Symbol	Dmel\ClC-b	Species	D. melanogaster
Name	Chloride channel-b	Annotation symbol	CG8594
Feature type	protein_coding_gene	FlyBase ID	FBgn0033755
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	49B5-49B5	Sequence location	2R:8,459,573..8,462,804 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Chloride channel-b is referred to in FlyBase by the symbol Dmel\ClC-b (CG8594, FBgn0033755). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: chloride channel activity. There is experimental evidence that it is involved in the biological process: sensory perception of touch. 5 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Chloride channel, core; Chloride channel, voltage gated; Cystathionine beta-synthase, core. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 12-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637558_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8594. Gene sequence location is 2R:8459573..8462804.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms sensory perception of touch
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 49B5-49B5   Limits computationally determined from genome sequence between P{EP}CG8569EP1182 and P{EP}AmphEP2175&P{lacW}spt4k05316
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ClC-b for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ClC-b-RA FBtr0087826  NM_136954   2647   813
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ClC-b-PA   FBpp0086939   90.5   813   8.48     NP_610798   AAF58489
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cystathionine beta-synthase, core (IPR000644) Chloride channel, voltage gated (IPR001807) Chloride channel, core (IPR014743)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA948986   AE013599 AAF58489   AY071581 AAL49203   BI375798   CA803529   CA807585   CO177451   CO269894   EC231732   EC257233
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JZ25
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    349.875 Larval Midgut    191.2 Larval Hindgut    177.7 Larval Malpighian Tubules    250.2 Larval Fat Body    366.3 Larval Salivary Gland    163.7 Larval Trachea    180.275 Larval Carcass    161.05 Adult Head    211.7 Adult Eye    157.875 Adult Brain    310.5 Adult Thoracic-Abdominal Ganglion    285.6 Adult Crop    291 Adult Midgut    164.7 Adult Hindgut    169.4 Adult Malpighian Tubules    130.8 Adult Fat Body    282.1 Adult Salivary Gland    173.4 Adult Heart    223.9 Adult VirginFemale Spermatheca    237.7 Adult InseminatedFemale Spermatheca    215.1 Adult Ovary    213.8 Adult Testis    55.1 Adult Male Accessory Gland    291.4 Adult Carcass    180.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    211.7   NA  Eye    157.875   NA  Brain    310.5   349.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    285.6   NA  Crop    291   191.2  Midgut    164.7   177.7  Hindgut    169.4   250.2  Malpighian Tubules    130.8   366.3  Fat Body    282.1   163.7  Salivary Gland    173.4   NA  Heart    223.9   180.275  Trachea    NA   NA  VirginFemale Spermatheca    237.7   NA  InseminatedFemale Spermatheca    215.1   NA  Ovary    213.8   NA  Testis    55.1   NA  Male Accessory Gland    291.4   161.05  Carcass    180.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    13 central nervous system, pupae P8    18 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    6 head, mated 1-day male    6 head, mated 4-day male    8 head, mated 20-day male    11 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    16 ovary, mated 4-day female    10 testis, mated 4-day male    4 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 12-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (28) embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9  (29) white prepupae new  (34) white prepupae 12hr  (39) white prepupae 24hr  (31) pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9  29 white prepupae new  (34) white prepupae 12hr  (39) white prepupae 24hr  31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    15 embryo 12-14hr    19 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    7 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    29 white prepupae new    34 white prepupae 12hr    39 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    13 adult male 05day    13 adult male 30day    11 adult female 01day    13 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (44) Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2  (36) embryonic Kc167  (35) embryonic S1  (29) embryonic S3  (38) leg disc CME L1    15 wing disc CME-W2  (31) wing disc ML-DmD8  (30) wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3  (39) eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5  (29) mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2  (57) tumorous blood cells mbn2  (33) ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (44) Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2  (36) embryonic Kc167  (35) embryonic S1  29 embryonic S3  (38) leg disc CME L1    15 wing disc CME-W2  31 wing disc ML-DmD8  30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3  (39) eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5  29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2  (57) tumorous blood cells mbn2  (33) ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    36 embryonic Kc167    35 embryonic S1    29 embryonic S3    38 leg disc CME L1    15 wing disc CME-W2    31 wing disc ML-DmD8    30 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    39 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    29 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    57 tumorous blood cells mbn2    33 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_23
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033755 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8594-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Dcr-2Scer\UAS.cDa, Scer\GAL4e22c, Scer\GAL4sqh.PW ClC-bGD1958 viable, with Dcr-2Scer\UAS.cDa, Scer\GAL4e22c, Scer\GAL4sqh.PW ClC-bKK100840 viable, with Scer\GAL4pnr-MD237 ClC-bGD1958 Other Phenotypes Allele touch sensitivity defective, with Scer\GAL4109(2)80 ClC-bGD1958 touch sensitivity defective, with Scer\GAL41003.3 ClC-bGD1958 touch sensitivity defective | larval stage, with Dcr-2Scer\UAS.cDa, Scer\GAL41003.3 ClC-bGD1958 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of ClC-b Class Mutagen Stocks Known lesion ClC-bG2453 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ClC-b Class Mutagen Stocks Known lesion ClC-bGD1958 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ClC-bJF01844 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ClC-bKK100840 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ClC-bNIG.8594R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7121 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.8594R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}ClC-bG2453 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
sensory perception of touch inferred from mutant phenotype (Tsubouchi et al., 2012)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
chloride channel activity inferred from sequence or structural similarity with UniProtKB:P51799 (FlyBase, 1992-) voltage-gated chloride channel activity inferred from electronic annotation with InterPro:IPR001807 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
chloride transport inferred from electronic annotation with InterPro:IPR001807 (FlyBase Curators et al., 2004-) transmembrane transport inferred from electronic annotation with InterPro:IPR001807, InterPro:IPR014743 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR001807 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ClC-b - Hip1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ClC-b allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62155 DroID - A comprehensive database of gene and protein interactions. • FBgn0033755 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36381
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6PNZ2C Dipteran inclusive ortholog search EOG6FR10P Insect inclusive ortholog search EOG65MM19 Arthropod inclusive ortholog search EOG62Z355 Metazoa inclusive ortholog search EOG6PCPK1
Orthologs in Drosophila Species (EOG6PNZ2C)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ClC-b     Drosophila simulans   Dsim\GD10902 Y   Drosophila sechellia   Dsec\GM21405 Y   Drosophila erecta   Dere\GG20318 Y   Drosophila yakuba   Dyak\GE12477 Y   Drosophila ananassae   Dana\GF13396 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21190 Y   Drosophila persimilis   Dper\GL21306 Y   Drosophila willistoni   Dwil\GK23152 Y   Drosophila virilis   Dvir\GJ22257 Y   Drosophila mojavensis   Dmoj\GI19125 Y   Drosophila grimshawi   Dgri\GH22038 Y
Orthologs in non-Drosophila Dipterans (EOG6FR10P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007547   Anopheles gambiae Malaria mosquito  Agam\AGAP007499   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008618
Orthologs in non-Dipteran Insects (EOG65MM19)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB51847   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008840   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH25342   Atta cephalotes Leafcutter ant  Acep\ACEP15439   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17481   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21642   Linepithema humile Argentine ant  Lhum\LH15942   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18157   Solenopsis invicta Red fire ant  Sinv\SINV13878   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003562   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000431   Bombyx mori Silkmoth  Bmor\BGIBMGA005600   Pediculus humanus Human body louse  Phum\PHUM429700   Tribolium castaneum Red flour beetle  Tcas\TC005310
Orthologs in non-Insect Arthropods (EOG62Z355)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_303513   Ixodes scapularis Deer tick  Isca\ISCW001866
Orthologs in non-Arthropod Metazoa (EOG6PCPK1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\clh-6   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006772gn   Danio rerio Zebrafish  Drer\clcn7   Xenopus tropicalis Western clawed frog  Xtro\clcn7   Gallus gallus Domestic chicken  Ggal\CLCN7   Mus musculus House mouse  Mmus\Clcn7   Rattus norvegicus Norway rat  Rnor\Clcn7   Homo sapiens Human  Hsap\CLCN7
Human Orthologs (1)
Gene OMIM HGNC Hsap\CLCN7
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10902 Drosophila sechellia Dsec\GM21405 Drosophila erecta Dere\GG20318 Drosophila yakuba Dyak\GE12477 Drosophila ananassae Dana\GF13396 Drosophila pseudoobscura pseudoobscura Dpse\GA21190 Drosophila persimilis Dper\GL21306 Drosophila willistoni Dwil\GK23152 Drosophila virilis Dvir\GJ22257 Drosophila mojavensis Dmoj\GI19125 Drosophila grimshawi Dgri\GH22038
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	25826 y1 v1; P{TRiP.JF01844}attP2 26996 y1 w*; P{EP}ClC-bG2453
VDRC	v103420 P{KK100840}VIE-260B v4642 w1118; P{GD1958}v4642/TM3
Genomic Clones ( 1 )
	BACR24H09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE63672
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD27710
Other clones	214481.21 CK01.125C.E10 EK053528 EK096251 ESG011b.E21_H10 ESG018c.E21_E06
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033755 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36381
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ClC-b CG8594 (Rose et al., 2007)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136954 RefSeq (Proteins) • NP_610798 DDBJ / EMBL / GenBank • AAF58489 AAL49203 AY071581 BI375798 Entrez Gene - A searchable database of RefSeq genes. • 36381 UniProtKB/TrEMBL • Q7JZ25
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cystathionine beta-synthase, core (IPR000644) Chloride channel, voltage gated (IPR001807) Chloride channel, core (IPR014743)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62155 DroID - A comprehensive database of gene and protein interactions. • FBgn0033755 DRSC - Results from RNAi screens. • FBgn0033755 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033755 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8594-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033755 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36381 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36381 modMine - Data generated by the modENCODE project. • FBgn0033755
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG8594 (Wang et al., 2004, Rose et al., 2007, Perkins et al., 1.15.2009, Keleman et al., 2009.8.5, Tsubouchi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lotti et al., 2012) ClC-b (Tsubouchi et al., 2012) Clcn7 (Lotti et al., 2012) DmClCb (Rose et al., 2007) DmClC-b (Rose et al., 2007)
Name Synonym	Chloride channel-b
Secondary FlyBase IDs
References ( 19 )
Research paper	Lotti et al., 2012, Cell 151(2): 440--454 An SMN-Dependent U12 Splicing Event Essential for Motor Circuit Function. [FBrf0219670] Tsubouchi et al., 2012, Curr. Biol. 22(22): 2124--2134 Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae. [FBrf0219946] Rose et al., 2007, J. Exp. Biol. 210(14): 2489--2500 Properties and possible function of a hyperpolarisation-activated chloride current in Drosophila. [FBrf0202184] Wang et al., 2004, Genome Biol. 5(9): R69 Function-informed transcriptome analysis of Drosophila renal tubule. [FBrf0180324]
Supplementary material	Tsubouchi et al., 2012, Curr. Biol. 22(22): Tables S1 and S2. RNAi Lines Tested for Screening and Candidate Channel Genes, Related to Figure 5. [FBrf0220657] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Bellen, 2009.3.16, BDGP_2009_0316.GenExel.xml BDGP_2009_0316.GenExel.xml [FBrf0207422] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452] Perkins et al., 1.15.2009, Initial TRiP stock collection. Initial TRiP stock collection. [FBrf0206489] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Curated genome annotation	Ketchum, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126677]