FlyBase Gene Report: Dmel\CG12374

General Information

Symbol

Dmel\CG12374

Species

D. melanogaster

Name

Annotation Symbol

CG12374

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033774

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

49C3-49C3

Sequence location

2R:12,762,113..12,763,825 [+]

Recombination map

2-67

RefSeq locus

NT_033778 REGION:12762113..12763825

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables metallocarboxypeptidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000194073

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR000834

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from biological aspect of ancestor with PANTHER:PTN000194073

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000194073

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

M14 METALLOCARBOXYPEPTIDASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

M14 METALLOCARBOXYPEPTIDASES -: M14 metallopeptidases belong to MEROPS family M14. They hydrolyse single, C-terminal amino acids from polypeptide chains and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Their functions are numerous and diverse, and include digestion of food and processing of bioactive peptides. (Adapted from FBrf0239469.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG12374 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7JYV3)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG12374-RA

FBtr0087854

NM_136975

1464

422

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG12374-PA

FBpp0086967

48.1

422

4.68

NP_610819

AAF58456

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG12374 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG12374_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

dorsal sensory complex primordium

(Fisher et al., 2012)

posterior spiracle primordium

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG12374 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG12374

View exonic expression by tissue for Dmel\CG12374

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033774

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36410/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033774

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033774

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033774

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12374

Flygut - An atlas of the Drosophila adult midgut

FBgn0033774

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG12374

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG12374

Transgenic constructs containing regulatory region of CG12374

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG12374^GD5758

viable, with Scer\GAL4^Mef2.PR

CG12374^GD5758

viable, with Scer\GAL4^pnr-MD237

CG12374^GD5758

viable, with Scer\GAL4^tin.CΔ4

CG12374^GD5758

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CPA2

8 of 15

Yes

7 of 15

7 of 15

7 of 15

7 of 15

6 of 15

6 of 15

6 of 15

5 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cpa2

8 of 15

Yes

7 of 15

7 of 15

7 of 15

7 of 15

7 of 15

7 of 15

6 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (12)

Rnor\Cpa2

8 of 13

Yes

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

5 of 13

2 of 13

Yes

Rnor\Agbl2

1 of 13

Rnor\Agbl5

1 of 13

Rnor\Agtpbp1

1 of 13

Xenopus tropicalis (Western clawed frog) (14)

Xtro\cpb2

6 of 12

Yes

Xtro\cpa1

5 of 12

Xtro\cpa3

5 of 12

Xtro\cpb1

5 of 12

Yes

5 of 12

5 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (11)

Drer\cpa2

7 of 15

Yes

Drer\cpa4

6 of 15

Yes

6 of 15

6 of 15

6 of 15

6 of 15

6 of 15

5 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\T06A4.1

6 of 15

Yes

Cele\ZC434.9

6 of 15

Yes

5 of 15

4 of 15

4 of 15

4 of 15

4 of 15

3 of 15

3 of 15

1 of 15

1 of 15

1 of 15

Yes

Cele\sdc-3

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT5G42320

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ECM14

7 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC337.07c

5 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (21)

Gene Symbol

Score

Source

Alignment

7 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

5 of 10

4 of 10

3 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CPA2; carboxypeptidase A2

8 of 15

600688

CPA1; carboxypeptidase A1

7 of 15

114850

CPA4; carboxypeptidase A4

7 of 15

607635

CPA5; carboxypeptidase A5

7 of 15

609561

CPB1; carboxypeptidase B1

7 of 15

114852

CPA6; carboxypeptidase A6

6 of 15

609562

CPB2; carboxypeptidase B2

6 of 15

603101

CPO; carboxypeptidase O

6 of 15

609563

CPA3; carboxypeptidase A3

5 of 15

114851

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62182

DroID - A comprehensive database of gene and protein interactions.

FBgn0033774

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of Complement cascade

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-67

Cytogenetic map

49C3-49C3

Sequence location

2R:12,762,113..12,763,825 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49C3-49C3

Limits computationally determined from genome sequence between P{PZ}Nacα⁰⁴³²⁹ and P{lacW}bic^k10712

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-67

(FlyBase, 2016)

2-70.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

VDRC

v101566

P{KK103857}VIE-260B

v13919

w¹¹¹⁸; P{GD5758}v13919

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (66)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH58587

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033774

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GH01170
GH01542
GH01555
GH02372
GH03117
GH03879
GH05092
GH05357
GH06012
GH07215
GH10484
GH12914
GH13711
GH14254
GH14379
GH14390
GH17531
GH17815
GH18965
GH19395
GH19818
GH20302
GH20522
GH20579
GH20587
GH23215
GH23257
GH24026
GH25013
GH26585
RH17578
RH17651
RH43696
RH60859
RH62681
RH66324

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033774

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36410

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG12374 anon-WO0140519.63

(FlyBase, 1996-)

Additional comments

Source for merge of CG12374 anon-WO0140519.63 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36410

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7JYV3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7JYV3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7JYV3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033774

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033774

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36410/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033774

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG12374_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033774

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033774

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12374

Flygut - An atlas of the Drosophila adult midgut

FBgn0033774

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36410

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12374

MARRVEL_MODEL - MARRVEL (model organism gene)

36410

modMine - A data warehouse for the modENCODE project

FBgn0033774

Linkouts

BioGRID - A database of protein and genetic interactions.

62182

DroID - A comprehensive database of gene and protein interactions.

FBgn0033774

DRSC - Results frm RNAi screens

FBgn0033774

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033774

FlyMine - An integrated database for Drosophila genomics

FBgn0033774

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of Complement cascade

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG12374

(Hung et al., 2020, Bost et al., 2018, Garlapow et al., 2017, Mossman et al., 2016, Palu and Thummel, 2016, Sitnik et al., 2016, Li et al., 2015, Bischof and FlyORF project members, 2014.6.20, Brown et al., 2014, Ruiz et al., 2014, Wilson et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, McGraw et al., 2008)

anon-WO0140519.63

Name Synonyms

Secondary FlyBase IDs

FBgn0044565

Datasets (7)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (7)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (43)

The following external sites may use different assemblies or annotations than FlyBase.

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