Gene Dmel\CG13322

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General Information

Symbol

Dmel\CG13322

Species

D. melanogaster

Name

CG13322

Annotation symbol

CG13322

Feature type

protein_coding_gene

FlyBase ID

FBgn0033784

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

49E1-49E1

Sequence location

2R:8,821,732..8,824,032 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG13322 (FBgn0033784). It has the cytological map location 49E1. Its sequence location is 2R:8821732..8824032. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49E1-49E1

Limits computationally determined from genome sequence between P{PZ}l(2)01424⁰¹⁴²⁴ and P{lacW}Psc^k07834&P{lacW}Su(z)2^k06344

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG13322 for information on other features

Comments on Gene Model

EST data support existence of multiple transcripts with same CDS due to variable use of 5' exons

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG13322-RA

FBtr0087805

NM_136984

2033

334

CG13322-RB

FBtr0087804

NM_165962

2050

334

CG13322-RC

FBtr0087803

NM_165963

2104

334

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG13322-PA

FBpp0086918

38.0

334

4.36

NP_610828

AAM68618

CG13322-PB

FBpp0086917

38.0

334

4.36

NP_725243

AAM68619

CG13322-PC

FBpp0086916

38.0

334

4.36

NP_725244

AAF58439

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033784

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein of unknown function DUF298 (IPR005176)

Defective in cullin neddylation (IPR014764)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8T8S1

Maps to

Does NOT map to

Identified with

AT31457

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12727

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA942305

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033784

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033784

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033784

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG13322	Class	Mutagen	Stocks	Known lesion
CG13322^KG04872			0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG13322	Class	Mutagen	Stocks	Known lesion
CG13322^GD7981		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)vg-D

Useful duplication

Dp(2;2)M14

Disrupted in

Df(2R)Exel7123

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7981}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}CG13322^KG04872

insertion of mobile activating element

P{EP}EP2057

P{EP}EP2391

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 0 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG13322 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62196

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11995

Dere\GG22539

Dgri\GH21603

Dmoj\GI19574

Dper\GL17317

Dpse\GA12204

Dsec\GM20324

Dsim\GD25802

Dvir\GJ21150

Dwil\GK20876

Dyak\GE13410

Curated drosophilid orthologs

Dpse\GA12204

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021558
Anopheles gambiae (Ensembl)
ENSBTAP00000008459
Bos taurus (Ensembl)
ENSCAFP00000026384
Canis familiaris (Ensembl)
ENSCINP00000015600
Ciona intestinalis (Ensembl)
NEWSINFRUP00000141646
Takifugu rubripes (Ensembl)
ENSGALP00000003552
Gallus gallus (Ensembl)
ENSMODP00000008768
Monodelphis domestica (Ensembl)
ENSRNOP00000019167
Rattus norvegicus (Ensembl)
GSTENP00024095001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000036490
Xenopus tropicalis (Ensembl)
MGI:2679003
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

v32179

w¹¹¹⁸; P{GD7981}v32179

v32178

w¹¹¹⁸; P{GD7981}v32178/CyO

Bloomington

14107

y¹ w^67c23; P{SUPor-P}KG04872

Genomic Clones ( 1 )

BACR30D19

cDNA Clones ( 19 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT31457

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033784

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033784

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG13322 anon-WO0118547.137 Source for merge of CG13322 anon-WO0118547.137 was sequence comparison (date:051113).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

Area matching Drosophila EST AA942305.

(Davies, 2001.3.15)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q8T8S1

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein of unknown function DUF298 (IPR005176)

Defective in cullin neddylation (IPR014764)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62196

DEDB - Drosophila exon database: splicing graphs

•

12727

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0033784

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033784

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0033784

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0033784

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0033784

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0033784

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021558
Anopheles gambiae (Ensembl)
ENSBTAP00000008459
Bos taurus (Ensembl)
ENSCAFP00000026384
Canis familiaris (Ensembl)
ENSCINP00000015600
Ciona intestinalis (Ensembl)
NEWSINFRUP00000141646
Takifugu rubripes (Ensembl)
ENSGALP00000003552
Gallus gallus (Ensembl)
ENSMODP00000008768
Monodelphis domestica (Ensembl)
ENSRNOP00000019167
Rattus norvegicus (Ensembl)
GSTENP00024095001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000036490
Xenopus tropicalis (Ensembl)
MGI:2679003
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0033784

University of Chicago Dev. Expression

•

FBgn0033784

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

anon-WO0118547.137

CG13322

Name Synonym

CG13322

Secondary FlyBase IDs

FBgn0062222

References ( 10 )

Supplementary material

Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

FlyBase analysis

Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.: Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Davies, 2001.3.15, GenBank/EMBL/DDBJ: AX094013: Sequence 137 from Patent WO0118547. [FBrf0149821]

Patent

Davies et al., 2001, World Patent 0118547:: Essential genes and assays relating thereto. [FBrf0199103]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]