FB2019_05, released Oct 15, 2019

Gene: Dmel\GLaz

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General Information
Symbol
Dmel\GLaz
Species
D. melanogaster
Name
Glial Lazarillo
Annotation Symbol
CG4604
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033799
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Snapshot
Glial Lazarillo (GLaz) encodes a lipid binding protein of the Lipocalin family, which are small extracellular proteins with a single-domain beta-barrel structure. It is expressed in subsets of central and peripheral glial cells and in hemocytes. It regulates longevity, stress resistance, and behavior. [Date last reviewed: 2018-10-11]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
49F4-49F4
Sequence location
2R:13,163,959..13,168,032 [-]
Recombination map
2-68
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\GLaz or the JBrowse view of Dmel\GLaz for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.42
Gene model reviewed during 5.51
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
GLaz-RA
FBtr0087772
NM_079003
1018
212
GLaz-RB
FBtr0310151
NM_001274007
1001
212
GLaz-RC
FBtr0310152
NM_001274008
1120
212
GLaz-RD
FBtr0310153
NM_001274009
1023
212
GLaz-RE
FBtr0310154
NM_001274010
1084
212
GLaz-RF
FBtr0310155
NM_001274011
1101
212
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
GLaz-PA
FBpp0086885
24.0
212
8.84
NP_523727
AAF58418
GLaz-PB
FBpp0301836
24.0
212
8.84
NP_001260936
AGB93469
GLaz-PC
FBpp0301837
24.0
212
8.84
NP_001260937
AGB93470
GLaz-PD
FBpp0301838
24.0
212
8.84
NP_001260938
AGB93471
GLaz-PE
FBpp0301839
24.0
212
8.84
NP_001260939
AGB93472
GLaz-PF
FBpp0301840
24.0
212
8.84
NP_001260940
AGB93473
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

212 aa isoforms: GLaz-PA, GLaz-PB, GLaz-PC, GLaz-PD, GLaz-PE, GLaz-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GLaz using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (8 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
cellular response to oxidative stress
inferred from mutant phenotype
(Sanchez et al., 2006, Walker et al., 2006)
inferred from direct assay
(Muffat et al., 2008)
cellular response to starvation
inferred from mutant phenotype
(Sanchez et al., 2006, Walker et al., 2006)
determination of adult lifespan
inferred from mutant phenotype
(Sanchez et al., 2006, Walker et al., 2006)
lipid catabolic process
inferred from genetic interaction with FLYBASE:fh; FB:FBgn0030092
(Navarro et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
lipid metabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000064702
(assigned by GO_Central )
(Gaudet et al., 2011)
response to reactive oxygen species
inferred from biological aspect of ancestor with PANTHER:PTN000064702
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
extracellular space
inferred from direct assay
(Sanchez et al., 2000)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000064702
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
salivary gland body primordium
(Sanchez et al., 2000)
anal pad primordium
(Sanchez et al., 2000)
posterior spiracle primordium
(Sanchez et al., 2000)
clypeo-labral primordium | restricted
(Sanchez et al., 2000)
embryonic stage 4 -- 5
organism | faint
(Sanchez et al., 2000)
embryonic stage 9 -- 10
hindgut proper primordium
(Sanchez et al., 2000)
visceral mesoderm
(Sanchez et al., 2000)
amnioserosa | faint
(Sanchez et al., 2000)
embryonic stage 11 -- 13
embryonic hindgut
(Sanchez et al., 2000)
embryonic stage 12 -- 16
interface glial cell
(Sanchez et al., 2000)
embryonic stage 13 -- 14
midgut primordium | restricted

Comment: at foregut/midgut and midgut/hindgut boundaries

(Sanchez et al., 2000)
embryonic stage 15 -- 16
embryonic midgut constriction
(Sanchez et al., 2000)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 17
organism

Comment: expression peaks at 0-2 and 16-24 hrs AEL

(Sanchez et al., 2000)
second instar larval stage
organism

Comment: very weak expression

(Sanchez et al., 2000)
pupal stage -- adult stage
organism

Comment: strong expression

(Sanchez et al., 2000)
Additional Descriptive Data
GLaz transcript is expressed in a subset of glial cells in the embryonic CNS from embryonic stages 12-16. Expression is confined mainly to the neuropil, and is localized to a symmetrical chain of cells aligned to the longitudinal connectives. At stages 13-16, GLaz transcript is expressed in 8 cells/hemineuromere; morphology and location indicate these are interface glial cells. There is no PNS expression. Outside of the CNS, GLaz transcript is expressed at a low level in blastoderm stage embryos. At embryonic stage 9-10, GLaz is expressed in the visceral mesoderm, and weakly in the amnioserosa. At stages 11-13, expression is observed in the developing hindgut, and at stages 13-14 in epithelial cells at the boundaries between the midgut and hindgut, and midgut and foregut. Late in stage 14, GLaz is expressed at the sites of the future midgut constrictions, then later in the midgut constrictions. Expression is also observed in the primordia of the salivary glands, anal pads and posterior spiracles, and a subset of cells in the clypeo-labral primordium.
(Sanchez et al., 2000)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
extracellular space
inferred from direct assay
(Sanchez et al., 2000)
Expression Deduced from Reporters
Reporter: Mi{Trojan-GAL4.0}GLazMI02243-TG4.0
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
pigment cell
(Liu et al., 2017)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\GLaz in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of GLaz
Transgenic constructs containing regulatory region of GLaz
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\APOD
4 of 15
Yes
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Apod
4 of 15
Yes
No
Rattus norvegicus (Norway rat) (1)
Rnor\Apod
3 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (3)
Drer\apoda.2
5 of 15
Yes
No
Drer\apodb
5 of 15
Yes
No
Drer\apoda.1
4 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\lpr-4
1 of 15
Yes
Yes
Cele\lpr-6
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\TIL
2 of 9
Yes
No
Atha\CHL
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190EU4 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\GLaz
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002466
Drosophila simulans
Dsim\GD18610
Drosophila sechellia
Dsec\GM20309
Drosophila erecta
Dere\GG22523
Drosophila yakuba
Dyak\GE13396
Drosophila ananassae
Dana\GF11979
Drosophila pseudoobscura pseudoobscura
Dpse\GA18293
Drosophila persimilis
Dper\GL10843
Drosophila willistoni
Dwil\GK21632
Drosophila virilis
Dvir\GJ21134
Drosophila mojavensis
Dmoj\GI19559
Drosophila grimshawi
Dgri\GH21588
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AJG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17027529
Glossina morsitans
Tsetse fly
Gmor\GMOY006625
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03226
Mayetiola destructor
Hessian fly
Mdes\Mdes003154
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011278
Anopheles gambiae
Malaria mosquito
Agam\AGAP005223
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0FDB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA002890
Danaus plexippus
Monarch butterfly
Dple\DPOGS212432-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012357
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10015
Tribolium castaneum
Red flour beetle
Tcas\TC034333
Pediculus humanus
Human body louse
Phum\PHUM220050
Rhodnius prolixus
Kissing bug
Rpro\RPRC010099
Cimex lectularius
Bed bug
Clec\CLEC003887
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI22402
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0FCS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tetranychus urticae
Two-spotted spider mite
Turt\tetur24g01030
Tetranychus urticae
Two-spotted spider mite
Turt\tetur37g00940
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_230361
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
NLaz
4 of 10
CG31659
2 of 10
CG31446
1 of 10
Karl
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    GLazUAS.cWa
    ameliorates  Friedreich ataxia
    modeled by fhdsRNA.IR.UAS
    (Navarro et al., 2010)
    modeled by fhdsRNA.UAS.cLa
    (Navarro et al., 2010)
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    APOD; apolipoprotein D
    4 of 15
    107740
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        fh
        suppressible
        GLaz
        (Navarro et al., 2010)
        Marf
        suppressible
        GLaz
        (Liu et al., 2017)
        ND-42
        suppressible
        GLaz
        (Liu et al., 2017)
        sicily
        suppressible
        GLaz
        (Liu et al., 2017)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Gene Group - Pathway Membership (FlyBase)
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-68
        Cytogenetic map
        49F4-49F4
        Sequence location
        2R:13,163,959..13,168,032 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        49F4-49F4
        Limits computationally determined from genome sequence between P{lacW}Psck07834&P{lacW}Su(z)2k06344 and P{EP}EP873EP873
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location
        2-68
        (FlyBase, 2016)
        2-71.6
        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (11)
        Genomic Clones (10)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (6)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for identity of: GLaz CG4604
        (FlyBase, 1996-)
        Source for database merge of
        Additional comments
        Source for identity of GLaz CG4604 was sequence comparison ( date:000721 ).
        (FlyBase, 1996-)
        Other Comments
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 46 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Synonyms and Secondary IDs (8)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (50)