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Gene Dmel\FLASH

General Information
Symbol	Dmel\FLASH	Species	D. melanogaster
Name	FLASH ortholog	Annotation symbol	CG4616
Feature type	protein_coding_gene	FlyBase ID	FBgn0033806
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dFLASH
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	49F8-49F9	Sequence location	2R:9,094,250..9,097,974 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene FLASH ortholog is referred to in FlyBase by the symbol Dmel\FLASH (CG4616, FBgn0033806). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: histone mRNA 3'-end processing. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1627164_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG4616. Gene sequence location is 2R:9094250..9097974.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 49F8-49F9   Limits computationally determined from genome sequence between P{EP}CG33182EP728&P{lacW}Aats-valk14804 and P{EP}DpEP358
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\FLASH for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) FLASH-RA FBtr0087736  NM_137001   2830   844 FLASH-RB FBtr0087737  NM_206106   3486   844
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein FLASH-PA   FBpp0086849   94.7   844   5.86     NP_610845   AAF58410 FLASH-PB   FBpp0086850   94.7   844   5.86     NP_995828   AAS64862
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA438289   AE013599 AAF58410   AE013599 AAS64862   AI546472   AW942225   AW943959   AY069710 AAL39855   AY070537 AAL48008   BI170158   BI212518   BI367679   CO176915   CO190941   CO275107   CO341913   CZ467628   EC058126   EC060652   EC069582   EC201126   EC252746   ES694891
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K0L8 Q8SZS8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
histone locus body inferred from direct assay (Yang et al., 2009, White et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    21.175 Larval Midgut    5.3 Larval Hindgut    7.6 Larval Malpighian Tubules    10.7 Larval Fat Body    25 Larval Salivary Gland    11.9 Larval Trachea    10.875 Larval Carcass    10.5 Adult Head    8.4 Adult Eye    4.725 Adult Brain    10.4 Adult Thoracic-Abdominal Ganglion    11.9 Adult Crop    6.5 Adult Midgut    4.3 Adult Hindgut    6.2 Adult Malpighian Tubules    4.4 Adult Fat Body    12.5 Adult Salivary Gland    9.8 Adult Heart    3.825 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    12.6 Adult Ovary    25.4 Adult Testis    49 Adult Male Accessory Gland    11 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    8.4   NA  Eye    4.725   NA  Brain    10.4   21.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    6.5   5.3  Midgut    4.3   7.6  Hindgut    6.2   no informative data  Malpighian Tubules    4.4   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    9.8   NA  Heart    3.825   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    8.1   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    25.4   NA  Testis    49   NA  Male Accessory Gland    no informative data   no informative data  Carcass    7.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female  (29) ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female  29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    12 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    2 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    23 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    19 embryo 04-06hr    16 embryo 06-08hr    11 embryo 08-10hr    10 embryo 10-12hr    5 embryo 12-14hr    7 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    3 adult female 05day    9 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    9 embryonic 1182-4H    7 embryonic GM2    13 embryonic Kc167    20 embryonic S1    12 embryonic S3    13 leg disc CME L1    11 wing disc CME-W2    13 wing disc ML-DmD8    10 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    6 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    12 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033806 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4616-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 FLASHGD11373 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of FLASH Class Mutagen Stocks Known lesion FLASHc02024 piggyBac transposase 1 -- FLASHCB-6957-3 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of FLASH Class Mutagen Stocks Known lesion FLASHGD11373 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes FLASHKK103526 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes FLASHNIG.4616R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel8057 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.4616R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS3}FLASHCB-6957-3 NA PBac{PB}FLASHc02024 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
histone mRNA 3'-end processing inferred from mutant phenotype (Yang et al., 2009)
Cellular Component
CV term Evidence References
histone locus body inferred from direct assay (Yang et al., 2009, White et al., 2011)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
FLASH - CG6272 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) FLASH - Rcd5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions FLASH allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0033806
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6907VF Dipteran inclusive ortholog search EOG65TCW9 Insect inclusive ortholog search EOG6J3VC0 Arthropod inclusive ortholog search EOG65TB33 Metazoa inclusive ortholog search EOG6C5RBC
Orthologs in Drosophila Species (EOG6907VF)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\FLASH     Drosophila simulans   Dsim\GD10949 Y   Drosophila sechellia   Dsec\GM21450 Y   Drosophila erecta   Dere\GG20363 Y   Drosophila yakuba   Dyak\GE12523 Y   Drosophila ananassae   Dana\GF11946 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18304 Y   Drosophila persimilis   Dper\GL16802 Y   Drosophila willistoni   Dwil\GK17912 Y   Drosophila virilis   Dvir\GJ20866 Y   Drosophila mojavensis   Dmoj\GI21263 Y   Drosophila grimshawi   Dgri\GH25005 Y   Drosophila grimshawi   Dgri\GH17546
Orthologs in non-Drosophila Dipterans (EOG65TCW9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010677
Orthologs in non-Dipteran Insects (EOG6J3VC0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Pediculus humanus Human body louse  Phum\PHUM220690
Orthologs in non-Insect Arthropods (EOG65TB33)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_307765
Orthologs in non-Arthropod Metazoa (EOG6C5RBC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015063gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10949 Drosophila sechellia Dsec\GM21450 Drosophila erecta Dere\GG20363 Drosophila yakuba Dyak\GE12523 Drosophila ananassae Dana\GF11946 Drosophila pseudoobscura pseudoobscura Dpse\GA18304 Drosophila persimilis Dper\GL16802 Drosophila willistoni Dwil\GK17912 Drosophila virilis Dvir\GJ20866 Drosophila mojavensis Dmoj\GI21263 Drosophila grimshawi Dgri\GH25005
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Harvard	- PBac{PB}FLASHc02024
VDRC	v100082 P{KK103526}VIE-260B v21901 w1118; P{GD11373}v21901 v21902 w1118; P{GD11373}v21902
Genomic Clones ( 1 )
	BACR05M17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD12230 LD48056
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE11526 RE17623 RE53479
Other clones	14306 71790 214339.35 CK01.63B.D1 EK050210 EK051610 EK116455 EP13410
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0033806 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36455
Antibody Information
	polyclonal (Yang et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: FLASH CG4616 (Yang et al., 2009)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137001 NM_206106 RefSeq (Proteins) • NP_610845 NP_995828 DDBJ / EMBL / GenBank • AA438289 AAF58410 AAL39855 AAL48008 AAS64862 AI546472 AW942225 AW943959 AY069710 AY070537 CZ467628 Entrez Gene - A searchable database of RefSeq genes. • 36455 UniProtKB/TrEMBL • Q7K0L8 Q8SZS8
Other Crossreferences
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0033806 DRSC - Results from RNAi screens. • FBgn0033806 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0033806 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4616-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0033806 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36455 modMine - Data generated by the modENCODE project. • FBgn0033806
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG4616 (Yang et al., 2009, Keleman et al., 2009.8.5, Japanese National Institute of Genetics, 2012.5.21) dFLASH (Yang et al., 2009, Burch et al., 2011) FLASH (White et al., 2011, )
Name Synonym	FLASH ortholog
Secondary FlyBase IDs
References ( 17 )
Research paper	Burch et al., 2011, RNA 17(6): 1132--1147 Interaction between FLASH and Lsm11 is essential for histone pre-mRNA processing in vivo in Drosophila. [FBrf0213747] White et al., 2011, J. Cell Biol. 193(4): 677--694 Drosophila histone locus bodies form by hierarchical recruitment of components. [FBrf0213662] Yang et al., 2009, Mol. Cell 36(2): 267--278 FLASH, a proapoptotic protein involved in activation of caspase-8, is essential for 3' end processing of histone pre-mRNAs. [FBrf0209016]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Curated genome annotation	FamiliarityBreedsContempt, 1999.11, [Automatic annotations performed at the Celera Jamboreee.] [Automatic annotations performed at the Celera Jamboreee.] [FBrf0126705]