Gene Dmel\mars

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General Information

Symbol

Dmel\mars

Species

D. melanogaster

Name

mars

Annotation symbol

CG17064

Feature type

protein_coding_gene

FlyBase ID

FBgn0033845

Gene Model Status

Current

Stock availability

4 publicly available

Also Known As

HURP

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

50A13-50A14

Sequence location

2R:9,390,080..9,393,516 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene mars is referred to in FlyBase by the symbol Dmel\mars (CG17064, FBgn0033845). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein phosphatase 1 binding. There is experimental evidence that it is involved in the biological process: spindle stabilization; kinetochore assembly; centrosome localization; chromosome segregation; regulation of mitotic cell cycle; mitotic spindle organization; M phase of mitotic cell cycle. 17 alleles are reported. The phenotypes of these alleles are annotated with: eye disc; eye; egg; meiotic spindle; spindle; embryo. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Guanylate-kinase-associated protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1637994_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of mars. Gene sequence location is 2R:9390080..9393516.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50A13-50A14

Limits computationally determined from genome sequence between P{lacW}drk^k13809&P{EP}mip120^EP606 and P{lacW}l(2)03105^k16702&P{lacW}fl(2)d^k16105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\mars for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

mars-RA

FBtr0087668

NM_137034

3026

921

mars-RB

FBtr0301387

NM_001169671

3323

921

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mars-PA

FBpp0086788

101.9

921

10.69

NP_610878

AAF58367

mars-PB

FBpp0290601

101.9

921

10.69

NP_001163142

ACZ94415

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Guanylate-kinase-associated protein (IPR005026)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7K3L1

UniProtKB/TrEMBL

E1JH57

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male & spermatogenesis

primary spermatocyte & secondary spermatocyte

(Bennett and Alphey, 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 3 -- 4

organism | ubiquitous

(Bennett and Alphey, 2004)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

yolk sac

(Bennett and Alphey, 2004)

Comment:expression excluded from epithelium and pole cells

embryonic stage 6 -- 11

germ band

(Bennett and Alphey, 2004)

embryonic foregut & embryonic hindgut

(Bennett and Alphey, 2004)

embryonic procephalic segment

(Bennett and Alphey, 2004)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

central brain primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

visual primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

dorsal head epidermis primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

lateral head epidermis primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

ventral head epidermis primordium

(Fisher et al., 2012)

Comment:reported as procephalic ectoderm primordium

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

dorsal sensory complex primordium

(Fisher et al., 2012)

embryonic ganglion mother cell

(Fisher et al., 2012)

embryonic neuroblast of ventral nerve cord primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

procephalic neuroblast

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

ventral sensory complex anlage

(Fisher et al., 2012)

embryonic stage 12

procephalic neurogenic region

(Bennett and Alphey, 2004)

ventral neurectoderm

(Bennett and Alphey, 2004)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain surface glia

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

lateral cord surface glial cell

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic stage 13 -- 17

presumptive embryonic/larval central nervous system

(Bennett and Alphey, 2004)

embryonic brain

(Bennett and Alphey, 2004)

third instar larval stage

embryonic/larval optic lobe

(Bennett and Alphey, 2004)

ventral nerve cord

(Bennett and Alphey, 2004)

Comment:weakly expressed

larval inner optic anlage

(Bennett and Alphey, 2004)

Comment:strongly expressed in proliferative center

dorsal proliferation zone & inner proliferation zone

(Bennett and Alphey, 2004)

eye disc posterior to the morphogenetic furrow

(Bennett and Alphey, 2004)

mesothoracic tergum primordium

(Bennett and Alphey, 2004)

ventral thoracic disc

(Bennett and Alphey, 2004)

adult stage

germline cell

(Bennett and Alphey, 2004)

adult stage | male

testis

(Bennett and Alphey, 2004)

Comment:not observed in somatic cyst cells or spermatogonial mitotically proliferating cells at apical tip of testis

adult stage | female & oogenesis stage S10

ovariole

(Bennett and Alphey, 2004)

Comment:not observed in follicle cells

oocyte

(Bennett and Alphey, 2004)

oogenesis stage S10 & adult stage | female

nurse cell

(Bennett and Alphey, 2004)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Bennett and Alphey, 2004)

Comment:expressed 0-24 hr AEL

third instar larval stage

organism

(Bennett and Alphey, 2004)

P-stage

organism

(Bennett and Alphey, 2004)

adult stage | male

testis

(Bennett and Alphey, 2004)

adult stage | female

ovary

(Bennett and Alphey, 2004)

Additional Descriptive Data

Maternally expressed mars transcript is ubiquitously distributed in syncytial blastoderm embryos. In cellularized blastoderm embryos, transcript is localized to the yolk, and excluded from epithelial cells and pole cells. From the onset of gastrulation until embryonic stage 11, expression is observed in the procephalic segment, the germ band, the foregut and the midgut. After embryonic stage 11, expression is increasingly restricted to the ventral and procephalic neurogenic region. After germ band retraction, mars transcript is expressed only in the central nervous system and brain. In third instar larvae, intense expression is observed in the dorsal and inner proliferation zones of the larval optic lobes; expression in the rest of the optic lobe, and in the ventral nerve cord, is diffuse. Third instar larval expression is also observed in the presumptive notum of the wing disc; in a band behind the the morphogenetic furrow of the eye disc; and diffusely in leg discs. Adult expression is confined to the ovaries and testes. In the testis, mars transcript is expressed in primary and secondary spermatocytes, but not in somatic cyst cells, or in spermatogonial mitotically proliferating cells at apical tip of testis. Ovariole expression is observed in oocytes and nurse cells, but not in follicle cells.

(Bennett and Alphey, 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

epitope tag

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

epithelial cell of wing disc

• plasma membrane

(Bennett and Alphey, 2004)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

organism

(Bennett and Alphey, 2004)

adult stage | male

testis

(Bennett and Alphey, 2004)

adult stage | female

ovary

(Bennett and Alphey, 2004)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Yang et al., 2005, Yang and Fan, 2008)

colocalizes_with
kinetochore microtubule

inferred from direct assay

(Yang and Fan, 2008)

nucleoplasm

inferred from direct assay

(Yang et al., 2005)

nucleus

inferred from direct assay

(Zhang et al., 2009)

spindle

inferred from direct assay

(Yang and Fan, 2008)

spindle microtubule

inferred from direct assay

(Zhang et al., 2009)

spindle pole

inferred from direct assay

(Zhang et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		147.2
Larval Midgut		21.6
Larval Hindgut		18.6
Larval Malpighian Tubules		12.4
Larval Fat Body		53.2
Larval Salivary Gland		10.9
Larval Trachea		35.475
Larval Carcass		22.35
Adult Head		7.1
Adult Eye		3.95
Adult Brain		17.2
Adult Thoracic-Abdominal Ganglion		17.9
Adult Crop		18
Adult Midgut		14.2
Adult Hindgut		10.9
Adult Malpighian Tubules		10.7
Adult Fat Body		10.5
Adult Salivary Gland		14.7
Adult Heart		7.025
Adult VirginFemale Spermatheca		16.4
Adult InseminatedFemale Spermatheca		11.7
Adult Ovary		223.3
Adult Testis		150
Adult Male Accessory Gland		24.4
Adult Carcass		22.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	7.1
NA	Eye	3.95
NA	Brain	no informative data
147.2	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	no informative data
NA	Crop	no informative data
no informative data	Midgut	no informative data
no informative data	Hindgut	no informative data
no informative data	Malpighian Tubules	no informative data
no informative data	Fat Body	no informative data
no informative data	Salivary Gland	no informative data
NA	Heart	7.025
35.475	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	223.3
NA	Testis	150
NA	Male Accessory Gland	no informative data
no informative data	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(56)
central nervous system, larvae L3		(30)
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		(78)
ovary, mated 4-day female		(74)
testis, mated 4-day male		(84)
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(56)
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		(78)
ovary, mated 4-day female		(74)
testis, mated 4-day male		(84)
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		56
central nervous system, larvae L3		30
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		2
digestive system, 4-day adult		3
digestive system, 20-day adult		2
fat body, larvae L3 wandering		2
fat body, white prepupae		11
fat body, pupae P8		7
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		78
ovary, mated 4-day female		74
testis, mated 4-day male		84
accessory gland, mated 4-day male		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(92)
embryo 02-04hr		(78)
embryo 04-06hr		(72)
embryo 06-08hr		(80)
embryo 08-10hr		(56)
embryo 10-12hr		(30)
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		(35)
adult female 30day		(39)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(92)
embryo 02-04hr		(78)
embryo 04-06hr		(72)
embryo 06-08hr		(80)
embryo 08-10hr		(56)
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		(35)
adult female 30day		(39)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		92
embryo 02-04hr		78
embryo 04-06hr		72
embryo 06-08hr		80
embryo 08-10hr		56
embryo 10-12hr		30
embryo 12-14hr		19
embryo 14-16hr		22
embryo 16-18hr		16
embryo 18-20hr		8
embryo 20-22hr		25
embryo 22-24hr		20
larva L1		1
larva L2		2
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		12
larva L3 puffstage 7-9		12
white prepupae new		10
white prepupae 12hr		23
white prepupae 24hr		26
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		13
adult male 05day		23
adult male 30day		20
adult female 01day		15
adult female 05day		35
adult female 30day		39
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(52)
Schneider line 2 Sg4		20
embryonic 1182-4H		(34)
embryonic GM2		20
embryonic Kc167		16
embryonic S1		(40)
embryonic S3		(33)
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		(27)
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(46)
wing disc ML-DmD21		(37)
wing disc ML-DmD32		(43)
haltere disc ML-DmD17-c3		(35)
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		(28)
CNS ML-DmBG1-c1		(43)
CNS ML-DmBG2-c2		(27)
tumorous blood cells mbn2		17
ovary fGS/OSS		(53)
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(52)
Schneider line 2 Sg4		20
embryonic 1182-4H		(34)
embryonic GM2		20
embryonic Kc167		16
embryonic S1		(40)
embryonic S3		(33)
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(46)
wing disc ML-DmD21		(37)
wing disc ML-DmD32		(43)
haltere disc ML-DmD17-c3		(35)
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		(43)
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		(53)
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		52
Schneider line 2 Sg4		20
embryonic 1182-4H		34
embryonic GM2		20
embryonic Kc167		16
embryonic S1		40
embryonic S3		33
leg disc CME L1		24
wing disc CME-W2		16
wing disc ML-DmD8		27
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		46
wing disc ML-DmD21		37
wing disc ML-DmD32		43
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		24
antennal disc ML-DmD20-c5		22
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		43
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		17
ovary fGS/OSS		53
ovary OSC		18
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		(59)
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		(27)
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		(54)
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(53)
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		(41)
Paraquat 10 mM 48 hrs, 4-day adult		(31)
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		(59)
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		(54)
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(53)
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		(41)
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		16
heat shock, 4-day adult		22
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		22
Cadmium 100 mM 48 hrs, 4-day adult		59
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		54
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		23
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD21642

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033845

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG17064-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for mars

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

mars¹⁰²

lethal, with Scer\GAL4^ap-md544

mars^{Scer\UAS.T:Zzzz\FLAG}

lethal, with Scer\GAL4^dpp.blk1

mars^{Scer\UAS.T:Zzzz\FLAG}

lethal, with Scer\GAL4^ey.PH

mars^{Scer\UAS.T:Zzzz\FLAG}

lethal, with Scer\GAL4^ptc-559.1

mars^{Scer\UAS.T:Zzzz\FLAG}

lethal | embryonic stage

mars¹

viable

viable, with Scer\GAL4^pnr-MD237

mars^GD9277

viable (with Df(2R)CX1)

mars⁹¹

viable | temperature conditional

mars^EP2477

Sterility

Allele

female sterile

fertile

fertile (with Df(2R)CX1)

mars⁹¹

grandchildless | partially

mars⁹¹

grandchildless | partially (with Df(2R)CX1)

mars⁹¹

Other Phenotypes

Allele

hatching behavior defective

mars¹

increased cell death, with Scer\GAL4^GMR.PF

mars^{Scer\UAS.T:Zzzz\FLAG}

mitotic cell cycle defective

mars^EP2477

mitotic cell cycle defective, with Scer\GAL4^GMR.PF

mitotic cell cycle defective, with Scer\GAL4^tub.PU

mars^{dsRNA.Scer\UAS.WIZ}

mitotic cell cycle defective | conditional

mars⁹¹

visible, with Scer\GAL4^GMR.PF

Phenotype manifest in

Allele

centrosome | conditional | embryonic stage 4

mars⁹¹

egg

mars^EP2477

egg (with mars¹)

mars^EP2477

egg (with mars^EP2477)

mars¹

embryo

mars^EP2477

embryo (with mars¹)

mars^EP2477

embryo (with mars^EP2477)

mars¹

eye, with Scer\GAL4^GMR.PF

eye disc, with Scer\GAL4^GMR.PF

mars^{Scer\UAS.T:Zzzz\FLAG}

eye disc, with Scer\GAL4^tub.PU

mars^{dsRNA.Scer\UAS.WIZ}

meiotic spindle

mars¹

nucleus | conditional | embryonic stage 4

mars⁹¹

spindle

spindle | conditional | embryonic stage 4

mars⁹¹

spindle pole | conditional | embryonic stage 4

mars⁹¹

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of mars	Class	Mutagen	Stocks	Known lesion
mars^f07689		piggyBac transposase	2	--
mars¹⁰²		Delta2-3	0	Yes
mars¹		P-element activity	0	Yes
mars⁹¹	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
mars^EP2477		P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mars	Mutagen	Stocks	Known lesion
mars^GD9277	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mars^HMS00878	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mars^{dC.Scer\UAS.T:Zzzz\FLAG}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes
mars^{dM.Scer\UAS.T:Zzzz\FLAG}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes
mars^{dN.Scer\UAS.T:Zzzz\FLAG}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes
mars^{dsRNA.Scer\UAS.WIZ}	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
mars^{F839A.Scer\UAS.P\T}	in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis	0	Yes
mars^KK100727	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
mars^{Scer\UAS.P\T.cTa}	in vitro construct - regulatory fusion	0	Yes
mars^{Scer\UAS.P\T.T:Avic\GFP-EGFP}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes
mars^{Scer\UAS.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	--
mars^{Scer\UAS.T:Zzzz\FLAG}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)mars¹⁰²

(Zhang et al., 2009)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}drk^EP2477

miscellaneous insertions

P{SUPor-P}drk^KG03077

PBac{WH}mars^f07689

Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )

Terms Based on Experimental Evidence ( 15 terms )

Molecular Function

CV term

Evidence

References

protein phosphatase 1 binding

inferred from physical interaction with flw

(Bennett et al., 2006)

inferred from physical interaction with Pp1-13C

(Bennett et al., 2006)

inferred from physical interaction with Pp1-87B

(Bennett et al., 2006)

inferred from physical interaction with Pp1α-96A

(Bennett et al., 2006)

Biological Process

CV term

Evidence

References

centrosome localization

inferred from mutant phenotype

(Zhang et al., 2009)

chromosome segregation

inferred from genetic interaction with tlk

(Li et al., 2009)

kinetochore assembly

inferred from mutant phenotype

(Yang and Fan, 2008)

mitotic spindle organization

inferred from mutant phenotype

(Zhang et al., 2009)

M phase of mitotic cell cycle

inferred from mutant phenotype

(Li et al., 2009)

regulation of mitotic cell cycle

inferred from mutant phenotype

(Yang et al., 2005, Yang and Fan, 2008)

spindle stabilization

inferred from direct assay

(Tan et al., 2008)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Yang et al., 2005, Yang and Fan, 2008)

colocalizes_with
kinetochore microtubule

inferred from direct assay

(Yang and Fan, 2008)

nucleoplasm

inferred from direct assay

(Yang et al., 2005)

nucleus

inferred from direct assay

(Zhang et al., 2009)

spindle

inferred from direct assay

(Yang and Fan, 2008)

spindle microtubule

inferred from direct assay

(Zhang et al., 2009)

spindle pole

inferred from direct assay

(Zhang et al., 2009)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

cell-cell signaling

inferred from electronic annotation with InterPro:IPR005026

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

mars - CG14141