Gene Dmel\CG8494

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General Information

Symbol

Dmel\CG8494

Species

D. melanogaster

Name

Annotation symbol

CG8494

Feature type

protein_coding_gene

FlyBase ID

FBgn0033916

Gene Model Status

Current

Stock availability

6 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

50E4-50E5

Sequence location

2R:10,127,437..10,131,160 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG8494 (FBgn0033916). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: ubiquitin thiolesterase activity. There is experimental evidence that it is involved in the biological process: nervous system development; protein ubiquitination. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2; Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2, conserved site; Peptidase C19, ubiquitin-specific peptidase, DUSP domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult heart, adult fat body, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1626786_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8494. Gene sequence location is 2R:10127437..10131160.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50E4-50E5

Limits computationally determined from genome sequence between P{lacW}l(2)s3475^s3475&P{lacW}Hsc70-5^k04907 and P{PZ}Tfb1⁰⁶⁹⁴⁹&P{PZ}l(2)03563⁰³⁵⁶³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG8494 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG8494-RA

FBtr0087535

NM_137099

3423

975

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG8494-PA

FBpp0086664

109.7

975

5.34

NP_610943

AAF58273

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394)

Peptidase C19, ubiquitin-specific peptidase, DUSP domain (IPR006615)

Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2, conserved site (IPR018200)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult heart, adult fat body, adult ovary, adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		92.3
Larval Midgut		40.4
Larval Hindgut		42.5
Larval Malpighian Tubules		52.8
Larval Fat Body		42.7
Larval Salivary Gland		44.3
Larval Trachea		36.975
Larval Carcass		43.325
Adult Head		46.1
Adult Eye		59.6
Adult Brain		89.4
Adult Thoracic-Abdominal Ganglion		78.1
Adult Crop		60.1
Adult Midgut		46.8
Adult Hindgut		48.1
Adult Malpighian Tubules		62.7
Adult Fat Body		118.2
Adult Salivary Gland		94.6
Adult Heart		114
Adult VirginFemale Spermatheca		40
Adult InseminatedFemale Spermatheca		49.9
Adult Ovary		200.7
Adult Testis		352.7
Adult Male Accessory Gland		53.5
Adult Carcass		86.7
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	46.1
NA	Eye	59.6
NA	Brain	89.4
92.3	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	78.1
NA	Crop	60.1
40.4	Midgut	46.8
42.5	Hindgut	48.1
52.8	Malpighian Tubules	62.7
42.7	Fat Body	118.2
44.3	Salivary Gland	94.6
NA	Heart	114
36.975	Trachea	NA
NA	VirginFemale Spermatheca	40
NA	InseminatedFemale Spermatheca	49.9
NA	Ovary	200.7
NA	Testis	352.7
NA	Male Accessory Gland	53.5
43.325	Carcass	86.7

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(37)
ovary, mated 4-day female		(32)
testis, mated 4-day male		(55)
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(37)
ovary, mated 4-day female		32
testis, mated 4-day male		(55)
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		5
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		5
head, mated 4-day female		6
head, mated 20-day female		4
head, mated 1-day male		8
head, mated 4-day male		9
head, mated 20-day male		10
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		9
fat body, pupae P8		9
carcass, larvae L3 wandering		7
carcass, 1-day adult		4
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		37
ovary, mated 4-day female		32
testis, mated 4-day male		55
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(44)
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		(27)
adult male 05day		(35)
adult male 30day		(32)
adult female 01day		15
adult female 05day		(27)
adult female 30day		(28)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(44)
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		(35)
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		44
embryo 02-04hr		12
embryo 04-06hr		16
embryo 06-08hr		16
embryo 08-10hr		14
embryo 10-12hr		10
embryo 12-14hr		9
embryo 14-16hr		7
embryo 16-18hr		6
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		5
larva L1		6
larva L2		6
larva L3 12hr old		5
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		12
white prepupae new		12
white prepupae 12hr		14
white prepupae 24hr		20
pupae 2d postWPP		17
pupae 3d postWPP		16
pupae 4d postWPP		14
adult male 01day		27
adult male 05day		35
adult male 30day		32
adult female 01day		15
adult female 05day		27
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		8
embryonic 1182-4H		8
embryonic GM2		17
embryonic Kc167		8
embryonic S1		11
embryonic S3		13
leg disc CME L1		10
wing disc CME-W2		11
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		14
wing disc ML-DmD21		4
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		15
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		(28)
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		(27)
Paraquat 10 mM 48 hrs, 4-day adult		(27)
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		6
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		14
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		21
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		27
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD43649

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0033916

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8494-RA;CG31050-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Other Phenotypes

Allele

locomotor behavior defective | adult stage, with Scer\GAL4^elav.PU

CG8494^GD13944

short lived, with Scer\GAL4^sqh.PW

CG8494^GD13944

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG8494	Class	Mutagen	Stocks	Known lesion
CG8494^e04168		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG8494	Mutagen	Stocks	Known lesion
CG8494^GD13944	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG8494^GD8681	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG8494^KK104810	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)Exel7131

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD8681}

P{GD13944}

P{KK104810}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}CG8494^e04168

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

nervous system development

inferred from mutant phenotype

(Koizumi et al., 2007)

protein ubiquitination

inferred from mutant phenotype

(Tsou et al., 2012)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

ubiquitin thiolesterase activity

inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR006615, InterPro:IPR018200

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG8494 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

62330

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0033916

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

36580

Orthologs

OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6VDQHZ

Dipteran inclusive ortholog search

EOG6PRSZQ

Insect inclusive ortholog search

EOG6547TK

Arthropod inclusive ortholog search

EOG60GB5S

Metazoa inclusive ortholog search

EOG67HJGR

Orthologs in Drosophila Species (EOG6VDQHZ)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25084

Drosophila persimilis

Dper\GL10121

Drosophila willistoni

Dwil\GK20778

Drosophila virilis

Dvir\GJ20067

Drosophila mojavensis

Dmoj\GI19092

Drosophila grimshawi

Dgri\GH19806

Orthologs in non-Drosophila Dipterans (EOG6PRSZQ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009702

Anopheles gambiae

Malaria mosquito

Agam\AGAP003821

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016898

Orthologs in non-Dipteran Insects (EOG6547TK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB40540

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012741

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH25002

Atta cephalotes

Leafcutter ant

Acep\ACEP25975

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO18951

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL14494

Linepithema humile

Argentine ant

Lhum\LH18772

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB12569

Solenopsis invicta

Red fire ant

Sinv\SINV17433

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007353

Bombyx mori

Silkmoth

Bmor\BGIBMGA004500

Pediculus humanus

Human body louse

Phum\PHUM503840

Tribolium castaneum

Red flour beetle

Tcas\TC030699

Orthologs in non-Insect Arthropods (EOG60GB5S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_309166

Daphnia pulex

Water flea

Dpux\COI

Ixodes scapularis

Deer tick

Isca\ISCW005337

Orthologs in non-Arthropod Metazoa (EOG67HJGR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\usp-33

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_004323gn

Danio rerio

Zebrafish

Drer\usp20

Danio rerio

Zebrafish

Drer\usp33

Xenopus tropicalis

Western clawed frog

Xtro\usp20

Xenopus tropicalis

Western clawed frog

Xtro\usp33

Gallus gallus

Domestic chicken

Ggal\USP33

Gallus gallus

Domestic chicken

Ggal\USP20

Mus musculus

House mouse

Mmus\Usp20

Mus musculus

House mouse

Mmus\ENSMUSG00000025437

Rattus norvegicus

Norway rat

Rnor\Usp20

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000011294

Homo sapiens

Human

Hsap\ENSG00000077254

Homo sapiens

Human

Hsap\USP20

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\ENSG00000077254

Hsap\USP20

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25084

Drosophila persimilis

Dper\GL10121

Drosophila willistoni

Dwil\GK20778

Drosophila virilis

Dvir\GJ20067

Drosophila mojavensis

Dmoj\GI19092

Drosophila grimshawi

Dgri\GH19806

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

18231

w¹¹¹⁸; PBac{RB}CG8494^e04168/CyO

Harvard

PBac{RB}CG8494^e04168

VDRC

v23934

w¹¹¹⁸; P{GD8681}v23934

v28910

w¹¹¹⁸; P{GD13944}v28910

v42609

w¹¹¹⁸; P{GD13944}v42609

v23935

w¹¹¹⁸; P{GD8681}v23935/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 40 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones