FlyBase Gene Report: Dmel\Cyp6a20

General Information

Symbol

Dmel\Cyp6a20

Species

D. melanogaster

Name

Cytochrome P450 6a20

Annotation Symbol

CG10245

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033980

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Cyp6a20 (Cyp6a20) encodes a protein that may function to degrade aggression-promoting pheromones based on indirect observations such as its expression pattern and the increased aggressive behavior shown in Cyp6a20 mutants. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

6a20

Key Links

Genomic Location

Cytogenetic map

51D1-51D1

Sequence location

2R:14,882,118..14,884,046 [+]

Recombination map

2-73

RefSeq locus

NT_033778 REGION:14882118..14884046

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables heme binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables iron ion binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables monooxygenase activity

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR036396

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR017972

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in aggressive behavior

inferred from mutant phenotype

(Wang et al., 2008)

involved_in defense response to Gram-negative bacterium

inferred from mutant phenotype

(Berkey et al., 2009)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN

Protein Family (UniProt)

Belongs to the cytochrome P450 family. (Q9V773)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN -: Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The CYP3 clan includes D. melanogaster CYP6, 9, 28, 308-310 and 317 family genes. Few CYP3 enzymes have been characterized, though many have been shown to metabolize xenobiotics and plant natural compounds. (Adapted from FBrf0248421.)

Protein Function (UniProtKB)

May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.

(UniProt, Q9V773)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cyp6a20 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V773)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Mutation in sequenced strain: frameshift (-1).

A single nucleotide deletion introduces a frame shift in the sequence.(see FBrf0136021)

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cyp6a20-RB

FBtr0300127

NM_137158

1801

501

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Cyp6a20-PB

FBpp0289404

57.7

501

8.23

NP_611002

AAF58187

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V773

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyp6a20 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cyp6a20_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT21-0

(Xu et al., 2011)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cyp6a20 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cyp6a20

View exonic expression by tissue for Dmel\Cyp6a20

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033980

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36664/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033980

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033980

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033980

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10245

Flygut - An atlas of the Drosophila adult midgut

FBgn0033980

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cyp6a20

Transgenic constructs containing regulatory region of Cyp6a20

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cyp6a20^GD1184

viable, with Scer\GAL4^Mef2.PR

Cyp6a20^GD1184

viable, with Scer\GAL4^pnr-MD237

Cyp6a20^GD1184

viable, with Scer\GAL4^tin.CΔ4

Cyp6a20^GD1184

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CYP3A4

5 of 15

Yes

Hsap\CYP3A5

5 of 15

Yes

Hsap\CYP3A7-CYP3A51P

5 of 15

Yes

Hsap\CYP3A43

5 of 15

Yes

Hsap\TBXAS1

5 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cyp3a11

4 of 15

Yes

Mmus\Cyp3a16

4 of 15

Yes

Mmus\Cyp3a25

4 of 15

Yes

Mmus\Cyp3a41a

4 of 15

Yes

Mmus\Cyp3a44

4 of 15

Yes

Mmus\Cyp3a57

4 of 15

Yes

Mmus\Cyp3a59

4 of 15

Yes

Mmus\Tbxas1

4 of 15

Yes

Mmus\Cyp3a13

3 of 15

Mmus\Cyp3a41b

1 of 15

Rattus norvegicus (Norway rat) (9)

Rnor\Cyp3a9

5 of 13

Yes

Rnor\Cyp3a18

5 of 13

Yes

Rnor\Tbxas1

5 of 13

Yes

4 of 13

4 of 13

3 of 13

2 of 13

1 of 13

Yes

Rnor\Cyp3a73

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (8)

Xtro\tbxas1

4 of 12

Yes

Xtro\cyp3a4

3 of 12

Yes

Xtro\cyp3a5

3 of 12

Yes

Xtro\LOC100494419

3 of 12

Yes

Xtro\LOC100487054

1 of 12

Xtro\LOC100487665

1 of 12

Yes

Xtro\LOC100487846

1 of 12

Xtro\LOC105945483

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\cyp3c2

6 of 15

Yes

Drer\cyp3c3

6 of 15

Yes

5 of 15

3 of 15

3 of 15

3 of 15

Caenorhabditis elegans (Nematode, roundworm) (21)

Cele\cyp-13A1

5 of 15

Yes

Cele\cyp-13A2

5 of 15

Yes

Cele\cyp-13A3

5 of 15

Yes

Cele\cyp-13A4

5 of 15

Yes

Cele\cyp-13A5

5 of 15

Yes

Cele\cyp-13A6

5 of 15

Yes

Cele\cyp-13A7

5 of 15

Yes

Cele\cyp-13A8

5 of 15

Yes

Cele\cyp-13A10

5 of 15

Yes

Cele\cyp-13A11

5 of 15

Yes

Cele\cyp-13A12

5 of 15

Yes

Cele\cyp-13B1

5 of 15

Yes

Cele\cyp-13B2

5 of 15

Yes

Cele\cyp-25A1

5 of 15

Yes

Cele\cyp-25A2

5 of 15

Yes

Cele\cyp-25A3

5 of 15

Yes

Cele\cyp-25A4

5 of 15

Yes

4 of 15

3 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (48)

Atha\CYP711A1

3 of 9

Yes

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ERG11

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\erg5

2 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (36)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

CYP3A5; cytochrome P450 family 3 subfamily A member 5

model of essential hypertension

IEA

(FlyBase, 2019-)

TBXAS1; thromboxane A synthase 1

model of Ghosal hematodiaphyseal syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CYP3A4; cytochrome P450 family 3 subfamily A member 4

5 of 15

124010

VITAMIN D-DEPENDENT RICKETS, TYPE 3; VDDR3

CYP3A5; cytochrome P450 family 3 subfamily A member 5

5 of 15

605325

HYPERTENSION, ESSENTIAL

essential hypertension

CYP3A7-CYP3A51P; CYP3A7-CYP3A51P readthrough

5 of 15

CYP3A43; cytochrome P450 family 3 subfamily A member 43

5 of 15

606534

TBXAS1; thromboxane A synthase 1

5 of 15

274180

GHOSAL HEMATODIAPHYSEAL DYSPLASIA; GHDD

Ghosal hematodiaphyseal syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62406

DroID - A comprehensive database of gene and protein interactions.

FBgn0033980

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-73

Cytogenetic map

51D1-51D1

Sequence location

2R:14,882,118..14,884,046 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51D1-51D1

Limits computationally determined from genome sequence between P{lacW}Rpn6^k00103 and P{PZ}chn⁰²⁰⁶⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51C-51C

(Dunkov, 2000.6.29)

Experimentally Determined Recombination Data

Location

2-73

(FlyBase, 2016)

2-76.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

85145

w¹¹¹⁸; PBac{RB}Cyp6a20^e02611

67314

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06418}attP40

VDRC

v3313

w¹¹¹⁸; P{GD1184}v3313

v3314

w¹¹¹⁸; P{GD1184}v3314

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (23)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD04231

Other clones

FI09616

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033980

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033980

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36664

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36664

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V773

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V773

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V773

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033980

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033980

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36664/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033980

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cyp6a20_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033980

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033980

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10245

Flygut - An atlas of the Drosophila adult midgut

FBgn0033980

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36664

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10245

MARRVEL_MODEL - MARRVEL (model organism gene)

36664

modMine - A data warehouse for the modENCODE project

FBgn0033980

Linkouts

BioGRID - A database of protein and genetic interactions.

62406

DroID - A comprehensive database of gene and protein interactions.

FBgn0033980

DRSC - Results frm RNAi screens

FBgn0033980

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033980

FlyMine - An integrated database for Drosophila genomics

FBgn0033980

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

6a20

(Kasai and Tomita, 2003, Nelson, 2001.2.5)

CG10245

(Ruppert et al., 2017, Palu and Thummel, 2016, Mosallanejad et al., 2010, Perkins et al., 2009, Rival et al., 2009, Lundgren et al., 2005)

CYP6a20

(Coelho et al., 2015)

agg

(Good et al., 2014)

p450

(Lundgren et al., 2005)

Name Synonyms

Cyp6a20

Cytochrome P450 6a20

Secondary FlyBase IDs

Datasets (6)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (6)

Result

Result Type

Title

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections