FlyBase Gene Report: Dmel\unc-5

General Information

Symbol

Dmel\unc-5

Species

D. melanogaster

Name

unc-5

Annotation Symbol

CG8166

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034013

Gene Model Status

Stock Availability

10 publicly available

Gene Snapshot

unc-5 (unc-5) encodes a netrin receptor involved in motor axon guidance and glial migration as well as netrin-mediated repulsion during salivary gland migration. [Date last reviewed: 2019-03-21]

Other Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

unc-5-like, CT20824, Unc5d

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:15,340,472..15,368,300 [+]

Recombination map

2-74

RefSeq locus

NT_033778 REGION:15340472..15368300

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

netrin receptor activity

inferred from electronic annotation with InterPro:IPR037936

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

netrin receptor activity involved in chemorepulsion

inferred from sequence or structural similarity

(Keleman and Dickson, 2001)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

inferred from mutant phenotype

(Brankatschk and Dickson, 2006, Keleman and Dickson, 2001)

inferred from genetic interaction with FLYBASE:NetB; FB:FBgn0015774

(Brankatschk and Dickson, 2006, Keleman and Dickson, 2001)

inferred from genetic interaction with FLYBASE:NetA; FB:FBgn0015773

(Brankatschk and Dickson, 2006)

glial cell migration

inferred from mutant phenotype

(von Hilchen et al., 2010, Freeman et al., 2003)

motor neuron axon guidance

inferred from mutant phenotype

(Zarin et al., 2014, Labrador et al., 2005)

positive regulation of cell migration

inferred from mutant phenotype

(Raza and Jacobs, 2016)

salivary gland boundary specification

inferred from mutant phenotype

(Kolesnikov and Beckendorf, 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

motor neuron axon guidance

inferred from biological aspect of ancestor with PANTHER:PTN002650052

(assigned by GO_Central )

(Gaudet et al., 2011)

netrin-activated signaling pathway

inferred from electronic annotation with InterPro:IPR037936

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

integral component of membrane

inferred from electronic annotation with InterPro:IPR037936

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Protein Family (UniProt)

Belongs to the unc-5 family. (Q95TU8)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Receptor for netrin required for motor axon guidance. Mediates both short- and long-range axon motor repulsion in the developing nervous system upon ligand binding. Also involved in glial migration. While short-range repulsion requires both fra and unc-5, long-range repulsion only requires unc-5.

(UniProt, Q95TU8)

Summary (Interactive Fly)

multiple domain protein that functions as a repulsive netrin receptor - essential for heart lumen formation

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the GBrowse view of Dmel\unc-5 or the JBrowse view of Dmel\unc-5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0087382

5033

1072

FBtr0339998

5235

1072

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0086514

116.4

1072

6.09

FBpp0309017

116.4

1072

6.09

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1072 aa isoforms: unc-5-PA, unc-5-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Post Translational Modification

Phosphorylated on different cytoplasmic tyrosine residues.

(UniProt, Q95TU8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 5

mesoderm anlage

(Keleman and Dickson, 2001)

embryonic stage 6 -- 14

(Keleman and Dickson, 2001)

embryonic stage 13 -- 14

dorsal vessel primordium

(Keleman and Dickson, 2001)

embryonic stage 13 -- 17

presumptive embryonic/larval central nervous system | restricted

(Keleman and Dickson, 2001)

embryonic stage 14 -- 17

exit glial cell

(Keleman and Dickson, 2001)

peripheral glial cell

(Keleman and Dickson, 2001)

embryonic stage 15

(Zarin et al., 2014)

RP2 motor neuron

(Zarin et al., 2014)

embryonic stage 13 -- 16

peripheral glial cell

(von Hilchen et al., 2010)

embryonic stage 14

embryonic/larval cardial cell

(Albrecht et al., 2011)

embryonic/larval pericardial cell

(Albrecht et al., 2011)

embryonic/larval dorsal vessel

(Albrecht et al., 2011)

Additional Descriptive Data

unc-5 trancripts are first detecteabel in the heart primordium at embryonic stage 14. They become more prominent by the end of embryogenesis. unc-5 ia expressed in all 104 cardiomyocytes of the heart. It is also expressed in pericardial cells.

(Albrecht et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

segmental nerve

(Keleman and Dickson, 2001)

embryonic stage 15 -- 16

embryonic/larval cardial cell

(Albrecht et al., 2011)

embryonic stage 16

embryonic/larval dorsal vessel

(Albrecht et al., 2011)

Additional Descriptive Data

At embryonic stage 15 before the cardiomyocytes from the bilateral primordia meet at the dorsal midline, unc-5 is localized to these cells in a polarized fashion. Strong signal is observed on the prospective lumen forming side and a slightly weaker signal is seen at the opposite membrane region. Essentially no proteinis detected on the lateral sides of cardiomyocytes.

(Albrecht et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\unc-5 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\unc-5

View exonic expression by tissue for Dmel\unc-5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034013

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8166-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8166

Flygut - An atlas of the Drosophila adult midgut

FBgn0034013

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of unc-5

Transgenic constructs containing regulatory region of unc-5

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^scrt-11-6

unc-5^GS15081

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

unc-5^GD3510

unc-5^MI05371

viable, with Scer\GAL4^Mef2.PR

unc-5^GD3510

viable, with Scer\GAL4^pnr-MD237

unc-5^GD3510

viable, with Scer\GAL4^tin.CΔ4

unc-5^GD3510

Other Phenotypes

Allele

cell migration defective | dominant | embryonic stage

unc-5⁸

locomotor rhythm defective, with Scer\GAL4^5-40

unc-5^UAS.cKa

neuroanatomy defective

neuroanatomy defective, with Scer\GAL4^5-40

unc-5^UAS.cKa

neuroanatomy defective, with Scer\GAL4^ap-md544

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

neuroanatomy defective, with Scer\GAL4^eg-Mz360

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

neuroanatomy defective, with Scer\GAL4^elav.PLu

neuroanatomy defective, with Scer\GAL4^GMR.PF

unc-5^UAS.cKa

neuroanatomy defective, with Scer\GAL4^NP6558

unc-5^UAS.cKa

neuroanatomy defective (with Df(2R)XTE-18)

unc-5⁸

neuroanatomy defective (with unc-5²)

unc-5⁸

neuroanatomy defective (with unc-5⁸)

unc-5²

neuroanatomy defective | embryonic stage

unc-5⁸

neuroanatomy defective | embryonic stage, with Scer\GAL4^repo

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

unc-5^GD3510

Phenotype manifest in

Allele

abdominal segmental nerve

abdominal segmental nerve (with Df(2R)XTE-18)

unc-5⁸

abdominal segmental nerve (with unc-5²)

unc-5⁸

abdominal segmental nerve (with unc-5⁸)

unc-5²

anterior commissure, with Scer\GAL4^elav.PLu

cell body & serotonin neuron, with Scer\GAL4^eg-Mz360

unc-5^UAS.cKa

embryonic/larval neuron, with Scer\GAL4^ap-md544

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

embryonic heart cardioblast

unc-5⁸

embryonic peripheral glial cell 5 | embryonic stage

unc-5⁸

embryonic peripheral glial cell 6 | embryonic stage

unc-5⁸

embryonic peripheral glial cell 8 | embryonic stage

unc-5⁸

EW neuron, with Scer\GAL4^eg-Mz360

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

filopodium | embryonic stage

unc-5⁸

heart primordium

unc-5⁸

interface glial cell

unc-5^UAS.cKa

interface glial cell | embryonic stage, with Scer\GAL4^repo

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

intersegmental nerve

intersegmental nerve, with Scer\GAL4^elav.PLu

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

lamellipodium | embryonic stage

unc-5⁸

longitudinal connective, with Scer\GAL4^NP6558

unc-5^UAS.cKa

peripheral glial cell | embryonic stage

unc-5⁸

photoreceptor cell R7, with Scer\GAL4^GMR.PF

unc-5^UAS.cKa

posterior commissure, with Scer\GAL4^elav.PLu

presumptive embryonic salivary gland, with Scer\GAL4^prd.RG1

unc-5^UAS.cKa

serotonin neuron & ventral nerve cord, with Scer\GAL4^eg-Mz360

unc-5^UAS.cKa

ventral nerve cord, with Scer\GAL4^ap-md544

unc-5^{UAS.Tag:HA,Tag:SS(wg)}

ventral nerve cord commissure

unc-5^UAS.cKa

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

15 of 15

Yes

Yes

13 of 15

No

Yes

13 of 15

No

Yes

12 of 15

No

Yes

4 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

13 of 15

No

Yes

13 of 15

No

Yes

12 of 15

No

Yes

4 of 15

No

Yes

Rattus norvegicus (Norway rat) (5)

12 of 13

Yes

Yes

12 of 13

Yes

Yes

11 of 13

No

Yes

9 of 13

No

Yes

3 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (4)

9 of 12

Yes

Yes

8 of 12

No

Yes

8 of 12

No

Yes

2 of 12

No

Yes

Danio rerio (Zebrafish) (6)

10 of 15

Yes

Yes

10 of 15

Yes

Yes

8 of 15

No

Yes

8 of 15

No

Yes

Drer\si:ch73-72b7.1

7 of 15

No

Yes

5 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

13 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901XL )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00005505

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500VC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY010867

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08685

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00UQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012668

Danaus plexippus

Monarch butterfly

Dple\DPOGS211421-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS210087-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005676

Heliconius melpomene

Postman butterfly

Hmel\HMEL011789

Heliconius melpomene

Postman butterfly

Hmel\HMEL012726

Apis florea

Little honeybee

Aflo\4271915716

Apis florea

Little honeybee

Aflo\3926941380

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0024c1

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0024c6

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG020101

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM590880

Rhodnius prolixus

Kissing bug

Rpro\RPRC015335

Rhodnius prolixus

Kissing bug

Rpro\RPRC009007

Cimex lectularius

Bed bug

Clec\CLEC009194

Cimex lectularius

Bed bug

Clec\CLEC009193

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI29381

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04593

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X019B )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR000410

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62729.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62730.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g04680

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g04730

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_46926

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_49901

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01JM )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100176438

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UNC5C; unc-5 netrin receptor C

15 of 15

UNC5A; unc-5 netrin receptor A

13 of 15

UNC5B; unc-5 netrin receptor B

13 of 15

UNC5D; unc-5 netrin receptor D

12 of 15

UNC5CL; unc-5 family C-terminal like

4 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

enzyme linked immunosorbent assay, surface plasmon resonance

(Ozkan et al., 2013, Ozkan et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

Nrg|beat-Ia|unc-5

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

enhanceable

Fas2|Nrg|beat-Ia

(Zarin et al., 2014)

suppressible

(Keleman and Dickson, 2001)

suppressible

(Brankatschk and Dickson, 2006, Labrador et al., 2005)

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

suppressible

(Raza and Jacobs, 2016)

enhanceable

(Raza and Jacobs, 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

suppressible

(Zarin et al., 2014, Labrador et al., 2005)

enhanceable

(Zarin et al., 2014)

suppressible

(Zarin et al., 2014)

enhanceable

(Zarin et al., 2014)

enhanceable

(O'Donnell and Bashaw, 2013)

suppressible

(Zarin et al., 2012)

suppressible

(Raza and Jacobs, 2016)

enhanceable

(Raza and Jacobs, 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62440

DroID - A comprehensive database of gene and protein interactions.

FBgn0034013

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36703

MIST (genetic) - An integrated Molecular Interaction Database

unc-5

MIST (protein-protein) - An integrated Molecular Interaction Database

unc-5

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-74

Cytogenetic map

Sequence location

2R:15,340,472..15,368,300 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51F7-51F11

Limits computationally determined from genome sequence between P{PZ}scb⁰¹²⁸⁸ and P{lacW}dup^k03308

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51F-51F

(Grossberger, 2000.3.23)

Experimentally Determined Recombination Data

Location

2-74

(FlyBase, 2016)

2-78.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

37426

y¹ w^*; Mi{MIC}unc-5^MI04273

42316

y¹ w^*; Mi{MIC}unc-5^MI05371

22946

y¹ w^67c23; Mi{ET1}unc-5^MB00969

60547

y¹ w^67c23; Mi{PT-GFSTF.1}unc-5^{MI05371-GFSTF.1}

33756

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01099}attP2

v110155

P{KK102074}VIE-260B

v8138

w¹¹¹⁸; P{GD3510}v8138

v8137

w¹¹¹⁸; P{GD3510}v8137/TM3

v318261

PBac{fTRG00906.sfGFP-TVPTBF}VK00033

v342254

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01938}attP40/CyO-GFP

Genomic Clones (30)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034013

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034013

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36703

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Albrecht et al., 2011, Keleman and Dickson, 2001)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: unc-5 CG8166 CG8168

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of unc-5 CG8166 CG8168 was sequence comparison ( date:000607 ).

(FlyBase, 1996-)

Other Comments

Overexpression of unc-5 on all CNS axons prevents them from crossing the midline, resulting in a commissureless phenotype. This gain-of-function Unc5d phenotype requires Netrin function. Overexpression of unc-5 in specific interneurons is also sufficient to force them out of the CNS.

(Keleman and Dickson, 2001)

The growth cone response (repulsive) is determined by the cytoplasmic domain of unc-5.

(Keleman and Dickson, 2001)

The unc-5 Netrin receptor mediates long range (with fra) and short range (either acting alone or with a co-receptor other than fra) repulsive responses to Netrin. Midline repulsion mediated by unc-5 operates independently of that mediated by sli and robo.

(Keleman and Dickson, 2001)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36703

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q95TU8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LFH9

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034013

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8166

Flygut - An atlas of the Drosophila adult midgut

FBgn0034013

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36703

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8166

modMine - A data warehouse for the modENCODE project

FBgn0034013

Linkouts

BioGRID - A database of protein and genetic interactions.

62440

DroID - A comprehensive database of gene and protein interactions.

FBgn0034013

DRSC - Results frm RNAi screens

FBgn0034013

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034013

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8166-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034013

FlyMine - An integrated database for Drosophila genomics

FBgn0034013

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36703

MIST (genetic) - An integrated Molecular Interaction Database

unc-5

MIST (protein-protein) - An integrated Molecular Interaction Database

unc-5

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

AAF7419

(Vernooy et al., 2000)

CG8166

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Krejcí et al., 2009, Vogel et al., 2003)

CG8168

CT20824

(Hynes and Zhao, 2000, Hynes and Zhao, 2000, Hynes and Zhao, 2000)

Dunc5

(Grossberger, 2000.3.23)

UNC-5

(Losada-Perez, 2018, Chilton, 2006)

UNC5

(Bravo-Ambrosio and Kaprielian, 2011, Grunwald and Klein, 2002)

UNC5D

(Keleman and Dickson, 2001)

Unc-5

(Kang et al., 2019, Seyres et al., 2016, O'Donnell and Bashaw, 2013, Ozkan et al., 2013, Labrador, 2005, Labrador et al., 2005, Chotard and Salecker, 2004)

Unc5

(Koch et al., 2017, Ocorr et al., 2014, Zarin et al., 2014, Lee et al., 2013, Ozkan et al., 2013, Nose, 2012, Hakeda-Suzuki et al., 2011, Hakeda-Suzuki et al., 2011, Brankatschk and Dickson, 2006, Terman et al., 2002, Keleman and Dickson, 2001, McKinnon et al., 2001)

Unc5d

(Couch et al., 2004, Keleman and Dickson, 2001)

dUnc5

(Gupta et al., 2014)

unc5

(Suzuki et al., 2018, Altenhein et al., 2016, Gupta et al., 2016, Raza and Jacobs, 2016, Pézeron et al., 2014, Macabenta et al., 2013, Zarin et al., 2012, Albrecht et al., 2011, Meyer et al., 2011, von Hilchen et al., 2010, Zlatic et al., 2009, Garbe et al., 2007, Hughes and Thomas, 2007)

Name Synonyms

UNC5 protein

(Banyai and Patthy, 2004)

Unc-5

(Zarin et al., 2014)

Uncoordinated5

(Albrecht et al., 2011)

unc-5

(Lipatov et al., 2005)

unc-5-like

(Hynes and Zhao, 2000, Hynes and Zhao, 2000, Hynes and Zhao, 2000)

uncoordinated 5

(von Hilchen et al., 2010)

Secondary FlyBase IDs

FBgn0034014

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (106)