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Gene Dmel\unc-5

General Information
Symbol	Dmel\unc-5	Species	D. melanogaster
Name	unc-5	Annotation symbol	CG8166
Feature type	protein_coding_gene	FlyBase ID	FBgn0034013
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	UNC5, Unc5d, CT20824
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	51F9-51F11	Sequence location	2R:11,227,977..11,255,805 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene unc-5 is referred to in FlyBase by the symbol Dmel\unc-5 (CG8166, FBgn0034013). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: netrin receptor activity involved in chemorepulsion; netrin receptor activity. There is experimental evidence that it is involved in the biological process: motor neuron axon guidance; salivary gland boundary specification; axon guidance; glial cell migration. 25 alleles are reported. The phenotypes of these alleles are annotated with: presumptive embryonic salivary gland; ventral nerve cord commissure; ventral nerve cord; posterior commissure; intersegmental nerve; interface glial cell; longitudinal connective; abdominal segmental nerve; embryonic/larval neuron; anterior commissure. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Death domain; Death-like domain; Immunoglobulin I-set; Immunoglobulin subtype 2; Immunoglobulin-like domain; Immunoglobulin-like fold; Thrombospondin, type 1 repeat; ZU5. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval trachea. Comments on Affy2 ProbeSet: ProbeSet 1632550_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of unc-5. Gene sequence location is 2R:11227977..11255805.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms intersegmental nerve branch ISNb of A1-7
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 51F9-51F11   Limits computationally determined from genome sequence between P{PZ}scb01288 and P{lacW}dupk03308
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51F-51F     (Grossberger, 2000.3.23)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\unc-5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) unc-5-RA FBtr0087382  NM_137189   5033   1072 unc-5-RB FBtr0339998     5235   1072
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein unc-5-PA   FBpp0086514   116.4   1072   6.09     NP_611033   AAF58143 unc-5-PB   FBpp0309017   116.4   1072   6.09
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Death domain (IPR000488) Thrombospondin, type 1 repeat (IPR000884) ZU5 (IPR000906) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin-like domain (IPR007110) Death-like domain (IPR011029) Immunoglobulin-like fold (IPR013783) Immunoglobulin I-set (IPR013098)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540060   AE013599 AAF58143   AF247762 AAF74193   AI134401   AI532579   AW942530   AY058501 AAL13730   AY061584   BF496289   BG639668   BG640540   BI358610   BI361302   BI638528   CO154616   CO155622   CO155885   CO156049   CO189261   CO189265   CO196503   CO272107   CO291497   CO300805   CO304945   CO305373   CO305496   CO312372   CO324524   CO328157   CO337388   CO337855   CZ490439   EC080309   EC088658   EC091121   EC212154   EC214221   EC222897   EC229322   EC235409   EC237876   EC240795   EC248131   EC249258   EC252887
UniProtKB/Swiss-Prot	Q95TU8
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   mesoderm anlage   (Keleman and Dickson, 2001) embryonic stage 6 -- 14   mesoderm   (Keleman and Dickson, 2001) embryonic stage 13 -- 14   dorsal vessel primordium   (Keleman and Dickson, 2001) embryonic stage 13 -- 17   presumptive embryonic/larval central nervous system | restricted   (Keleman and Dickson, 2001) embryonic stage 14 -- 17   exit glial cell   (Keleman and Dickson, 2001) peripheral glial cell   (Keleman and Dickson, 2001)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 17   segmental nerve   (Keleman and Dickson, 2001) embryonic stage 16 -- 17   motor neuron of segmental nerve   • axon (Keleman and Dickson, 2001) segmental nerve branch SNa of A1-7   (Keleman and Dickson, 2001) intersegmental nerve branch ISNb of A1-7   (Keleman and Dickson, 2001) segmental nerve branch SNc of A1-7   (Keleman and Dickson, 2001) exit glial cell of segmental nerve   (Keleman and Dickson, 2001) peripheral glial cell of intersegmental nerve   (Keleman and Dickson, 2001) exit glial cell of intersegmental nerve   (Keleman and Dickson, 2001)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (737.3) Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    737.3 Larval Midgut    9.1 Larval Hindgut    11.3 Larval Malpighian Tubules    5.9 Larval Fat Body    14.2 Larval Salivary Gland    5.2 Larval Trachea    176.8 Larval Carcass    48.15 Adult Head    93.3 Adult Eye    43.875 Adult Brain    320.4 Adult Thoracic-Abdominal Ganglion    244.7 Adult Crop    9.6 Adult Midgut    4.9 Adult Hindgut    12.4 Adult Malpighian Tubules    4.6 Adult Fat Body    27.6 Adult Salivary Gland    10.6 Adult Heart    17.375 Adult VirginFemale Spermatheca    17.4 Adult InseminatedFemale Spermatheca    24 Adult Ovary    8.2 Adult Testis    17.4 Adult Male Accessory Gland    10.2 Adult Carcass    23.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    93.3   NA  Eye    43.875   NA  Brain    320.4   737.3  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    244.7   NA  Crop    9.6   9.1  Midgut    4.9   11.3  Hindgut    12.4   5.9  Malpighian Tubules    4.6   14.2  Fat Body    27.6   5.2  Salivary Gland    no informative data   NA  Heart    17.375   176.8  Trachea    NA   NA  VirginFemale Spermatheca    17.4   NA  InseminatedFemale Spermatheca    24   NA  Ovary    8.2   NA  Testis    17.4   NA  Male Accessory Gland    no informative data   48.15  Carcass    23.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    23 central nervous system, pupae P8    21 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    5 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    9 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr  (42) embryo 06-08hr  (98) embryo 08-10hr  (80) embryo 10-12hr  (48) embryo 12-14hr  (32) embryo 14-16hr  (35) embryo 16-18hr  (29) embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr  (42) embryo 06-08hr  (98) embryo 08-10hr  (80) embryo 10-12hr  (48) embryo 12-14hr  32 embryo 14-16hr  (35) embryo 16-18hr  29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    16 embryo 04-06hr    42 embryo 06-08hr    98 embryo 08-10hr    80 embryo 10-12hr    48 embryo 12-14hr    32 embryo 14-16hr    35 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    9 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    17 pupae 3d postWPP    12 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    7 adult male 30day    8 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167  26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32  (32) haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11  (27) antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167  26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32  32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11  27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    17 embryonic 1182-4H    14 embryonic GM2    0 embryonic Kc167    26 embryonic S1    9 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    22 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    1 wing disc ML-DmD32    32 haltere disc ML-DmD17-c3    11 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034013 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8166-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4scrt-11-6 unc-5GS15081 viable, with Scer\GAL4pnr-MD237 unc-5GD3510 Other Phenotypes Allele locomotor rhythm defective, with Scer\GAL45-40 unc-5Scer\UAS.cKa neuroanatomy defective unc-51 unc-52 unc-56 unc-58 unc-5Scer\UAS.cKa unc-5unspecified neuroanatomy defective, with Scer\GAL45-40 unc-5Scer\UAS.cKa neuroanatomy defective, with Scer\GAL4ap-md544 unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg neuroanatomy defective, with Scer\GAL4ato-NP6558 unc-5Scer\UAS.cKa neuroanatomy defective, with Scer\GAL4elav.PLu unc-5Scer\UAS.cKa unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg neuroanatomy defective (with Df(2R)XTE-18) unc-58 neuroanatomy defective (with unc-52) unc-58 neuroanatomy defective (with unc-58) unc-52 neuroanatomy defective | embryonic stage unc-58 neuroanatomy defective | embryonic stage, with Scer\GAL4repo unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg Phenotype manifest in Allele abdominal segmental nerve unc-5unspecified unc-58 unc-51 unc-52 unc-56 abdominal segmental nerve (with Df(2R)XTE-18) unc-58 abdominal segmental nerve (with unc-52) unc-58 abdominal segmental nerve (with unc-58) unc-52 anterior commissure, with Scer\GAL4elav.PLu unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg unc-5Scer\UAS.cKa cell body & serotonin neuron, with Scer\GAL4eg-Mz360 unc-5Scer\UAS.cKa embryonic/larval neuron, with Scer\GAL4ap-md544 unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg embryonic peripheral glial cell 5 | embryonic stage unc-58 embryonic peripheral glial cell 6 | embryonic stage unc-58 embryonic peripheral glial cell 8 | embryonic stage unc-58 interface glial cell unc-5Scer\UAS.cKa interface glial cell | embryonic stage, with Scer\GAL4repo unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg intersegmental nerve unc-5unspecified intersegmental nerve, with Scer\GAL4elav.PLu unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg longitudinal connective, with Scer\GAL4ato-NP6558 unc-5Scer\UAS.cKa peripheral glial cell | embryonic stage unc-58 posterior commissure, with Scer\GAL4elav.PLu unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg unc-5Scer\UAS.cKa presumptive embryonic salivary gland, with Scer\GAL4prd.RG1 unc-5Scer\UAS.cKa serotonin neuron & ventral nerve cord, with Scer\GAL4eg-Mz360 unc-5Scer\UAS.cKa ventral nerve cord, with Scer\GAL4ap-md544 unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg ventral nerve cord commissure unc-5Scer\UAS.cKa
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of unc-5 Class Mutagen Stocks Known lesion unc-5f07706 piggyBac transposase 1 -- unc-5MB00969 1 -- unc-5MI04273 1 -- unc-5MI05371 1 -- unc-51 loss of function allele ethyl methanesulfonate 0 Yes unc-52 loss of function allele ethyl methanesulfonate 0 Yes unc-53 ethyl methanesulfonate 0 Yes unc-56 loss of function allele ethyl methanesulfonate 0 -- unc-58 loss of function allele ethyl methanesulfonate 0 Yes unc-5GS15081 P-element activity 0 Yes unc-5unspecified 0 --
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of unc-5 Class Mutagen Stocks Known lesion unc-5GD3510 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes unc-5HMS01099 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes unc-5dsRNA.cKa in vitro construct - RNAi 0 Yes unc-5KK102074 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes unc-5NIG.8166R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes unc-5Scer\UAS.cKa in vitro construct - regulatory fusion 0 Yes unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes unc-5ΔDB.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔDD.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔIg1.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔIg2.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔTSP1.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔTSP2.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes unc-5ΔZU5.Scer\UAS.T:Ivir\HA1,T:SS-wg in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3510} NA P{KK102074} NA P{NIG.8166R} NA P{TRiP.HMS01099} NA P{UAS-unc-5::wg.HA1} NA P{UAS-unc-5.K} NA P{UAS-unc-5ΔDB::wg.HA1} NA P{UAS-unc-5ΔDD::wg.HA1} NA P{UAS-unc-5ΔIg1::wg.HA1} NA P{UAS-unc-5ΔIg2::wg.HA1} NA P{UAS-unc-5ΔTSP1::wg.HA1} NA P{UAS-unc-5ΔTSP2::wg.HA1} NA P{UAS-unc-5ΔZU5::wg.HA1} NA characterization construct P{UAS-fra-unc-5.K} NA P{UAS-unc-5.Myr} NA P{UAS-unc-5-fra.K} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH1635SH1635 No miscellaneous insertions Mi{MIC}unc-5MI04273 NA Mi{MIC}unc-5MI05371 NA PBac{WH}unc-5f07706 NA insertion of enhancer trap binary system Mi{ET1}unc-5MB00969 No insertion of mobile activating element P{GSV6}unc-5GS15081 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon guidance inferred from genetic interaction with NetA (Brankatschk and Dickson, 2006) inferred from genetic interaction with NetB (Brankatschk and Dickson, 2006) inferred from genetic interaction with NetB AND inferred from mutant phenotype (Keleman and Dickson, 2001) inferred from mutant phenotype (Brankatschk and Dickson, 2006) glial cell migration inferred from mutant phenotype (Freeman et al., 2003, von Hilchen et al., 2010) motor neuron axon guidance inferred from mutant phenotype (Labrador et al., 2005, Labrador, 2005) salivary gland boundary specification inferred from mutant phenotype (Kolesnikov and Beckendorf, 2005)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
netrin receptor activity inferred from sequence or structural similarity (Grossberger, 2000.3.23) netrin receptor activity involved in chemorepulsion inferred from sequence or structural similarity (Keleman and Dickson, 2001)
Biological Process
CV term Evidence References
signal transduction inferred from electronic annotation with InterPro:IPR000488 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions unc-5 allele Gene References unc-5dsRNA.cKa NetB (Keleman and Dickson, 2001) unspecified fra (Keleman and Dickson, 2001) NetA (Keleman and Dickson, 2001, Labrador et al., 2005) NetB (Labrador et al., 2005) sli (Keleman and Dickson, 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62440 DroID - A comprehensive database of gene and protein interactions. • FBgn0034013 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36703
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6TQMVB Dipteran inclusive ortholog search EOG6V43GP Insect inclusive ortholog search EOG6ZW47K Arthropod inclusive ortholog search EOG6SN03K Metazoa inclusive ortholog search EOG6R873H
Orthologs in Drosophila Species (EOG6TQMVB)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\unc-5     Drosophila simulans   Dsim\GD11111 Y   Drosophila sechellia   Dsec\GM21606 Y   Drosophila erecta   Dere\GG20514 Y   Drosophila yakuba   Dyak\GE11698 Y   Drosophila ananassae   Dana\GF13032 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20860 Y   Drosophila persimilis   Dper\GL16941 Y   Drosophila willistoni   Dwil\GK10614 Y   Drosophila virilis   Dvir\unc-5 Y   Drosophila mojavensis   Dmoj\GI19611 Y   Drosophila grimshawi   Dgri\GH20429 Y
Orthologs in non-Drosophila Dipterans (EOG6V43GP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008695   Anopheles gambiae Malaria mosquito  Agam\AGAP009061   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010093
Orthologs in non-Dipteran Insects (EOG6ZW47K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB56032   Apis mellifera Western honey bee  Amel\GB47918   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG020101   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13965   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13972   Atta cephalotes Leafcutter ant  Acep\ACEP12815   Atta cephalotes Leafcutter ant  Acep\ACEP16279   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17731   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17750   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12492   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12525   Linepithema humile Argentine ant  Lhum\LH22319   Linepithema humile Argentine ant  Lhum\LH22317   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19610   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19615   Solenopsis invicta Red fire ant  Sinv\SINV19513   Solenopsis invicta Red fire ant  Sinv\SINV19457   Acyrthosiphon pisum Pea aphid  Apim\ACYPI29381   Bombyx mori Silkmoth  Bmor\BGIBMGA012668   Bombyx mori Silkmoth  Bmor\BGIBMGA012735   Bombyx mori Silkmoth  Bmor\BGIBMGA012667   Bombyx mori Silkmoth  Bmor\BGIBMGA010901   Pediculus humanus Human body louse  Phum\PHUM590880   Pediculus humanus Human body louse  Phum\PHUM272880   Pediculus humanus Human body louse  Phum\PHUM273190   Tribolium castaneum Red flour beetle  Tcas\unc-5
Orthologs in non-Insect Arthropods (EOG6SN03K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_46926
Orthologs in non-Arthropod Metazoa (EOG6R873H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\unc-5   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010776gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11111 Drosophila sechellia Dsec\GM21606 Drosophila erecta Dere\GG20514 Drosophila yakuba Dyak\GE11698 Drosophila ananassae Dana\GF13032 Drosophila pseudoobscura pseudoobscura Dpse\GA20860 Drosophila persimilis Dper\GL16941 Drosophila willistoni Dwil\GK10614 Drosophila virilis Dvir\unc-5 Drosophila mojavensis Dmoj\GI19611 Drosophila grimshawi Dgri\GH20429
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	37426 y1 w*; Mi{MIC}unc-5MI04273 42316 y1 w*; Mi{MIC}unc-5MI05371 22946 y1 w67c23; Mi{ET1}unc-5MB00969 33756 y1 sc* v1; P{TRiP.HMS01099}attP2
Harvard	- PBac{WH}unc-5f07706
VDRC	v8138 w1118; P{GD3510}v8138 v8137 w1118; P{GD3510}v8137/TM3
Genomic Clones ( 2 )
	BACR03D24 BACR33K06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD19406 SD03311
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT10053 GH11860 RE45439 RE45463 SD11580 SD20833
Other clones	54343 66195 69891 101632 117665 123960 145593 146005 214477.66 CK01.26A.C1 CK01.26A.E1 CK01.26B.E1 CK01.116B.E11 EK005015 EK043711 EK043719 EK105909 EK179435 EK194030 EK197517 EK228561 EK246761 EK248403 EK248817 EN04456 EN06436 EN06923 EN07246 EN14431 EN15434 EP08653 SD04136
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034013 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36703
Antibody Information
	polyclonal (Albrecht et al., 2011, Keleman and Dickson, 2001)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: unc-5 CG8166 CG8168 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of unc-5 CG8166 CG8168 was sequence comparison (date:000607). (FlyBase, 1996-)
Other Comments
	Overexpression of unc-5 on all CNS axons prevents them from crossing the midline, resulting in a commissureless phenotype. This gain-of-function Unc5d phenotype requires Netrin function. Overexpression of unc-5 in specific interneurons is also sufficient to force them out of the CNS. (Keleman and Dickson, 2001) The growth cone response (repulsive) is determined by the cytoplasmic domain of unc-5. (Keleman and Dickson, 2001) The unc-5 Netrin receptor mediates long range (with fra) and short range (either acting alone or with a co-receptor other than fra) repulsive responses to Netrin. Midline repulsion mediated by unc-5 operates independently of that mediated by sli and robo. (Keleman and Dickson, 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137189 RefSeq (Proteins) • NP_611033 DDBJ / EMBL / GenBank • AA540060 AAF58143 AAF74193 AAL13730 AF247762 AI532579 AW942530 AY058501 BG639668 CZ490439 Entrez Gene - A searchable database of RefSeq genes. • 36703 UniProtKB/Swiss-Prot • Q95TU8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Death domain (IPR000488) Thrombospondin, type 1 repeat (IPR000884) ZU5 (IPR000906) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin-like domain (IPR007110) Death-like domain (IPR011029) Immunoglobulin-like fold (IPR013783) Immunoglobulin I-set (IPR013098)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62440 DroID - A comprehensive database of gene and protein interactions. • FBgn0034013 DRSC - Results from RNAi screens. • FBgn0034013 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034013 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8166-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034013 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36703 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/unc5-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36703 modMine - Data generated by the modENCODE project. • FBgn0034013
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	AAF7419 (Vernooy et al., 2000) CG8166 (Vogel et al., 2003, Krejcí et al., 2009, Ni et al., 2010.12.1, Keleman et al., 2009.8.5) CG8168   CT20824 (Hynes and Zhao, 2000, Hynes and Zhao, 2000, Hynes and Zhao, 2000) Dunc5 (Grossberger, 2000.3.23) Unc-5 (Labrador et al., 2005, Chotard and Salecker, 2004, Labrador, 2005) UNC5 (Grunwald and Klein, 2002) Unc5 (Terman et al., 2002, McKinnon et al., 2001, Keleman and Dickson, 2001, Hakeda-Suzuki et al., 2011, Hakeda-Suzuki et al., 2011, Brankatschk and Dickson, 2006) unc-5 (Akdemir et al., 2007, Southall and Brand, 2009, Garbe et al., 2007, Ayroles et al., 2009, Lipatov et al., 2005, Krejcí et al., 2009, von Hilchen et al., 2010, Hazelett et al., 2009, Japanese National Institute of Genetics, 2012.5.21) UNC-5 (Chilton, 2006) unc5 (Hughes and Thomas, 2007, Albrecht et al., 2011, Zlatic et al., 2009, Garbe et al., 2007, von Hilchen et al., 2010, Zarin et al., 2012, Meyer et al., 2011) Unc5d (Couch et al., 2004, Keleman and Dickson, 2001) UNC5D (Keleman and Dickson, 2001)
Name Synonym	unc-5 (Lipatov et al., 2005) unc-5-like (Hynes and Zhao, 2000, Hynes and Zhao, 2000, Hynes and Zhao, 2000) UNC5 protein (Banyai and Patthy, 2004) Uncoordinated5 (Albrecht et al., 2011) uncoordinated 5 (von Hilchen et al., 2010)
Secondary FlyBase IDs
	FBgn0034014
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 26 ) Supplementary material  ( 9 ) Review  ( 5 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Housden et al., 2013, PLoS Genet. 9(1): e1003162 Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. [FBrf0220474] Zarin et al., 2012, Development 139(10): 1798--1805 A GATA/homeodomain transcriptional code regulates axon guidance through the Unc-5 receptor. [FBrf0218039] Albrecht et al., 2011, Dev. Biol. 350(1): 89--100 The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster. [FBrf0212758] Hakeda-Suzuki et al., 2011, Nat. Neurosci. 14(3): 314--323 Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system. [FBrf0213128] Show all research papers ...
Recent reviews ( 1 )
	Meyer et al., 2011, Europ. J. Cell Biol. 90(9): 770--778 Drosophila metalloproteases in development and differentiation: The role of ADAM proteins and their relatives. [FBrf0214506] Show all reviews ...