FlyBase Gene Report: Dmel\Tmem131

General Information

Symbol

Dmel\Tmem131

Species

D. melanogaster

Name

Transmembrane protein 131

Annotation Symbol

CG8370

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034060

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Collagen binding transmembrane protein involved in collagen secretion, probably by recruiting the ER-to-Golgi transport complex TRAPPIII. (UniProt, Q9V7H4)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

52D11-52D12

Sequence location

2R:16,001,181..16,006,901 [+]

Recombination map

2-76

RefSeq locus

NT_033778 REGION:16001181..16006901

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in collagen biosynthetic process

inferred from mutant phenotype

(Zhang et al., 2020)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000498719

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the TMEM131 family. (Q9V7H4)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Collagen binding transmembrane protein involved in collagen secretion, probably by recruiting the ER-to-Golgi transport complex TRAPPIII.

(UniProt, Q9V7H4)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tmem131 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V7H4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tmem131-RA

FBtr0087203

NM_137229

5610

1567

Tmem131-RB

FBtr0340015

NM_001299542

5030

1567

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tmem131-PA

FBpp0086345

173.3

1567

8.69

FBpp0309029

173.3

1567

8.69

Q9V7H4

NP_001286471

AHN56268

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1567 aa isoforms: Tmem131-PA, Tmem131-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

May interact (via PapD-L domain) with collagen proteins (via C-terminus); the interaction is direct and is involved in assembly and TRAPPIII ER-to-Golgi transport complex-dependent secretion of collagen.

(UniProt, Q9V7H4)

Domain

Possesses a PapD-like (PapD-L) domain similar to PapD domains involved in assembly and secretion of bacterial pilus components (PubMed:32095531). The PapD-L domain can bind collagens and is probably involved in collagen assembly and secretion (PubMed:32095531).

(UniProt, Q9V7H4)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V7H4

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tmem131 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8370_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.19

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Arbeitman et al., 2004)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

germline cell

(Arbeitman et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tmem131 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tmem131

View exonic expression by tissue for Dmel\Tmem131

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034060

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36761/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034060

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034060

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034060

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8370

Flygut - An atlas of the Drosophila adult midgut

FBgn0034060

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tmem131

Transgenic constructs containing regulatory region of Tmem131

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel7138

(Ryder, 2004.4.26)

Df(2R)Exel7139

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Tub.PU

Tmem131^GD527

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Tmem131^GD527

viable

Tmem131^EY09141

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tmem131^GD527

viable, with Scer\GAL4^tin.CΔ4

Tmem131^GD527

Sterility

Allele

fertile

Tmem131^EY09141

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

Tmem131^GD527

visible, with Scer\GAL4^pnr-MD237

Tmem131^GD527

Phenotype manifest in

Allele

fat cell | third instar larval stage, with Scer\GAL4^Lsp2.PU

Tmem131^GD527

mesonotum, with Scer\GAL4^pnr-MD237

Tmem131^GD527

trichogen cell, with Scer\GAL4^pnr-MD237

Tmem131^GD527

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\TMEM131

12 of 14

Yes

Hsap\TMEM131L

3 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Tmem131

12 of 14

Yes

Rnor\Tmem131l

3 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Tmem131

12 of 14

Yes

Mmus\Tmem131l

3 of 14

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\tmem131

9 of 13

Yes

Xtro\rps19bp1

2 of 13

Yes

Xtro\tmem131l

1 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\tmem131

12 of 14

Yes

Drer\tmem131l

4 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\tmem-131

11 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP008098

10 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT5G66820

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tmem131. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

dpy

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TMEM131; transmembrane protein 131

12 of 14

615659

TMEM131L; transmembrane 131 like

3 of 14

616243

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9V7H4 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0034060

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-76

Cytogenetic map

52D11-52D12

Sequence location

2R:16,001,181..16,006,901 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52D11-52D12

Limits computationally determined from genome sequence between P{lacW}l(2)k02205^k02205&P{PZ}sli⁰⁵²⁴⁸ and P{lacW}ATPCL^k09217

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-76

(FlyBase, 2016)

2-80.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19884

y¹ w^67c23; P{EPgy2}Tmem131^EY09141

14553

y¹ w^67c23; P{SUPor-P}Tmem131^KG07726

Kyoto

103833

w^*; P{GawB}Tmem131^NP0957 / CyO

NIG-Fly

8370R-3

P{NIG.8370R}3

VDRC

v42509

w¹¹¹⁸; P{GD527}v42509

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (23)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034060

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034060

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Tmem131 CG8370

(Zhang et al., 2020)

Nomenclature comments

Etymology

Named 'Tmem131' after the human ortholog.

(Zhang et al., 2020)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG8370

(Dutta et al., 2024, Zhang et al., 2020, Hosono et al., 2015, Cui et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Tan et al., 2009, Buszczak et al., 2007, Minidorff et al., 2007, Robida et al., 2007, Molnar et al., 2006)

Tmem131

tmem131

(Zhang et al., 2020)

Name Synonyms

Transmembrane protein 131

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36761

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V7H4

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V7H4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V7H4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034060

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36761/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034060

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034060

FlyMine - An integrated database for Drosophila genomics

FBgn0034060

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8370

MARRVEL_MODEL - MARRVEL (model organism gene)

36761

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034060

DroID - A comprehensive database of gene and protein interactions.

FBgn0034060

DRSC - Results frm RNAi screens

FBgn0034060

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8370_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034060

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034060

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8370

Flygut - An atlas of the Drosophila adult midgut

FBgn0034060

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008022

References (31)

The following external sites may use different assemblies or annotations than FlyBase.

-0.19

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