Gene Dmel\CG8399

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General Information

Symbol

Dmel\CG8399

Species

D. melanogaster

Name

Annotation symbol

CG8399

Feature type

protein_coding_gene

FlyBase ID

FBgn0034067

Gene Model Status

Current

Stock availability

6 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

52D14-52D14

Sequence location

2R:11,905,242..11,912,132 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG8399 (FBgn0034067). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 5 alleles are reported. The phenotype of these alleles is annotated with: trichogen cell. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Cytochrome b561/ferric reductase transmembrane; DOMON domain; Reeler domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, adult midgut, larval/adult hindgut, larval trachea, adult ovary, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1632377_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8399. Gene sequence location is 2R:11905242..11912132.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52D14-52D14

Limits computationally determined from genome sequence between P{PZ}l(2)05070⁰⁵⁰⁷⁰&P{EP}CG8414^EP525 and P{EP}spin^EP645&P{lacW}spin^k09905

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG8399 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG8399-RA

FBtr0087207

NM_137235

2315

647

CG8399-RB

FBtr0340018

2342

656

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG8399-PA

FBpp0086349

70.3

647

8.12

NP_611079

AAF58074

CG8399-PB

FBpp0309032

71.3

656

8.08

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Reeler domain (IPR002861)

DOMON domain (IPR005018)

Cytochrome b561/ferric reductase transmembrane (IPR006593)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8MSU3

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, adult midgut, larval/adult hindgut, larval trachea, adult ovary, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		40.15
Larval Midgut		72.7
Larval Hindgut		478.6
Larval Malpighian Tubules		4.1
Larval Fat Body		8
Larval Salivary Gland		37
Larval Trachea		434.8
Larval Carcass		478.1
Adult Head		117.4
Adult Eye		60.575
Adult Brain		18.6
Adult Thoracic-Abdominal Ganglion		22.9
Adult Crop		456.5
Adult Midgut		119.3
Adult Hindgut		242.9
Adult Malpighian Tubules		14.4
Adult Fat Body		21.4
Adult Salivary Gland		57.5
Adult Heart		26.775
Adult VirginFemale Spermatheca		24.3
Adult InseminatedFemale Spermatheca		28.2
Adult Ovary		111.1
Adult Testis		9.7
Adult Male Accessory Gland		50.4
Adult Carcass		173.7
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	117.4
NA	Eye	60.575
NA	Brain	no informative data
40.15	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	no informative data
NA	Crop	456.5
72.7	Midgut	119.3
478.6	Hindgut	242.9
4.1	Malpighian Tubules	no informative data
8	Fat Body	no informative data
37	Salivary Gland	no informative data
NA	Heart	26.775
434.8	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	111.1
NA	Testis	9.7
NA	Male Accessory Gland	no informative data
478.1	Carcass	173.7

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		(32)
head, mated 4-day male		(32)
head, mated 20-day male		(44)
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		(77)
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		(37)
ovary, mated 4-day female		(50)
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		(44)
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		(77)
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		(37)
ovary, mated 4-day female		(50)
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		19
central nervous system, larvae L3		7
central nervous system, pupae P8		3
head, virgin 1-day female		22
head, virgin 4-day female		20
head, virgin 20-day female		23
head, mated 1-day female		19
head, mated 4-day female		18
head, mated 20-day female		17
head, mated 1-day male		32
head, mated 4-day male		32
head, mated 20-day male		44
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		11
digestive system, 1-day adult		22
digestive system, 4-day adult		21
digestive system, 20-day adult		19
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		2
carcass, larvae L3 wandering		77
carcass, 1-day adult		22
carcass, 4-day adult		24
carcass, 20-day adult		17
ovary, virgin 4-day female		37
ovary, mated 4-day female		50
testis, mated 4-day male		2
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		184
embryo 20-22hr		111
embryo 22-24hr		115
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		120
pupae 4d postWPP		120
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(40)
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		(40)
embryo 16-18hr		(89)
embryo 18-20hr		(184)
embryo 20-22hr		(111)
embryo 22-24hr		(115)
larva L1		(57)
larva L2		(36)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		(33)
larva L3 puffstage 3-6		(49)
larva L3 puffstage 7-9		(44)
white prepupae new		22
white prepupae 12hr		(71)
white prepupae 24hr		26
pupae 2d postWPP		(31)
pupae 3d postWPP		(120)
pupae 4d postWPP		(120)
adult male 01day		(27)
adult male 05day		23
adult male 30day		(29)
adult female 01day		(42)
adult female 05day		(41)
adult female 30day		(43)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		(184)
embryo 20-22hr		(111)
embryo 22-24hr		(115)
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		(120)
pupae 4d postWPP		(120)
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		184
embryo 20-22hr		111
embryo 22-24hr		115
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		120
pupae 4d postWPP		120
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		184
embryo 20-22hr		111
embryo 22-24hr		115
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		120
pupae 4d postWPP		120
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(40)
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		(40)
embryo 16-18hr		(89)
embryo 18-20hr		(184)
embryo 20-22hr		(111)
embryo 22-24hr		(115)
larva L1		(57)
larva L2		(36)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		(33)
larva L3 puffstage 3-6		(49)
larva L3 puffstage 7-9		(44)
white prepupae new		22
white prepupae 12hr		(71)
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		(120)
pupae 4d postWPP		(120)
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		(42)
adult female 05day		(41)
adult female 30day		(43)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		(184)
embryo 20-22hr		111
embryo 22-24hr		115
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		120
pupae 4d postWPP		120
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		3
embryo 04-06hr		1
embryo 06-08hr		3
embryo 08-10hr		8
embryo 10-12hr		21
embryo 12-14hr		15
embryo 14-16hr		40
embryo 16-18hr		89
embryo 18-20hr		184
embryo 20-22hr		111
embryo 22-24hr		115
larva L1		57
larva L2		36
larva L3 12hr old		38
larva L3 puffstage 1-2		33
larva L3 puffstage 3-6		49
larva L3 puffstage 7-9		44
white prepupae new		22
white prepupae 12hr		71
white prepupae 24hr		26
pupae 2d postWPP		31
pupae 3d postWPP		120
pupae 4d postWPP		120
adult male 01day		27
adult male 05day		23
adult male 30day		29
adult female 01day		42
adult female 05day		41
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		(30)
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		(34)
wing disc ML-DmD8		8
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(37)
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		(33)
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		(41)
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		(33)
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		(34)
wing disc ML-DmD8		8
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(37)
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		(33)
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		(41)
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		(33)
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		10
Schneider line 2 Sg4		24
embryonic 1182-4H		8
embryonic GM2		4
embryonic Kc167		15
embryonic S1		30
embryonic S3		13
leg disc CME L1		24
wing disc CME-W2		34
wing disc ML-DmD8		8
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		15
wing disc ML-DmD32		14
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		33
antennal disc ML-DmD20-c5		6
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		41
CNS ML-DmBG2-c2		16
tumorous blood cells mbn2		0
ovary fGS/OSS		33
ovary OSC		11
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		(27)
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		(27)
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(32)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(37)
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		(36)
Paraquat 10 mM 48 hrs, 4-day adult		(40)
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(37)
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		(36)
Paraquat 10 mM 48 hrs, 4-day adult		(40)
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		16
cold shock, 4-day adult		11
heat shock, 4-day adult		27
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		18
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		24
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		22
Ethanol 10% 3 hrs, larvae L3		20
Caffeine 1.5 mg/ml 4 hrs, larvae L3		32
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		36
Paraquat 10 mM 48 hrs, 4-day adult		40
Rotenone 2 μg 12 hrs, larvae L3		19
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034067

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8399-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG8399^GD2023

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

CG8399^GD2023

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

CG8399^GD2023

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of CG8399	Class	Mutagen	Stocks	Known lesion
CG8399^5-HA-1540			1	--
CG8399^EY22176		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG8399	Mutagen	Stocks	Known lesion
CG8399^GD2023	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG8399^HMS02603	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG8399^KK107311	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)Exel7139

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2023}

P{KK107311}

P{TRiP.HMS02603}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{RS5}CG8399^5-HA-1540

insertion of mobile activating element

P{EPgy2}CG8399^EY22176

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG8399 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0034067

Orthologs

OrthoDB Orthologs (54) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6DNFQ0

Dipteran inclusive ortholog search

EOG6J6S03

Insect inclusive ortholog search

EOG6S1S3D

Arthropod inclusive ortholog search

EOG6H70SV

Metazoa inclusive ortholog search

EOG6HTNK8

Orthologs in Drosophila Species (EOG6DNFQ0)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21047

Drosophila persimilis

Dper\GL17209

Drosophila willistoni

Dwil\GK12452

Drosophila willistoni

Dwil\GK12451

Drosophila virilis

Dvir\GJ22279

Drosophila mojavensis

Dmoj\GI19146

Drosophila grimshawi

Dgri\GH22061

Orthologs in non-Drosophila Dipterans (EOG6J6S03)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006322

Anopheles gambiae

Malaria mosquito

Agam\AGAP005170

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005530

Orthologs in non-Dipteran Insects (EOG6S1S3D)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB40907

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013279

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH19533

Atta cephalotes

Leafcutter ant

Acep\ACEP26878

Atta cephalotes

Leafcutter ant

Acep\ACEP26880

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20533

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL20690

Linepithema humile

Argentine ant

Lhum\LH24883

Linepithema humile

Argentine ant

Lhum\LH24880

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB19143

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB19142

Solenopsis invicta

Red fire ant

Sinv\SINV21976

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009302

Bombyx mori

Silkmoth

Bmor\BGIBMGA013908

Pediculus humanus

Human body louse

Phum\PHUM038630

Tribolium castaneum

Red flour beetle

Tcas\TC009118

Orthologs in non-Insect Arthropods (EOG6H70SV)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Dtymk

Daphnia pulex

Water flea

Dpux\JGI_V11_318464

Daphnia pulex

Water flea

Dpux\JGI_V11_224638

Daphnia pulex

Water flea

Dpux\FLNA

Ixodes scapularis

Deer tick

Isca\ISCW010386

Ixodes scapularis

Deer tick

Isca\ISCW004546

Orthologs in non-Arthropod Metazoa (EOG6HTNK8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\M03A1.8

Caenorhabditis elegans

Nematode

Cele\C13B4.1

Caenorhabditis elegans

Nematode

Cele\F23B12.4

Caenorhabditis elegans

Nematode

Cele\Y57G11A.4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017103gn

Danio rerio

Zebrafish

Drer\ENSDARG00000061481

Danio rerio

Zebrafish

Drer\si:ch211-153l6.2

Danio rerio

Zebrafish

Drer\si:ch211-153l6.2

Danio rerio

Zebrafish

Drer\ENSDARG00000077605

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000013197

Xenopus tropicalis

Western clawed frog

Xtro\frrs1

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000008225

Gallus gallus

Domestic chicken

Ggal\FRRS1

Mus musculus

House mouse

Mmus\ENSMUSG00000033386

Rattus norvegicus

Norway rat

Rnor\Frrs1

Homo sapiens

Human

Hsap\ENSG00000156869

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ENSG00000156869

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21047

Drosophila persimilis

Dper\GL17209

Drosophila willistoni

Dwil\GK12451

Drosophila virilis

Dvir\GJ22279

Drosophila mojavensis

Dmoj\GI19146

Drosophila grimshawi

Dgri\GH22061

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

22542

y¹ w^67c23; P{EPgy2}CG8399^EY22176

42910

y¹ sc^* v¹; P{TRiP.HMS02603}attP40

Kyoto

125248

w¹¹¹⁸; P{RS5}CG8399^5-HA-1540

VDRC

v100680

P{KK107311}VIE-260B

v4898

w¹¹¹⁸; P{GD2023}v4898

v4899

w¹¹¹⁸; P{GD2023}v4899

Genomic Clones ( 1 )

BACR04O19

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 30 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD47639

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0034067

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

36768

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG8399 anon-EST:ParkEST009

(Bayraktaroglu, 2000.11.2)

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_137235

RefSeq (Proteins)

•

NP_611079

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

36768

UniProtKB/Swiss-Prot

•

Q8MSU3

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Reeler domain (IPR002861)

DOMON domain (IPR005018)

Cytochrome b561/ferric reductase transmembrane (IPR006593)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0034067

DRSC - Results from RNAi screens.

•

FBgn0034067

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034067

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8399-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0034067

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

36768

modMine - Data generated by the modENCODE project.

•

FBgn0034067

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

anon-EST:ParkEST009

CG8399

(Kim et al., 2006, Taylor-Harding et al., 2004, Lu et al., 2007, Keleman et al., 2009.8.5, Rehwinkel et al., 2005, Lee et al., 2010, Ni et al., 2010.12.1)

Name Synonym

Secondary FlyBase IDs

FBgn0026300

References ( 20 )

Research paper

Lee et al., 2010, Mol. Cell. Biol. 30(10): 2563--2577: Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. [FBrf0210656]
Lu et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(22): 9381--9386: A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression. [FBrf0201281]
Kim et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(44): 16358--16363: Caspar, a suppressor of antibacterial immunity in Drosophila. [FBrf0194794]
Rehwinkel et al., 2005, RNA 11(10): 1530--1544: Nonsense-mediated mRNA decay factors act in concert to regulate common mRNA targets. [FBrf0190595]
Taylor-Harding et al., 2004, Mol. Cell. Biol. 24(20): 9124--9136: p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes. [FBrf0180538]
Dimova et al., 2003, Genes Dev. 17(18): 2308--2320: Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway. [FBrf0162136]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
Khanna et al., 2010, BMC Genomics 11(1):: Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane. [FBrf0210978]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]

Review

Aravind, 2001, Trends Biochem. Sci. 26(9): 524--526: DOMON: an ancient extracellular domain in dopamine β-monooxygenase and other proteins. [FBrf0182523]

Personal communication to FlyBase

Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila.: A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437]
Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Bellen, 2006.8.3, MBBatch686_2.xml: MBBatch686_2.xml [FBrf0191805]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Bayraktaroglu, 2000.11.2, Current list of possibly CG-able known genes.: Current list of possibly CG-able known genes. [FBrf0131225]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.: Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]