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Gene Dmel\Asph

General Information
Symbol	Dmel\Asph	Species	D. melanogaster
Name	Aspartyl β-hydroxylase	Annotation symbol	CG8421
Feature type	protein_coding_gene	FlyBase ID	FBgn0034075
Gene Model Status	Current	Stock availability	10 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	52E5-52E5	Sequence location	2R:11,997,990..12,004,501 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Aspartyl β-hydroxylase is referred to in FlyBase by the symbol Dmel\Asph (CG8421, FBgn0034075). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: peptide-aspartate beta-dioxygenase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: peptidyl-amino acid modification. 10 alleles are reported. No phenotypic data is available. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Aspartyl/Asparaginyl beta-hydroxylase; Tetratricopeptide repeat-containing; Tetratricopeptide-like helical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval Malpighian tubules, adult heart, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult thoracico-abdominal ganglion, larval/adult hindgut, adult Malpighian tubules, adult male accessory gland. Gene sequence location is 2R:11997990..12004501.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 20 of adulthood day 22 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 23 of adulthood day 35 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood day 34 of adulthood day 44 of adulthood day 14 of adulthood day 46 of adulthood References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Polypeptides Asph-PK Sequence features HFA07171 HFA06783 BKN29349 Transcripts Asph-RK
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 52E5-52E5   Limits computationally determined from genome sequence between P{PZ}l(2)0507005070&P{EP}CG8414EP525 and P{EP}spinEP645&P{lacW}spink09905
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Asph for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Asph-RA FBtr0087220  NM_166140   2746   785 Asph-RB FBtr0087219  NM_079033   3160   382 Asph-RG FBtr0300575  NM_176185   3945   557 Asph-RH FBtr0305090     2800   803 Asph-RI FBtr0305091     4003   575 Asph-RJ FBtr0305092     3214   400 Asph-RK FBtr0310447     3946   556
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Asph-PA   FBpp0086359   89.8   785   4.09     NP_725525   AAM70947 Asph-PB   FBpp0086358   43.3   382   3.62     NP_523757   AAF58063 Asph-PG   FBpp0289802   63.3   557   3.61     NP_788365   AAM70948 Asph-PH   FBpp0293627   91.9   803   4.10       Asph-PI   FBpp0293628   65.3   575   3.63       Asph-PJ   FBpp0293629   45.3   400   3.64       Asph-PK   FBpp0302595   63.1   556   3.61
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Aspartyl/Asparaginyl beta-hydroxylase (IPR007803) Tetratricopeptide-like helical (IPR011990) Tetratricopeptide repeat-containing (IPR013026)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141933   AA141934   AA696179   AE013599 AAF58063   AE013599 AAM70947   AE013599 AAM70948   AC004248   AF289493 AAG40806   AF289494 AAG40807   AW941246   AY060905 AAL28453   BF489148   BF490693   BI352571   BI354709   BI370831   BI375070   BT003633 AAO39637   BT120069   CL528266   CO277514   CO303310   CO323565   CO331602   CO343826   CZ467913   CZ473693   CZ473694   CZ477717   EC067700   EC200677   EC205834   EC220641   EC248931   EC255016
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q86NU6 Q95S93 Q9GQ82 Q7KHE2 A4UZJ1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0034075    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0034075 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0034075 expression level Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new    11149 white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr  (1715) embryo 04-06hr  (3217) embryo 06-08hr  (2070) embryo 08-10hr  (1612) embryo 10-12hr  (1862) embryo 12-14hr  (2789) embryo 14-16hr  (3121) embryo 16-18hr  (4174) embryo 18-20hr  (3434) embryo 20-22hr  (2412) embryo 22-24hr  (2785) larva L1  (2829) larva L2  (3480) larva L3 12hr old  (3433) larva L3 puffstage 1-2  (1512) larva L3 puffstage 3-6  (2019) larva L3 puffstage 7-9  (6206) white prepupae new  (11149) white prepupae 12hr  (5197) white prepupae 24hr  (2577) pupae 2d postWPP  (5695) pupae 3d postWPP  (6675) pupae 4d postWPP  (6255) adult male 01day  (4301) adult male 05day  (2101) adult male 30day  (1784) adult female 01day  (2314) adult female 05day  (453) adult female 30day  (385) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr  (1715) embryo 04-06hr  (3217) embryo 06-08hr  (2070) embryo 08-10hr  (1612) embryo 10-12hr  (1862) embryo 12-14hr  (2789) embryo 14-16hr  (3121) embryo 16-18hr  (4174) embryo 18-20hr  (3434) embryo 20-22hr  (2412) embryo 22-24hr  (2785) larva L1  (2829) larva L2  (3480) larva L3 12hr old  (3433) larva L3 puffstage 1-2  (1512) larva L3 puffstage 3-6  (2019) larva L3 puffstage 7-9  (6206) white prepupae new  (11149) white prepupae 12hr  (5197) white prepupae 24hr  (2577) pupae 2d postWPP  (5695) pupae 3d postWPP  (6675) pupae 4d postWPP  (6255) adult male 01day  (4301) adult male 05day  (2101) adult male 30day  (1784) adult female 01day  (2314) adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new  (11149) white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new    11149 white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0034075 expression level Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new    11149 white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr  (1715) embryo 04-06hr  (3217) embryo 06-08hr  (2070) embryo 08-10hr  (1612) embryo 10-12hr  (1862) embryo 12-14hr  (2789) embryo 14-16hr  (3121) embryo 16-18hr  (4174) embryo 18-20hr  (3434) embryo 20-22hr  (2412) embryo 22-24hr  (2785) larva L1  (2829) larva L2  (3480) larva L3 12hr old  (3433) larva L3 puffstage 1-2  (1512) larva L3 puffstage 3-6  (2019) larva L3 puffstage 7-9  (6206) white prepupae new  (11149) white prepupae 12hr  (5197) white prepupae 24hr  (2577) pupae 2d postWPP  (5695) pupae 3d postWPP  (6675) pupae 4d postWPP  (6255) adult male 01day  (4301) adult male 05day  (2101) adult male 30day  (1784) adult female 01day  (2314) adult female 05day  (453) adult female 30day  (385) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr  1715 embryo 04-06hr  (3217) embryo 06-08hr  2070 embryo 08-10hr  1612 embryo 10-12hr  1862 embryo 12-14hr  (2789) embryo 14-16hr  (3121) embryo 16-18hr  (4174) embryo 18-20hr  (3434) embryo 20-22hr  2412 embryo 22-24hr  (2785) larva L1  (2829) larva L2  (3480) larva L3 12hr old  (3433) larva L3 puffstage 1-2  1512 larva L3 puffstage 3-6  2019 larva L3 puffstage 7-9  (6206) white prepupae new  (11149) white prepupae 12hr  (5197) white prepupae 24hr  (2577) pupae 2d postWPP  (5695) pupae 3d postWPP  (6675) pupae 4d postWPP  (6255) adult male 01day  (4301) adult male 05day  2101 adult male 30day  1784 adult female 01day  2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new  11149 white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    1715 embryo 04-06hr    3217 embryo 06-08hr    2070 embryo 08-10hr    1612 embryo 10-12hr    1862 embryo 12-14hr    2789 embryo 14-16hr    3121 embryo 16-18hr    4174 embryo 18-20hr    3434 embryo 20-22hr    2412 embryo 22-24hr    2785 larva L1    2829 larva L2    3480 larva L3 12hr old    3433 larva L3 puffstage 1-2    1512 larva L3 puffstage 3-6    2019 larva L3 puffstage 7-9    6206 white prepupae new    11149 white prepupae 12hr    5197 white prepupae 24hr    2577 pupae 2d postWPP    5695 pupae 3d postWPP    6675 pupae 4d postWPP    6255 adult male 01day    4301 adult male 05day    2101 adult male 30day    1784 adult female 01day    2314 adult female 05day    453 adult female 30day    385 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0034075    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0034075 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval Malpighian tubules, adult heart, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult thoracico-abdominal ganglion, larval/adult hindgut, adult Malpighian tubules, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034075 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8421-RE;CG8421-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable AsphKG09881 AsphZCL1605 Sterility Allele fertile AsphKG09881 AsphZCL1605 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Asph Class Mutagen Stocks Known lesion AsphZCL1605 P-element activity 2 -- Asphc02390 piggyBac transposase 1 -- AsphCB-6455-3 1 -- Asphd04025 P-element activity 1 -- Asphd11430 P-element activity 1 -- AsphKG09881 P-element activity 1 -- AsphMB12115 1 -- AsphCPTI001828 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Asph Class Mutagen Stocks Known lesion AsphGD4542 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AsphKK109349 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4542} NA P{KK109349} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{PTT-GA}AsphZCL1605 NA P{RS3}AsphCB-6455-3 NA P{SUPor-P}AsphKG09881 NA PBac{681.P.FSVS-1}AsphCPTI001828 NA PBac{PB}Asphc02390 NA insertion of enhancer trap P{lacW}l(2)SH0285SH0285 No insertion of mobile activating element P{XP}Asphd04025 NA P{XP}Asphd11430 NA insertion of enhancer trap binary system Mi{ET1}AsphMB12115 No
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
binding inferred from electronic annotation with InterPro:IPR011990 (FlyBase Curators et al., 2004-) peptide-aspartate beta-dioxygenase activity non-traceable author statement (Dinchuk, 2000.7.25)
Biological Process
CV term Evidence References
peptidyl-amino acid modification inferred from electronic annotation with InterPro:IPR007803 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to endoplasmic reticulum membrane inferred from electronic annotation with InterPro:IPR007803 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Asph allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62502 DroID - A comprehensive database of gene and protein interactions. • FBgn0034075 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36778
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11291 Dere\GG20567 Dgri\GH22068 Dmoj\GI19152 Dper\GL20566 Dpse\GA21066 Dsec\GM21659 Dsim\GD11159 Dvir\GJ22287 Dwil\GK21857 Dyak\GE11752
Curated drosophilid orthologs	Dpse\GA21066
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005181-PA Aedes aegypti (mosquito) AGAP005832-PB Anopheles gambiae (mosquito) BGIBMGA002639-PA Bombyx mori (silk worm) ENSBTAP00000021267 Bos taurus (bovine) CN16304 Caenorhabditis brenneri (small nematode) CE41680 Caenorhabditis elegans (worm) JA08068 Caenorhabditis japonica (nematode) ENSCPOP00000006623 Cavia porcellus (guinea pig) RP43824 Caenorhabditis remanei (nematode) ENSCSAVP00000014978 Ciona savignyi (sedentary tunicate) 168479 Capitella spl (polychaete worm) ENSECAP00000018926 Equus caballus (horse) ENSGALP00000024919 Gallus gallus (chicken) ENSP00000368767 Homo sapiens (human) 144765 Lottia gigantea (owl limpet) 24136 Monocosta brevicollis (choanoflagellate) ENSMODP00000010382 Monodelphis domestica (gray opossum) ENSMMUP00000011501 Macaca mulatta (rhesus monkey) ENSMUSP00000077273 Mus musculus (house mouse) 173452 Nematostella vectensis (starlet anemone) ENSOANP00000008009 Ornithorynchus anatinus (platypus) ENSORLP00000004032 Oryzias latipes (japanese killifish) PHUM001218-PA Pediculus humanus (human body louse) ENSPPYP00000020883 Pongo pygmaeus (orangutan) ENSPTRP00000053155 Pan troglodytes (chimpanzee) ENSRNOP00000010243 Rattus norvegicus (norway rat) XP_798353.2 Strongylocentrotus purpuratus (purple sea urchin) 31864 Trichoplax adhaerens (metazoa) XP_968235.2 Tribolium castaneum (red flour beetle) ENSTNIP00000009465 Tetraodon nigroviridis (green spotted pufferfish) 8006 Thalassiosira pseudonana (marine diatom) ENSTRUP00000018744 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18797 LH21457 ISCW016886 FBgn0229546 FBgn0223842 FBgn0209400 FBgn0187098 FBgn0182914 FBgn0176539 JGI_V11_191117 FBgn0081054 FBgn0158161 FBgn0141891 FBgn0129529 FBgn0112757 FBgn0088331 CPIJ002077 CPIJ002076 BGIBMGA002639 GB10952 AGAP005832 AAEL005181 PHUM407110 GLEAN_01594
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	16495 y1; P{SUPor-P}AsphKG09881 29278 w1118; Mi{ET1}AsphMB12115 6826 y1 w1118; P{PTT-GA}AsphZCL1605
Harvard	- P{XP}Asphd04025 - P{XP}Asphd11430 - PBac{PB}Asphc02390
Kyoto	110795 P{PTT-GA}AsphZCL1605 124108 w1118; P{RS3}AsphCB-6455-3
VDRC	v101484 P{KK109349}VIE-260B v52589 w1118; P{GD4542}v52589/TM3
Genomic Clones ( 2 )
	BACR04O19 BACR29I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT24863 GM05229
Other clones	MIP15930
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT27250 GM19848 GM27985 RE57590 RE62795
Other clones	7016 63913 120890 214221.64 273611 CK01.2D.G6 CK00038 EK125353 EK190843 EK240943 EK300657 EP18148
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034075 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36778
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Asph CG18658 CG8421 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of Asph CG18658 CG8421 was sequence comparison (date:001220). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079033 NM_166140 NM_176185 RefSeq (Proteins) • NP_523757 NP_725525 NP_788365 DDBJ / EMBL / GenBank • AA696179 AAF58063 AAG40806 AAG40807 AAL28453 AAM70947 AAM70948 AAO39637 AC004248 AF289493 AF289494 AW941246 AY060905 BF489148 BT003633 CL528266 CZ467913 CZ473693 CZ473694 CZ477717 Entrez Gene - A searchable database of RefSeq genes. • 36778 UniProtKB/TrEMBL • A4UZJ1 Q7KHE2 Q9GQ82 Q86NU6 Q95S93
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Aspartyl/Asparaginyl beta-hydroxylase (IPR007803) Tetratricopeptide-like helical (IPR011990) Tetratricopeptide repeat-containing (IPR013026)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62502 DroID - A comprehensive database of gene and protein interactions. • FBgn0034075 DRSC - Results from RNAi screens. • FBgn0034075 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034075 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8421-RE;CG8421-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034075 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36778 InParanoid A subset of ortholog calls from InParanoid. • AAEL005181-PA Aedes aegypti (mosquito) AGAP005832-PB Anopheles gambiae (mosquito) BGIBMGA002639-PA Bombyx mori (silk worm) ENSBTAP00000021267 Bos taurus (bovine) CN16304 Caenorhabditis brenneri (small nematode) CE41680 Caenorhabditis elegans (worm) JA08068 Caenorhabditis japonica (nematode) ENSCPOP00000006623 Cavia porcellus (guinea pig) RP43824 Caenorhabditis remanei (nematode) ENSCSAVP00000014978 Ciona savignyi (sedentary tunicate) 168479 Capitella spl (polychaete worm) ENSECAP00000018926 Equus caballus (horse) ENSGALP00000024919 Gallus gallus (chicken) ENSP00000368767 Homo sapiens (human) 144765 Lottia gigantea (owl limpet) 24136 Monocosta brevicollis (choanoflagellate) ENSMODP00000010382 Monodelphis domestica (gray opossum) ENSMMUP00000011501 Macaca mulatta (rhesus monkey) ENSMUSP00000077273 Mus musculus (house mouse) 173452 Nematostella vectensis (starlet anemone) ENSOANP00000008009 Ornithorynchus anatinus (platypus) ENSORLP00000004032 Oryzias latipes (japanese killifish) PHUM001218-PA Pediculus humanus (human body louse) ENSPPYP00000020883 Pongo pygmaeus (orangutan) ENSPTRP00000053155 Pan troglodytes (chimpanzee) ENSRNOP00000010243 Rattus norvegicus (norway rat) XP_798353.2 Strongylocentrotus purpuratus (purple sea urchin) 31864 Trichoplax adhaerens (metazoa) XP_968235.2 Tribolium castaneum (red flour beetle) ENSTNIP00000009465 Tetraodon nigroviridis (green spotted pufferfish) 8006 Thalassiosira pseudonana (marine diatom) ENSTRUP00000018744 Takifugu rubripes (pufferfish Takifugu) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36778 modMine - Data generated by the modENCODE project. • FBgn0034075 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18797 LH21457 ISCW016886 FBgn0229546 FBgn0223842 FBgn0209400 FBgn0187098 FBgn0182914 FBgn0176539 JGI_V11_191117 FBgn0081054 FBgn0158161 FBgn0141891 FBgn0129529 FBgn0112757 FBgn0088331 CPIJ002077 CPIJ002076 BGIBMGA002639 GB10952 AGAP005832 AAEL005181 PHUM407110 GLEAN_01594
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	Asph (Reiter et al., 2007, Quinones-Coello, 2007, Kaltenbach et al., 2007, Crone et al., 2007) BAH/humbug (Dinchuk et al., 2000) CG8421 (Quinones-Coello, 2007, Keleman et al., 2009.8.5, Cammarato et al., 2011) CG18658
Name Synonym	Aspartyl beta-hydroxylase (Cammarato et al., 2011) Aspartyl β-hydroxylase (Dinchuk et al., 2000, Kaltenbach et al., 2007)
Secondary FlyBase IDs
	FBgn0034074
References ( 28 )
Generate a list of	All references relating to this gene ( 28 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 5 ) Review  ( 1 ) Personal communication to FlyBase  ( 7 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Curated genome annotation  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
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