Gene Dmel\MESR4

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\MESR4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

MESR4-RA

FBtr0086946

NM_079051

7489

2171

MESR4-RB

FBtr0113090

NM_001103889

8012

2171

MESR4-RC

FBtr0340219

8006

2169

MESR4-RD

FBtr0340220

7429

2151

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

MESR4-PA

FBpp0086102

241.2

2171

4.74

NP_523775

AAF57837

MESR4-PB

FBpp0112003

241.2

2171

4.74

NP_001097359

ABV53837

MESR4-PC

FBpp0309194

241.0

2169

4.74

MESR4-PD

FBpp0309195

238.9

2151

4.72

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, PHD-type (IPR001965)

Zinc finger, C2H2 (IPR007087)

Zinc finger, C2H2-like (IPR015880)

Zinc finger, FYVE/PHD-type (IPR011011)

Zinc finger, PHD-type, conserved site (IPR019786)

Zinc finger, PHD-finger (IPR019787)

Zinc finger, RING/FYVE/PHD-type (IPR013083)

Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

pole plasm

Comment:maternally deposited

organism

• pole plasm

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

embryonic stage 7 -- 8

organism | ubiquitous

pole cell

embryonic stage 9 -- 10

anterior endoderm

organism | ubiquitous

posterior endoderm

embryonic stage 11 -- 12

anterior midgut primordium

central brain primordium

head mesoderm

organism | ubiquitous

posterior midgut primordium

trunk mesoderm

ventral nerve cord primordium

embryonic stage 13 -- 16

embryonic brain

embryonic/larval midgut

germline cell

gonad

organism | ubiquitous

ventral nerve cord

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		114.45
Larval Midgut		26
Larval Hindgut		49.5
Larval Malpighian Tubules		35.2
Larval Fat Body		33.3
Larval Salivary Gland		73.4
Larval Trachea		67.75
Larval Carcass		51.95
Adult Head		42.6
Adult Eye		41
Adult Brain		86.4
Adult Thoracic-Abdominal Ganglion		63.8
Adult Crop		55.3
Adult Midgut		28.6
Adult Hindgut		46.5
Adult Malpighian Tubules		25
Adult Fat Body		22.5
Adult Salivary Gland		61.4
Adult Heart		35.625
Adult VirginFemale Spermatheca		19.4
Adult InseminatedFemale Spermatheca		23.8
Adult Ovary		206.6
Adult Testis		44.6
Adult Male Accessory Gland		59
Adult Carcass		52.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	42.6
NA	Eye	41
NA	Brain	86.4
114.45	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	63.8
NA	Crop	55.3
no informative data	Midgut	no informative data
49.5	Hindgut	46.5
35.2	Malpighian Tubules	no informative data
no informative data	Fat Body	no informative data
no informative data	Salivary Gland	no informative data
NA	Heart	35.625
67.75	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	206.6
NA	Testis	44.6
NA	Male Accessory Gland	59
51.95	Carcass	52.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(43)
ovary, mated 4-day female		(44)
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(43)
ovary, mated 4-day female		(44)
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		22
central nervous system, pupae P8		13
head, virgin 1-day female		7
head, virgin 4-day female		7
head, virgin 20-day female		7
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		6
head, mated 1-day male		10
head, mated 4-day male		11
head, mated 20-day male		12
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		7
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		3
fat body, white prepupae		7
fat body, pupae P8		3
carcass, larvae L3 wandering		14
carcass, 1-day adult		5
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		43
ovary, mated 4-day female		44
testis, mated 4-day male		2
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(40)
embryo 02-04hr		(32)
embryo 04-06hr		(28)
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(40)
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		40
embryo 02-04hr		32
embryo 04-06hr		28
embryo 06-08hr		20
embryo 08-10hr		15
embryo 10-12hr		6
embryo 12-14hr		11
embryo 14-16hr		9
embryo 16-18hr		9
embryo 18-20hr		7
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		10
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		8
larva L3 puffstage 7-9		11
white prepupae new		11
white prepupae 12hr		10
white prepupae 24hr		11
pupae 2d postWPP		8
pupae 3d postWPP		5
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		7
adult male 30day		6
adult female 01day		12
adult female 05day		24
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		(27)
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		19
Schneider line 2 Sg4		6
embryonic 1182-4H		14
embryonic GM2		11
embryonic Kc167		11
embryonic S1		11
embryonic S3		10
leg disc CME L1		11
wing disc CME-W2		12
wing disc ML-DmD8		14
wing disc ML-DmD9		10
wing disc ML-DmD16-c3		19
wing disc ML-DmD21		1
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		4
mixed discs ML-DmD4-c1		6
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		22
tumorous blood cells mbn2		21
ovary fGS/OSS		27
ovary OSC		20
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		(27)
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		(33)
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(28)
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		(33)
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		7
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		27
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		33
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for MESR4

BDGP expression data

•

LP07188

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034240

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4903-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

MESR4^CB04813

partially lethal - majority die, with Scer\GAL4^ap-md544

MESR4^EP988

viable, with Scer\GAL4[-]:

MESR4^EP386

viable, with Scer\GAL4^pnr-MD237

MESR4^GD11465

Other Phenotypes

Allele

mitotic cell cycle defective

MESR4^EP386

visible, with Scer\GAL4^pnr-MD237

MESR4^GD11465

visible, with Scer\GAL4^sca-537.4

Phenotype manifest in

Allele

adult abdomen & macrochaeta, with Scer\GAL4^sca-537.4

dorsocentral bristle, with Scer\GAL4^sca-537.4

MESR4^EP988

scutellar bristle, with Scer\GAL4^sca-537.4

MESR4^EP988

thecogen cell | ectopic, with Scer\GAL4^sca-537.4

MESR4^EP386

tormogen cell, with Scer\GAL4^sca-537.4

MESR4^EP386

trichogen cell, with Scer\GAL4^pnr-MD237

MESR4^GD11465

trichogen cell, with Scer\GAL4^sca-537.4

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

MESR4^EP386

wing, with Scer\GAL4^ap-md544

MESR4^EP988

Classical Alleles ( 15 )

For All Classical Alleles Show

Allele of MESR4	Mutagen	Stocks	Known lesion
MESR4^5-HA-1709		1	--
MESR4^5-HA-2854		1	--
MESR4^5-SZ-4062		1	--
MESR4^d09351		1	--
MESR4^e01745	piggyBac transposase	1	--
MESR4^EY03179		1	--
MESR4^G12997	P-element activity	1	--
MESR4^CB04813	P-element activity	0	--
MESR4^EP2156		0	--
MESR4^EP2281		0	--
MESR4^EP2393		0	--
MESR4^EP386	P-element activity	0	Yes
MESR4^EP988	P-element activity	0	Yes
MESR4^EY12914		0	--
MESR4^NP6338		0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of MESR4	Mutagen	Stocks	Known lesion
MESR4^GD11465	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
MESR4^GL00462	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
MESR4^NIG.4903R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11465}

P{NIG.4903R}

P{TRiP.GL00462}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}MESR4^EY03179

P{EPgy2}MESR4^EY12914

P{XP}MESR4^d09351

miscellaneous insertions

P{(loxP.en.8)w}17A

P{PTT-GB}MESR4^CB04813

P{RS5}MESR4^5-HA-1709

P{RS5}MESR4^5-HA-2854

P{RS5}MESR4^5-SZ-4062

PBac{RB}MESR4^e01745

insertion of enhancer trap binary system

P{GawB}MESR4^NP6338

Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )

Terms Based on Experimental Evidence ( 3 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

cellular response to hypoxia

inferred from mutant phenotype

(Lee et al., 2008)

negative regulation of Ras protein signal transduction

inferred from genetic interaction with ksr

inferred from genetic interaction with Ras85D

regulation of response to DNA damage stimulus

inferred from genetic interaction with lok

(Park and Song, 2008)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

nucleic acid binding

inferred from electronic annotation with InterPro:IPR013087

(FlyBase Curators et al., 2004-)

zinc ion binding

inferred from electronic annotation with InterPro:IPR001965, InterPro:IPR007087, InterPro:IPR015880

(FlyBase Curators et al., 2004-)

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

MESR4 - fs(1)h

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

MESR4 allele

Gene

References

MESR4^EP386

(Abdelilah-Seyfried et al., 2000)

ksr::tor

(Abdelilah-Seyfried et al., 2000)

Ras85D

Syx1A

(Pena-Rangel et al., 2002)

Pvr

(Zhu et al., 2005)

unspecified

Act42A

aop

Bacc

EP704

EP1244

Etl1

kis

ksr

Ppt1

(Buff et al., 2007)

External Data

y¹ w^67c23; P{EPgy2}MESR4^EY03179

BioGRID - A database of protein and genetic interactions

•

62675

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0034240

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

36986

Orthologs

OrthoDB Orthologs (31) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG618C6S

Dipteran inclusive ortholog search

EOG647G32

Insect inclusive ortholog search

EOG6WDC6Q

Arthropod inclusive ortholog search

EOG62Z352

Metazoa inclusive ortholog search

EOG6HXHSV

Orthologs in Drosophila Species (EOG618C6S)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18514

Drosophila persimilis

Dper\GL11465

Drosophila willistoni

Dwil\GK21900

Drosophila virilis

Dvir\GJ21428

Drosophila mojavensis

Dmoj\GI18359

Drosophila grimshawi

Dgri\GH20921

Orthologs in non-Drosophila Dipterans (EOG647G32)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009391

Anopheles gambiae

Malaria mosquito

Agam\AGAP011875

Orthologs in non-Dipteran Insects (EOG6WDC6Q)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC100576321

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008068

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH10146

Atta cephalotes

Leafcutter ant

Acep\ACEP20698

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17998

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO26352

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL16149

Linepithema humile

Argentine ant

Lhum\LH25381

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15609

Solenopsis invicta

Red fire ant

Sinv\SINV17990

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI52571

Bombyx mori

Silkmoth

Bmor\BGIBMGA002817

Pediculus humanus

Human body louse

Phum\PHUM012910

Tribolium castaneum

Red flour beetle

Tcas\TC012247

Tribolium castaneum

Red flour beetle

Tcas\TC001850

Orthologs in non-Insect Arthropods (EOG62Z352)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_46900

Orthologs in non-Arthropod Metazoa (EOG6HXHSV)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\Y43H11AL.1

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18514

Drosophila persimilis

Dper\GL11465

Drosophila willistoni

Dwil\GK21900

Drosophila virilis

Dvir\GJ21428

Drosophila mojavensis

Dmoj\GI18359

Drosophila grimshawi

Dgri\GH20921

Stocks & Reagents

Stocks Listed in FlyBase ( 10 )

Bloomington

28392

y¹ w^*; P{EP}MESR4^G12997

20148

35618

y¹ sc^* v¹; P{TRiP.GL00462}attP2

125357

w¹¹¹⁸; P{RS5}MESR4^5-HA-1709

125707

w¹¹¹⁸; P{RS5}MESR4^5-HA-2854

126319

w¹¹¹⁸; P{RS5}MESR4^5-SZ-4062

VDRC

v21974

w¹¹¹⁸; P{GD11465}v21974

v21976

w¹¹¹⁸; P{GD11465}v21976

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 33 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

IP14545

Other clones

LD04271

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GM09956

Other clones

RNAi & Array Information