FlyBase Gene Report: Dmel\Atg7

General Information

Symbol

Dmel\Atg7

Species

D. melanogaster

Name

Autophagy-related 7

Annotation Symbol

CG5489

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034366

Gene Model Status

Stock Availability

12 publicly available

Gene Summary

Autophagy-related 7 (Atg7) encodes an E1-type ligase for the autophagic ubiquitin-like proteins encoded by Atg8a and Atg12. Its roles include autophagosome formation, maintaining neuromuscular function and normal lifespan. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dAtg7, autophagy-related gene 7, autophagy-related protein 7, Autophagy-specific gene 7, atg-7

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:18,620,466..18,625,044 [-]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18620466..18625044

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables Atg8 activating enzyme activity

inferred from direct assay

(Chang et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables Atg12 activating enzyme activity

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

enables Atg8 activating enzyme activity

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

Biological Process (21 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Wen et al., 2017, Xu et al., 2015)

involved_in cellular response to starvation

inferred from mutant phenotype

(Hou et al., 2008)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Juhász et al., 2007)

involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Juhász et al., 2007)

involved_in macroautophagy

inferred from mutant phenotype

(Juhász et al., 2007, Scott et al., 2004)

involved_in oogenesis

inferred from mutant phenotype

(Hou et al., 2008)

involved_in positive regulation of autophagy of mitochondrion

inferred from mutant phenotype

(assigned by UniProt )

(Vincow et al., 2013)

involved_in positive regulation of macroautophagy

inferred from mutant phenotype

(Hou et al., 2008)

involved_in protein lipidation

inferred from mutant phenotype

(Chang et al., 2013)

involved_in protein modification by small protein conjugation

inferred from direct assay

(Chang et al., 2013)

involved_in regulation of autophagy

inferred from mutant phenotype

(Pircs et al., 2012, Barth et al., 2011)

involved_in regulation of defense response to virus

inferred from mutant phenotype

(Shelly et al., 2009)

NOT involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Chang et al., 2013)

Terms Based on Predictions or Assertions (11 terms)

CV Term

Evidence

References

involved_in autophagosome assembly

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

involved_in autophagy of mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

involved_in autophagy

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

involved_in C-terminal protein lipidation

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in cellular response to nitrogen starvation

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in late nucleophagy

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in negative regulation of apoptotic process

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

involved_in negative regulation of cell death

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

involved_in piecemeal microautophagy of the nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein modification by small protein conjugation

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000102039

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of hemopoiesis

inferred from sequence or structural similarity with UniProtKB:Q9D906

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000102040

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in phagophore assembly site

inferred from biological aspect of ancestor with PANTHER:PTN000102341

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in phagophore assembly site

inferred from sequence or structural similarity with SGD:S000001214

(FlyBase Curators, 2008--)

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES

ATG8/12 ACTIVATING ENZYMES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Autophagy-related 7 (Atg7) encodes an E1-type ligase for the autophagic ubiquitin-like proteins encoded by Atg8a and Atg12. Its roles include autophagosome formation, maintaining neuromuscular function and normal lifespan. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES -: The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).
ATG8/12 ACTIVATING ENZYMES -: ATG8/12 activating enzymes are 'non-canonical' E1 enzymes that form obligate homodimers and activate the ubiquitin-like proteins ATG8 and ATG12 for subsequent steps along their respective conjugation cascades. (Adapted from PMID:28234446.)

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Atg7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0086712

2395

684

FBtr0086711

1873

510

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0085891

76.8

684

6.00

FBpp0085890

56.9

510

5.79

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg7 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Lee et al., 2003)

Additional Descriptive Data

The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.

(Lee et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Atg7 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Atg7

View exonic expression by tissue for Dmel\Atg7

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034366

DRscDB

37141/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034366

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5489-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5489

Flygut - An atlas of the Drosophila adult midgut

FBgn0034366

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Atg7

Transgenic constructs containing regulatory region of Atg7

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene partially disrupted in

(Juhász et al., 2007)

(Juhász et al., 2007)

Df(2R)CG5335^d30

(Juhász et al., 2007)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Atg7^GD11671

viable, with Scer\GAL4^Mef2.PR

Atg7^GD11671

viable, with Scer\GAL4^pnr-MD237

Atg7^GD11671

viable, with Scer\GAL4^tin.CΔ4

Atg7^GD11671

viable (with Atg7^d14)

Atg7^d77

viable (with Atg7^d77)

Atg7^d14

Other Phenotypes

Allele

abnormal immune response | adult stage, with Scer\GAL4^hs.PB

Atg7^dsRNA.UAS

abnormal immune response | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

Atg7^dsRNA.UAS

abnormal memory | progressive (with Atg7^d14)

Atg7^d77

abnormal memory | progressive (with Atg7^d77)

Atg7^d14

abnormal neuroanatomy (with Atg7^d14)

Atg7^d77

abnormal neuroanatomy (with Atg7^d77)

Atg7^d14

abnormal size | adult stage (with Atg7^d14)

Atg7^d77

abnormal size | adult stage (with Atg7^d77)

Atg7^d14

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

Atg7^JF02787

abnormal stress response (with Atg7^d14)

Atg7^d77

abnormal stress response (with Atg7^d77)

Atg7^d14

chemical sensitive (with Atg7^d14)

Atg7^d77

chemical sensitive (with Atg7^d77)

Atg7^d14

decreased cell death | adult stage | nutrition conditional (with Atg7^d14)

Atg7^d77

decreased cell death | adult stage | nutrition conditional (with Atg7^d77)

Atg7^d14

decreased cell death | prepupal stage (with Atg7^d14)

Atg7^d77

decreased cell death | prepupal stage (with Atg7^d77)

Atg7^d14

decreased cell death | somatic clone

Atg7^d4

decreased cell number | dominant | larval stage

Atg7^d77

increased cell death | adult stage (with Atg7^d14)

Atg7^d77

increased cell death | adult stage (with Atg7^d77)

Atg7^d14

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

Atg7^HMS01358

long lived, with Scer\GAL4^Appl.PU

Atg7^UAS.cCa

long lived, with Scer\GAL4^hs.PU

Atg7^UAS.cCa

short lived, with Scer\GAL4^hs.PU

Atg7^GD11671

short lived (with Atg7^d14)

Atg7^d77

short lived (with Atg7^d77)

Atg7^d14

visible, with Scer\GAL4^GMR.PU

Atg7^GD11671

Phenotype manifest in

Allele

adult brain (with Atg7^d14)

Atg7^d77

adult brain (with Atg7^d77)

Atg7^d14

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

Atg7^HMS01358

adult gut (with Atg7^d14)

Atg7^d77

adult gut (with Atg7^d77)

Atg7^d14

adult midgut epithelium, with Scer\GAL4^esg.PU

Atg7^HMS01358

autophagic vacuole & larval midgut (with Atg7^d14)

Atg7^d77

autophagic vacuole & larval midgut (with Atg7^d77)

Atg7^d14

autophagosome, with Scer\GAL4^unspecified

Atg7^GD11671

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg7^HMS01358

Atg7^d14

egg chamber | nutrition conditional (with Atg7^d14)

Atg7^d77

egg chamber | nutrition conditional (with Atg7^d77)

Atg7^d14

egg chamber | somatic clone

Atg7^d4

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

Atg7^JF02787

embryonic/larval fat body | third instar larval stage (with Atg7^d14)

Atg7^d77

embryonic/larval fat body | third instar larval stage (with Atg7^d77)

Atg7^d14

embryonic/larval midgut | prepupal stage (with Atg7^d14)

Atg7^d77

embryonic/larval midgut | prepupal stage (with Atg7^d77)

Atg7^d14

eye, with Scer\GAL4^GMR.PU

Atg7^GD11671

female germline cyst | nutrition conditional (with Atg7^d14)

Atg7^d77

female germline cyst | nutrition conditional (with Atg7^d77)

Atg7^d14

gamma Kenyon cell (with Atg7^d14)

Atg7^d77

gamma Kenyon cell (with Atg7^d77)

Atg7^d14

germarium | somatic clone

Atg7^d4

germarium region 2 | nutrition conditional (with Atg7^d14)

Atg7^d77

germarium region 2 | nutrition conditional (with Atg7^d77)

Atg7^d14

intestinal stem cell | chemical conditional, with Scer\GAL4^NP0001

Atg7^dsRNA.UAS

intestinal stem cell | chemical conditional, with Scer\GAL4^NP5130

Atg7^dsRNA.UAS

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg7^HMS01358

major mitochondrial derivative, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Atg7^HMS01358

major mitochondrial derivative (with Atg7^d14)

Atg7^d77

major mitochondrial derivative (with Atg7^d77)

Atg7^d14

ommatidium, with Scer\GAL4^GMR.PU

Atg7^GD11671

ommatidium | adult stage | light conditional, with Scer\GAL4^GMR.PU

Atg7^NIG.5489R

prothoracic gland | third instar larval stage, with Scer\GAL4^phm.PO

Atg7^JF02787

Atg7^d77

rhabdomere | adult stage | decreased number | light conditional, with Scer\GAL4^GMR.PU

Atg7^NIG.5489R

RP2 motor neuron | larval stage

Atg7^d77

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

3

1 of 15

No

No

1 of 15

No

No

1

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (10)

12 of 13

Yes

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (9)

3 of 12

Yes

Yes

Xtro\LOC100495145

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (8)

3 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (5)

13 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (1)

9 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (7)

13 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (1)

11 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903IE )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00005706

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502T2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY010920

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11392

Mayetiola destructor

Hessian fly

Mdes\Mdes007464

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009814

Anopheles gambiae

Malaria mosquito

Agam\AGAP010303

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02KH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001467

Danaus plexippus

Monarch butterfly

Dple\DPOGS208037-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL004052

Apis florea

Little honeybee

Aflo\2542875524

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002788

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000710

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM397480

Rhodnius prolixus

Kissing bug

Rpro\RPRC012046

Rhodnius prolixus

Kissing bug

Rpro\RPRC012048

Cimex lectularius

Bed bug

Clec\CLEC000439

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008937

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_17566

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04460

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02HT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR007013

Ixodes scapularis

Black-legged tick

Isca\ISCW008151

Stegodyphus mimosarum

African social velvet spider

Smim\KFM67470.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g02140

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302357

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0628 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004841

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

ameliorates myotonic dystrophy type 1

modeled by Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

ameliorates Parkinson's disease 1

modeled by

(Yan et al., 2019)

modeled by Hsap\SNCA^UAS.cFa

(Yan et al., 2019)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^695.UAS

(Zhuang et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATG7; autophagy related 7

14 of 15

SPINOCEREBELLAR ATAXIA, AUTOSOMAL RECESSIVE 31; SCAR31

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

RNA-protein

Physical Interaction

Assay

References

pull down, western blot, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Rojas-Ríos et al., 2015)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Sim et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Nandi et al., 2014)

suppressible

(Tang et al., 2013)

suppressible

(Sim et al., 2019)

enhanceable

(Sim et al., 2019)

suppressible

(Kim et al., 2010)

suppressible

(Shinoda et al., 2019)

enhanceable

(Kwon et al., 2013)

suppressible

(Chen et al., 2012)

suppressible

(Yan et al., 2019)

enhanceable

(Dodson et al., 2014)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0034366

MIST (genetic) - An integrated Molecular Interaction Database

Atg7

MIST (protein-protein) - An integrated Molecular Interaction Database

Atg7

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-86

Cytogenetic map

Sequence location

2R:18,620,466..18,625,044 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55E2-55E2

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

19257

w¹¹¹⁸; P{XP}Atg7^d06996/CyO

27707

y¹ v¹; P{TRiP.JF02787}attP2

80821

y¹, v¹; P{TKO.GS04628}attP40

17635

y¹ w^67c23; P{EPgy2}Sec6^EY10058

34369

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01358}attP2/TM3, Sb¹

79288

y¹ w^*; TI{CRIMIC.TG4.0}Atg7^{CR00724-TG4.0} CG5335^{CR00724-TG4.0-X}

86389

y¹ w^*; TI{CRIMIC.TG4.2}CG5335^{CR01440-TG4.2} Atg7^{CR01440-TG4.2-X}

114560

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{XP}Atg7^d06996 /CyO y⁺

v27432

w¹¹¹⁸ P{GD11671}v27432

v45558

w¹¹¹⁸; P{GD11671}v45558

v45560

w¹¹¹⁸; P{GD11671}v45560/TM3

v45561

w¹¹¹⁸; P{GD11671}v45561/TM3

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (46)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE27292

Other clones

DPiM_RE27292

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034366

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034366

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37141

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Atg7 CG5489

(Scott et al., 2004)

Source for database merge of

Additional comments

Other Comments

Atg7 is required for the normal clearance of the paternal mitochondrial derivatives in developing embryos.

(Politi et al., 2014)

Atg7 mutants are viable and no major morphological defects are seen during metamorphosis. The mutant adults are hypersensitive to stress and have a reduced life span.

(Juhász et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37141

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034366

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034366

DRscDB

37141/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034366

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5489

Flygut - An atlas of the Drosophila adult midgut

FBgn0034366

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37141

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010974

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5489

MARRVEL_MODEL

37141

modMine - A data warehouse for the modENCODE project

FBgn0034366

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0034366

DRSC - Results frm RNAi screens

FBgn0034366

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5489-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034366

FlyMine - An integrated database for Drosophila genomics

FBgn0034366

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Atg7

MIST (protein-protein) - An integrated Molecular Interaction Database

Atg7

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

ATG-7

(Yadav and Srikrishna, 2019)

ATG7

(Song et al., 2020, Yang et al., 2020, Kourtis and Tavernarakis, 2009)

Atg7

(Bedont et al., 2021, Jacomin et al., 2021, Schroeder et al., 2021, Yu and Hyun, 2021, Ahmed and Vogel, 2020, Barthez et al., 2020, Cunningham et al., 2020, Donde et al., 2020, Jacomin et al., 2020, Lu et al., 2020, Luo et al., 2020, Nagai et al., 2020, Nakajima et al., 2020, Sênos Demarco et al., 2020, Xu et al., 2020, Zhuang et al., 2020, Bhattacharjee et al., 2019, Guo et al., 2019, Jacomin et al., 2019, Johnson et al., 2019, Khezri and Rusten, 2019, Meltzer et al., 2019, Schroeder et al., 2019, Sênos Demarco et al., 2019, Sim et al., 2019, Vincow et al., 2019, Xu et al., 2019, Yan et al., 2019, Zhang et al., 2019, Billes et al., 2018, Chakraborty et al., 2018, Gene Disruption Project members, 2018-, Kuo et al., 2018, Lund et al., 2018, O'Keefe and Denton, 2018, Richardson and Portela, 2018, Swevers et al., 2018, Thomas et al., 2018, Bali and Shravage, 2017, Galluzzi et al., 2017, Kim et al., 2017, Kim et al., 2017, Lei et al., 2017, Lo Piccolo et al., 2017, Manent et al., 2017, Nagy et al., 2017, Péan et al., 2017, Schopf and Huber, 2017, Transgenic RNAi Project members, 2017-, Tusco et al., 2017, Wen et al., 2017, Danielsen et al., 2016, Etchegaray et al., 2016, Gupta et al., 2016, Kuleesha et al., 2016, Lee et al., 2016, Lőrincz et al., 2016, Mao et al., 2016, Varga et al., 2016, Zheng et al., 2016, Bargiela et al., 2015, Carnes et al., 2015, Denton et al., 2015, Johnson et al., 2015, Kim et al., 2015, Nagy et al., 2015, Tian et al., 2015, Xu et al., 2015, Zhang and Baehrecke, 2015, Zirin et al., 2015, Banreti et al., 2014, Burke et al., 2014, DeVorkin et al., 2014, Issman-Zecharya and Schuldiner, 2014, Liu et al., 2014, Moy et al., 2014, Mulakkal et al., 2014, Nagy et al., 2014, Nandi et al., 2014, Takáts et al., 2014, Chang et al., 2013, Denton et al., 2013, Gupta et al., 2013, Lin et al., 2013, Moy and Cherry, 2013, Shin et al., 2013, Shravage et al., 2013, Takáts et al., 2013, Tang et al., 2013, Velentzas et al., 2013, Vincow et al., 2013, Wen et al., 2013, Xiong and Bellen, 2013, Yacobi-Sharon et al., 2013, Chen et al., 2012, Eleftherianos and Castillo, 2012, Érdi et al., 2012, Garcia et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Pircs et al., 2012, Wang et al., 2012, Barth et al., 2011, Bartlett et al., 2011, Du et al., 2011, Lee et al., 2011, Tang et al., 2011, Bakhrat et al., 2010, Chang and Neufeld, 2010, Chi et al., 2010, Demontis and Perrimon, 2010, Filimonenko et al., 2010, Kadandale et al., 2010, Kim et al., 2010, LaFever et al., 2010, Wasbrough et al., 2010, Zirin and Perrimon, 2010, Ren et al., 2009, Shelly et al., 2009, Chen et al., 2008, Hou et al., 2008, Liu and Lehmann, 2008, Juhasz et al., 2007, Juhász et al., 2007, Zeitouni et al., 2007)

CG5489

(Tsou et al., 2015, Fukuoh et al., 2014, Érdi et al., 2012, Ni et al., 2010.12.1, Perkins et al., 2009, Lee et al., 2003)

atg-7

(Nezis, 2012, Midorikawa et al., 2010)

dAtg7

(Chattopadhyay and Thirumurugan, 2020, Wang et al., 2020, Li et al., 2016, Velentzas et al., 2013)

Name Synonyms

Autophagy-related 7

(Carnes et al., 2015)

Autophagy-specific gene 7

(Scott et al., 2004, Scott et al., 2004)

autophagy-related gene 7

(Chen et al., 2012, Minois et al., 2012)

autophagy-related protein 7

(Nezis, 2012, Midorikawa et al., 2010)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (218)

Report Sections