General Information
Symbol
Dmel\Atg7
Species
D. melanogaster
Name
Autophagy-related 7
Annotation Symbol
CG5489
Feature Type
FlyBase ID
FBgn0034366
Gene Model Status
Stock Availability
Enzyme Name (EC)
E1 ubiquitin-activating enzyme (6.2.1.45)
Gene Snapshot
Autophagy-related 7 is an E1-type ligase for the autophagic ubiquitin-like proteins Atg8a and Atg12. Its roles include autophagosome formation, maintaining neuromuscular function and normal lifespan. [Date last reviewed: 2016-06-30]
Also Known As
atg-7
Genomic Location
Cytogenetic map
Sequence location
2R:18,620,466..18,625,044 [-]
Recombination map
2-86
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Catalytic Activity (EC)
Experimental Evidence
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine (6.2.1.45)
Predictions / Assertions
-
Summaries
Gene Group Membership
UBIQUITIN ACTIVATING ENZYMES -
E1 enzymes use ATP to activate the carboxyl terminus of ubiquitin or ubiquitin-like proteins forming a high-energy AMP conjugate, which is then subsequently transferred to the E1 active site cysteine to form a thioester intermediate. In many cases, the ubiquitin/ubiquitin-like molecule is then transferred to an E2 active site cysteine via trans-thioesterification. (Adapted from PMID:23800009).
AUTOPHAGY-RELATED GENES -
The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Atg7 or the JBrowse view of Dmel\Atg7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086712
2395
684
FBtr0086711
1873
510
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085891
76.8
684
6.00
FBpp0085890
56.9
510
5.79
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg7 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (25 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000001214
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000001214
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
Biological Process (20 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (11 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000001214
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000001214
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000001214
inferred from sequence or structural similarity with UniProtKB:Q9D906
(assigned by ParkinsonsUK-UCL )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000102039
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000102341
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000001214
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Atg7 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Allele of Atg7
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    --
    Other relevant insertions
    insertion of mobile activating element
    Name
    Expression Data
    Transgenic Constructs ( 6 )
    Deletions and Duplications ( 3 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    autophagic vacuole & larval midgut (with Atg7d14)
    autophagic vacuole & larval midgut (with Atg7d77)
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    14 of 15
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    13 of 15
    Yes
    Yes
    Rattus norvegicus (Norway rat) (1)
    12 of 13
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (1)
    2 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (1)
    2 of 15
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (1)
    12 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (1)
    9 of 9
    Yes
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (1)
    14 of 15
    Yes
    Yes
    Schizosaccharomyces pombe (Fission yeast) (1)
    12 of 12
    Yes
    Yes
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903IE )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502T2 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles gambiae
    Malaria mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02KH )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Danaus plexippus
    Monarch butterfly
    Heliconius melpomene
    Postman butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Dendroctonus ponderosae
    Mountain pine beetle
    Dendroctonus ponderosae
    Mountain pine beetle
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Rhodnius prolixus
    Kissing bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02HT )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Ixodes scapularis
    Black-legged tick
    Stegodyphus mimosarum
    African social velvet spider
    Tetranychus urticae
    Two-spotted spider mite
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0628 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Ciona intestinalis
    Vase tunicate
    Gallus gallus
    Domestic chicken
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 2 )
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please look at the Interaction Group reports for full details of the physical interactions
      protein-protein
      Interacting group
      Assay
      References
      RNA-protein
      Interacting group
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2R
      Recombination map
      2-86
      Cytogenetic map
      Sequence location
      2R:18,620,466..18,625,044 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      55E2-55E2
      Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)0370903709
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (11)
      Genomic Clones (17)
      cDNA Clones (46)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: Atg7 CG5489
      Source for database merge of
      Additional comments
      Other Comments
      Atg7 is required for the normal clearance of the paternal mitochondrial derivatives in developing embryos.
      Atg7 mutants are viable and no major morphological defects are seen during metamorphosis. The mutant adults are hypersensitive to stress and have a reduced life span.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 38 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      Atg7
      (Chakraborty et al., 2018, Gene Disruption Project members, 2018-, Kuo et al., 2018, O'Keefe and Denton, 2018, Richardson and Portela, 2018, Swevers et al., 2018, Galluzzi et al., 2017, Kim et al., 2017, Kim et al., 2017, Lei et al., 2017, Lo Piccolo et al., 2017, Manent et al., 2017, Nagy et al., 2017, Péan et al., 2017, Schopf and Huber, 2017, Tusco et al., 2017, Wen et al., 2017, Danielsen et al., 2016, Lee et al., 2016, Lőrincz et al., 2016, Mao et al., 2016, Varga et al., 2016, Zheng et al., 2016, Bargiela et al., 2015, Carnes et al., 2015, Denton et al., 2015, Johnson et al., 2015, Kim et al., 2015, Nagy et al., 2015, Tian et al., 2015, Xu et al., 2015, Zhang and Baehrecke, 2015, Zirin et al., 2015, Banreti et al., 2014, Burke et al., 2014, DeVorkin et al., 2014, Issman-Zecharya and Schuldiner, 2014, Liu et al., 2014, Moy et al., 2014, Mulakkal et al., 2014, Nagy et al., 2014, Nandi et al., 2014, Takáts et al., 2014, Chang et al., 2013, Denton et al., 2013, Gupta et al., 2013, Lin et al., 2013, Moy and Cherry, 2013, Shin et al., 2013, Shravage et al., 2013, Takáts et al., 2013, Tang et al., 2013, Velentzas et al., 2013, Vincow et al., 2013, Wen et al., 2013, Xiong and Bellen, 2013, Yacobi-Sharon et al., 2013, Chen et al., 2012, Eleftherianos and Castillo, 2012, Érdi et al., 2012, Garcia et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Pircs et al., 2012, Wang et al., 2012, Barth et al., 2011, Bartlett et al., 2011, Du et al., 2011, Lee et al., 2011, Tang et al., 2011, Bakhrat et al., 2010, Chi et al., 2010, Demontis and Perrimon, 2010, Filimonenko et al., 2010, Kadandale et al., 2010, Kim et al., 2010, LaFever et al., 2010, Wasbrough et al., 2010, Zirin and Perrimon, 2010, Ren et al., 2009, Shelly et al., 2009, Chen et al., 2008, Hou et al., 2008, Liu and Lehmann, 2008, Juhasz et al., 2007, Juhász et al., 2007, Zeitouni et al., 2007)
      Name Synonyms
      Autophagy-related 7
      Autophagy-specific gene 7
      autophagy-related gene 7
      autophagy-related protein 7
      Secondary FlyBase IDs
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (159)