FB2020_06, released Dec 22, 2020

Gene: Dmel\Mctp

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General Information
Symbol
Dmel\Mctp
Species
D. melanogaster
Name
Multiple C2 domain and transmembrane region protein
Annotation Symbol
CG15078
Feature Type
protein_coding_gene
FlyBase ID
FBgn0034389
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
Multiple C2 domain and transmembrane region protein (Mctp) encodes a regulator of both neuronal synaptic plasticity and neurotransmitter secretion. [Date last reviewed: 2019-09-12]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
55F1-55F2
Sequence location
2R:18,761,758..18,774,824 [+]
Recombination map

2-86

RefSeq locus
NT_033778 REGION:18761758..18774824
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables calcium ion binding
inferred from direct assay
(assigned by UniProt )
(Genç et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables calcium ion binding
inferred from sequence or structural similarity with HGNC:26183
(Shin et al., 2005)
inferred from biological aspect of ancestor with PANTHER:PTN002473508
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in regulation of neuronal synaptic plasticity
inferred from mutant phenotype
(assigned by UniProt )
(Genç et al., 2017)
involved_in regulation of neurotransmitter secretion
inferred from mutant phenotype
(assigned by UniProt )
(Genç et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of neurotransmitter secretion
inferred from biological aspect of ancestor with PANTHER:PTN000413636
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in endoplasmic reticulum membrane
inferred from direct assay
(assigned by UniProt )
(Genç et al., 2017)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in integral component of membrane
inferred from sequence or structural similarity with HGNC:26183
(Shin et al., 2005)
inferred from biological aspect of ancestor with PANTHER:PTN002473508
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in synaptic vesicle membrane
inferred from biological aspect of ancestor with PANTHER:PTN000413636
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Calcium sensor which is essential for the stabilization of normal baseline neurotransmitter release and for the induction and long-term maintenance of presynaptic homeostatic plasticity (PubMed:28485711).
(UniProt, A1ZBD6)
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
5

Please see the JBrowse view of Dmel\Mctp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.56

Stop-codon suppression (UAG) postulated; FBrf0216884

gene_with_stop_codon_read_through ; SO:0000697

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(Jungreis et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mctp-RA
FBtr0110970
NM_137528
4066
893
Mctp-RB
FBtr0110971
NM_001043095
2824
596
Mctp-RD
FBtr0330004
NM_001274149
4123
982
Mctp-RE
FBtr0330005
NM_001043094
4015
954
Mctp-RF
FBtr0345950
NM_001299661
4223
893
Mctp-RG
FBtr0346955
NM_001316428
4123
912
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Mctp-PA
FBpp0110270
101.1
893
7.24
NP_611372
AAO41353
Mctp-PB
FBpp0110271
69.1
596
7.52
NP_001036560
AAF57640
Mctp-PD
FBpp0303039
110.6
982
8.26
NP_001261078
AGB93610
Mctp-PE
FBpp0303040
108.1
954
8.22
NP_001036559
ABI31105
Mctp-PF
FBpp0311864
101.1
893
7.24
NP_001286590
AHN56385
Mctp-PG
FBpp0312419
103.5
912
7.67
NP_001303357
ALI30186
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

893 aa isoforms: Mctp-PA, Mctp-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mctp using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism

Comment: rapidly degraded

(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in endoplasmic reticulum membrane
inferred from direct assay
(assigned by UniProt )
(Genç et al., 2017)
Expression Deduced from Reporters
Reporter: P{GT1}MctpBG01140b
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
eo support cell of olfactory sensory organ | subset
(Tunstall et al., 2012)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mctp in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mctp
Transgenic constructs containing regulatory region of Mctp
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MCTP1
13 of 15
Yes
Yes
Hsap\MCTP2
11 of 15
No
Yes
Hsap\ESYT1
1 of 15
No
No
Hsap\ESYT2
1 of 15
No
No
Hsap\ESYT3
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Mctp1
13 of 15
Yes
Yes
Mmus\Mctp2
11 of 15
No
Yes
Mmus\Esyt1
1 of 15
No
No
Mmus\Esyt2
1 of 15
No
No
Mmus\Esyt3
1 of 15
No
No
Rattus norvegicus (Norway rat) (2)
Rnor\Mctp1
8 of 13
Yes
Yes
Rnor\Mctp2
8 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\mctp1
8 of 12
Yes
Yes
Xtro\mctp2
7 of 12
No
Yes
Xtro\esyt3
1 of 12
No
No
Danio rerio (Zebrafish) (7)
Drer\mctp1b
13 of 15
Yes
Yes
Drer\mctp1a
8 of 15
No
Yes
Drer\mctp2a
8 of 15
No
Yes
Drer\mctp2b
6 of 15
No
Yes
Drer\esyt2a
1 of 15
No
No
Drer\esyt2b
1 of 15
No
No
Drer\esyt3
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\mctp-1
12 of 15
Yes
Yes
Cele\esyt-2
1 of 15
No
No
Cele\snt-2
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (17)
Atha\AT3G61300
7 of 9
Yes
Yes
Atha\AT1G04150
5 of 9
No
Yes
Atha\AT1G22610
5 of 9
No
Yes
Atha\AT1G51570
5 of 9
No
Yes
Atha\AT3G57880
5 of 9
No
Yes
Atha\AT4G00700
5 of 9
No
Yes
Atha\AT4G11610
5 of 9
No
Yes
Atha\AT4G20080
5 of 9
No
Yes
Atha\AT5G12970
5 of 9
No
Yes
Atha\AT5G48060
5 of 9
No
Yes
Atha\FTIP1
5 of 9
No
Yes
Atha\AT3G03680
4 of 9
No
Yes
Atha\AT5G17980
4 of 9
No
Yes
Atha\QKY
4 of 9
No
Yes
Atha\AT3G61720
3 of 9
No
Yes
Atha\AT5G03435
3 of 9
No
Yes
Atha\AT5G44760
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\INN1
1 of 15
Yes
Yes
Scer\TCB1
1 of 15
Yes
No
Scer\TCB2
1 of 15
Yes
No
Scer\TCB3
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\fic1
1 of 12
Yes
Yes
Spom\SPCC962.01
1 of 12
Yes
No
Spom\syn1
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902GZ )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005786
Drosophila simulans
Dsim\GD11410
Drosophila sechellia
Dsec\GM21916
Drosophila erecta
Dere\GG21924
Drosophila yakuba
Dyak\GE11998
Drosophila ananassae
Dana\GF11116
Drosophila pseudoobscura pseudoobscura
Dpse\GA17320
Drosophila persimilis
Dper\GL10771
Drosophila willistoni
Dwil\GK20924
Drosophila virilis
Dvir\GJ20296
Drosophila mojavensis
Dmoj\GI19221
Drosophila grimshawi
Dgri\GH22086
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150186 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17007541
Glossina morsitans
Tsetse fly
Gmor\GMOY007272
Mayetiola destructor
Hessian fly
Mdes\Mdes001660
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006881
Anopheles darlingi
American malaria mosquito
Adar\ADAC000645
Anopheles gambiae
Malaria mosquito
Agam\AGAP007646
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ017690
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00GO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA013776
Danaus plexippus
Monarch butterfly
Dple\DPOGS211535-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS208658-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003126
Heliconius melpomene
Postman butterfly
Hmel\HMEL011750
Apis florea
Little honeybee
Aflo\2158506852
Apis mellifera
Western honey bee
Amel\GB48160
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP23184
Bombus terrestris
Buff-tailed bumblebee
Bter\15209454
Linepithema humile
Argentine ant
Lhum\LH22172
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00124f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004112
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_05227
Tribolium castaneum
Red flour beetle
Tcas\TC014537
Pediculus humanus
Human body louse
Phum\PHUM388330
Rhodnius prolixus
Kissing bug
Rpro\RPRC010303
Cimex lectularius
Bed bug
Clec\CLEC005966
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001811
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_12278
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00FK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012709
Ixodes scapularis
Black-legged tick
Isca\ISCW005839
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81461.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g06520
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_213473
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02XX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mctp1L
Ciona intestinalis
Vase tunicate
Cint\LOC101242966
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000020140
Gallus gallus
Domestic chicken
Ggal\MCTP2
Gallus gallus
Domestic chicken
Ggal\MCTP1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Esyt2
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCTP1; multiple C2 and transmembrane domain containing 1
    13 of 15
    616296
        MCTP2; multiple C2 and transmembrane domain containing 2
        11 of 15
        616297
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Interactions Browser
            Summary of Genetic Interactions
            esyN Network Diagram
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map

            2-86

            Cytogenetic map
            55F1-55F2
            Sequence location
            2R:18,761,758..18,774,824 [+]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            55F1-55F2
            Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)0370903709
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            2-86

            (FlyBase, 2016)

            2-91.3

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (7)
            Genomic Clones (17)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (16)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: Mctp CG33148 CG33146

            (Shin et al., 2005)
            Source for database merge of

            Source for merge of: CG33148 CG33146

            (Shin et al., 2005)
            Additional comments

            Annotations CG33146 and CG33148 merged as CG15078 (which corresponds to Mctp) in release 5.1 of the genome annotation.

            (FlyBase Genome Annotators, 2006)

            Annotation CG15078 split into CG33146, CG33148 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)
            Other Comments

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 46 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DPiM - Drosophila Protein interaction map
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Synonyms and Secondary IDs (7)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (36)