FlyBase Gene Report: Dmel\Mctp

Calcium sensor which is essential for the stabilization of normal baseline neurotransmitter release and for the induction and long-term maintenance of presynaptic homeostatic plasticity (PubMed:28485711).

(UniProt, A1ZBD6)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Mctp or the JBrowse view of Dmel\Mctp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.56

Stop-codon suppression (UAG) postulated; FBrf0216884

gene_with_stop_codon_read_through ; SO:0000697

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mctp-RA

FBtr0110970

NM_137528

4066

893

Mctp-RB

FBtr0110971

NM_001043095

2824

596

Mctp-RD

FBtr0330004

NM_001274149

4123

982

Mctp-RE

FBtr0330005

NM_001043094

4015

954

Mctp-RF

FBtr0345950

NM_001299661

4223

893

Mctp-RG

FBtr0346955

NM_001316428

4123

912

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Mctp-PA

FBpp0110270

101.1

893

7.24

NP_611372

AAO41353

Mctp-PB

FBpp0110271

69.1

596

7.52

NP_001036560

AAF57640

Mctp-PD

FBpp0303039

110.6

982

8.26

NP_001261078

AGB93610

Mctp-PE

FBpp0303040

108.1

954

8.22

NP_001036559

ABI31105

Mctp-PF

FBpp0311864

101.1

893

7.24

NP_001286590

AHN56385

Mctp-PG

FBpp0312419

103.5

912

7.67

NP_001303357

ALI30186

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

893 aa isoforms: Mctp-PA, Mctp-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mctp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

calcium ion binding

inferred from direct assay

(assigned by UniProt )

(Genç et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

calcium ion binding

inferred from sequence or structural similarity with HGNC:26183

(Shin et al., 2005)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

regulation of neuronal synaptic plasticity

inferred from mutant phenotype

(assigned by UniProt )

(Genç et al., 2017)

regulation of neurotransmitter secretion

inferred from mutant phenotype

(assigned by UniProt )

(Genç et al., 2017)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

endoplasmic reticulum membrane

inferred from direct assay

(assigned by UniProt )

(Genç et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

integral component of membrane

inferred from sequence or structural similarity with HGNC:26183

(Shin et al., 2005)

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

endoplasmic reticulum membrane

inferred from direct assay

(assigned by UniProt )

(Genç et al., 2017)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mctp in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mctp

View exonic expression by tissue for Dmel\Mctp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034389

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034389

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33146-RA;CG33148-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33146

Flygut - An atlas of the Drosophila adult midgut

FBgn0034389

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs and Phenotypes

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Allele of Mctp

Class

Mutagen

Associated Insertion

Stocks

Known lesion

Mctp^e00415

piggyBac activity

Yes

Yes

Yes

phiC31 integrase

Mi{Trojan-GAL4.1}Mctp^{MI14773-TG4.1}

Yes

Mctp^BG01140b

P-element activity

P{GT1}Mctp^BG01140b

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mctp

Allele of Mctp

Mutagen

Associated Transgenic Construct

Stocks

Transgenic constructs containing regulatory region of Mctp

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)Exel7158

(Ryder, 2004.4.26)

Summary of Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Mctp^BG01140b

viable

Mctp^e00415

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mctp^GD2076

viable, with Scer\GAL4^Mef2.PR

Mctp^GD2076

viable, with Scer\GAL4^pnr-MD237

Mctp^GD2076

viable, with Scer\GAL4^tin.CΔ4

Mctp^GD2076

Sterility

Allele

fertile

Mctp^e00415

Other Phenotypes

Allele

heat stress response defective | third instar larval stage, with Scer\GAL4^ppk.1.9

Mctp^KK104930

neuroanatomy defective | third instar larval stage, with Scer\GAL4^ppk.1.9

Mctp^KK104930

Phenotype manifest in

Allele

dendrite | third instar larval stage, with Scer\GAL4^ppk.1.9

Mctp^KK104930

dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.1.9

Mctp^KK104930

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MCTP1

13 of 15

Yes

Hsap\MCTP2

11 of 15

Yes

Hsap\ESYT1

1 of 15

Hsap\ESYT2

1 of 15

Hsap\ESYT3

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Mctp1

12 of 15

Yes

Mmus\Mctp2

11 of 15

Yes

Mmus\Esyt1

1 of 15

Mmus\Esyt2

1 of 15

Mmus\Esyt3

1 of 15

Rattus norvegicus (Norway rat) (2)

Rnor\Mctp2

10 of 13

Yes

Rnor\Mctp1

8 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\mctp1

6 of 12

Yes

Xtro\mctp2

6 of 12

Yes

Xtro\esyt3

1 of 12

Danio rerio (Zebrafish) (7)

Drer\mctp1b

13 of 15

Yes

Drer\mctp1a

8 of 15

Yes

Drer\mctp2a

7 of 15

Yes

Drer\mctp2b

5 of 15

Yes

Drer\esyt2a

1 of 15

Drer\esyt2b

1 of 15

Drer\esyt3

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\mctp-1

11 of 15

Yes

Cele\clec-176

1 of 15

Yes

Cele\esyt-2

1 of 15

Cele\snt-2

1 of 15

Arabidopsis thaliana (thale-cress) (18)

Atha\AT3G61300

8 of 9

Yes

Atha\AT5G12970

7 of 9

Yes

Atha\AT1G04150

6 of 9

Yes

Atha\AT1G22610

6 of 9

Yes

Atha\AT1G51570

6 of 9

Yes

Atha\AT3G57880

6 of 9

Yes

Atha\AT4G00700

6 of 9

Yes

Atha\AT4G11610

6 of 9

Yes

Atha\AT4G20080

6 of 9

Yes

Atha\AT5G48060

6 of 9

Yes

Atha\FTIP1

6 of 9

Yes

Atha\AT3G03680

5 of 9

Yes

Atha\AT5G17980

5 of 9

Yes

Atha\QKY

5 of 9

Yes

Atha\AT3G61720

4 of 9

Yes

Atha\AT5G03435

4 of 9

Yes

Atha\AT5G44760

4 of 9

Yes

Atha\AT1G73580

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\INN1

1 of 15

Yes

Scer\TCB1

1 of 15

Yes

Scer\TCB2

1 of 15

Yes

Scer\TCB3

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\SPCC962.01

2 of 12

Yes

Spom\fic1

1 of 12

Yes

Spom\syn1

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902GZ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Mctp

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17320

Drosophila persimilis

Dper\GL10771

Drosophila willistoni

Dwil\GK20924

Drosophila virilis

Dvir\GJ20296

Drosophila mojavensis

Dmoj\GI19221

Drosophila grimshawi

Dgri\GH22086

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150186 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17007541

Glossina morsitans

Tsetse fly

Gmor\GMOY007272

Mayetiola destructor

Hessian fly

Mdes\Mdes001660

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006881

Anopheles darlingi

American malaria mosquito

Adar\ADAC000645

Anopheles gambiae

Malaria mosquito

Agam\AGAP007646

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017690

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00GO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA013776

Danaus plexippus

Monarch butterfly

Dple\DPOGS211535-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS208658-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL003126

Heliconius melpomene

Postman butterfly

Hmel\HMEL011750

Apis florea

Little honeybee

Aflo\2158506852

Apis mellifera

Western honey bee

Amel\GB48160

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP23184

Bombus terrestris

Buff-tailed bumblebee

Bter\15209454

Linepithema humile

Argentine ant

Lhum\LH22172

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00124f

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004112

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05227

Tribolium castaneum

Red flour beetle

Tcas\TC014537

Pediculus humanus

Human body louse

Phum\PHUM388330

Rhodnius prolixus

Kissing bug

Rpro\RPRC010303

Cimex lectularius

Bed bug

Clec\CLEC005966

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001811

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12278

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00FK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012709

Ixodes scapularis

Black-legged tick

Isca\ISCW005839

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81461.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g06520

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_213473

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02XX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Mctp1L

Ciona intestinalis

Vase tunicate

Cint\LOC101242966

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000020140

Gallus gallus

Domestic chicken

Ggal\MCTP2

Gallus gallus

Domestic chicken

Ggal\MCTP1

Human Disease Model Data

FlyBase Human Disease Model Reports

Alleles Reported to Model Human Disease (Disease Ontology)

Download

Models ( 0 )

Allele

Disease

Evidence

References

Interactions ( 0 )

Allele

Disease

Interaction

References

Comments ( 0 )

Human Orthologs (via DIOPT v7.1)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

Complementation?

Transgene?

MCTP1; multiple C2 and transmembrane domain containing 1

13 of 15

616296

MCTP2; multiple C2 and transmembrane domain containing 2

11 of 15

616297

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62835

DroID - A comprehensive database of gene and protein interactions.

FBgn0034389

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37165

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-86

Cytogenetic map

55F1-55F2

Sequence location

2R:18,761,758..18,774,824 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55F1-55F2

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Bloomington

17855

w¹¹¹⁸; PBac{RB}Mctp^e00415

36257

y¹ w^*; Mi{MIC}Mctp^MI02901

59726

y¹ w^*; Mi{MIC}Mctp^MI14773

76765

y¹ w^*; Mi{Trojan-GAL4.1}Mctp^{MI14773-TG4.1}/SM6a

e00415

v100312

v103778

v10061

w¹¹¹⁸; P{GD2076}v10061/TM3

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (16)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

DPiM_RE18318

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034389

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GM06083

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034389

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37165

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Mctp CG33148 CG33146

(Shin et al., 2005)

Source for database merge of

Source for merge of: CG33148 CG33146

(Shin et al., 2005)

Additional comments

Annotations CG33146 and CG33148 merged as CG15078 (which corresponds to Mctp) in release 5.1 of the genome annotation.

(FlyBase Genome Annotators, 2006)

Annotation CG15078 split into CG33146, CG33148 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 45 )

Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37165

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1ZBD6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7JZN2

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034389

InterPro - A database of protein families, domains and functional sites

Linkouts

BioGRID - A database of protein and genetic interactions.

62835

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones

FBgn0034389

DPiM - Drosophila Protein interaction map

FBgn0034389

DroID - A comprehensive database of gene and protein interactions.

FBgn0034389

DRSC - Results frm RNAi screens

FBgn0034389

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034389

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33146-RA;CG33148-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33146

Flygut - An atlas of the Drosophila adult midgut

FBgn0034389

FlyMine - An integrated database for Drosophila genomics

FBgn0034389

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37165

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37165

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG15078

modMine - A data warehouse for the modENCODE project

FBgn0034389

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG15078

(Honjo et al., 2016, Keleman et al., 2009.8.5, FlyBase Genome Annotators, 2006)

CG33146

(Shin et al., 2005, Giot et al., 2003)

CG33148

(Shin et al., 2005)

DmMCTP

(Shin et al., 2005)

Mctp

(Chen et al., 2016, Gene Disruption Project members, 2016-, Honjo et al., 2016, Japanese National Institute of Genetics, 2012.5.21, Tunstall et al., 2012, Jungreis et al., 2011, Gan et al., 2010, Kong et al., 2010)

mctp

(Genç et al., 2017)

Name Synonyms

Multiple C2 domain and transmembrane region protein

(Shin et al., 2005)

Secondary FlyBase IDs

FBan0033146
FBgn0053146
FBgn0053148

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (33)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections