FlyBase Gene Report: Dmel\CG9416

General Information

Symbol

Dmel\CG9416

Species

D. melanogaster

Name

Annotation Symbol

CG9416

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034438

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

56D2-56D2

Sequence location

2R:19,367,248..19,373,702 [-]

Recombination map

2-89

RefSeq locus

NT_033778 REGION:19367248..19373702

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables metalloexopeptidase activity

inferred from electronic annotation with InterPro:IPR045175

(InterPro Project Members, 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from biological aspect of ancestor with PANTHER:PTN000254534

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR045175

(InterPro Project Members, 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

M28 METALLOEXOPEPTIDASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

M28 METALLOEXOPEPTIDASES -: M28 metalloexopeptidases belong to MEROPS family M28, containing aminopeptidases and carboxypeptidases that have co-catalytic zinc ions.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG9416 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8MT48)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

One of six similar genes in a tandem array: CG11961, CG10051, CG9416, CG10062, CG10073, CG10081.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG9416-RA

FBtr0086555

NM_137571

3076

861

CG9416-RB

FBtr0334008

NM_001274159

2936

861

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG9416-PA

FBpp0085739

98.1

861

6.33

FBpp0306127

98.1

861

6.33

Q8MT48

NP_001261088

AGB93620

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

861 aa isoforms: CG9416-PA, CG9416-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q8MT48

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG9416 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.87

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

trunk visceral muscle primordium

(Sandmann et al., 2006)

embryonic stage 11 -- 12

circular trunk visceral muscle primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

esophageal visceral muscle primordium

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

(Fisher et al., 2012)

embryonic stage 12

mesoderm

(Cunha et al., 2010)

embryonic stage 13

somatic muscle cell

(Sandmann et al., 2006)

embryonic stage 13 -- 16

circular intestinal visceral muscle fiber

(Fisher et al., 2012)

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

embryonic/larval esophageal visceral muscle

(Fisher et al., 2012)

longitudinal intestinal visceral muscle fiber

(Fisher et al., 2012)

embryonic/larval plasmatocyte

(Fisher et al., 2012)

Additional Descriptive Data

Expression initiates in the longitudinal visceral muscle precursors at embryonic stage 10 and in the somatic muscle at stage 13.

(Sandmann et al., 2006)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{CG9416.H-Stinger}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

trunk visceral muscle primordium

(Sandmann et al., 2006)

embryonic stage 13

somatic muscle cell

(Sandmann et al., 2006)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG9416 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG9416

View exonic expression by tissue for Dmel\CG9416

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034438

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37223/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034438

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034438

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034438

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9416

Flygut - An atlas of the Drosophila adult midgut

FBgn0034438

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG9416

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG9416

Transgenic constructs containing regulatory region of CG9416

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG9416^GD2088

viable, with Scer\GAL4^Mef2.PR

CG9416^GD2088

viable, with Scer\GAL4^pnr-MD237

CG9416^GD2088

viable, with Scer\GAL4^tin.CΔ4

CG9416^GD2088

Other Phenotypes

Allele

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

CG9416^GD2088

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

Hsap\ERMP1

14 of 14

Yes

Hsap\CPQ

1 of 14

Yes

Hsap\FOLH1

1 of 14

Yes

Hsap\NAALAD2

1 of 14

Yes

Hsap\NAALADL1

1 of 14

Yes

Hsap\NAALADL2

1 of 14

Yes

Hsap\TFRC

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Ermp1

14 of 14

Yes

Rnor\Cpq

1 of 14

Yes

Rnor\Folh1

1 of 14

Yes

Rnor\Naalad2

1 of 14

Yes

Rnor\Naaladl1

1 of 14

Yes

Rnor\Tfr2

1 of 14

Yes

Rnor\Tfrc

1 of 14

Yes

Mus musculus (laboratory mouse) (7)

Mmus\Ermp1

13 of 14

Yes

Mmus\Cpq

1 of 14

Yes

Mmus\Folh1

1 of 14

Yes

Mmus\Naalad2

1 of 14

Yes

Mmus\Naaladl1

1 of 14

Yes

Mmus\Tfr2

1 of 14

Yes

Mmus\Tfrc

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\ermp1

7 of 13

Yes

Xtro\niban2

1 of 13

Yes

Danio rerio (Zebrafish) (4)

Drer\ermp1

1 of 14

Yes

Drer\LOC100330918

1 of 14

Yes

Drer\naalad2

1 of 14

Yes

Drer\naaladl1

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\B0495.7

14 of 14

Yes

Cele\C44B7.11

9 of 14

Yes

Cele\gcp-2.2

1 of 14

Yes

Cele\gcp-2.3

1 of 14

Yes

Cele\Y39A3B.1

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP007676

11 of 12

Yes

Agam\AgaP_AGAP007677

11 of 12

Yes

Agam\AgaP_AGAP003422

7 of 12

Yes

Agam\AgaP_AGAP003423

7 of 12

Yes

Agam\AgaP_AGAP003432

7 of 12

Yes

Agam\AgaP_AGAP003078

6 of 12

Yes

Agam\AgaP_AGAP001264

1 of 12

Yes

Agam\AgaP_AGAP013078

1 of 12

Yes

Agam\AgaP_AGAP013443

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (5)

Atha\AT1G67420

12 of 13

Yes

Atha\AT5G20660

9 of 13

Atha\AMP1

1 of 13

Yes

Atha\AT4G07670

1 of 13

Yes

Atha\AT5G19740

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\PFF1

7 of 13

Yes

Scer\APE3

1 of 13

Scer\TRE1

1 of 13

Yes

Scer\TRE2

1 of 13

Yes

Scer\VPS70

1 of 13

Yes

Scer\YDR415C

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\SPCC1259.02c

11 of 12

Yes

Spom\SPCC1919.12c

4 of 12

Yes

Spom\SPBC354.09c

1 of 12

Yes

Escherichia coli (enterobacterium) (2)

Ecol\iap

1 of 11

Yes

Ecol\yfbL

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG9416. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

12 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ERMP1; endoplasmic reticulum metallopeptidase 1

14 of 14

611156

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62887

DroID - A comprehensive database of gene and protein interactions.

FBgn0034438

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-89

Cytogenetic map

56D2-56D2

Sequence location

2R:19,367,248..19,373,702 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56D2-56D2

Limits computationally determined from genome sequence between P{PZ}ena⁰²⁰²⁹&P{lacW}cora^k08713 and P{lacW}hts^k06121

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-89

(FlyBase, 2016)

2-94.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

602391

y¹ w¹; P{TKO-UAS.GS06198}attP40/CyO

34247

y¹ w^*; Mi{MIC}CG9416^MI01781

59449

y¹ w^*; Mi{MIC}CG9416^MI13342

95065

y¹ w^*; TI{SA-KozakGAL4}CG9416^CR70382-kG4/SM6a

57563

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04880}attP40

604729

y¹ sc^* v¹ sev²¹; P{TOE.GS02968}attP40/CyO

NIG-Fly

2RG-1121

y² VhaAC39-1^cho-1 v¹; P{U6-CG9416.NIG.sgRNA}attP40/CyO

VDRC

v106330

P{KK109223}VIE-260B

v10064

w¹¹¹⁸; P{GD2088}v10064

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (171)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE28322

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034438

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034438

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

CG11961 appears to be a chimeric gene, with the 5' part of the gene derived from CG9416 and the 3' part of the gene derived from CG30049.

(Rogers and Hartl, 2012)

"CG11961" is a putative chimeric gene derived from the "CG9416" and "CG30049" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene).

(Rogers et al., 2009)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG9416

(National Institute of Genetics Fly Stocks, 2022-, Wang et al., 2021, Rice et al., 2020, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Ciglar et al., 2014, Soshnev et al., 2013, Christis and Munro, 2012, Rogers and Hartl, 2012, Cammarato et al., 2011, Cunha et al., 2010, Ellis and Carney, 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Beltran et al., 2007, Firth and Baker, 2007, Zeitlinger et al., 2007, Sandmann et al., 2006)

CG9416-PA

(Rogers et al., 2009)

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37223

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8MT48

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q8MT48

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8MT48

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034438

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37223/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034438

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034438

FlyMine - An integrated database for Drosophila genomics

FBgn0034438

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9416

MARRVEL_MODEL - MARRVEL (model organism gene)

37223

Linkouts

BioGRID - A database of protein and genetic interactions.

62887

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034438

DroID - A comprehensive database of gene and protein interactions.

FBgn0034438

DRSC - Results frm RNAi screens

FBgn0034438

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034438

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034438

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9416

Flygut - An atlas of the Drosophila adult midgut

FBgn0034438

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

References (47)

The following external sites may use different assemblies or annotations than FlyBase.

-0.87

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