FlyBase Gene Report: Dmel\Toll-7

General Information

Symbol

Dmel\Toll-7

Species

D. melanogaster

Name

Toll-7

Annotation Symbol

CG8595

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034476

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Toll-7 (Toll-7) encodes a plasma membrane Toll receptor that binds to viral glycoproteins, leading to the activation of antiviral defenses including autophagy. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

Tl-7

Key Links

Genomic Location

Cytogenetic map

56E4-56E4

Sequence location

2R:19,826,578..19,832,973 [+]

Recombination map

2-90

RefSeq locus

NT_033778 REGION:19826578..19832973

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables neurotrophin receptor activity

inferred from mutant phenotype

(McIlroy et al., 2013)

enables transmembrane signaling receptor activity

inferred from direct assay

(Chowdhury et al., 2019)

enables virion binding

inferred from direct assay

(Chowdhury et al., 2019, Nakamoto et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables pattern recognition receptor activity

inferred by curator from GO:0009897,GO:0010508,GO:0046790

(Nakamoto et al., 2012)

Biological Process (9 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in axon guidance

inferred from mutant phenotype

(Ward et al., 2015)

involved_in defense response to virus

inferred from mutant phenotype

(Nakamoto et al., 2012)

involved_in detection of virus

inferred from direct assay

(Chowdhury et al., 2019)

involved_in innate immune response

inferred from mutant phenotype

(Chowdhury et al., 2019)

involved_in positive regulation of antimicrobial peptide production

inferred from mutant phenotype

(Chowdhury et al., 2019)

involved_in positive regulation of autophagy

inferred from mutant phenotype

(Nakamoto et al., 2012)

involved_in regulation of defense response to virus

inferred from mutant phenotype

(Moy et al., 2014)

involved_in Toll signaling pathway

inferred from mutant phenotype

(Chowdhury et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in cell surface pattern recognition receptor signaling pathway

inferred by curator from GO:0009897,GO:0010508,GO:0046790

(Nakamoto et al., 2012)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in external side of plasma membrane

inferred from direct assay

(Nakamoto et al., 2012)

located_in integral component of plasma membrane

inferred from direct assay

(Chowdhury et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN001224703

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN001224703

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

TOLL RECEPTORS

Pathway (FlyBase)

Toll-NF-κB Signaling Pathway Core Components

Protein Family (UniProt)

Belongs to the Toll-like receptor family. (Q7KIN0)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Toll-7 (Toll-7) encodes a plasma membrane Toll receptor that binds to viral glycoproteins, leading to the activation of antiviral defenses including autophagy. [Date last reviewed: 2018-10-18]

Gene Group (FlyBase)

TOLL RECEPTORS -: The Toll/Toll-like receptor (TLR) family members are type-I single-pass transmembrane receptors with an intracellular Toll IL-1R homology (TIR) domain and extracellular Leucine-rich repeats. The archetypal family member, Tl, was first characterized for its role in embryonic dorsal/ventral patterning. In larval and adult stages, Tl is involved with innate immune response. Tl is activated by binding an endogenous spatzle ligand and activation of NF-κB is the target of canonical Toll/Toll-like receptor signaling. Non-canonical signaling pathways are less well characterized. (Adapted from FBrf0130108, FBrf0212564 and FBrf0239503).

Pathway (FlyBase)

Toll-NF-κB Signaling Pathway Core Components -: In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. (Adapted from FBrf0091014 and FBrf0223077).

Protein Function (UniProtKB)

Toll-related receptor which binds to the neurotrophins NT1 and spz5 (PubMed:10973475, PubMed:23892553). Essential for antiviral autophagy, it detects and binds to the vesicular stomatitis virus (vsv) following infection (PubMed:22464169). This role is likely to be independent of the canonical Toll, immune deficiency, and JAK-STAT signaling pathways (PubMed:22464169). Functions in olfactory circuit assembly by promoting synaptic partner matching between olfactory receptor neurons (ORN) axons and projection neurons (PN) dendrites partners in the antennal lobe (PubMed:25741726). Function in the Va1d ORNs is necessary and sufficient for correct targeting to their partner PN dendrites (PubMed:25741726). Also involved in the targeting of other classes of ORN axons (PubMed:25741726). Functions with Toll-6 to regulate motor axon targeting and neuronal survival in the central nervous system (CNS) (PubMed:23892553). May be an upstream component of the NF-kappa-B (rel) regulatory cascade (PubMed:23892553).

(UniProt, Q7KIN0)

Summary (Interactive Fly)

Toll-like receptors - Neurotrophin receptors - regulation of locomotion, motor axon targeting and neuronal survival - innate immunity

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Toll-7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Toll-7-RA

FBtr0086336

NM_079073

6396

1446

Additional Transcript Data and Comments

Reported size (kB)

6.4, 5.2 (northern blot)

(Tauszig et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Toll-7-PA

FBpp0085638

160.9

1446

6.59

NP_523797

AAF57514

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

1446 (aa)

(Tauszig et al., 2000)

Comments

External Data

Domain

The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

(UniProt, Q7KIN0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Toll-7 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Toll-7_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism | restricted

(Kambris et al., 2002)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11

organism | segment polarity expression pattern

(Kambris et al., 2002)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior spiracle primordium

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

embryonic stage 14

embryonic/larval neuron | subset

(Kambris et al., 2002)

ventral thoracic disc primordium

(Kambris et al., 2002)

embryonic large intestine

(Kambris et al., 2002)

embryonic/larval anterior spiracle

(Kambris et al., 2002)

embryonic/larval posterior spiracle

(Kambris et al., 2002)

embryonic stage 16

embryonic/larval central nervous system

(Kambris et al., 2002)

wandering third instar larval stage

wing hinge primordium

(Yagi et al., 2010)

mesothoracic tergum primordium

(Yagi et al., 2010)

presumptive tarsal segment 2 -- presumptive tarsal segment 5

(Yagi et al., 2010)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Tauszig et al., 2000)

adult stage | male

(Tauszig et al., 2000)

Additional Descriptive Data

Tollo transcript is expressed in the proximal region of the wing disc, and distal segments of the leg discs; expression is absent from the wing pouch.

(Yagi et al., 2010)

Toll-7 is expressed dynamically throughout embryogenesis. At the beginning of germ band extension, a strong spot is seen in the head region and diffuse staining in the trunk. At full germ band extension, a pattern of 14 strips is seen and they are positioned posterior to the wg stripes. At germ band retraction, Toll-7 is expressed in some neurons of the CNS, in the precursors to the leg imaginal discs, in the ventral epithelium of the large intestine, and in the anterior and posterior spiracles. At stage 16 Toll-7 is reduced and is mostly restricted to the CNS.

(Kambris et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8 -- adult stage

antennal lobe glomerulus DA1

(Ward et al., 2015)

antennal lobe glomerulus VA1d

(Ward et al., 2015)

antennal lobe glomerulus VA1v

(Ward et al., 2015)

adult antennal lobe commissure | restricted

(Ward et al., 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Toll-7-protein is enriched in a cluster of glomeruli in the anterior and lateral regions of the antennal lobe, which include the DA1, VA1d and VA1v glomeruli.

(Ward et al., 2015)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in external side of plasma membrane

inferred from direct assay

(Nakamoto et al., 2012)

located_in integral component of plasma membrane

inferred from direct assay

(Chowdhury et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Toll-7 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Toll-7

View exonic expression by tissue for Dmel\Toll-7

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034476

DRscDB

37272/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034476

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8595-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8595

Flygut - An atlas of the Drosophila adult midgut

FBgn0034476

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Toll-7

Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Toll-7

Transgenic constructs containing regulatory region of Toll-7

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Toll-7^AW1

(Ward et al., 2015)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Toll-7^g1-1

lethal, with Scer\GAL4^e22c

Toll-7^UAS.cYa

lethal, with Scer\GAL4^ptc-559.1

Toll-7^UAS.cYa

partially lethal - majority die (with Df(2R)BSC22)

Toll-7^g1-1

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Toll-7^GD14417

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Toll-7^GD14417

Other Phenotypes

Allele

abnormal immune response, with Scer\GAL4^Act.PU

Toll-7^GD14417

abnormal immune response (with Df(2R)BSC22)

Toll-7^g1-5

abnormal neuroanatomy, with Scer\GAL4^elav-C155

Toll-7^{C993Y.UAS.Tag:FLAG}

abnormal neuroanatomy | adult stage, with Scer\GAL4^AM29

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

decreased cell death, with Scer\GAL4^elav-C155

Toll-7^{C993Y.UAS.Tag:FLAG}

increased cell number | third instar larval stage, with Scer\GAL4^elav.PU

Toll-7^{C993Y.UAS.Tag:FLAG}

Phenotype manifest in

Allele

adult abdomen, with Scer\GAL4^32B

Toll-7^UAS.cYa

adult abdomen, with Scer\GAL4^da.G32

Toll-7^UAS.cYa

adult antennal lobe projection neuron VA1d adPN, with Scer\GAL4^elav-C155

adult olfactory receptor neuron Or67a, with Scer\GAL4^AM29

adult olfactory receptor neuron Or88a, with Scer\GAL4^elav-C155

embryonic/larval central nervous system, with Scer\GAL4^elav-C155

Toll-7^{C993Y.UAS.Tag:FLAG}

eye, with Scer\GAL4^da.G32

Toll-7^UAS.cYa

eye, with Scer\GAL4^GMR.PF

Toll-7^UAS.cYa

eye, with Scer\GAL4^lz-gal4

Toll-7^UAS.cYa

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^elav.PU

Toll-7^{C993Y.UAS.Tag:FLAG}

leg, with Scer\GAL4^dpp.PS

Toll-7^UAS.cYa

macrochaeta | ectopic, with Scer\GAL4^71B

Toll-7^UAS.cYa

macrochaeta | ectopic, with Scer\GAL4^da.G32

Toll-7^UAS.cYa

neuron | increased number | third instar larval stage, with Scer\GAL4^elav.PU

Toll-7^{C993Y.UAS.Tag:FLAG}

ventral thoracic disc, with Scer\GAL4^dpp.PS

Toll-7^UAS.cYa

wing, with Scer\GAL4^dpp.PS

Toll-7^UAS.cYa

wing disc, with Scer\GAL4^dpp.PS

Toll-7^UAS.cYa

wing vein, with Scer\GAL4^da.G32

Toll-7^UAS.cYa

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (43)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TLR3

4 of 15

Yes

Hsap\TLR7

3 of 15

Yes

Hsap\TLR9

3 of 15

Yes

Hsap\CD180

2 of 15

Hsap\CHADL

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Yes

Hsap\ELFN2

1 of 15

Yes

Hsap\GP1BA

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\LGI3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\LRRC3B

1 of 15

Hsap\LRRC3C

1 of 15

Yes

Hsap\LRRC24

1 of 15

Hsap\LRRC32

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\RTN4RL2

1 of 15

Hsap\TRIL

1 of 15

Hsap\VASN

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (47)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Tlr13

4 of 15

Yes

Mmus\Tlr3

3 of 15

Mmus\Tlr7

3 of 15

Yes

Mmus\Tlr9

3 of 15

Yes

Mmus\Cd180

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Elfn2

1 of 15

Yes

Mmus\Gp1ba

1 of 15

Yes

Mmus\Hmcn2

1 of 15

Mmus\Lgi1

1 of 15

Mmus\Lgi2

1 of 15

Yes

Mmus\Lgi3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Lrrc3b

1 of 15

Mmus\Lrrc3c

1 of 15

Yes

Mmus\Lrrc15

1 of 15

Mmus\Lrrc24

1 of 15

Mmus\Lrrc32

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Nyx

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Vasn

1 of 15

Yes

Rattus norvegicus (Norway rat) (47)

Rnor\Tlr3

3 of 13

Yes

Rnor\Tlr7

3 of 13

Yes

Rnor\Cd180

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Elfn2

1 of 13

Yes

Rnor\Gp1ba

1 of 13

Yes

Rnor\Igfals

1 of 13

Rnor\Lgi1

1 of 13

Rnor\Lgi3

1 of 13

Yes

Rnor\Lgi4

1 of 13

Rnor\Lingo1

1 of 13

Rnor\Lingo3

1 of 13

Yes

Rnor\Lingo4

1 of 13

Yes

Rnor\Lrit2

1 of 13

Rnor\Lrrc3b

1 of 13

Rnor\Lrrc66

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Slitrk1

1 of 13

Rnor\Slitrk2

1 of 13

Yes

Rnor\Slitrk3

1 of 13

Yes

Rnor\Slitrk5

1 of 13

Yes

Rnor\Slitrk6

1 of 13

Yes

Rnor\Tlr1

1 of 13

Rnor\Tlr10

1 of 13

Rnor\Tlr13

1 of 13

Yes

Rnor\Tril

1 of 13

Rnor\Tsku

1 of 13

Yes

Rnor\Tspear

1 of 13

Rnor\Vasn

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (38)

Xtro\LOC100485164

4 of 12

Yes

Xtro\tlr3

4 of 12

Yes

Xtro\tlr7

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Yes

Xtro\chadl

1 of 12

Yes

Xtro\chad

1 of 12

Yes

Xtro\elfn1

1 of 12

Yes

Xtro\elfn2

1 of 12

Yes

Xtro\igfals

1 of 12

Xtro\lingo1

1 of 12

Xtro\lingo2

1 of 12

Yes

Xtro\lingo3

1 of 12

Yes

Xtro\lingo4

1 of 12

Xtro\LOC100485927

1 of 12

Yes

Xtro\LOC100497009

1 of 12

Yes

Xtro\LOC100498493

1 of 12

Xtro\LOC101734746

1 of 12

Yes

Xtro\lrrc3b

1 of 12

Yes

Xtro\lrrc3c

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\nyx

1 of 12

Xtro\tlr1

1 of 12

Xtro\tlr8

1 of 12

Yes

Xtro\tril

1 of 12

Xtro\vasn

1 of 12

Yes

Danio rerio (Zebrafish) (55)

Drer\tlr21

4 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

Yes

Drer\tlr8b

3 of 15

Yes

Drer\tlr20.2

3 of 15

Drer\cd180

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Drer\chadlb

1 of 15

Yes

Drer\elfn1a

1 of 15

Yes

Drer\elfn1b

1 of 15

Yes

Drer\elfn2a

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\lgi3

1 of 15

Yes

Drer\lgr4

1 of 15

Drer\lingo1a

1 of 15

Drer\lingo1b

1 of 15

Yes

Drer\lingo2b

1 of 15

Yes

Drer\lingo3a

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\lrrtm4l1

1 of 15

Yes

Drer\lrrtm4l2

1 of 15

Yes

Drer\rtn4rl2a

1 of 15

Yes

Drer\rtn4rl2b

1 of 15

Yes

Drer\si:ch211-180f4.1

1 of 15

Drer\si:dkey-182i3.11

1 of 15

Drer\tlr8a

1 of 15

Yes

Drer\tlr20f

1 of 15

Drer\tsku

1 of 15

Yes

Drer\vasnb

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\tol-1

12 of 15

Yes

Cele\egg-6

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (28)

Atha\RLP12

2 of 9

Yes

Atha\RLP35

2 of 9

Yes

Atha\RLP40

2 of 9

Yes

Atha\AT2G34930

1 of 9

Yes

Atha\GHR1

1 of 9

Atha\RLP6

1 of 9

Yes

Atha\RLP7

1 of 9

Yes

Atha\RLP9

1 of 9

Yes

Atha\RLP14

1 of 9

Yes

Atha\RLP21

1 of 9

Yes

Atha\RLP23

1 of 9

Yes

Atha\RLP24

1 of 9

Yes

Atha\RLP26

1 of 9

Yes

Atha\RLP27

1 of 9

Yes

Atha\RLP28

1 of 9

Yes

Atha\RLP32

1 of 9

Yes

Atha\RLP34

1 of 9

Yes

Atha\RLP38

1 of 9

Yes

Atha\RLP39

1 of 9

Yes

Atha\RLP41

1 of 9

Yes

Atha\RLP42

1 of 9

Yes

Atha\RLP45

1 of 9

Yes

Atha\RLP46

1 of 9

Yes

Atha\RLP47

1 of 9

Yes

Atha\RLP48

1 of 9

Yes

Atha\RLP50

1 of 9

Yes

Atha\RLP53

1 of 9

Yes

Atha\RLP56

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CYR1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900WW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21191

Drosophila persimilis

Dper\GL10362

Drosophila willistoni

Dwil\GK15893

Drosophila virilis

Dvir\GJ20935

Drosophila mojavensis

Dmoj\GI21088

Drosophila grimshawi

Dgri\GH23021

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500VD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17011314

Musca domestica

House fly

Mdoa\17017409

Glossina morsitans

Tsetse fly

Gmor\GMOY009848

Glossina morsitans

Tsetse fly

Gmor\GMOY005227

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09781

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09778

Mayetiola destructor

Hessian fly

Mdes\Mdes006006

Aedes aegypti

Yellow fever mosquito

Aaeg\TOLL7

Anopheles darlingi

American malaria mosquito

Adar\ADAC010060

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016598

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00IS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011038

Bombyx mori

Silkmoth

Bmor\Bm18w

Danaus plexippus

Monarch butterfly

Dple\DPOGS205295-PA

Heliconius melpomene

Postman butterfly

Hmel\TLR-H

Heliconius melpomene

Postman butterfly

Hmel\TLR-I

Heliconius melpomene

Postman butterfly

Hmel\TLR-G

Apis florea

Little honeybee

Aflo\2169835268

Apis mellifera

Western honey bee

Amel\18w

Bombus terrestris

Buff-tailed bumblebee

Bter\15214678

Linepithema humile

Argentine ant

Lhum\LH13356

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00220a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007768

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007767

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02782

Tribolium castaneum

Red flour beetle

Tcas\TC004474

Pediculus humanus

Human body louse

Phum\PHUM480550

Rhodnius prolixus

Kissing bug

Rpro\RPRC015296

Cimex lectularius

Bed bug

Clec\CLEC003287

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008698

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_10053

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00OH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008376

Ixodes scapularis

Black-legged tick

Isca\ISCW020989

Ixodes scapularis

Black-legged tick

Isca\ISCW022740

Stegodyphus mimosarum

African social velvet spider

Smim\KFM66468.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59381.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72911.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur09g04990

Tetranychus urticae

Two-spotted spider mite

Turt\tetur34g01180

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_128614

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_65779

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_10255

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0137 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-TlrL_12

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tlr132

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tlr066

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tlr041

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

18w

6 of 10

Tollo

6 of 10

Toll-6

5 of 10

4 of 10

4 of 10

3 of 10

3 of 10

2 of 10

2 of 10

rdo

2 of 10

Toll-9

2 of 10

trn

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

chp

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

lbk

1 of 10

ltl

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TLR7; toll like receptor 7

model of X-Linked immunodeficiency 74

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Toll-7^HM05230

ameliorates neurodegenerative disease

modeled by Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TLR3; toll like receptor 3

4 of 15

603029

TLR7; toll like receptor 7

3 of 15

300365

IMMUNODEFICIENCY 74, COVID19-RELATED, X-LINKED; IMD74

X-Linked immunodeficiency 74

TLR9; toll like receptor 9

3 of 15

605474

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Toll-7 - Myd88

anti tag coimmunoprecipitation, anti tag western blot

(Foldi et al., 2017)

Toll-7 - NT1

anti tag coimmunoprecipitation, anti tag western blot, enzyme linked immunosorbent assay

(Chowdhury et al., 2019, McIlroy et al., 2013)

Toll-7 - kek6

anti tag coimmunoprecipitation, anti tag western blot

(Ulian-Benitez et al., 2017)

Toll-7 - spz

anti tag coimmunoprecipitation, anti tag western blot

(Chowdhury et al., 2019)

Toll-7 - spz5

comigration in non denaturing gel electrophoresis, anti tag western blot, anti tag coimmunoprecipitation

(Chowdhury et al., 2019, Foldi et al., 2017, McIlroy et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Toll-7

enhanceable

Toll-6

(Yagi et al., 2010)

Toll-7

enhanceable

Tollo

(Yagi et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Myd88

enhanceable

Toll-6|Toll-7

(Foldi et al., 2017)

NT1

suppressible

Toll-7

(McIlroy et al., 2013)

NT1|spz5

suppressible

Toll-7

(McIlroy et al., 2013)

NT1|Toll-6

suppressible

Toll-7

(McIlroy et al., 2013)

spz

suppressible

Toll-7

(McIlroy et al., 2013)

Toll-6

suppressible

Toll-7

(Ward et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62928

DroID - A comprehensive database of gene and protein interactions.

FBgn0034476

MIST (genetic) - An integrated Molecular Interaction Database

Toll-7

MIST (protein-protein) - An integrated Molecular Interaction Database

Toll-7

Pathways

Signaling Pathways (FlyBase)

Toll-NF-κB Signaling Pathway Core Components -: In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. (Adapted from FBrf0091014 and FBrf0223077).

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-90

Cytogenetic map

56E4-56E4

Sequence location

2R:19,826,578..19,832,973 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56E4-56E4

Limits computationally determined from genome sequence between P{PZ}sm⁰⁵³³⁸&P{PZ}emm¹ and P{lacW}l(2)k08002^k08002

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

56E3-56F9

(Tauszig, 2000.3.23)

Experimentally Determined Recombination Data

Location

2-90

(FlyBase, 2016)

2-95.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

64075

w^*; P{UAS-Toll-7.Δcyto.GFP}3

64077

w^*; P{UAS-Toll-7.TIR-dead}attP40

37050

y¹ w^*; Mi{MIC}Toll-7^MI03386

59468

y¹ w^*; Mi{MIC}Toll-7^MI13963/SM6a

30488

y¹ sc^* v¹ sev²¹; P{TRiP.HM05230}attP2

VDRC

v39176

w¹¹¹⁸; P{GD14417}v39176

v6541

w¹¹¹⁸; P{GD16}v6541/TM3

v24473

w¹¹¹⁸; P{GD16}v24473/TM3

v342178

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01730}attP40/CyO-GFP

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034476

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034476

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37272

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ward A., 2015.10.7, Ward et al., 2015, McIlroy et al., 2013, Nakamoto et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Toll-7 CG8595

(FlyBase, 1996-)

Source for database merge of

Additional comments

The Toll-7 gene may have been derived from the chp gene by retroposition.

(Pan and Zhang, 2009)

The 18w and Toll-7 genes share at least 50% identity.

(Langille and Clark, 2007)

Source for identity of Toll-7 CG8595 was sequence comparison ( date:000721 ).

(FlyBase, 1996-)

Other Comments

Toll-6 acts cell autonomously to instruct the targeting of specific classes of olfactory receptor neuron axons.

(Ward et al., 2015)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37272

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q7KIN0

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034476

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034476

DRscDB

37272/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034476

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Toll-7_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8595

Flygut - An atlas of the Drosophila adult midgut

FBgn0034476

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37272

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004474

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8595

MARRVEL_MODEL

37272

modMine - A data warehouse for the modENCODE project

FBgn0034476

Linkouts

BioGRID - A database of protein and genetic interactions.

62928

DroID - A comprehensive database of gene and protein interactions.

FBgn0034476

DRSC - Results frm RNAi screens

FBgn0034476

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8595-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034476

FlyMine - An integrated database for Drosophila genomics

FBgn0034476

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Toll-7

MIST (protein-protein) - An integrated Molecular Interaction Database

Toll-7

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG8595

(Liu et al., 2015, Pan and Zhang, 2009, Perkins et al., 2009, Wasserman, 2000)

CT24947

(Hynes and Zhao, 2000)

TOLL 7

(Christophides et al., 2002)

Tl-7

(Khush and Lemaitre, 2000, Lemaitre, 2000.12.20)

Toll

(Wei et al., 2018)

Toll 7

(Castillo et al., 2015)

Toll-7

(DeAngelis et al., 2021, Michki et al., 2021, Alvarado et al., 2020, Iijima et al., 2020, Li et al., 2020, Liu et al., 2020, Tafesh-Edwards and Eleftherianos, 2020, Bali et al., 2019, Belmonte et al., 2019, Bhattacharjee et al., 2019, Chowdhury et al., 2019, Germani et al., 2018, La Fortezza et al., 2018, Lopez et al., 2018, Foldi et al., 2017, Franz et al., 2017, Levin and Malik, 2017, Ulian-Benitez et al., 2017, Liu et al., 2015, Neyen and Lemaitre, 2015, Vlisidou and Wood, 2015, dos Santos, 2014, Moy et al., 2014, Mulakkal et al., 2014, Paré et al., 2014, Xu and Cherry, 2014, McIlroy et al., 2013, Merkling and van Rij, 2013, Ozkan et al., 2013, Samaraweera et al., 2013, Díaz-Castillo et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Nakamoto et al., 2012, Yagi et al., 2010, Roeder et al., 2009, Swanson et al., 2009, Sandoval and Eldon, 2008, Wagner et al., 2008, Langille and Clark, 2007, Bartolome and Charlesworth, 2006, Ooi et al., 2002, Tauszig-Delamasure et al., 2002)

Toll7

(Graham et al., 2019)

dTLR7

(Silverman and Maniatis, 2001)

dToll7

(Singh et al., 2003)

toll

(Janssens and Beyaert, 2002)

toll-7

(Ward A., 2015.10.7, Ward et al., 2015)

Name Synonyms

Toll-7

(Anthoney et al., 2018, Kuo et al., 2018, Nie et al., 2018, Swevers et al., 2018, Panayidou et al., 2014, Moy and Cherry, 2013, Tauszig, 2000.3.23)

Toll-like

(Hynes and Zhao, 2000)

Toll7

(Palmer et al., 2018)

toll receptor

(Haesemeyer M et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (104)

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