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Gene Dmel\MED8

General Information
Symbol	Dmel\MED8	Species	D. melanogaster
Name	Mediator complex subunit 8	Annotation symbol	CG13867
Feature type	protein_coding_gene	FlyBase ID	FBgn0034503
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	Arc32, dARC32
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	56F16-56F16	Sequence location	2R:16,213,346..16,214,311 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mediator complex subunit 8 is referred to in FlyBase by the symbol Dmel\MED8 (CG13867, FBgn0034503). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II transcription cofactor activity. Based on sequence similarity, it is predicted to be involved in the biological process: transcription initiation from RNA polymerase II promoter. 4 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Mediator complex, subunit Med8, fungi/metazoa. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1630559_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of MED8. Gene sequence location is 2R:16213346..16214311.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN27827 HFA06426
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 56F16-56F16   Limits computationally determined from genome sequence between P{EP}CG10444EP951 and P{EP}l(2)05510EP2587&P{lacW}rigk07917
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\MED8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) MED8-RA FBtr0086297  NM_153772   966   252
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein MED8-PA   FBpp0085609   27.9   252   8.45     NP_722456   AAM68406
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med8, fungi/metazoa (IPR019364)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAM68406   AI514064   AY071108 AAL48730   AY231711   BI173508   BI633331   BQ103314   BT050506   CO186815   CO186950   CO309441   EC057461   EC064316   EC213258   EC217755   EC229330   EV598522
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1ZBT5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0034503    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0034503 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0034503 expression level Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3037) embryo 02-04hr  (2622) embryo 04-06hr  (3333) embryo 06-08hr  (3220) embryo 08-10hr  (2455) embryo 10-12hr  (2524) embryo 12-14hr  (1625) embryo 14-16hr  (1128) embryo 16-18hr  (1024) embryo 18-20hr  (1006) embryo 20-22hr  (908) embryo 22-24hr  (822) larva L1  (1236) larva L2  (1146) larva L3 12hr old  (996) larva L3 puffstage 1-2  (1149) larva L3 puffstage 3-6  (1330) larva L3 puffstage 7-9  (1733) white prepupae new  (1852) white prepupae 12hr  (1789) white prepupae 24hr  (1954) pupae 2d postWPP  (1290) pupae 3d postWPP  (946) pupae 4d postWPP  (665) adult male 01day  (1008) adult male 05day  (1089) adult male 30day  (936) adult female 01day  (1277) adult female 05day  (2026) adult female 30day  (2032) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3037) embryo 02-04hr  (2622) embryo 04-06hr  (3333) embryo 06-08hr  (3220) embryo 08-10hr  (2455) embryo 10-12hr  (2524) embryo 12-14hr  (1625) embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9  (1733) white prepupae new  (1852) white prepupae 12hr  (1789) white prepupae 24hr  (1954) pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day  (2026) adult female 30day  (2032) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0034503 expression level Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3037) embryo 02-04hr  (2622) embryo 04-06hr  (3333) embryo 06-08hr  (3220) embryo 08-10hr  (2455) embryo 10-12hr  (2524) embryo 12-14hr  (1625) embryo 14-16hr  (1128) embryo 16-18hr  (1024) embryo 18-20hr  (1006) embryo 20-22hr  (908) embryo 22-24hr  (822) larva L1  (1236) larva L2  (1146) larva L3 12hr old  (996) larva L3 puffstage 1-2  (1149) larva L3 puffstage 3-6  (1330) larva L3 puffstage 7-9  (1733) white prepupae new  (1852) white prepupae 12hr  (1789) white prepupae 24hr  (1954) pupae 2d postWPP  (1290) pupae 3d postWPP  (946) pupae 4d postWPP  (665) adult male 01day  (1008) adult male 05day  (1089) adult male 30day  (936) adult female 01day  (1277) adult female 05day  (2026) adult female 30day  (2032) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3037) embryo 02-04hr  (2622) embryo 04-06hr  (3333) embryo 06-08hr  (3220) embryo 08-10hr  2455 embryo 10-12hr  (2524) embryo 12-14hr  1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9  1733 white prepupae new  1852 white prepupae 12hr  1789 white prepupae 24hr  1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day  2026 adult female 30day  2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3037 embryo 02-04hr    2622 embryo 04-06hr    3333 embryo 06-08hr    3220 embryo 08-10hr    2455 embryo 10-12hr    2524 embryo 12-14hr    1625 embryo 14-16hr    1128 embryo 16-18hr    1024 embryo 18-20hr    1006 embryo 20-22hr    908 embryo 22-24hr    822 larva L1    1236 larva L2    1146 larva L3 12hr old    996 larva L3 puffstage 1-2    1149 larva L3 puffstage 3-6    1330 larva L3 puffstage 7-9    1733 white prepupae new    1852 white prepupae 12hr    1789 white prepupae 24hr    1954 pupae 2d postWPP    1290 pupae 3d postWPP    946 pupae 4d postWPP    665 adult male 01day    1008 adult male 05day    1089 adult male 30day    936 adult female 01day    1277 adult female 05day    2026 adult female 30day    2032 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0034503    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0034503 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0034503 expression level Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0034503 expression level Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    212.2 Larval Midgut    81 Larval Hindgut    142.3 Larval Malpighian Tubules    115.4 Larval Fat Body    135.9 Larval Salivary Gland    116.6 Larval Trachea    117.325 Larval Carcass    97.8 Adult Head    103.8 Adult Eye    130.925 Adult Brain    117.8 Adult Thoracic-Abdominal Ganglion    140.6 Adult Crop    157.9 Adult Midgut    110.6 Adult Hindgut    122.7 Adult Malpighian Tubules    53.6 Adult Fat Body    109.3 Adult Salivary Gland    127.8 Adult Heart    98.95 Adult VirginFemale Spermatheca    133.6 Adult InseminatedFemale Spermatheca    130.2 Adult Ovary    259.4 Adult Testis    91.2 Adult Male Accessory Gland    96.4 Adult Carcass    134 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    103.8   NA  Eye    130.925   NA  Brain    117.8   212.2  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    140.6   NA  Crop    157.9   81  Midgut    110.6   142.3  Hindgut    122.7   115.4  Malpighian Tubules    53.6   135.9  Fat Body    109.3   116.6  Salivary Gland    127.8   NA  Heart    98.95   117.325  Trachea    NA   NA  VirginFemale Spermatheca    133.6   NA  InseminatedFemale Spermatheca    130.2   NA  Ovary    259.4   NA  Testis    91.2   NA  Male Accessory Gland    96.4   97.8  Carcass    134 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034503 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13867-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 MED8GD8141 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 MED8GD8141 Phenotype manifest in Allele chaeta | supernumerary, with Scer\GAL4pnr-MD237 MED8GD8141 mesothoracic tergum, with Scer\GAL4pnr-MD237 MED8GD8141
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of MED8 Class Mutagen Stocks Known lesion MED8EY21803 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of MED8 Class Mutagen Stocks Known lesion MED8GD8141 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MED8HMS01275 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED8KK107827 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8141} NA P{KK107827} NA P{TRiP.HMS01275} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH0643SH0643 No miscellaneous insertions P{SUPor-P}KG00080 NA insertion of mobile activating element P{EPgy2}MED8EY21803 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function
CV term Evidence References
RNA polymerase II transcription cofactor activity inferred from mutant phenotype (assigned by UniProtKB) (Marr et al., 2006)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
RNA polymerase II transcription cofactor activity inferred from sequence or structural similarity (Boube et al., 2000)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase II promoter inferred from electronic annotation with InterPro:IPR019364 (FlyBase Curators et al., 2004-) transcription from RNA polymerase II promoter inferred by curator from GO:0001104 (assigned by UniProtKB) (Marr et al., 2006) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity (Boube et al., 2000)
Cellular Component
CV term Evidence References
mediator complex inferred by curator from GO:0001104 (assigned by UniProtKB) (Marr et al., 2006) inferred from electronic annotation with InterPro:IPR019364 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Boube et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions MED8 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62960 DroID - A comprehensive database of gene and protein interactions. • FBgn0034503 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37305
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12669 Dere\GG20865 Dgri\GH20059 Dmoj\GI18776 Dper\GL16821 Dpse\GA12583 Dsec\GM19790 Dsim\GD25282 Dvir\GJ21803 Dwil\GK17848 Dyak\Arc32
Curated drosophilid orthologs	Dmir\Arc32 (Bachtrog and Andolfatto, 2006) Dpse\GA12583   Dyak\Arc32
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL010861-PA Aedes aegypti (mosquito) AGAP005909-PA Anopheles gambiae (mosquito) XP_623322.1 Apis mellifera (honey bee) XP_001949753.1 Acyrthosiphon pisum (pea aphid) 118497 Branchiostoma floridae (lancelet) 126473 Branchiostoma floridae (lancelet) BGIBMGA010622-PA Bombyx mori (silk worm) ENSBTAP00000022477 Bos taurus (bovine) CN19971 Caenorhabditis brenneri (small nematode) CE24544 Caenorhabditis elegans (worm) ENSCAFP00000007832 Canis familiaris (dog) ENSCINP00000011588 Ciona intestinalis (sea squirt) JA16572 Caenorhabditis japonica (nematode) ENSCPOP00000005502 Cavia porcellus (guinea pig) RP14671 Caenorhabditis remanei (nematode) ENSCSAVP00000011126 Ciona savignyi (sedentary tunicate) 226423 Capitella spl (polychaete worm) 301758 Daphnia pulex (daphnia) ENSDARP00000091089 Danio rerio (zebra fish) ENSECAP00000008464 Equus caballus (horse) ENSGACP00000005194 Gymnopilus aculeatus (mushroom) ENSGALP00000016218 Gallus gallus (chicken) 185934 Hypsipyla robusta (cedar shoot borer) ENSP00000290663 Homo sapiens (human) ISCW021461-PA Ixodes scapularis (deer tick) 186104 Lottia gigantea (owl limpet) ENSMODP00000014148 Monodelphis domestica (gray opossum) ENSMMUP00000031169 Macaca mulatta (rhesus monkey) ENSMUSP00000019229 Mus musculus (house mouse) 172778 Nematostella vectensis (starlet anemone) XP_001608087.1 Nasonia vitripennis (wasp) ENSOANP00000013051 Ornithorynchus anatinus (platypus) ENSORLP00000017797 Oryzias latipes (japanese killifish) PHUM005326-PA Pediculus humanus (human body louse) ENSPPYP00000001676 Pongo pygmaeus (orangutan) ENSPTRP00000001091 Pan troglodytes (chimpanzee) ENSRNOP00000048402 Rattus norvegicus (norway rat) Smp_016340 Schistosoma mansoni (trematode parasite) XP_001179744.1 Strongylocentrotus purpuratus (purple sea urchin) 53928 Trichoplax adhaerens (metazoa) XP_973374.1 Tribolium castaneum (red flour beetle) ENSTNIP00000009954 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000042535 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000021128 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11237 LH16899 ISCW021461 FBgn0068533 FBgn0219847 FBgn0208921 FBgn0196592 FBgn0174687 JGI_V11_301758 FBgn0072629 FBgn0154425 FBgn0141515 FBgn0127523 FBgn0113051 FBgn0089705 CPIJ014300 BGIBMGA010622 ACYPI001064 GB20033 MED8_ANOGA MED8_AEDAE PB22017 PHUM504900 GLEAN_09839
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	34926 y1 sc* v1; P{TRiP.HMS01275}attP2 22510 y1 w67c23; P{EPgy2}MED8EY21803/CyO
VDRC	v107783 P{KK107827}VIE-260B v24806 w1118; P{GD8141}v24806 v24807 w1118; P{GD8141}v24807
Genomic Clones ( 2 )
	BACR10H12 BACR48L22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE16440
Other clones	FI07210
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH27118 SD27395
Other clones	81220 83505 CK01.26A.H11 CK01.27B.B12 Dmel_neb_015468_0819_2195 EK023640 EK024924 EK217648 UUGC0717
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034503 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37305
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Arc32 CG13867 (Boube et al., 2000) Source for identity of: MED8 Arc32 (Bourbon et al., 2004)
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_153772 RefSeq (Proteins) • NP_722456 DDBJ / EMBL / GenBank • AAL48730 AAM68406 AY071108 BI173508 Entrez Gene - A searchable database of RefSeq genes. • 37305 UniProtKB/TrEMBL • A1ZBT5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med8, fungi/metazoa (IPR019364)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62960 DroID - A comprehensive database of gene and protein interactions. • FBgn0034503 DRSC - Results from RNAi screens. • FBgn0034503 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034503 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13867-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034503 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37305 InParanoid A subset of ortholog calls from InParanoid. • AAEL010861-PA Aedes aegypti (mosquito) AGAP005909-PA Anopheles gambiae (mosquito) XP_623322.1 Apis mellifera (honey bee) XP_001949753.1 Acyrthosiphon pisum (pea aphid) 118497 Branchiostoma floridae (lancelet) 126473 Branchiostoma floridae (lancelet) BGIBMGA010622-PA Bombyx mori (silk worm) ENSBTAP00000022477 Bos taurus (bovine) CN19971 Caenorhabditis brenneri (small nematode) CE24544 Caenorhabditis elegans (worm) ENSCAFP00000007832 Canis familiaris (dog) ENSCINP00000011588 Ciona intestinalis (sea squirt) JA16572 Caenorhabditis japonica (nematode) ENSCPOP00000005502 Cavia porcellus (guinea pig) RP14671 Caenorhabditis remanei (nematode) ENSCSAVP00000011126 Ciona savignyi (sedentary tunicate) 226423 Capitella spl (polychaete worm) 301758 Daphnia pulex (daphnia) ENSDARP00000091089 Danio rerio (zebra fish) ENSECAP00000008464 Equus caballus (horse) ENSGACP00000005194 Gymnopilus aculeatus (mushroom) ENSGALP00000016218 Gallus gallus (chicken) 185934 Hypsipyla robusta (cedar shoot borer) ENSP00000290663 Homo sapiens (human) ISCW021461-PA Ixodes scapularis (deer tick) 186104 Lottia gigantea (owl limpet) ENSMODP00000014148 Monodelphis domestica (gray opossum) ENSMMUP00000031169 Macaca mulatta (rhesus monkey) ENSMUSP00000019229 Mus musculus (house mouse) 172778 Nematostella vectensis (starlet anemone) XP_001608087.1 Nasonia vitripennis (wasp) ENSOANP00000013051 Ornithorynchus anatinus (platypus) ENSORLP00000017797 Oryzias latipes (japanese killifish) PHUM005326-PA Pediculus humanus (human body louse) ENSPPYP00000001676 Pongo pygmaeus (orangutan) ENSPTRP00000001091 Pan troglodytes (chimpanzee) ENSRNOP00000048402 Rattus norvegicus (norway rat) Smp_016340 Schistosoma mansoni (trematode parasite) XP_001179744.1 Strongylocentrotus purpuratus (purple sea urchin) 53928 Trichoplax adhaerens (metazoa) XP_973374.1 Tribolium castaneum (red flour beetle) ENSTNIP00000009954 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000042535 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000021128 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37305 modMine - Data generated by the modENCODE project. • FBgn0034503 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11237 LH16899 ISCW021461 FBgn0068533 FBgn0219847 FBgn0208921 FBgn0196592 FBgn0174687 JGI_V11_301758 FBgn0072629 FBgn0154425 FBgn0141515 FBgn0127523 FBgn0113051 FBgn0089705 CPIJ014300 BGIBMGA010622 ACYPI001064 GB20033 MED8_ANOGA MED8_AEDAE PB22017 PHUM504900 GLEAN_09839
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	Arc32 (Bourbon et al., 2004, Guglielmi et al., 2004, Greene et al., 2005) ARC32 (Liebl et al., 2006) CG13867 (Mount, 2001.8.14, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) CG30140   dARC32 (Boube et al., 2000, Levine and Tjian, 2003) MED8 (Marr et al., 2006) Med8/ARC32 (Boube et al., 2002)
Name Synonym	Mediator complex subunit 8 (Bourbon et al., 2004)
Secondary FlyBase IDs
	FBgn0050140
References ( 20 )
Research paper	Bachtrog and Andolfatto, 2006, Genetics 174(4): 2045--2059 Selection, recombination and demographic history in Drosophila miranda. [FBrf0193451] Liebl et al., 2006, J. Neurobiol. 66(4): 332--347 Genome-wide P-element screen for Drosophila synaptogenesis mutants. [FBrf0191026] Marr et al., 2006, Genes Dev. 20(11): 1458--1469 Coactivator cross-talk specifies transcriptional output. [FBrf0192730] Greene et al., 2005, Hum. Molec. Genet. 14(6): 799--811 Genetic and genomic studies of Drosophila parkin mutants implicate oxidative stress and innate immune responses in pathogenesis. [FBrf0184023] Guglielmi et al., 2004, Nucleic Acids Res. 32(18): 5379--5391 A high resolution protein interaction map of the yeast Mediator complex. [FBrf0180597] Boube et al., 2000, Genes Dev. 14(22): 2906--2917 Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification. [FBrf0131257]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452] Oh et al., 2003, Genetics 163(1): Table S1. [FBrf0157097]
Review	Levine and Tjian, 2003, Nature 424: 147--151 Transcription regulation and animal diversity. [FBrf0185829] Boube et al., 2002, Cell 110(2): 143--151 Evidence for a mediator of RNA polymerase II transcriptional regulation conserved from yeast to man. [FBrf0151648]
Personal communication to FlyBase	Ni et al., 2011.7.19, TRiP germline vector pVALIUM21. TRiP germline vector pVALIUM21. [FBrf0214641] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bellen, 2006.8.3, MBBatch686_2.xml MBBatch686_2.xml [FBrf0191805] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Mount, 2001.8.14, Intron annotations. Intron annotations. [FBrf0137489]
Letter	Bourbon et al., 2004, Molec. Cell 14(5): 553--557 A unified nomenclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II. [FBrf0179142]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]