FlyBase Gene Report: Dmel\CG10543

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\CG10543 or the JBrowse view of Dmel\CG10543 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG10543-RA

FBtr0071524

NM_137690

7070

1634

CG10543-RD

FBtr0303195

NM_176241

5618

1631

CG10543-RE

FBtr0332593

NM_166416

5550

1634

CG10543-RF

FBtr0332594

NM_001274183

7073

1635

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG10543-PA

FBpp0071453

183.0

1634

6.51

NP_611534

AAF46652

CG10543-PD

FBpp0292294

182.6

1631

6.55

NP_788421

AAO41340

CG10543-PE

FBpp0304844

183.0

1634

6.51

NP_726015

AAM68188

CG10543-PF

FBpp0304845

183.1

1635

6.51

NP_001261112

AGB93644

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1634 aa isoforms: CG10543-PA, CG10543-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG10543 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

RNA polymerase II regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:P47043

(FlyBase Curators, 2008--)

sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

zinc ion binding

inferred from sequence or structural similarity with UniProtKB:P47043

(FlyBase Curators, 2008--)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

gravitaxis

inferred from mutant phenotype

(Armstrong et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:P47043

(FlyBase Curators, 2008--)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG10543 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG10543

View exonic expression by tissue for Dmel\CG10543

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034570

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034570

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10543-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10543

Flygut - An atlas of the Drosophila adult midgut

FBgn0034570

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG10543

Transgenic constructs containing regulatory region of CG10543

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)Exel6071

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | male limited, with Scer\GAL4^Bx-MS1096

CG10543^{UAS.ORF.GW.Tag:HA}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG10543^GD6647

viable, with Scer\GAL4^Mef2.PR

CG10543^GD6647

viable, with Scer\GAL4^pnr-MD237

CG10543^GD6647

Other Phenotypes

Allele

gravitaxis defective

CG10543^181Y

visible | female limited, with Scer\GAL4^Bx-MS1096

CG10543^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

wing | female limited, with Scer\GAL4^Bx-MS1096

CG10543^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ZNF697

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Zfp697

1 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\LOC102550892

1 of 13

Yes

Rnor\Zfp697

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (1)

Drer\si:dkey-226l10.6

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\C27A12.2

1 of 15

Yes

Cele\eor-1

1 of 15

Yes

Cele\unc-98

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902BS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\CG10543

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25089

Drosophila persimilis

Dper\GL10140

Drosophila willistoni

Dwil\GK22958

Drosophila virilis

Dvir\GJ21991

Drosophila mojavensis

Dmoj\GI21068

Drosophila grimshawi

Dgri\GH20522

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506YR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015006

Glossina morsitans

Tsetse fly

Gmor\GMOY007483

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13546

Mayetiola destructor

Hessian fly

Mdes\Mdes004495

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012168

Anopheles darlingi

American malaria mosquito

Adar\ADAC008910

Anopheles gambiae

Malaria mosquito

Agam\AGAP005135

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002267

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002269

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002268

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002265

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (33)

Gene Symbol

Score

Source

Alignment

az2

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

wor

1 of 10

zf30C

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63020

DroID - A comprehensive database of gene and protein interactions.

FBgn0034570

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37379

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-92

Cytogenetic map

57B5-57B5

Sequence location

2R:20,996,220..21,007,740 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57B5-57B5

Limits computationally determined from genome sequence between P{PZ}l(2)07806⁰⁷⁸⁰⁶ and P{EP}grau^EP688&P{PZ}shg¹⁰⁴⁶⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-92

(FlyBase, 2016)

2-99.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Bloomington

59049

w^*; P{UAS-CG10543.R}7A

59050

w^*; P{UAS-CG10543.R}3A

31964

y¹ v¹; P{TRiP.JF02977}attP2

54816

y¹ v¹; P{TRiP.HMJ21535}attP40

59992

y¹ w^*; PBac{CG10543-GFP.FPTB}VK00037

15047

y¹ w^67c23; P{EPgy2}CG10543^EY00916/CyO

83271

y¹ w^*; TI{CRIMIC.TG4.1}CG10543^{CR01331-TG4.1}/SM6a

FlyORF

F001853

M{UAS-CG10543.ORF.3xHA.GW}ZH-86Fb

Kyoto

103959

y^* w^*; P{GawB}CG10543^NP1253 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v103520

P{KK112630}VIE-260B

v30888

w¹¹¹⁸; P{GD6647}v30888

v342539

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02123}attP40/CyO-GFP

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (53)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

MIP26658

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034570

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034570

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37379

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37379

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034570

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034570

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10543

Flygut - An atlas of the Drosophila adult midgut

FBgn0034570

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37379

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10543

modMine - A data warehouse for the modENCODE project

FBgn0034570

Linkouts

BioGRID - A database of protein and genetic interactions.

63020

DroID - A comprehensive database of gene and protein interactions.

FBgn0034570

DRSC - Results frm RNAi screens

FBgn0034570

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034570

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10543-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034570

FlyMine - An integrated database for Drosophila genomics

FBgn0034570

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37379

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CG10543

(Gene Disruption Project members, 2018-, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Richardson, 2015.3.11, Schertel et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Tokusumi et al., 2012, Herranz et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Armstrong et al., 2006)

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (34)

Report Sections

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