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Gene Dmel\CG10306

General Information
Symbol	Dmel\CG10306	Species	D. melanogaster
Name		Annotation symbol	CG10306
Feature type	protein_coding_gene	FlyBase ID	FBgn0034654
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	57F8-57F8	Sequence location	2R:17,572,775..17,573,709 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG10306 (FBgn0034654). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: translation initiation factor activity. Based on sequence similarity, it is predicted to be involved in the biological process: translational initiation. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Armadillo-type fold; COP9 signalosome, subunit CSN8; Translation initiation factor 3, subunit 12, N-terminal, eukaryotic; Translation initiation factor 3, subunit 12, eukaryotic; Winged helix-turn-helix transcription repressor DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1635342_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG10306. Gene sequence location is 2R:17572775..17573709.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 20 of adulthood day 22 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 23 of adulthood day 35 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood day 34 of adulthood day 44 of adulthood day 14 of adulthood day 46 of adulthood
FB2012_01	Sequence features BKN22351 HFA04056
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 57F8-57F8   Limits computationally determined from genome sequence between P{EP}CG10082EP436&P{EP}EP712EP712 and P{EP}Tim10EP541&P{lacW}l(2)k09617k09617
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG10306 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG10306-RA FBtr0071670  NM_137760   869   222
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG10306-PA   FBpp0071587   25.7   222   6.40     NP_611604   AAF46753
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Translation initiation factor 3, subunit 12, eukaryotic (IPR009374) Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Translation initiation factor 3, subunit 12, N-terminal, eukaryotic (IPR016020) Armadillo-type fold (IPR016024) COP9 signalosome, subunit CSN8 (IPR019280)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263387   AA390301   AA735614   AA990794   AE013599 AAF46753   AI110013   AI297455   AI297666   AI389657   AI515530   AI544182   AI945850   AI946913   AY061068 AAL28616   AY231716   BF486289   BG632808   BI352466   BI353975   BI355420   BI564878   BI565451   BI571182   BI572812   BI575987   BI588342   BI589028   BI590928   BI591423   BI591629   BI591846   BI592421   BI605460   BI619159   BI621129   BI625012   BI625843   BI629649   BI633205   BI641607   BP540702   BP550075   BP551615   BP552508   BP555290   BP556642   BP559135   CK604484   CO153656   CO194811   CO282333   CO314540   CO314790   CO319789   EC062084   EC076826   EC204586   EC205657   EC215914   EC234225   EL875909   EV576149   EV578598   EV583088   EV585096   EV593678   GH943288   GH947736   GH948968   GH949912   GH949940   GH952164   GH952802   GH953999   GH954998   GH955923   GH957744   GH958515   GH959356   GH962111   GH962777   GH965532   GH966862   GH969873   GH970051   GH970640   GH972923   GH980452   GH983016   GH984145   GH985014
UniProtKB/Swiss-Prot	Q9W2D9
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0034654    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0034654 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0034654 expression level Developmental Stage   Expression Level embryo 00-02hr    17903 embryo 02-04hr    28257 embryo 04-06hr    29612 embryo 06-08hr    27336 embryo 08-10hr    27987 embryo 10-12hr    26655 embryo 12-14hr    16195 embryo 14-16hr    16761 embryo 16-18hr    12176 embryo 18-20hr    11268 embryo 20-22hr    10717 embryo 22-24hr    10112 larva L1    16928 larva L2    15277 larva L3 12hr old    10685 larva L3 puffstage 1-2    10419 larva L3 puffstage 3-6    11412 larva L3 puffstage 7-9    12220 white prepupae new    11842 white prepupae 12hr    12953 white prepupae 24hr    13800 pupae 2d postWPP    10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day    12851 adult female 05day    13440 adult female 30day    13696 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (17903) embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  (16195) embryo 14-16hr  (16761) embryo 16-18hr  (12176) embryo 18-20hr  (11268) embryo 20-22hr  (10717) embryo 22-24hr  (10112) larva L1  (16928) larva L2  (15277) larva L3 12hr old  (10685) larva L3 puffstage 1-2  (10419) larva L3 puffstage 3-6  (11412) larva L3 puffstage 7-9  (12220) white prepupae new  (11842) white prepupae 12hr  (12953) white prepupae 24hr  (13800) pupae 2d postWPP  (10150) pupae 3d postWPP  (6958) pupae 4d postWPP  (7596) adult male 01day  (8577) adult male 05day  (9758) adult male 30day  (9590) adult female 01day  (12851) adult female 05day  (13440) adult female 30day  (13696) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (17903) embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  (16195) embryo 14-16hr  (16761) embryo 16-18hr  (12176) embryo 18-20hr  (11268) embryo 20-22hr  (10717) embryo 22-24hr  (10112) larva L1  (16928) larva L2  (15277) larva L3 12hr old  (10685) larva L3 puffstage 1-2  (10419) larva L3 puffstage 3-6  (11412) larva L3 puffstage 7-9  (12220) white prepupae new  (11842) white prepupae 12hr  (12953) white prepupae 24hr  (13800) pupae 2d postWPP  (10150) pupae 3d postWPP  (6958) pupae 4d postWPP  (7596) adult male 01day  (8577) adult male 05day  (9758) adult male 30day  (9590) adult female 01day  (12851) adult female 05day  (13440) adult female 30day  (13696) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (17903) embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  (16195) embryo 14-16hr  (16761) embryo 16-18hr  (12176) embryo 18-20hr  (11268) embryo 20-22hr  10717 embryo 22-24hr  10112 larva L1  (16928) larva L2  (15277) larva L3 12hr old  10685 larva L3 puffstage 1-2  10419 larva L3 puffstage 3-6  (11412) larva L3 puffstage 7-9  (12220) white prepupae new  (11842) white prepupae 12hr  (12953) white prepupae 24hr  (13800) pupae 2d postWPP  10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day  (12851) adult female 05day  (13440) adult female 30day  (13696) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17903 embryo 02-04hr    28257 embryo 04-06hr    29612 embryo 06-08hr    27336 embryo 08-10hr    27987 embryo 10-12hr    26655 embryo 12-14hr    16195 embryo 14-16hr    16761 embryo 16-18hr    12176 embryo 18-20hr    11268 embryo 20-22hr    10717 embryo 22-24hr    10112 larva L1    16928 larva L2    15277 larva L3 12hr old    10685 larva L3 puffstage 1-2    10419 larva L3 puffstage 3-6    11412 larva L3 puffstage 7-9    12220 white prepupae new    11842 white prepupae 12hr    12953 white prepupae 24hr    13800 pupae 2d postWPP    10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day    12851 adult female 05day    13440 adult female 30day    13696 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0034654 expression level Developmental Stage   Expression Level embryo 00-02hr    17903 embryo 02-04hr    28257 embryo 04-06hr    29612 embryo 06-08hr    27336 embryo 08-10hr    27987 embryo 10-12hr    26655 embryo 12-14hr    16195 embryo 14-16hr    16761 embryo 16-18hr    12176 embryo 18-20hr    11268 embryo 20-22hr    10717 embryo 22-24hr    10112 larva L1    16928 larva L2    15277 larva L3 12hr old    10685 larva L3 puffstage 1-2    10419 larva L3 puffstage 3-6    11412 larva L3 puffstage 7-9    12220 white prepupae new    11842 white prepupae 12hr    12953 white prepupae 24hr    13800 pupae 2d postWPP    10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day    12851 adult female 05day    13440 adult female 30day    13696 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (17903) embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  (16195) embryo 14-16hr  (16761) embryo 16-18hr  (12176) embryo 18-20hr  (11268) embryo 20-22hr  (10717) embryo 22-24hr  (10112) larva L1  (16928) larva L2  (15277) larva L3 12hr old  (10685) larva L3 puffstage 1-2  (10419) larva L3 puffstage 3-6  (11412) larva L3 puffstage 7-9  (12220) white prepupae new  (11842) white prepupae 12hr  (12953) white prepupae 24hr  (13800) pupae 2d postWPP  (10150) pupae 3d postWPP  (6958) pupae 4d postWPP  (7596) adult male 01day  (8577) adult male 05day  (9758) adult male 30day  (9590) adult female 01day  (12851) adult female 05day  (13440) adult female 30day  (13696) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (17903) embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  (16195) embryo 14-16hr  (16761) embryo 16-18hr  (12176) embryo 18-20hr  (11268) embryo 20-22hr  (10717) embryo 22-24hr  (10112) larva L1  (16928) larva L2  (15277) larva L3 12hr old  (10685) larva L3 puffstage 1-2  (10419) larva L3 puffstage 3-6  (11412) larva L3 puffstage 7-9  (12220) white prepupae new  (11842) white prepupae 12hr  (12953) white prepupae 24hr  (13800) pupae 2d postWPP  (10150) pupae 3d postWPP  (6958) pupae 4d postWPP  (7596) adult male 01day  (8577) adult male 05day  (9758) adult male 30day  (9590) adult female 01day  (12851) adult female 05day  (13440) adult female 30day  (13696) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  17903 embryo 02-04hr  (28257) embryo 04-06hr  (29612) embryo 06-08hr  (27336) embryo 08-10hr  (27987) embryo 10-12hr  (26655) embryo 12-14hr  16195 embryo 14-16hr  16761 embryo 16-18hr  12176 embryo 18-20hr  11268 embryo 20-22hr  10717 embryo 22-24hr  10112 larva L1  16928 larva L2  15277 larva L3 12hr old  10685 larva L3 puffstage 1-2  10419 larva L3 puffstage 3-6  11412 larva L3 puffstage 7-9  12220 white prepupae new  11842 white prepupae 12hr  12953 white prepupae 24hr  13800 pupae 2d postWPP  10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day  12851 adult female 05day  13440 adult female 30day  13696 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17903 embryo 02-04hr    28257 embryo 04-06hr    29612 embryo 06-08hr    27336 embryo 08-10hr    27987 embryo 10-12hr    26655 embryo 12-14hr    16195 embryo 14-16hr    16761 embryo 16-18hr    12176 embryo 18-20hr    11268 embryo 20-22hr    10717 embryo 22-24hr    10112 larva L1    16928 larva L2    15277 larva L3 12hr old    10685 larva L3 puffstage 1-2    10419 larva L3 puffstage 3-6    11412 larva L3 puffstage 7-9    12220 white prepupae new    11842 white prepupae 12hr    12953 white prepupae 24hr    13800 pupae 2d postWPP    10150 pupae 3d postWPP    6958 pupae 4d postWPP    7596 adult male 01day    8577 adult male 05day    9758 adult male 30day    9590 adult female 01day    12851 adult female 05day    13440 adult female 30day    13696 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0034654    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0034654 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0034654 expression level Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland    1136.7 Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland    1652.7 Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland    2228.3 Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (738.175) Larval Midgut  (540.6) Larval Hindgut  (811.7) Larval Malpighian Tubules  (675.9) Larval Fat Body  (546.9) Larval Salivary Gland  (1136.7) Larval Trachea  (907.725) Larval Carcass  (613.2) Adult Head  (674.3) Adult Eye  (942.075) Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion  (741.1) Adult Crop  (573.7) Adult Midgut  (629.3) Adult Hindgut  (675.4) Adult Malpighian Tubules    270 Adult Fat Body  (897.9) Adult Salivary Gland  (1652.7) Adult Heart  (647.725) Adult VirginFemale Spermatheca  (837.6) Adult InseminatedFemale Spermatheca  (812.7) Adult Ovary  (691.3) Adult Testis    445.5 Adult Male Accessory Gland  (2228.3) Adult Carcass  (621.3) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland  (1136.7) Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland  (1652.7) Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland  (2228.3) Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland    1136.7 Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland    1652.7 Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland    2228.3 Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0034654 expression level Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland    1136.7 Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland    1652.7 Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland    2228.3 Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  738.175 Larval Midgut  540.6 Larval Hindgut  811.7 Larval Malpighian Tubules  675.9 Larval Fat Body  546.9 Larval Salivary Gland  (1136.7) Larval Trachea  (907.725) Larval Carcass  613.2 Adult Head  674.3 Adult Eye  (942.075) Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion  741.1 Adult Crop  573.7 Adult Midgut  629.3 Adult Hindgut  675.4 Adult Malpighian Tubules    270 Adult Fat Body  (897.9) Adult Salivary Gland  (1652.7) Adult Heart  647.725 Adult VirginFemale Spermatheca  (837.6) Adult InseminatedFemale Spermatheca  812.7 Adult Ovary  691.3 Adult Testis    445.5 Adult Male Accessory Gland  (2228.3) Adult Carcass  621.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland  1136.7 Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland  1652.7 Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland  (2228.3) Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    738.175 Larval Midgut    540.6 Larval Hindgut    811.7 Larval Malpighian Tubules    675.9 Larval Fat Body    546.9 Larval Salivary Gland    1136.7 Larval Trachea    907.725 Larval Carcass    613.2 Adult Head    674.3 Adult Eye    942.075 Adult Brain    499.9 Adult Thoracic-Abdominal Ganglion    741.1 Adult Crop    573.7 Adult Midgut    629.3 Adult Hindgut    675.4 Adult Malpighian Tubules    270 Adult Fat Body    897.9 Adult Salivary Gland    1652.7 Adult Heart    647.725 Adult VirginFemale Spermatheca    837.6 Adult InseminatedFemale Spermatheca    812.7 Adult Ovary    691.3 Adult Testis    445.5 Adult Male Accessory Gland    2228.3 Adult Carcass    621.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    674.3   NA  Eye    942.075   NA  Brain    499.9   738.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    741.1   NA  Crop    573.7   540.6  Midgut    629.3   811.7  Hindgut    675.4   675.9  Malpighian Tubules    270   546.9  Fat Body    897.9   1136.7  Salivary Gland    1652.7   NA  Heart    647.725   907.725  Trachea    NA   NA  VirginFemale Spermatheca    837.6   NA  InseminatedFemale Spermatheca    812.7   NA  Ovary    691.3   NA  Testis    445.5   NA  Male Accessory Gland    2228.3   613.2  Carcass    621.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034654 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10306-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG10306 Class Mutagen Stocks Known lesion CG10306HP25023 2 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG10306 Class Mutagen Stocks Known lesion CG10306KK102285 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG10306GD6934 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6934} NA P{KK102285} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPg}CG10306HP25023 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ribosome binding inferred from electronic annotation with InterPro:IPR016020 (FlyBase Curators et al., 2004-) translation initiation factor activity inferred from electronic annotation with InterPro:IPR009374, InterPro:IPR016020 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15)
Biological Process
CV term Evidence References
regulation of translational initiation inferred from electronic annotation with InterPro:IPR016020 (FlyBase Curators et al., 2004-) translational initiation inferred from sequence or structural similarity (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15)
Cellular Component
CV term Evidence References
eukaryotic translation initiation factor 3 complex inferred from electronic annotation with InterPro:IPR016020 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15) nucleus inferred from electronic annotation with InterPro:IPR009374 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG10306 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63100 DroID - A comprehensive database of gene and protein interactions. • FBgn0034654 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37475
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13261 Dere\GG22131 Dgri\GH22679 Dmoj\GI20605 Dper\GL10802 Dpse\GA24496 Dsec\GM15855 Dsim\GD11617 Dvir\GJ21045 Dwil\GK10740 Dyak\GE12213
Curated drosophilid orthologs	Dyak\GE12213
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006115-PA Aedes aegypti (mosquito) AGAP011580-PA Anopheles gambiae (mosquito) NP_001119623.1 Acyrthosiphon pisum (pea aphid) At4g33250.1 Arabidopsis thaliana (wall cress) 33890 Batrachochytrium dendrobatidis (chytrid fungus) 286987 Branchiostoma floridae (lancelet) BGIBMGA010671-PA Bombyx mori (silk worm) ENSBTAP00000014888 Bos taurus (bovine) CN14169 Caenorhabditis brenneri (small nematode) CBP16600 Caenorhabditis briggsae (nematode) CE20081 Caenorhabditis elegans (worm) ENSCAFP00000008778 Canis familiaris (dog) Chro.30453 Cryptosporidium hominis (obligate parasite) ENSCINP00000014937 Ciona intestinalis (sea squirt) JA04341 Caenorhabditis japonica (nematode) CMC106C Cyanidioschyzon merolae (red alga) cn04405 Cryptococcus neoformans (yeast-like fungus) cgd3_4020 Cryptosporidium parvum (protozoan parasite) CPIJ017839 Culex pipens (mosquito) ENSCPOP00000010703 Cavia porcellus (guinea pig) 140593 Chlamidomonas reinhardtii (green alga) RP39248 Caenorhabditis remanei (nematode) ENSCSAVP00000004984 Ciona savignyi (sedentary tunicate) 154858 Capitella spl (polychaete worm) DDB0234246 Dictyostelium discoideum (amoeba) 306983 Daphnia pulex (daphnia) ENSDARP00000043304 Danio rerio (zebra fish) ENSECAP00000010730 Equus caballus (horse) ENSGACP00000017406 Gymnopilus aculeatus (mushroom) 159094 Hypsipyla robusta (cedar shoot borer) ENSP00000248342 Homo sapiens (human) ISCW021577-PA Ixodes scapularis (deer tick) 194136 Lottia gigantea (owl limpet) 15993 Monocosta brevicollis (choanoflagellate) ENSMMUP00000031623 Macaca mulatta (rhesus monkey) ENSMUSP00000066038 Mus musculus (house mouse) 170558 Nematostella vectensis (starlet anemone) XP_001606859.1 Nasonia vitripennis (wasp) ENSOANP00000019299 Ornithorynchus anatinus (platypus) ENSORLP00000001194 Oryzias latipes (japanese killifish) LOC_Os03g08450.1 Oryza sativa (asian rice) PFC0441c Plasmodium falciparum (protozoal parasite) PHUM002032-PA Pediculus humanus (human body louse) 111962 Physcomitrella patens (moss) ENSPPYP00000023486 Pongo pygmaeus (orangutan) 82310 Phytophthora ramorum (sudden oak death pathogen) 136804 Phytophthora sojae (root rot pathogen) 739236 Populus trichocarpa (black cottonwood) ENSPTRP00000018746 Pan troglodytes (chimpanzee) PVX_119635 Plasmodium vivax (protozoal parasite) ENSRNOP00000027791 Rattus norvegicus (norway rat) 4745591 Sorghum bicolor (sorghum) Smp_099320 Schistosoma mansoni (trematode parasite) XP_792388.1 Strongylocentrotus purpuratus (purple sea urchin) 61914 Trichoplax adhaerens (metazoa) TA12425 Theileria annulata (apicomplexan parasite) XP_973326.1 Tribolium castaneum (red flour beetle) ENSTNIP00000009309 Tetraodon nigroviridis (green spotted pufferfish) TP02_0303 Theileria parva (parasitic protozoan) ENSTRUP00000023745 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000006037 Xenopus tropicalis (frog) YALI0D23243g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13467 LH17590 ISCW021577 FBgn0068513 FBgn0212752 FBgn0208179 FBgn0183357 FBgn0170771 JGI_V11_306983 FBgn0245887 FBgn0148412 FBgn0143340 FBgn0130137 FBgn0114305 FBgn0090293 CPIJ017839 BGIBMGA010671 ACYPI000023 GB18221 EIF3K_ANOGA AAEL006115 PB20244 PHUM464810 GLEAN_07178
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	21937 w1118; P{EPg}CG10306HP25023/CyO
Kyoto	114702 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EPg}CG10306HP25023 /CyO y+
VDRC	v105991 P{KK102285}VIE-260B
Genomic Clones ( 3 )
	BACR13F14 BACR34C17 BACR46G10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 88 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD03569
Other clones	yak-em_CG10306
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT20549 GH09438 GH14287 GH20885 GM19394 GM25795 GM30711 LD06230 LD21620 LD34675 LD47401 LD47634 LP11754 LP12021 RH04621 RH05194 RH06011 RH06381 RH07641 RH08454 RH10103 RH29523 RH30694 RH32427 RH50138 RH52427 RH58550 RH62420 RH63227 RH64818 RH66340 RH71070 SD25026 SD27224
Other clones	44843 50606 117719 176104 bs17e11 bs32h10 CK01.113C.A4 Dmel_dig_001534_0629_0438 Dmel_dig_003824_0683_3218 Dmel_dig_009869_0675_2018 Dmel_dig_009978_0710_2142 Dmel_neb_012504_0692_3147 EC39261 EK160238 EK236855 EK237808 EK280753 EN02525 FGA007C02 FGM105B07 FGM213H02 FGM219B07 FGM234E02 FGM304F12 FGM319B07 IP05810 RP004001842 RP004031444 RP004051098 RP004089079 RP004104841 RP004121155 RP004139327 RP004147838 RP004179020 RP004180191 RP004192780 RP004201645 RP004206977 RP004215198 RP004240659 RP004251458 RP004265225 RP004390330 RP004424069 RP004432208 RP004434264 RP004475115 RP004491139 RP004521588 RP004528255 UT01228
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034654 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37475
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137760 RefSeq (Proteins) • NP_611604 DDBJ / EMBL / GenBank • AA390301 AAF46753 AAL28616 AI297666 AI515530 AI945850 AI946913 AY061068 Entrez Gene - A searchable database of RefSeq genes. • 37475 UniProtKB/Swiss-Prot • Q9W2D9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Translation initiation factor 3, subunit 12, eukaryotic (IPR009374) Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Translation initiation factor 3, subunit 12, N-terminal, eukaryotic (IPR016020) Armadillo-type fold (IPR016024) COP9 signalosome, subunit CSN8 (IPR019280)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63100 DroID - A comprehensive database of gene and protein interactions. • FBgn0034654 DRSC - Results from RNAi screens. • FBgn0034654 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034654 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10306-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034654 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37475 InParanoid A subset of ortholog calls from InParanoid. • AAEL006115-PA Aedes aegypti (mosquito) AGAP011580-PA Anopheles gambiae (mosquito) NP_001119623.1 Acyrthosiphon pisum (pea aphid) At4g33250.1 Arabidopsis thaliana (wall cress) 33890 Batrachochytrium dendrobatidis (chytrid fungus) 286987 Branchiostoma floridae (lancelet) BGIBMGA010671-PA Bombyx mori (silk worm) ENSBTAP00000014888 Bos taurus (bovine) CN14169 Caenorhabditis brenneri (small nematode) CBP16600 Caenorhabditis briggsae (nematode) CE20081 Caenorhabditis elegans (worm) ENSCAFP00000008778 Canis familiaris (dog) Chro.30453 Cryptosporidium hominis (obligate parasite) ENSCINP00000014937 Ciona intestinalis (sea squirt) JA04341 Caenorhabditis japonica (nematode) CMC106C Cyanidioschyzon merolae (red alga) cn04405 Cryptococcus neoformans (yeast-like fungus) cgd3_4020 Cryptosporidium parvum (protozoan parasite) CPIJ017839 Culex pipens (mosquito) ENSCPOP00000010703 Cavia porcellus (guinea pig) 140593 Chlamidomonas reinhardtii (green alga) RP39248 Caenorhabditis remanei (nematode) ENSCSAVP00000004984 Ciona savignyi (sedentary tunicate) 154858 Capitella spl (polychaete worm) DDB0234246 Dictyostelium discoideum (amoeba) 306983 Daphnia pulex (daphnia) ENSDARP00000043304 Danio rerio (zebra fish) ENSECAP00000010730 Equus caballus (horse) ENSGACP00000017406 Gymnopilus aculeatus (mushroom) 159094 Hypsipyla robusta (cedar shoot borer) ENSP00000248342 Homo sapiens (human) ISCW021577-PA Ixodes scapularis (deer tick) 194136 Lottia gigantea (owl limpet) 15993 Monocosta brevicollis (choanoflagellate) ENSMMUP00000031623 Macaca mulatta (rhesus monkey) ENSMUSP00000066038 Mus musculus (house mouse) 170558 Nematostella vectensis (starlet anemone) XP_001606859.1 Nasonia vitripennis (wasp) ENSOANP00000019299 Ornithorynchus anatinus (platypus) ENSORLP00000001194 Oryzias latipes (japanese killifish) LOC_Os03g08450.1 Oryza sativa (asian rice) PFC0441c Plasmodium falciparum (protozoal parasite) PHUM002032-PA Pediculus humanus (human body louse) 111962 Physcomitrella patens (moss) ENSPPYP00000023486 Pongo pygmaeus (orangutan) 82310 Phytophthora ramorum (sudden oak death pathogen) 136804 Phytophthora sojae (root rot pathogen) 739236 Populus trichocarpa (black cottonwood) ENSPTRP00000018746 Pan troglodytes (chimpanzee) PVX_119635 Plasmodium vivax (protozoal parasite) ENSRNOP00000027791 Rattus norvegicus (norway rat) 4745591 Sorghum bicolor (sorghum) Smp_099320 Schistosoma mansoni (trematode parasite) XP_792388.1 Strongylocentrotus purpuratus (purple sea urchin) 61914 Trichoplax adhaerens (metazoa) TA12425 Theileria annulata (apicomplexan parasite) XP_973326.1 Tribolium castaneum (red flour beetle) ENSTNIP00000009309 Tetraodon nigroviridis (green spotted pufferfish) TP02_0303 Theileria parva (parasitic protozoan) ENSTRUP00000023745 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000006037 Xenopus tropicalis (frog) YALI0D23243g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37475 modMine - Data generated by the modENCODE project. • FBgn0034654 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13467 LH17590 ISCW021577 FBgn0068513 FBgn0212752 FBgn0208179 FBgn0183357 FBgn0170771 JGI_V11_306983 FBgn0245887 FBgn0148412 FBgn0143340 FBgn0130137 FBgn0114305 FBgn0090293 CPIJ017839 BGIBMGA010671 ACYPI000023 GB18221 EIF3K_ANOGA AAEL006115 PB20244 PHUM464810 GLEAN_07178
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CG10306 (Stuart et al., 2007, Keleman et al., 2009.8.5, Gandhi et al., 2007, Cammarato et al., 2011)
Name Synonym
Secondary FlyBase IDs
References ( 15 )
Research paper	Stuart et al., 2007, Nature 445(7123): 95--101 A systems biology analysis of the Drosophila phagosome. [FBrf0195342] Pile et al., 2003, J. Biol. Chem. 278(39): 37840--37848 The SIN3 deacetylase complex represses genes encoding mitochondrial proteins: Implications for the regulation of energy metabolism. [FBrf0162185]
Supplementary material	Cammarato et al., 2011, PLoS ONE 6(4): Table S1. Proteins & Peptides. [FBrf0214682] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Dietzl et al., 2007, Nature 448(7150): Supplementary Table 1. [FBrf0202579] Gandhi et al., 2007, Nat. Genet. 38(3): Supplementary Information [FBrf0208882] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Levis, 2009.8.13, Release 5 sequence localizations for HP and DP lines. Release 5 sequence localizations for HP and DP lines. [FBrf0208533] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Oliver, 2001.8.16, File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Protein sequence record	Swiss-Prot Project Members, 2002.6.15, UniProtKB: Q9W2D9 Probable eukaryotic translation initiation factor 3 subunit 11 (eIF-3 p25) (eIF3k). [FBrf0156985]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]