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Gene Dmel\Vps35

General Information
Symbol	Dmel\Vps35	Species	D. melanogaster
Name	Vacuolar protein sorting 35	Annotation symbol	CG5625
Feature type	protein_coding_gene	FlyBase ID	FBgn0034708
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	Dvps35
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	58C7-58C7	Sequence location	2R:18,073,483..18,077,392 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vacuolar protein sorting 35 is referred to in FlyBase by the symbol Dmel\Vps35 (CG5625, FBgn0034708). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: endocytosis. 9 alleles are reported. The phenotypes of these alleles are annotated with: NMJ bouton; lamellocyte. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Vacuolar protein sorting-associated protein 35. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625656_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Vps35. Gene sequence location is 2R:18073483..18077392.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 20 of adulthood day 22 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 23 of adulthood day 35 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood day 34 of adulthood day 44 of adulthood day 14 of adulthood day 46 of adulthood
FB2012_01	Sequence features HFA04519 BKN20096
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 58C7-58C7   Limits computationally determined from genome sequence between P{EP}CG6393EP2457 and P{PZ}ari-207768
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vps35 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone LD15794 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vps35-RA FBtr0071772  NM_137807   3138   803 Vps35-RB FBtr0071771  NM_166518   3364   822
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vps35-PA   FBpp0071686   91.1   803   5.09     NP_611651   AAF46816 Vps35-PB   FBpp0071685   93.1   822   5.14     NP_726175   AAF46817
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Vacuolar protein sorting-associated protein 35 (IPR005378)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391009   AA391150   AA539459   AA950018   AE013599 AAF46816   AE013599 AAF46817   AI110053   AI402179   AI517517   AW942454   AY061234 AAL28782   BF506518   BI164048   BI230580   BI237580   BI238554   BI356582   BI361492   BI363496   BI366552   BI367017   BI367322   BI367397   BI370154   BI375736   BI482349   BI570751   BI570896   BI571154   BI571264   BI571955   BI580456   BI591760   BI640812   BP552580   BT015203   BT100312   CL943614   CO191990   CO264385   CO271516   CO276180   CO281850   CO283176   CO287703   CO293996   CO296447   CO307080   CO314010   CO316161   CO317357   CO317425   CO318830   CO341399   CO342865   DN154301   EC055137   EC066614   EC066615   EC075533   EC076790   EC197606   EC202857   EC204527   EC205091   EC209764   EC212725   EC213048   EC214872   EC218894   EC222747   EC234278   EC250173   EC250397   EC252350   EC258276   EV588186   EV600550   GH757923   GH758280   GH758342   GH758383   GH759785   GH773217   GH773580   GH777190   GH777447   GH777570   GH778175   GH786981   GH788398   GH789406   GH791324   GH791705   GH792079   GH793274   GH795934   GH799598   GH800631   GH801382   GH805060   GH807995   GH810557   GH811082   GH812144   GH814359   GH822566   GH823064   GH828351   GH829173   GH836860   GH838406   GH843755   GH847080   GH847132   GH849466   GH852591   GH857038   GH861907   GH864020   GH868264   GH877454   GH878863   GH878987   GH880150   GH880366   GH889630   GH894191   GH894958   GH897943   GH898827   GH904065   GH904095   GH904928   GH904945   GH909245   GH914326   GH918762   GH920154   GH922000   GH922134   GH922533   GH923846   GH923863   GH925467   GH926233   GH928144   GH928428   GH928580   GH930356   GH931194   GH933885   GH934266   GH934347   GH939772   GH940704
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KVL7 Q95RP4 Q9W277
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0034708    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0034708 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0034708 expression level Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3962) embryo 02-04hr  (4492) embryo 04-06hr  (3366) embryo 06-08hr  (3011) embryo 08-10hr  (3551) embryo 10-12hr  (3041) embryo 12-14hr  (2930) embryo 14-16hr  (2521) embryo 16-18hr  (2066) embryo 18-20hr  (1443) embryo 20-22hr  (1918) embryo 22-24hr  (2041) larva L1  (1847) larva L2  (1922) larva L3 12hr old  (1441) larva L3 puffstage 1-2  (1445) larva L3 puffstage 3-6  (1988) larva L3 puffstage 7-9  (2492) white prepupae new  (2681) white prepupae 12hr  (2264) white prepupae 24hr  (2623) pupae 2d postWPP  (2259) pupae 3d postWPP  (1720) pupae 4d postWPP  (1353) adult male 01day  (1885) adult male 05day  (2028) adult male 30day  (1931) adult female 01day  (2220) adult female 05day  (3119) adult female 30day  (2868) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3962) embryo 02-04hr  (4492) embryo 04-06hr  (3366) embryo 06-08hr  (3011) embryo 08-10hr  (3551) embryo 10-12hr  (3041) embryo 12-14hr  (2930) embryo 14-16hr  (2521) embryo 16-18hr  (2066) embryo 18-20hr  1443 embryo 20-22hr  (1918) embryo 22-24hr  (2041) larva L1  (1847) larva L2  (1922) larva L3 12hr old  1441 larva L3 puffstage 1-2  1445 larva L3 puffstage 3-6  (1988) larva L3 puffstage 7-9  (2492) white prepupae new  (2681) white prepupae 12hr  (2264) white prepupae 24hr  (2623) pupae 2d postWPP  (2259) pupae 3d postWPP  (1720) pupae 4d postWPP    1353 adult male 01day  (1885) adult male 05day  (2028) adult male 30day  (1931) adult female 01day  (2220) adult female 05day  (3119) adult female 30day  (2868) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0034708 expression level Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3962) embryo 02-04hr  (4492) embryo 04-06hr  (3366) embryo 06-08hr  (3011) embryo 08-10hr  (3551) embryo 10-12hr  (3041) embryo 12-14hr  (2930) embryo 14-16hr  (2521) embryo 16-18hr  (2066) embryo 18-20hr  (1443) embryo 20-22hr  (1918) embryo 22-24hr  (2041) larva L1  (1847) larva L2  (1922) larva L3 12hr old  (1441) larva L3 puffstage 1-2  (1445) larva L3 puffstage 3-6  (1988) larva L3 puffstage 7-9  (2492) white prepupae new  (2681) white prepupae 12hr  (2264) white prepupae 24hr  (2623) pupae 2d postWPP  (2259) pupae 3d postWPP  (1720) pupae 4d postWPP  (1353) adult male 01day  (1885) adult male 05day  (2028) adult male 30day  (1931) adult female 01day  (2220) adult female 05day  (3119) adult female 30day  (2868) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3962) embryo 02-04hr  (4492) embryo 04-06hr  (3366) embryo 06-08hr  (3011) embryo 08-10hr  (3551) embryo 10-12hr  (3041) embryo 12-14hr  (2930) embryo 14-16hr  (2521) embryo 16-18hr  2066 embryo 18-20hr  1443 embryo 20-22hr  1918 embryo 22-24hr  2041 larva L1  1847 larva L2  1922 larva L3 12hr old  1441 larva L3 puffstage 1-2  1445 larva L3 puffstage 3-6  1988 larva L3 puffstage 7-9  (2492) white prepupae new  (2681) white prepupae 12hr  2264 white prepupae 24hr  (2623) pupae 2d postWPP  2259 pupae 3d postWPP  1720 pupae 4d postWPP    1353 adult male 01day  1885 adult male 05day  2028 adult male 30day  1931 adult female 01day  2220 adult female 05day  (3119) adult female 30day  (2868) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3962 embryo 02-04hr    4492 embryo 04-06hr    3366 embryo 06-08hr    3011 embryo 08-10hr    3551 embryo 10-12hr    3041 embryo 12-14hr    2930 embryo 14-16hr    2521 embryo 16-18hr    2066 embryo 18-20hr    1443 embryo 20-22hr    1918 embryo 22-24hr    2041 larva L1    1847 larva L2    1922 larva L3 12hr old    1441 larva L3 puffstage 1-2    1445 larva L3 puffstage 3-6    1988 larva L3 puffstage 7-9    2492 white prepupae new    2681 white prepupae 12hr    2264 white prepupae 24hr    2623 pupae 2d postWPP    2259 pupae 3d postWPP    1720 pupae 4d postWPP    1353 adult male 01day    1885 adult male 05day    2028 adult male 30day    1931 adult female 01day    2220 adult female 05day    3119 adult female 30day    2868 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0034708    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0034708 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0034708 expression level Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0034708 expression level Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    444.5 Larval Midgut    276.6 Larval Hindgut    382.4 Larval Malpighian Tubules    455.8 Larval Fat Body    288.1 Larval Salivary Gland    492.5 Larval Trachea    342.575 Larval Carcass    305.875 Adult Head    288.5 Adult Eye    393.625 Adult Brain    304.7 Adult Thoracic-Abdominal Ganglion    312.9 Adult Crop    349.3 Adult Midgut    330.7 Adult Hindgut    311.7 Adult Malpighian Tubules    430.8 Adult Fat Body    272 Adult Salivary Gland    433.3 Adult Heart    342.55 Adult VirginFemale Spermatheca    300.3 Adult InseminatedFemale Spermatheca    331.5 Adult Ovary    362.7 Adult Testis    97.1 Adult Male Accessory Gland    364.8 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    288.5   NA  Eye    393.625   NA  Brain    304.7   444.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    312.9   NA  Crop    349.3   276.6  Midgut    330.7   382.4  Hindgut    311.7   455.8  Malpighian Tubules    430.8   288.1  Fat Body    272   492.5  Salivary Gland    433.3   NA  Heart    342.55   342.575  Trachea    NA   NA  VirginFemale Spermatheca    300.3   NA  InseminatedFemale Spermatheca    331.5   NA  Ovary    362.7   NA  Testis    97.1   NA  Male Accessory Gland    364.8   305.875  Carcass    236.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034708 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5625-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2R)Exel6078) Vps35MH20 lethal | embryonic stage | maternal effect | recessive Vps35MH20 lethal | larval stage (with Df(2R)ED3952) Vps35EY14200 lethal | larval stage | recessive Vps35EY14200 lethal | prepupal stage | recessive Vps35MH20 lethal | pupal stage (with Df(2R)ED3952) Vps35EY14200 lethal | pupal stage | recessive Vps35EY14200 lethal | recessive Vps35EY14200 lethal | second instar larval stage Vps35E42 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Vps35GD11710 Other Phenotypes Allele melanotic mass phenotype | larval stage Vps35EY14200 Vps35MH20 melanotic mass phenotype | larval stage (with Df(2R)Exel6078) Vps35MH20 neuroanatomy defective | larval stage Vps35EY14200 neuroanatomy defective | larval stage (with Df(2R)ED3952) Vps35EY14200 visible | somatic clone Vps35MH20 Phenotype manifest in Allele embryonic/larval hemocyte | supernumerary (with Df(2R)ED3952) Vps35EY14200 follicle cell | somatic clone Vps35MH20 lamellocyte Vps35EY14200 melanotic mass | larval stage Vps35EY14200 Vps35MH20 NMJ bouton Vps35EY14200 NMJ bouton (with Df(2R)ED3952) Vps35EY14200 plasmatocyte | supernumerary Vps35EY14200 wing margin | somatic clone Vps35MH20
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of Vps35 Class Mutagen Stocks Known lesion Vps35EY14200 P-element activity 2 Yes Vps35DG08107 1 -- Vps35EY16641 P-element activity 1 -- Vps351 P-element activity 0 Yes Vps35E42 P-element activity 0 Yes Vps35MH20 loss of function allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vps35 Class Mutagen Stocks Known lesion Vps35GD11710 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Vps35dsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Vps35Scer\UAS.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6078 (Ryder, 2004.4.26, Franch-Marro et al., 2008) Df(2R)Exel7171 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Vps35EY14200 NA P{EPgy2}Vps35EY16641 NA miscellaneous insertions P{wHy}Vps35DG08107 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
endocytosis inferred from mutant phenotype (Korolchuk et al., 2007)
Cellular Component
CV term Evidence References
endosome inferred from direct assay (Korolchuk et al., 2007)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
vesicle-mediated transport inferred from sequence or structural similarity with Saccharomyces VPS35 (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vps35 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63153 DroID - A comprehensive database of gene and protein interactions. • FBgn0034708 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37536
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12616 Dere\GG22173 Dgri\GH21205 Dmoj\GI20494 Dper\GL17528 Dpse\GA24193 Dsec\GM15895 Dsim\GD11652 Dvir\GJ22344 Dwil\GK20803 Dyak\GE14166
Curated drosophilid orthologs	Dpse\GA19013
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013386-PA Aedes aegypti (mosquito) AGAP007683-PA Anopheles gambiae (mosquito) XP_392327.2 Apis mellifera (honey bee) XP_001951013.1 Acyrthosiphon pisum (pea aphid) At3g51310.1 Arabidopsis thaliana (wall cress) 693 Batrachochytrium dendrobatidis (chytrid fungus) 282362 Branchiostoma floridae (lancelet) 284676 Branchiostoma floridae (lancelet) BGIBMGA014194-PA Bombyx mori (silk worm) ENSBTAP00000003239 Bos taurus (bovine) CAL0005872 Candida albicans (a form of yeast) CBP33388 Caenorhabditis briggsae (nematode) CE39945 Caenorhabditis elegans (worm) ENSCAFP00000005503 Canis familiaris (dog) CAGL0I06072g Candida glabrata (haploid yeast) Chro.40270 Cryptosporidium hominis (obligate parasite) ENSCINP00000000530 Ciona intestinalis (sea squirt) JA02550 Caenorhabditis japonica (nematode) CMH220C Cyanidioschyzon merolae (red alga) cn03402 Cryptococcus neoformans (yeast-like fungus) cgd4_2390 Cryptosporidium parvum (protozoan parasite) CPIJ018730 Culex pipens (mosquito) ENSCPOP00000003407 Cavia porcellus (guinea pig) RP30481 Caenorhabditis remanei (nematode) ENSCSAVP00000018429 Ciona savignyi (sedentary tunicate) 161426 Capitella spl (polychaete worm) DDB0234193 Dictyostelium discoideum (amoeba) DEHA2C15312g Debaryomyces Hansenii (yeast) 315698 Daphnia pulex (daphnia) ENSDARP00000003960 Danio rerio (zebra fish) ENSECAP00000003443 Equus caballus (horse) XP_652967 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018758 Gymnopilus aculeatus (mushroom) ENSGALP00000006965 Gallus gallus (chicken) GL50803_23833 Giardia lamblia (flagellated protozoan) 96873 Hypsipyla robusta (cedar shoot borer) ENSP00000299138 Homo sapiens (human) ISCW014873-PA Ixodes scapularis (deer tick) KLLA0B07535g Kluyveromyces lactis (yeast) 206542 Lottia gigantea (owl limpet) LmjF21.0470 Leishmania major (virus) 28281 Monocosta brevicollis (choanoflagellate) ENSMODP00000015995 Monodelphis domestica (gray opossum) ENSMMUP00000015659 Macaca mulatta (rhesus monkey) ENSMUSP00000034131 Mus musculus (house mouse) 161846 Nematostella vectensis (starlet anemone) XP_001604419.1 Nasonia vitripennis (wasp) ENSOANP00000003564 Ornithorynchus anatinus (platypus) ENSORLP00000025144 Oryzias latipes (japanese killifish) LOC_Os03g58700.1 Oryza sativa (asian rice) 754 Ostreococcus tauri (green alga) PF11_0112 Plasmodium falciparum (protozoal parasite) PHUM001899-PA Pediculus humanus (human body louse) PP19262 Pristionchus pacificus (nematode) 171851 Physcomitrella patens (moss) ENSPPYP00000008717 Pongo pygmaeus (orangutan) 83835 Phytophthora ramorum (sudden oak death pathogen) 128134 Phytophthora sojae (root rot pathogen) 801569 Populus trichocarpa (black cottonwood) ENSPTRP00000013794 Pan troglodytes (chimpanzee) PVX_091175 Plasmodium vivax (protozoal parasite) ENSRNOP00000024020 Rattus norvegicus (norway rat) 141361 Sorghum bicolor (sorghum) YJL154C Saccharomyces cerevisiae (budding yeast) Smp_173330 Schistosoma mansoni (trematode parasite) SPCC777.13 Schizosaccharomyces pombe (fission yeast) XP_001190766.1 Strongylocentrotus purpuratus (purple sea urchin) 30598 Trichoplax adhaerens (metazoa) TA05695 Theileria annulata (apicomplexan parasite) XP_967674.1 Tribolium castaneum (red flour beetle) Tc00.1047053511367.170 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000007663 Tetraodon nigroviridis (green spotted pufferfish) TP01_0794 Theileria parva (parasitic protozoan) 36337 Thalassiosira pseudonana (marine diatom) ENSTRUP00000002580 Takifugu rubripes (pufferfish Takifugu) 3692.m00085 Tetrahymena thermophila (ciliate protozoa) 87500.m00060 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000008435 Xenopus tropicalis (frog) YALI0E17413g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12450 LH13587 ISCW014873 FBgn0231788 FBgn0222799 FBgn0209457 FBgn0183392 FBgn0170811 JGI_V11_315698 FBgn0245592 FBgn0155131 FBgn0143230 FBgn0128667 FBgn0114347 FBgn0089652 CPIJ018730 BGIBMGA014194 ACYPI008757 GB15658 AGAP007683 AAEL013386 PB25194 PHUM550160 GLEAN_08602
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	21642 y1 w67c23; P{wHy}Vps35DG08107 20913 y1 w67c23; P{EPgy2}Vps35EY14200/CyO 22327 y1 w67c23; P{EPgy2}Vps35EY16641/SM6a
Kyoto	114684 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}Vps35EY14200 /CyO y+
VDRC	v22180 w1118 P{GD11710}v22180 v45570 w1118; P{GD11710}v45570
Genomic Clones ( 1 )
	BACR02O12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 148 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD17594 RE65032 SD03023
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT09011 GH28513 LD09234 LD09906 LD29957 RE03618 RE14284 RE33829 RE35043 RE42761 RE45724 RE48319 RE52117 RE52682 RE53064 RE53158 RE56568 RE63606 RE74652 RH05528 RH05682 RH05977 RH06106 RH06738 RH07277 SD23815
Other clones	30019 51474 75563 78116 80413 109055 109321 148129 163304 163318 172804 175143 175757 211074.1 214445.63 214447.64 CK01.83C.A12 CK01.168D.H11 Dmel_neb_012033_0822_2195 Dmel_neb_012594_0162_0291 EC32540 EC055137 EK066536 EK103730 EK119902 EK138923 EK158929 EK162430 EK205341 EK211419 EK213657 EK234709 EK253460 EK263824 EK267763 EK267962 EK274334 EP12363 EP15639 FGM219E12 GH09501 RP003033526 RP003055165 RP003080343 RP003080705 RP003084994 RP003086011 RP003093025 RP003095611 RP003097994 RP003098456 RP003111330 RP003114937 RP003148624 RP003150533 RP003157735 RP003178279 RP003206113 RP003207993 RP003211956 RP003221222 RP003234374 RP003235115 RP003252413 RP003267625 RP003277382 RP003288586 RP003290419 RP003299076 RP003299678 RP003301865 RP003320650 RP003322221 RP003327893 RP003333714 RP003336670 RP003337678 RP003347435 RP003353946 RP003368384 RP003372296 RP003385708 RP003390982 RP003391371 RP003400788 RP003402020 RP003402309 RP003415824 RP003422594 RP003426017 RP003426312 RP003450803 RP003454216 RP003462847 RP003468890 RP003489086 RP003500176 RP003500770 RP003505287 RP003513168 RP003517757 RP003519390 RP003524214 RP003524633 RP003555054 RP003555400 RP003568504 RP003587609 RP003618205 RP003627482 RP003628871 RP003629948 RP003631287 RP003640848 RP003640989 RP003647639 RP003652573 RP003663581 RP003670983
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034708 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37536
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Vps35 CG5625 (Korolchuk et al., 2007)
Source for database merge of
Additional comments	FlyBase curator comment: Correspondence with author Lin revealed that 'Vps35' = 'CG5625'. (Lin et al., 2008)
Other Comments
	Vps35 is required for efficient progress of wg protein through the secretory pathway. (Franch-Marro et al., 2008) Vps35 is required for secretion of wg protein. (Port et al., 2008)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137807 NM_166518 RefSeq (Proteins) • NP_611651 NP_726175 DDBJ / EMBL / GenBank • AA539459 AAF46816 AAF46817 AAL28782 AW942454 AY061234 BI482349 CL943614 Entrez Gene - A searchable database of RefSeq genes. • 37536 UniProtKB/TrEMBL • Q7KVL7 Q9W277 Q95RP4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Vacuolar protein sorting-associated protein 35 (IPR005378)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63153 DroID - A comprehensive database of gene and protein interactions. • FBgn0034708 DRSC - Results from RNAi screens. • FBgn0034708 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034708 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5625-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034708 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37536 InParanoid A subset of ortholog calls from InParanoid. • AAEL013386-PA Aedes aegypti (mosquito) AGAP007683-PA Anopheles gambiae (mosquito) XP_392327.2 Apis mellifera (honey bee) XP_001951013.1 Acyrthosiphon pisum (pea aphid) At3g51310.1 Arabidopsis thaliana (wall cress) 693 Batrachochytrium dendrobatidis (chytrid fungus) 282362 Branchiostoma floridae (lancelet) 284676 Branchiostoma floridae (lancelet) BGIBMGA014194-PA Bombyx mori (silk worm) ENSBTAP00000003239 Bos taurus (bovine) CAL0005872 Candida albicans (a form of yeast) CBP33388 Caenorhabditis briggsae (nematode) CE39945 Caenorhabditis elegans (worm) ENSCAFP00000005503 Canis familiaris (dog) CAGL0I06072g Candida glabrata (haploid yeast) Chro.40270 Cryptosporidium hominis (obligate parasite) ENSCINP00000000530 Ciona intestinalis (sea squirt) JA02550 Caenorhabditis japonica (nematode) CMH220C Cyanidioschyzon merolae (red alga) cn03402 Cryptococcus neoformans (yeast-like fungus) cgd4_2390 Cryptosporidium parvum (protozoan parasite) CPIJ018730 Culex pipens (mosquito) ENSCPOP00000003407 Cavia porcellus (guinea pig) RP30481 Caenorhabditis remanei (nematode) ENSCSAVP00000018429 Ciona savignyi (sedentary tunicate) 161426 Capitella spl (polychaete worm) DDB0234193 Dictyostelium discoideum (amoeba) DEHA2C15312g Debaryomyces Hansenii (yeast) 315698 Daphnia pulex (daphnia) ENSDARP00000003960 Danio rerio (zebra fish) ENSECAP00000003443 Equus caballus (horse) XP_652967 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018758 Gymnopilus aculeatus (mushroom) ENSGALP00000006965 Gallus gallus (chicken) GL50803_23833 Giardia lamblia (flagellated protozoan) 96873 Hypsipyla robusta (cedar shoot borer) ENSP00000299138 Homo sapiens (human) ISCW014873-PA Ixodes scapularis (deer tick) KLLA0B07535g Kluyveromyces lactis (yeast) 206542 Lottia gigantea (owl limpet) LmjF21.0470 Leishmania major (virus) 28281 Monocosta brevicollis (choanoflagellate) ENSMODP00000015995 Monodelphis domestica (gray opossum) ENSMMUP00000015659 Macaca mulatta (rhesus monkey) ENSMUSP00000034131 Mus musculus (house mouse) 161846 Nematostella vectensis (starlet anemone) XP_001604419.1 Nasonia vitripennis (wasp) ENSOANP00000003564 Ornithorynchus anatinus (platypus) ENSORLP00000025144 Oryzias latipes (japanese killifish) LOC_Os03g58700.1 Oryza sativa (asian rice) 754 Ostreococcus tauri (green alga) PF11_0112 Plasmodium falciparum (protozoal parasite) PHUM001899-PA Pediculus humanus (human body louse) PP19262 Pristionchus pacificus (nematode) 171851 Physcomitrella patens (moss) ENSPPYP00000008717 Pongo pygmaeus (orangutan) 83835 Phytophthora ramorum (sudden oak death pathogen) 128134 Phytophthora sojae (root rot pathogen) 801569 Populus trichocarpa (black cottonwood) ENSPTRP00000013794 Pan troglodytes (chimpanzee) PVX_091175 Plasmodium vivax (protozoal parasite) ENSRNOP00000024020 Rattus norvegicus (norway rat) 141361 Sorghum bicolor (sorghum) YJL154C Saccharomyces cerevisiae (budding yeast) Smp_173330 Schistosoma mansoni (trematode parasite) SPCC777.13 Schizosaccharomyces pombe (fission yeast) XP_001190766.1 Strongylocentrotus purpuratus (purple sea urchin) 30598 Trichoplax adhaerens (metazoa) TA05695 Theileria annulata (apicomplexan parasite) XP_967674.1 Tribolium castaneum (red flour beetle) Tc00.1047053511367.170 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000007663 Tetraodon nigroviridis (green spotted pufferfish) TP01_0794 Theileria parva (parasitic protozoan) 36337 Thalassiosira pseudonana (marine diatom) ENSTRUP00000002580 Takifugu rubripes (pufferfish Takifugu) 3692.m00085 Tetrahymena thermophila (ciliate protozoa) 87500.m00060 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000008435 Xenopus tropicalis (frog) YALI0E17413g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37536 modMine - Data generated by the modENCODE project. • FBgn0034708 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12450 LH13587 ISCW014873 FBgn0231788 FBgn0222799 FBgn0209457 FBgn0183392 FBgn0170811 JGI_V11_315698 FBgn0245592 FBgn0155131 FBgn0143230 FBgn0128667 FBgn0114347 FBgn0089652 CPIJ018730 BGIBMGA014194 ACYPI008757 GB15658 AGAP007683 AAEL013386 PB25194 PHUM550160 GLEAN_08602
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG5625 (FlyBase Curators, 2008, Port et al., 2008, Korolchuk et al., 2007, Belenkaya et al., 2008, Cammarato et al., 2011) Dvps35 (Belenkaya et al., 2008, Port et al., 2008) Vps35 (Pocha et al., 2011, Lin et al., 2008, Franch-Marro et al., 2008, ) vps35 (Korolchuk et al., 2007, Belenkaya et al., 2008)
Name Synonym	CG5625   Vacuolar protein sorting 35
Secondary FlyBase IDs
References ( 17 )
Research paper	Pocha et al., 2011, Curr. Biol. 21(13): 1111--1117 Retromer controls epithelial cell polarity by trafficking the apical determinant crumbs. [FBrf0214250] Belenkaya et al., 2008, Dev. Cell 14(1): 120--131 The retromer complex influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network. [FBrf0203062] Franch-Marro et al., 2008, Nat. Cell Biol. 10(2): 170--177 Wingless secretion requires endosome-to-Golgi retrieval of Wntless/Evi/Sprinter by the retromer complex. [FBrf0202912] Port et al., 2008, Nat. Cell Biol. 10(2): 178--185 Wingless secretion promotes and requires retromer-dependent cycling of Wntless. [FBrf0202681] Korolchuk et al., 2007, J. Cell Sci. 120(24): 4367--4376 Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation. [FBrf0202367]
Supplementary material	Cammarato et al., 2011, PLoS ONE 6(4): Table S1. Proteins & Peptides. [FBrf0214682] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] BDGP TI submission, 2006.4.12, Batch655.xml Batch655.xml [FBrf0200401] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
Abstract	Lin et al., 2008, A. Dros. Res. Conf. 49: 390C The retromer complex is involved in Wingless/Wnt secretion by controlling retrograde transport of Wntless from endosomes to the trans Golgi network. [FBrf0204049]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]