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Gene Dmel\rad50

General Information
Symbol	Dmel\rad50	Species	D. melanogaster
Name	rad50	Annotation symbol	CG6339
Feature type	protein_coding_gene	FlyBase ID	FBgn0034728
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	58E1-58E1	Sequence location	2R:18,308,544..18,312,979 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene rad50 is referred to in FlyBase by the symbol Dmel\rad50 (CG6339, FBgn0034728). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: nuclease activity; zinc ion binding; ATP binding. There is experimental evidence that it is involved in the biological process: instar larval development; response to DNA damage stimulus; telomere maintenance; chromosome organization; telomere capping. 6 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; eye; wing disc; chromosome, telomeric region; chaeta; condensed chromosome; wing. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Haemolysin E; Rad50 zinc hook; Recombination/repair protein Rad50; Zinc hook, Rad50. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, larval trachea, adult female reproductive system, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1638887_a_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of rad50. Gene sequence location is 2R:18308544..18312979.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN20510 HFA04523
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 58E1-58E1   Limits computationally determined from genome sequence between P{lacW}l(2)k13211k13211&P{EP}EP827 and P{PZ}l(2)rG270rG270
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\rad50 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) rad50-RD FBtr0301668  NM_166533   4034   1318 rad50-RE FBtr0305323     4034   1318
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein rad50-PD   FBpp0290882   152.2   1318   7.63     NP_726199   AAF46847 rad50-PE   FBpp0293866   152.1   1318   7.72
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Recombination/repair protein Rad50 (IPR004584) Rad50 zinc hook (IPR007517) Zinc hook, Rad50 (IPR013134) Haemolysin E (IPR010356)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF46847   AI295594   AI533555   AW944415   AY052106 AAK93530   BI142152   BI638226   BT003555 AAO39559   BT100311   CO331372   CZ478607   EC216466   EC223707   EV595075   FL502413   FL502649
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W252
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0034728    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0034728 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0034728 expression level Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1692) embryo 02-04hr  (473) embryo 04-06hr  (993) embryo 06-08hr  (692) embryo 08-10hr  (496) embryo 10-12hr  (214) embryo 12-14hr  (188) embryo 14-16hr    95 embryo 16-18hr  (117) embryo 18-20hr  103 embryo 20-22hr    94 embryo 22-24hr  (146) larva L1  (199) larva L2  (228) larva L3 12hr old  (213) larva L3 puffstage 1-2  (376) larva L3 puffstage 3-6  (473) larva L3 puffstage 7-9  (490) white prepupae new  (418) white prepupae 12hr  (456) white prepupae 24hr  (453) pupae 2d postWPP  (225) pupae 3d postWPP  (134) pupae 4d postWPP  (115) adult male 01day  (262) adult male 05day  (324) adult male 30day  (378) adult female 01day  (670) adult female 05day  (1610) adult female 30day  (1502) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1692) embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day  (1610) adult female 30day  1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0034728 expression level Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1692) embryo 02-04hr  (473) embryo 04-06hr  (993) embryo 06-08hr  (692) embryo 08-10hr  (496) embryo 10-12hr  (214) embryo 12-14hr  (188) embryo 14-16hr    95 embryo 16-18hr  117 embryo 18-20hr  103 embryo 20-22hr    94 embryo 22-24hr  (146) larva L1  (199) larva L2  (228) larva L3 12hr old  (213) larva L3 puffstage 1-2  (376) larva L3 puffstage 3-6  (473) larva L3 puffstage 7-9  (490) white prepupae new  (418) white prepupae 12hr  (456) white prepupae 24hr  (453) pupae 2d postWPP  (225) pupae 3d postWPP  134 pupae 4d postWPP  115 adult male 01day  (262) adult male 05day  (324) adult male 30day  (378) adult female 01day  (670) adult female 05day  (1610) adult female 30day  (1502) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day  1610 adult female 30day  1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1692 embryo 02-04hr    473 embryo 04-06hr    993 embryo 06-08hr    692 embryo 08-10hr    496 embryo 10-12hr    214 embryo 12-14hr    188 embryo 14-16hr    95 embryo 16-18hr    117 embryo 18-20hr    103 embryo 20-22hr    94 embryo 22-24hr    146 larva L1    199 larva L2    228 larva L3 12hr old    213 larva L3 puffstage 1-2    376 larva L3 puffstage 3-6    473 larva L3 puffstage 7-9    490 white prepupae new    418 white prepupae 12hr    456 white prepupae 24hr    453 pupae 2d postWPP    225 pupae 3d postWPP    134 pupae 4d postWPP    115 adult male 01day    262 adult male 05day    324 adult male 30day    378 adult female 01day    670 adult female 05day    1610 adult female 30day    1502 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0034728    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0034728 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, larval trachea, adult female reproductive system, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0034728 expression level Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0034728 expression level Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    233.1 Larval Midgut    46 Larval Hindgut    108.8 Larval Malpighian Tubules    92.7 Larval Fat Body    65.1 Larval Salivary Gland    62.7 Larval Trachea    267.775 Larval Carcass    73.65 Adult Head    90.2 Adult Eye    127.175 Adult Brain    109.9 Adult Thoracic-Abdominal Ganglion    117.2 Adult Crop    127.9 Adult Midgut    98.8 Adult Hindgut    140.8 Adult Malpighian Tubules    158.1 Adult Fat Body    80.2 Adult Salivary Gland    82.7 Adult Heart    91.7 Adult VirginFemale Spermatheca    173.8 Adult InseminatedFemale Spermatheca    152.1 Adult Ovary    333 Adult Testis    46.2 Adult Male Accessory Gland    212.8 Adult Carcass    82.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    90.2   NA  Eye    127.175   NA  Brain    109.9   233.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    117.2   NA  Crop    127.9   46  Midgut    98.8   108.8  Hindgut    140.8   92.7  Malpighian Tubules    158.1   65.1  Fat Body    80.2   62.7  Salivary Gland    82.7   NA  Heart    91.7   267.775  Trachea    NA   NA  VirginFemale Spermatheca    173.8   NA  InseminatedFemale Spermatheca    152.1   NA  Ovary    333   NA  Testis    46.2   NA  Male Accessory Gland    212.8   73.65  Carcass    82.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034728 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6339-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage rad50EP1 lethal | pupal stage | recessive rad50Δ5.1 lethal | recessive rad50EP1 viable, with Scer\GAL4pnr-MD237 rad50GD4104 Other Phenotypes Allele increased cell death rad50Δ5.1 mitotic cell cycle defective rad50Δ5.1 mitotic cell cycle defective (with Df(2R)X58-8) rad50EP1 radiation sensitive rad50Δ5.1 Phenotype manifest in Allele chaeta rad50Δ5.1 chromosome, telomeric region rad50Δ5.1 chromosome, telomeric region (with Df(2R)X58-8) rad50EP1 condensed chromosome rad50Δ5.1 eye rad50Δ5.1 mitosis & nuclear chromosome rad50Δ5.1 mitosis & nuclear chromosome (with Df(2R)X58-8) rad50EP1 wing rad50Δ5.1 wing disc rad50Δ5.1 wing margin (with rad50EP1) rad50Δ5.1 wing margin (with rad50Δ5.1) rad50EP1
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of rad50 Class Mutagen Stocks Known lesion rad50EP1 amorphic allele - genetic evidence P-element activity 1 Yes rad50e00761 piggyBac transposase 1 -- rad50Δ5.1 amorphic allele - genetic evidence P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of rad50 Class Mutagen Stocks Known lesion rad50GD4104 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rad50KK100414 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rad50+tCa in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7173 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4104} NA P{KK100414} NA characterization construct P{rad50+tCa} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}mRpS29EP2600 NA P{EP}rad50EP1 NA miscellaneous insertions P{SUPor-P}KG02493 NA P{UASp-YFP.Rab23.S51N}mRpS2902 NA PBac{RB}rad50e00761 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome organization inferred from mutant phenotype (Ciapponi et al., 2004, Ciapponi et al., 2006) instar larval development inferred from mutant phenotype (Gorski and Eeken, 2002) response to DNA damage stimulus inferred from mutant phenotype (Ravi et al., 2009) telomere capping inferred from mutant phenotype (Ciapponi et al., 2006) telomere maintenance inferred from mutant phenotype (Ciapponi et al., 2004)
Cellular Component
CV term Evidence References
nucleus inferred from mutant phenotype (Ciapponi et al., 2006)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR004584, InterPro:IPR007517 (FlyBase Curators et al., 2004-) nuclease activity inferred from electronic annotation with InterPro:IPR007517 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR007517 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA repair inferred from sequence or structural similarity with Saccharomyces RAD50 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
Mre11 complex inferred from electronic annotation with InterPro:IPR004584 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions rad50 allele Gene References unspecified mei-41 (Ciapponi et al., 2006) nbs (Ciapponi et al., 2006) tefu (Ciapponi et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63180 DroID - A comprehensive database of gene and protein interactions. • FBgn0034728 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37564
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11892 Dere\GG22190 Dgri\GH20438 Dmoj\GI19621 Dper\GL17546 Dpse\GA19522 Dsec\GM15911 Dsim\GD11670 Dvir\GJ14987 Dwil\GK20820 Dyak\GE14184
Curated drosophilid orthologs	Dpse\GA19522   Dsim\rad50 (Anderson et al., 2009)
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18538 LH15600 LH17478 ISCW015559 FBgn0231806 FBgn0222816 FBgn0202187 FBgn0183410 FBgn0170827 JGI_V11_322547 FBgn0079518 FBgn0155149 FBgn0142358 FBgn0127901 FBgn0114364 FBgn0088932 CPIJ004115 BGIBMGA005449 ACYPI43058 ACYPI009938 GB15340 AGAP003676 AAEL014748 AAEL005245 PB20308 PHUM549380 GLEAN_15093
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	8523 w1118; P{EP}rad50EP1/CyO
Harvard	- PBac{RB}rad50e00761
VDRC	v103394 P{KK100414}VIE-260B v15879 w1118; P{GD4104}v15879
Genomic Clones ( 1 )
	BACR48M13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 10 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP09268 SD05424
Other clones	FI13085
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD16158 SD20460
Other clones	64962 272785 Dmel_neb_014527_0621_2143 DMG4-chr2R.31.011.pred.a EK299427
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0034728 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37564
Antibody Information
	polyclonal (Gao et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: rad50 CG6339 (Gorski and Eeken, 2002.3.21)
Source for database merge of
Additional comments
Other Comments
	Mutants show high levels of spontaneous telomere fusion and chromosome breakage in dividing cells. (Ciapponi et al., 2004) rad50-deficient larvae are not sensitive to ionizing radiation, methyl methanesulfonate (MMS) and the cross-linking, bifunctional nitrogen mustard - Mechlorethamine (MEC). Tergites, failing to develop properly, are the cause of severe impairment of the segmentation of the fly abdomen of rad50 deficient flies. Eyes and wings of rad50-/- flies are irregularly shaped. rad50 mutants show elevated levels of anaphase bridges in dividing cells of the brains and the imaginal discs of the third instar larvae. (Gorski and Eeken, 2002)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_166533 RefSeq (Proteins) • NP_726199 DDBJ / EMBL / GenBank • AAF46847 AAK93530 AAO39559 AI295594 AI533555 AW944415 AY052106 BT003555 CZ478607 Entrez Gene - A searchable database of RefSeq genes. • 37564 UniProtKB/TrEMBL • Q9W252
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Recombination/repair protein Rad50 (IPR004584) Rad50 zinc hook (IPR007517) Zinc hook, Rad50 (IPR013134) Haemolysin E (IPR010356)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63180 DroID - A comprehensive database of gene and protein interactions. • FBgn0034728 DRSC - Results from RNAi screens. • FBgn0034728 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034728 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6339-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0034728 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37564 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37564 modMine - Data generated by the modENCODE project. • FBgn0034728 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18538 LH15600 LH17478 ISCW015559 FBgn0231806 FBgn0222816 FBgn0202187 FBgn0183410 FBgn0170827 JGI_V11_322547 FBgn0079518 FBgn0155149 FBgn0142358 FBgn0127901 FBgn0114364 FBgn0088932 CPIJ004115 BGIBMGA005449 ACYPI43058 ACYPI009938 GB15340 AGAP003676 AAEL014748 AAEL005245 PB20308 PHUM549380 GLEAN_15093
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	6339 (Ravi et al., 2009) CG6339 (Huet et al., 2002, Keleman et al., 2009.8.5) Dmrad50 (Gorski and Eeken, 2002) Rad50 (Mason et al., 2004, Purdy and Su, 2004, Holmes et al., 2006, Hochwagen, 2006, Gao et al., 2008, Brodsky et al., 2004, Gao et al., 2009, Gao et al., 2009) rad50 (Raffa et al., 2005, Oikemus et al., 2007, Ciapponi et al., 2006, Zhang et al., 2007, Cenci et al., 2008, Oikemus et al., 2006, Anderson et al., 2009, Akdemir et al., 2007, Musarò et al., 2008, Gao et al., 2009, Morciano et al., 2009, Reis et al., 2011) RAD50 (Cenci et al., 2005, Rimkus et al., 2008, Dronamraju and Mason, 2009)
Name Synonym	rad50
Secondary FlyBase IDs
References ( 46 )
Generate a list of	All references relating to this gene ( 46 )
List References by type	Research paper  ( 19 ) Supplementary material  ( 5 ) Review  ( 3 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 8 ) FlyBase analysis  ( 1 ) Computer file  ( 3 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 2 )
	Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...