FB2021_01, released Feb 18, 2021

Gene: Dmel\Ice1

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General Information
Symbol
Dmel\Ice1
Species
D. melanogaster
Name
Interacts with the C terminus of ELL 1
Annotation Symbol
CG13550
Feature Type
protein_coding_gene
FlyBase ID
FBgn0034853
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Key Links
Genomic Location
Cytogenetic map
59D9-59D9
Sequence location
2R:23,366,866..23,371,459 [+]
Recombination map

2-102

RefSeq locus
NT_033778 REGION:23366866..23371459
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Smith et al., 2011)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in snRNA transcription by RNA polymerase II
inferred from mutant phenotype
(assigned by UniProt )
(Hu et al., 2013)
inferred from mutant phenotype
(Smith et al., 2011)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of super elongation complex
inferred from physical interaction with FLYBASE:Su(Tpl); FB:FBgn0014037
(Smith et al., 2011)
part_of transcription elongation factor complex
inferred from direct assay
(assigned by UniProt )
(Smith et al., 2011)
located_in transcriptionally active chromatin
inferred from mutant phenotype
(assigned by UniProt )
(Hu et al., 2013)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the ICE1 family. (Q9W1R4)
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically acts as a scaffold protein that promotes the LEC complex formation and recruitment and RNA polymerase II occupancy at snRNA genes in subnuclear bodies (PubMed:23932780).
    (UniProt, Q9W1R4)
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\Ice1 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.52

    Sequence Ontology: Class of Gene
    gene_with_edited_transcript
    (Graveley et al., 2011)
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Ice1-RA
    FBtr0072010
    NM_137942
    4537
    1395
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Ice1-PA
    FBpp0071919
    157.5
    1395
    5.08
    NP_611786
    AAF46989
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Component of the little elongation complex (LEC), at least composed of Ell, Eaf, Ice1 and Ice2.

    (UniProt, Q9W1R4)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ice1 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 3
    organism

    Comment: maternally deposited

    (Fisher et al., 2012)
    embryonic stage 4 -- 6
    organism
    (Fisher et al., 2012)
    embryonic stage 7 -- 8
    organism | faint | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 9 -- 10
    organism | faint | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 13 -- 16
    embryonic/larval muscle system
    (Fisher et al., 2012)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    mass spectroscopy
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    day 5 of adulthood & male -- day 7 of adulthood & male
    spermatozoon
    (Wasbrough et al., 2010)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    part_of super elongation complex
    inferred from physical interaction with FLYBASE:Su(Tpl); FB:FBgn0014037
    (Smith et al., 2011)
    part_of transcription elongation factor complex
    inferred from direct assay
    (assigned by UniProt )
    (Smith et al., 2011)
    located_in transcriptionally active chromatin
    inferred from mutant phenotype
    (assigned by UniProt )
    (Hu et al., 2013)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Ice1 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 2 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 4 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Ice1
    Transgenic constructs containing regulatory region of Ice1
    Aberrations (Deficiencies and Duplications) ( 1 )
    Inferred from experimentation
    Gene disrupted in
    Df(2R)Exel7178
    (Ryder, 2004.4.26)
    Inferred from location
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919016E )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00002979
    Drosophila simulans
    Dsim\GD11757
    Drosophila sechellia
    Dsec\GM16006
    Drosophila erecta
    Dere\GG22847
    Drosophila yakuba
    Dyak\GE14283
    Drosophila ananassae
    Dana\GF11855
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA12356
    Drosophila persimilis
    Dper\GL17568
    Drosophila willistoni
    Dwil\GK23062
    Drosophila virilis
    Dvir\GJ19991
    Drosophila mojavensis
    Dmoj\GI19025
    Drosophila grimshawi
    Dgri\GH20662
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502Q4 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17023894
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY010467
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_12111
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ004596
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02HL )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Acyrthosiphon pisum
    Pea aphid
    Apim\ACYPI25726
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02F2 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Smar\SMAR009472
    Strigamia maritima
    European centipede
    Smar\SMAR014181
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur06g06670
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur291g00010
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur24g01430
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur19g01740
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur01g04620
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Ice1 - CG8229
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Smith et al., 2011)
      Ice1 - Eaf
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Smith et al., 2011)
      Ice1 - Ice2
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Smith et al., 2011)
      Ice1 - Su(Tpl)
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Smith et al., 2011)
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Component of the little elongation complex (LEC), at least composed of Ell, Eaf, Ice1 and Ice2.
      (UniProt, Q9W1R4 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2R
      Recombination map

      2-102

      Cytogenetic map
      59D9-59D9
      Sequence location
      2R:23,366,866..23,371,459 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      59D9-59D9
      Limits computationally determined from genome sequence between P{lacW}l(2)k07136k07136 and P{lacW}l(2)s4830s4830&P{lacW}chrwk06908
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-102

      (FlyBase, 2016)

      2-107.0

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (3)
      Genomic Clones (20)
      cDNA Clones (15)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Smith et al., 2011)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Ice1 CG13550

      (Smith et al., 2011)
      Source for database merge of
      Additional comments
      Other Comments

      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and a spindle that is aberrantly long when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

      (Goshima et al., 2007)

      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

      (Boutros et al., 2004)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 23 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (5)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (31)