FlyBase Gene Report: Dmel\pita

General Information

Symbol

Dmel\pita

Species

D. melanogaster

Name

pita

Annotation Symbol

CG3941

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034878

Gene Model Status

Current

Stock Availability

18 publicly available

Gene Summary

pita (pita) encodes an architectural chromatin protein essential for embryonic development that organizes open chromatin in the regulatory regions (predominantly promoters). pita product functions include enhancer blocking, support of distant interactions, protection from spreading of repressed chromatin and recruitment of transcription factors. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

spdk

Key Links

Genomic Location

Cytogenetic map

59E3-59E3

Sequence location

2R:23,548,967..23,554,712 [-]

Recombination map

2-103

RefSeq locus

NT_033778 REGION:23548967..23554712

Sequence

Get Decorated FASTA

Genomic Maps

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from direct assay

(Maksimenko et al., 2015)

enables chromatin insulator sequence binding

inferred from direct assay

(Maksimenko et al., 2015)

enables DNA binding

inferred from direct assay

(Page et al., 2005)

enables protein binding

inferred from physical interaction with FLYBASE:Cp190; FB:FBgn0000283

(Maksimenko et al., 2015)

enables sequence-specific DNA binding

inferred from direct assay

(Maksimenko et al., 2015)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(assigned by GO_Central )

(Gaudet et al., 2011)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR012934

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in DNA endoreduplication

inferred from mutant phenotype

(Page et al., 2005)

involved_in DNA replication

inferred from mutant phenotype

(Page et al., 2005)

involved_in regulation of transcription, DNA-templated

inferred from direct assay

(Maksimenko et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Page et al., 2005)

located_in polytene chromosome

inferred from direct assay

(Maksimenko et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Summary (Interactive Fly)

zinc finger transcription factor that serves as an activator of expression of a set of genes required for initiation of DNA replication

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\pita for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q95RQ8)

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Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

pita-RA

FBtr0072112

NM_137962

2472

683

pita-RB

FBtr0072113

NM_166607

2529

623

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

pita-PA

FBpp0072021

78.3

683

7.30

NP_611806

AAF47026

pita-PB

FBpp0072022

71.6

623

7.31

NP_726339

AAF47025

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pita using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | intense | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium